Effects of calcium hypochlorite and octenidine hydrochloride on L929 and human periodontal ligament cells
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/0103-6440201902280 http://hdl.handle.net/11449/189288 |
Resumo: | The aim of this study was to assess cytotoxicity and cell migration of calcium hypochlorite [Ca(OCl)2] and octenidine hydrochloride-OCT (Octenisept®, Schülke & Mayr, Norderstedt, Germany) in L929 and human periodontal ligament (hPDL) cells. The cells were exposed to different doses of different solutions: 2.5% and 5% Ca(OCl)2, 0.1% OCT, 2.5% NaOCl and 2% CHX for 10 min. Cell viability was assessed by methyl-thiazol-tetrazolium (MTT) and neutral red (NR) assays, and cell migration was determined by wound-healing assay. Statistical analysis was performed by two-way ANOVA and Bonferroni tests (α=0.05). The MTT and NR assays revealed that 0.1% OCT was less cytotoxic in hPDL cells (p<0.05), followed by 2% CHX and 2.5% Ca(OCl)2 (p<0.05). There was no significant difference between 2.5% NaOCl and 5% Ca(OCl)2 (p>0.05), but these solutions showed greater cytotoxicity than the others. The result was the same for L929 cells, except that there was no significant difference between 2% CHX and 2.5% Ca(OCl)2 (p>0.05). Wound-healing assay in L929 and hPDL cells showed that cell migration of 0.1% OCT, 2% CHX and 2.5% Ca(OCl)2 groups was higher than 5% Ca(OCl)2 and 2.5% NaOCl groups at 24 h (p<0.05). In conclusion, 0.1% OCT had lower cytotoxicity in tested cell lines than CHX, Ca(OCl)2 and NaOCl. Cell migration was higher for 0.1% OCT, 2% CHX and 2.5% Ca(OCl)2. Therefore, in terms of cytotoxicity, OCT and Ca(OCl)2 have the potential to be used as root canal irrigants. |
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Effects of calcium hypochlorite and octenidine hydrochloride on L929 and human periodontal ligament cellsCalcium hypochloriteCytotoxicityFibroblastsOctenidineRoot canal treatmentThe aim of this study was to assess cytotoxicity and cell migration of calcium hypochlorite [Ca(OCl)2] and octenidine hydrochloride-OCT (Octenisept®, Schülke & Mayr, Norderstedt, Germany) in L929 and human periodontal ligament (hPDL) cells. The cells were exposed to different doses of different solutions: 2.5% and 5% Ca(OCl)2, 0.1% OCT, 2.5% NaOCl and 2% CHX for 10 min. Cell viability was assessed by methyl-thiazol-tetrazolium (MTT) and neutral red (NR) assays, and cell migration was determined by wound-healing assay. Statistical analysis was performed by two-way ANOVA and Bonferroni tests (α=0.05). The MTT and NR assays revealed that 0.1% OCT was less cytotoxic in hPDL cells (p<0.05), followed by 2% CHX and 2.5% Ca(OCl)2 (p<0.05). There was no significant difference between 2.5% NaOCl and 5% Ca(OCl)2 (p>0.05), but these solutions showed greater cytotoxicity than the others. The result was the same for L929 cells, except that there was no significant difference between 2% CHX and 2.5% Ca(OCl)2 (p>0.05). Wound-healing assay in L929 and hPDL cells showed that cell migration of 0.1% OCT, 2% CHX and 2.5% Ca(OCl)2 groups was higher than 5% Ca(OCl)2 and 2.5% NaOCl groups at 24 h (p<0.05). In conclusion, 0.1% OCT had lower cytotoxicity in tested cell lines than CHX, Ca(OCl)2 and NaOCl. Cell migration was higher for 0.1% OCT, 2% CHX and 2.5% Ca(OCl)2. Therefore, in terms of cytotoxicity, OCT and Ca(OCl)2 have the potential to be used as root canal irrigants.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Department of Restorative Dentistry Araraquara School of Dentistry UNESP – Universidade Estadual PaulistaDepartment of Restorative Dentistry Araraquara School of Dentistry UNESP – Universidade Estadual PaulistaUniversidade Estadual Paulista (Unesp)Coaguila-Llerena, Hernán [UNESP]Rodrigues, Elisandra Márcia [UNESP]Tanomaru-Filho, Mário [UNESP]Guerreiro-Tanomaru, Juliane Maria [UNESP]Faria, Gisele [UNESP]2019-10-06T16:35:55Z2019-10-06T16:35:55Z2019-05-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article213-219application/pdfhttp://dx.doi.org/10.1590/0103-6440201902280Brazilian Dental Journal, v. 30, n. 3, p. 213-219, 2019.1806-47600103-6440http://hdl.handle.net/11449/18928810.1590/0103-6440201902280S0103-644020190003002132-s2.0-85067516519S0103-64402019000300213.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian Dental Journalinfo:eu-repo/semantics/openAccess2023-12-03T06:14:37Zoai:repositorio.unesp.br:11449/189288Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-12-03T06:14:37Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Effects of calcium hypochlorite and octenidine hydrochloride on L929 and human periodontal ligament cells |
title |
Effects of calcium hypochlorite and octenidine hydrochloride on L929 and human periodontal ligament cells |
spellingShingle |
Effects of calcium hypochlorite and octenidine hydrochloride on L929 and human periodontal ligament cells Coaguila-Llerena, Hernán [UNESP] Calcium hypochlorite Cytotoxicity Fibroblasts Octenidine Root canal treatment |
title_short |
Effects of calcium hypochlorite and octenidine hydrochloride on L929 and human periodontal ligament cells |
title_full |
Effects of calcium hypochlorite and octenidine hydrochloride on L929 and human periodontal ligament cells |
title_fullStr |
Effects of calcium hypochlorite and octenidine hydrochloride on L929 and human periodontal ligament cells |
title_full_unstemmed |
Effects of calcium hypochlorite and octenidine hydrochloride on L929 and human periodontal ligament cells |
title_sort |
Effects of calcium hypochlorite and octenidine hydrochloride on L929 and human periodontal ligament cells |
author |
Coaguila-Llerena, Hernán [UNESP] |
author_facet |
Coaguila-Llerena, Hernán [UNESP] Rodrigues, Elisandra Márcia [UNESP] Tanomaru-Filho, Mário [UNESP] Guerreiro-Tanomaru, Juliane Maria [UNESP] Faria, Gisele [UNESP] |
author_role |
author |
author2 |
Rodrigues, Elisandra Márcia [UNESP] Tanomaru-Filho, Mário [UNESP] Guerreiro-Tanomaru, Juliane Maria [UNESP] Faria, Gisele [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Coaguila-Llerena, Hernán [UNESP] Rodrigues, Elisandra Márcia [UNESP] Tanomaru-Filho, Mário [UNESP] Guerreiro-Tanomaru, Juliane Maria [UNESP] Faria, Gisele [UNESP] |
dc.subject.por.fl_str_mv |
Calcium hypochlorite Cytotoxicity Fibroblasts Octenidine Root canal treatment |
topic |
Calcium hypochlorite Cytotoxicity Fibroblasts Octenidine Root canal treatment |
description |
The aim of this study was to assess cytotoxicity and cell migration of calcium hypochlorite [Ca(OCl)2] and octenidine hydrochloride-OCT (Octenisept®, Schülke & Mayr, Norderstedt, Germany) in L929 and human periodontal ligament (hPDL) cells. The cells were exposed to different doses of different solutions: 2.5% and 5% Ca(OCl)2, 0.1% OCT, 2.5% NaOCl and 2% CHX for 10 min. Cell viability was assessed by methyl-thiazol-tetrazolium (MTT) and neutral red (NR) assays, and cell migration was determined by wound-healing assay. Statistical analysis was performed by two-way ANOVA and Bonferroni tests (α=0.05). The MTT and NR assays revealed that 0.1% OCT was less cytotoxic in hPDL cells (p<0.05), followed by 2% CHX and 2.5% Ca(OCl)2 (p<0.05). There was no significant difference between 2.5% NaOCl and 5% Ca(OCl)2 (p>0.05), but these solutions showed greater cytotoxicity than the others. The result was the same for L929 cells, except that there was no significant difference between 2% CHX and 2.5% Ca(OCl)2 (p>0.05). Wound-healing assay in L929 and hPDL cells showed that cell migration of 0.1% OCT, 2% CHX and 2.5% Ca(OCl)2 groups was higher than 5% Ca(OCl)2 and 2.5% NaOCl groups at 24 h (p<0.05). In conclusion, 0.1% OCT had lower cytotoxicity in tested cell lines than CHX, Ca(OCl)2 and NaOCl. Cell migration was higher for 0.1% OCT, 2% CHX and 2.5% Ca(OCl)2. Therefore, in terms of cytotoxicity, OCT and Ca(OCl)2 have the potential to be used as root canal irrigants. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-06T16:35:55Z 2019-10-06T16:35:55Z 2019-05-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/0103-6440201902280 Brazilian Dental Journal, v. 30, n. 3, p. 213-219, 2019. 1806-4760 0103-6440 http://hdl.handle.net/11449/189288 10.1590/0103-6440201902280 S0103-64402019000300213 2-s2.0-85067516519 S0103-64402019000300213.pdf |
url |
http://dx.doi.org/10.1590/0103-6440201902280 http://hdl.handle.net/11449/189288 |
identifier_str_mv |
Brazilian Dental Journal, v. 30, n. 3, p. 213-219, 2019. 1806-4760 0103-6440 10.1590/0103-6440201902280 S0103-64402019000300213 2-s2.0-85067516519 S0103-64402019000300213.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Brazilian Dental Journal |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
213-219 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1799965157298798592 |