Morphology and immunolocalization of aquaporins 1 and 9 in the agouti (Dasyprocta azarae) testis excurrent ducts
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/1984-3143-AR2021-0070 http://hdl.handle.net/11449/233954 |
Resumo: | This study investigated the morphology and immunoexpression of aquaporins (AQPs) 1 and 9 in the rete testis, efferent ducts, epididymis, and vas deferens in the Azara's agouti (Dasyprocta azarae). For this purpose, ten adult sexually mature animals were used in histologic and immunohistochemical analyses. The Azara's agouti rete testis was labyrinthine and lined with simple cubic epithelium. Ciliated and non-ciliated cells were observed in the epithelium of the efferent ducts. The epididymal cellular population was composed of principal, basal, apical, clear, narrow, and halo cells. The epithelium lining of vas deferens was composed of the principal and basal cells. AQPs 1 and 9 were not expressed in the rete testis. Positive reaction to AQP1 was observed at the luminal border of non-ciliated cells of the efferent ducts, and in the peritubular stroma and blood vessels in the epididymis, and vas deferens. AQP9 was immunolocalized in the epithelial cells in the efferent ducts, epididymis and vas deferens. The morphology of Azara's agouti testis excurrent ducts is similar to that reported for other rodents such as Cuniculus paca. The immunolocalization results of the AQPs suggest that the expression of AQPs is species-specific due to differences in localization and expression when compared to studies in other mammals species. The knowledge about the expression of AQPs in Azara's agouti testis excurrent ducts is essential to support future reproductive studies on this animal, since previous studies show that AQPs may be biomarkers of male fertility and infertility. |
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Morphology and immunolocalization of aquaporins 1 and 9 in the agouti (Dasyprocta azarae) testis excurrent ductsaquaporinsefferent ductsepididymismorphologyrodentsThis study investigated the morphology and immunoexpression of aquaporins (AQPs) 1 and 9 in the rete testis, efferent ducts, epididymis, and vas deferens in the Azara's agouti (Dasyprocta azarae). For this purpose, ten adult sexually mature animals were used in histologic and immunohistochemical analyses. The Azara's agouti rete testis was labyrinthine and lined with simple cubic epithelium. Ciliated and non-ciliated cells were observed in the epithelium of the efferent ducts. The epididymal cellular population was composed of principal, basal, apical, clear, narrow, and halo cells. The epithelium lining of vas deferens was composed of the principal and basal cells. AQPs 1 and 9 were not expressed in the rete testis. Positive reaction to AQP1 was observed at the luminal border of non-ciliated cells of the efferent ducts, and in the peritubular stroma and blood vessels in the epididymis, and vas deferens. AQP9 was immunolocalized in the epithelial cells in the efferent ducts, epididymis and vas deferens. The morphology of Azara's agouti testis excurrent ducts is similar to that reported for other rodents such as Cuniculus paca. The immunolocalization results of the AQPs suggest that the expression of AQPs is species-specific due to differences in localization and expression when compared to studies in other mammals species. The knowledge about the expression of AQPs in Azara's agouti testis excurrent ducts is essential to support future reproductive studies on this animal, since previous studies show that AQPs may be biomarkers of male fertility and infertility.Departamento de Anatomia Universidade Estadual PaulistaDepartamento de Anatomia Universidade Estadual de LondrinaDepartamento de Morfologia e Fisiologia Animal Universidade Estadual PaulistaDepartamento de Anatomia Universidade Estadual PaulistaDepartamento de Morfologia e Fisiologia Animal Universidade Estadual PaulistaUniversidade Estadual Paulista (UNESP)Universidade Estadual de Londrina (UEL)Schimming, Bruno Cesar [UNESP]Martins, Leandro Luisde Oliveira, Fabrício Singaretti [UNESP]Pinheiro, Patrícia Fernanda Felipe [UNESP]Domeniconi, Raquel Fantin [UNESP]2022-05-01T11:54:09Z2022-05-01T11:54:09Z2021-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1-16http://dx.doi.org/10.1590/1984-3143-AR2021-0070Animal Reproduction, v. 18, n. 3, p. 1-16, 2021.1984-31431806-9614http://hdl.handle.net/11449/23395410.1590/1984-3143-AR2021-00702-s2.0-85122028168Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengAnimal Reproductioninfo:eu-repo/semantics/openAccess2022-05-01T11:54:09Zoai:repositorio.unesp.br:11449/233954Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462022-05-01T11:54:09Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Morphology and immunolocalization of aquaporins 1 and 9 in the agouti (Dasyprocta azarae) testis excurrent ducts |
title |
Morphology and immunolocalization of aquaporins 1 and 9 in the agouti (Dasyprocta azarae) testis excurrent ducts |
spellingShingle |
Morphology and immunolocalization of aquaporins 1 and 9 in the agouti (Dasyprocta azarae) testis excurrent ducts Schimming, Bruno Cesar [UNESP] aquaporins efferent ducts epididymis morphology rodents |
title_short |
Morphology and immunolocalization of aquaporins 1 and 9 in the agouti (Dasyprocta azarae) testis excurrent ducts |
title_full |
Morphology and immunolocalization of aquaporins 1 and 9 in the agouti (Dasyprocta azarae) testis excurrent ducts |
title_fullStr |
Morphology and immunolocalization of aquaporins 1 and 9 in the agouti (Dasyprocta azarae) testis excurrent ducts |
title_full_unstemmed |
Morphology and immunolocalization of aquaporins 1 and 9 in the agouti (Dasyprocta azarae) testis excurrent ducts |
title_sort |
Morphology and immunolocalization of aquaporins 1 and 9 in the agouti (Dasyprocta azarae) testis excurrent ducts |
author |
Schimming, Bruno Cesar [UNESP] |
author_facet |
Schimming, Bruno Cesar [UNESP] Martins, Leandro Luis de Oliveira, Fabrício Singaretti [UNESP] Pinheiro, Patrícia Fernanda Felipe [UNESP] Domeniconi, Raquel Fantin [UNESP] |
author_role |
author |
author2 |
Martins, Leandro Luis de Oliveira, Fabrício Singaretti [UNESP] Pinheiro, Patrícia Fernanda Felipe [UNESP] Domeniconi, Raquel Fantin [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Universidade Estadual de Londrina (UEL) |
dc.contributor.author.fl_str_mv |
Schimming, Bruno Cesar [UNESP] Martins, Leandro Luis de Oliveira, Fabrício Singaretti [UNESP] Pinheiro, Patrícia Fernanda Felipe [UNESP] Domeniconi, Raquel Fantin [UNESP] |
dc.subject.por.fl_str_mv |
aquaporins efferent ducts epididymis morphology rodents |
topic |
aquaporins efferent ducts epididymis morphology rodents |
description |
This study investigated the morphology and immunoexpression of aquaporins (AQPs) 1 and 9 in the rete testis, efferent ducts, epididymis, and vas deferens in the Azara's agouti (Dasyprocta azarae). For this purpose, ten adult sexually mature animals were used in histologic and immunohistochemical analyses. The Azara's agouti rete testis was labyrinthine and lined with simple cubic epithelium. Ciliated and non-ciliated cells were observed in the epithelium of the efferent ducts. The epididymal cellular population was composed of principal, basal, apical, clear, narrow, and halo cells. The epithelium lining of vas deferens was composed of the principal and basal cells. AQPs 1 and 9 were not expressed in the rete testis. Positive reaction to AQP1 was observed at the luminal border of non-ciliated cells of the efferent ducts, and in the peritubular stroma and blood vessels in the epididymis, and vas deferens. AQP9 was immunolocalized in the epithelial cells in the efferent ducts, epididymis and vas deferens. The morphology of Azara's agouti testis excurrent ducts is similar to that reported for other rodents such as Cuniculus paca. The immunolocalization results of the AQPs suggest that the expression of AQPs is species-specific due to differences in localization and expression when compared to studies in other mammals species. The knowledge about the expression of AQPs in Azara's agouti testis excurrent ducts is essential to support future reproductive studies on this animal, since previous studies show that AQPs may be biomarkers of male fertility and infertility. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-01-01 2022-05-01T11:54:09Z 2022-05-01T11:54:09Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/1984-3143-AR2021-0070 Animal Reproduction, v. 18, n. 3, p. 1-16, 2021. 1984-3143 1806-9614 http://hdl.handle.net/11449/233954 10.1590/1984-3143-AR2021-0070 2-s2.0-85122028168 |
url |
http://dx.doi.org/10.1590/1984-3143-AR2021-0070 http://hdl.handle.net/11449/233954 |
identifier_str_mv |
Animal Reproduction, v. 18, n. 3, p. 1-16, 2021. 1984-3143 1806-9614 10.1590/1984-3143-AR2021-0070 2-s2.0-85122028168 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Animal Reproduction |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1-16 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1797789333079457792 |