Titanium-enriched medium drives low profile of ECM remodeling as a pre-requisite to pre-osteoblast viability and proliferative phenotype
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.jtemb.2018.07.015 http://hdl.handle.net/11449/176666 |
Resumo: | Titanium is widely used for biomedical applications, but little information is being delivered regarding the cellular/molecular mechanisms explaining their efficacy, mainly considering the effects of the Ti-released trace elements on pre-osteoblasts. We addressed this issue by investigating decisive intracellular signal transduction able to modulate cytoskeleton rearrangement, proliferative phenotype and extracellular matrix (ECM) remodeling. We considered titanium grades IV and V, submitted or not to dual acid-etching (w/DAE or wo/DAE, respectively). Our results showed there is no cytotoxicity, preserving AKT involvement. Additionally, Ti-enriched medium promoted a diminution of the downstream signaling upon integrin activation (phosphorylating Rac1 and cofilin), guaranteeing a dynamic cytoskeleton rearrangement. Moreover, the low profile of ECM remodeling obtained in response to trace molecules released by Ti-based devices seems contributing to the osteoblast performance in mediating extracellular support to cell anchorage. This hypothesis was validated by the up-expression of ß1-integrin, src and Focal adhesion kinase (fak) genes, mainly in response to titanium grade V. Proliferative phenotype showed an unbalance between cyclin-dependent kinases (CDKs) and p15INK4b/p21Cip1. In conjunction, we showed for the first time that trace elements from Ti-based biomedical devices provoke important modulation of the osteoblast biology, driving cell anchoring, viability, and proliferative phenotype. Certainly, these biological outcomes compromise implant osseointegration. |
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Titanium-enriched medium drives low profile of ECM remodeling as a pre-requisite to pre-osteoblast viability and proliferative phenotypeCell adhesionCell signallingExtracellular matrixPre-osteoblastTitaniumTitanium is widely used for biomedical applications, but little information is being delivered regarding the cellular/molecular mechanisms explaining their efficacy, mainly considering the effects of the Ti-released trace elements on pre-osteoblasts. We addressed this issue by investigating decisive intracellular signal transduction able to modulate cytoskeleton rearrangement, proliferative phenotype and extracellular matrix (ECM) remodeling. We considered titanium grades IV and V, submitted or not to dual acid-etching (w/DAE or wo/DAE, respectively). Our results showed there is no cytotoxicity, preserving AKT involvement. Additionally, Ti-enriched medium promoted a diminution of the downstream signaling upon integrin activation (phosphorylating Rac1 and cofilin), guaranteeing a dynamic cytoskeleton rearrangement. Moreover, the low profile of ECM remodeling obtained in response to trace molecules released by Ti-based devices seems contributing to the osteoblast performance in mediating extracellular support to cell anchorage. This hypothesis was validated by the up-expression of ß1-integrin, src and Focal adhesion kinase (fak) genes, mainly in response to titanium grade V. Proliferative phenotype showed an unbalance between cyclin-dependent kinases (CDKs) and p15INK4b/p21Cip1. In conjunction, we showed for the first time that trace elements from Ti-based biomedical devices provoke important modulation of the osteoblast biology, driving cell anchoring, viability, and proliferative phenotype. Certainly, these biological outcomes compromise implant osseointegration.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Dept. of Chemistry and Biochemistry Bioscience Institute São Paulo State University UNESP Campus BotucatuElectron Microscopy Center IBB UNESPDept. of Chemistry and Biochemistry Bioscience Institute São Paulo State University UNESP Campus BotucatuElectron Microscopy Center IBB UNESPCNPq: # 477452/2012-4FAPESP: #2014/22689-3CNPq: #301966/2015-0Universidade Estadual Paulista (Unesp)da Costa Fernandes, Celio J. [UNESP]Bezerra, Fábio J.B. [UNESP]de Campos Souza, Bruno [UNESP]Campos, Mônica Aparecida [UNESP]Zambuzzi, Willian Fernando [UNESP]2018-12-11T17:21:59Z2018-12-11T17:21:59Z2018-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article339-346application/pdfhttp://dx.doi.org/10.1016/j.jtemb.2018.07.015Journal of Trace Elements in Medicine and Biology, v. 50, p. 339-346.1878-32520946-672Xhttp://hdl.handle.net/11449/17666610.1016/j.jtemb.2018.07.0152-s2.0-850508909792-s2.0-85050890979.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Trace Elements in Medicine and Biologyinfo:eu-repo/semantics/openAccess2024-01-05T06:27:16Zoai:repositorio.unesp.br:11449/176666Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-01-05T06:27:16Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Titanium-enriched medium drives low profile of ECM remodeling as a pre-requisite to pre-osteoblast viability and proliferative phenotype |
title |
Titanium-enriched medium drives low profile of ECM remodeling as a pre-requisite to pre-osteoblast viability and proliferative phenotype |
spellingShingle |
Titanium-enriched medium drives low profile of ECM remodeling as a pre-requisite to pre-osteoblast viability and proliferative phenotype da Costa Fernandes, Celio J. [UNESP] Cell adhesion Cell signalling Extracellular matrix Pre-osteoblast Titanium |
title_short |
Titanium-enriched medium drives low profile of ECM remodeling as a pre-requisite to pre-osteoblast viability and proliferative phenotype |
title_full |
Titanium-enriched medium drives low profile of ECM remodeling as a pre-requisite to pre-osteoblast viability and proliferative phenotype |
title_fullStr |
Titanium-enriched medium drives low profile of ECM remodeling as a pre-requisite to pre-osteoblast viability and proliferative phenotype |
title_full_unstemmed |
Titanium-enriched medium drives low profile of ECM remodeling as a pre-requisite to pre-osteoblast viability and proliferative phenotype |
title_sort |
Titanium-enriched medium drives low profile of ECM remodeling as a pre-requisite to pre-osteoblast viability and proliferative phenotype |
author |
da Costa Fernandes, Celio J. [UNESP] |
author_facet |
da Costa Fernandes, Celio J. [UNESP] Bezerra, Fábio J.B. [UNESP] de Campos Souza, Bruno [UNESP] Campos, Mônica Aparecida [UNESP] Zambuzzi, Willian Fernando [UNESP] |
author_role |
author |
author2 |
Bezerra, Fábio J.B. [UNESP] de Campos Souza, Bruno [UNESP] Campos, Mônica Aparecida [UNESP] Zambuzzi, Willian Fernando [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
da Costa Fernandes, Celio J. [UNESP] Bezerra, Fábio J.B. [UNESP] de Campos Souza, Bruno [UNESP] Campos, Mônica Aparecida [UNESP] Zambuzzi, Willian Fernando [UNESP] |
dc.subject.por.fl_str_mv |
Cell adhesion Cell signalling Extracellular matrix Pre-osteoblast Titanium |
topic |
Cell adhesion Cell signalling Extracellular matrix Pre-osteoblast Titanium |
description |
Titanium is widely used for biomedical applications, but little information is being delivered regarding the cellular/molecular mechanisms explaining their efficacy, mainly considering the effects of the Ti-released trace elements on pre-osteoblasts. We addressed this issue by investigating decisive intracellular signal transduction able to modulate cytoskeleton rearrangement, proliferative phenotype and extracellular matrix (ECM) remodeling. We considered titanium grades IV and V, submitted or not to dual acid-etching (w/DAE or wo/DAE, respectively). Our results showed there is no cytotoxicity, preserving AKT involvement. Additionally, Ti-enriched medium promoted a diminution of the downstream signaling upon integrin activation (phosphorylating Rac1 and cofilin), guaranteeing a dynamic cytoskeleton rearrangement. Moreover, the low profile of ECM remodeling obtained in response to trace molecules released by Ti-based devices seems contributing to the osteoblast performance in mediating extracellular support to cell anchorage. This hypothesis was validated by the up-expression of ß1-integrin, src and Focal adhesion kinase (fak) genes, mainly in response to titanium grade V. Proliferative phenotype showed an unbalance between cyclin-dependent kinases (CDKs) and p15INK4b/p21Cip1. In conjunction, we showed for the first time that trace elements from Ti-based biomedical devices provoke important modulation of the osteoblast biology, driving cell anchoring, viability, and proliferative phenotype. Certainly, these biological outcomes compromise implant osseointegration. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-12-11T17:21:59Z 2018-12-11T17:21:59Z 2018-12-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.jtemb.2018.07.015 Journal of Trace Elements in Medicine and Biology, v. 50, p. 339-346. 1878-3252 0946-672X http://hdl.handle.net/11449/176666 10.1016/j.jtemb.2018.07.015 2-s2.0-85050890979 2-s2.0-85050890979.pdf |
url |
http://dx.doi.org/10.1016/j.jtemb.2018.07.015 http://hdl.handle.net/11449/176666 |
identifier_str_mv |
Journal of Trace Elements in Medicine and Biology, v. 50, p. 339-346. 1878-3252 0946-672X 10.1016/j.jtemb.2018.07.015 2-s2.0-85050890979 2-s2.0-85050890979.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Trace Elements in Medicine and Biology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
339-346 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965527074930688 |