Effect of different pre-irradiation times on curcumin-mediated photodynamic therapy against planktonic cultures and biofilms of Candida spp
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.archoralbio.2012.10.011 http://hdl.handle.net/11449/74465 |
Resumo: | Objectives: The aim of this study was to evaluate the effects of pre-irradiation time (PIT) on curcumin (Cur)-mediated photodynamic therapy (PDT) against planktonic and biofilm cultures of reference strains of Candida albicans, Candida glabrata and Candida dubliniensis. Materials and methods: Suspensions and biofilms of Candida species were maintained in contact with different concentrations of Cur for time intervals of 1, 5, 10 and 20 min before irradiation and LED (light emitting diode) activation. Additional samples were treated only with Cur, without illumination, or only with light, without Cur. Control samples received neither light nor Cur. After PDT, suspensions were plated on Sabouraud Dextrose Agar, while biofilm results were obtained using the XTT-salt reduction method. Confocal Laser Scanning Microscopy (CLSM) observations were performed to supply a better understanding of Cur penetration through the biofilms after 5 and 20 min of contact with the cultures. Results: Different PITs showed no statistical differences in Cur-mediated PDT of Candida spp. cell suspensions. There was complete inactivation of the three Candida species with the association of 20.0 μM Cur after 5, 10 and 20 min of PIT. Biofilm cultures showed significant reduction in cell viability after PDT. In general, the three Candida species evaluated in this study suffered higher reductions in cell viability with the association of 40.0 μM Cur and 20 min of PIT. Additionally, CLSM observations showed different intensities of fluorescence emissions after 5 and 20 min of incubation. Conclusion: Photoinactivation of planktonic cultures was not PIT-dependent. PIT-dependence of the biofilm cultures differed among the species evaluated. Also, CLSM observations confirmed the need of higher time intervals for the Cur to penetrate biofilm structures. © 2012 Elsevier Ltd. All rights reserved. |
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Effect of different pre-irradiation times on curcumin-mediated photodynamic therapy against planktonic cultures and biofilms of Candida sppBiofilmsCandidaCurcuminDrug resistancePhotochemotherapyCandida albicansCandida dubliniensisCandida glabrataObjectives: The aim of this study was to evaluate the effects of pre-irradiation time (PIT) on curcumin (Cur)-mediated photodynamic therapy (PDT) against planktonic and biofilm cultures of reference strains of Candida albicans, Candida glabrata and Candida dubliniensis. Materials and methods: Suspensions and biofilms of Candida species were maintained in contact with different concentrations of Cur for time intervals of 1, 5, 10 and 20 min before irradiation and LED (light emitting diode) activation. Additional samples were treated only with Cur, without illumination, or only with light, without Cur. Control samples received neither light nor Cur. After PDT, suspensions were plated on Sabouraud Dextrose Agar, while biofilm results were obtained using the XTT-salt reduction method. Confocal Laser Scanning Microscopy (CLSM) observations were performed to supply a better understanding of Cur penetration through the biofilms after 5 and 20 min of contact with the cultures. Results: Different PITs showed no statistical differences in Cur-mediated PDT of Candida spp. cell suspensions. There was complete inactivation of the three Candida species with the association of 20.0 μM Cur after 5, 10 and 20 min of PIT. Biofilm cultures showed significant reduction in cell viability after PDT. In general, the three Candida species evaluated in this study suffered higher reductions in cell viability with the association of 40.0 μM Cur and 20 min of PIT. Additionally, CLSM observations showed different intensities of fluorescence emissions after 5 and 20 min of incubation. Conclusion: Photoinactivation of planktonic cultures was not PIT-dependent. PIT-dependence of the biofilm cultures differed among the species evaluated. Also, CLSM observations confirmed the need of higher time intervals for the Cur to penetrate biofilm structures. © 2012 Elsevier Ltd. All rights reserved.Department of Dental Materials and Prosthodontics Araraquara Dental School Universidade Estadual Paulista (UNESP), Araraquara, SPDepartment of Operative Dentistry University of Brasília - UnB, Brasília, DFDepartment of Social Dentistry Araraquara Dental School Universidade Estadual Paulista (UNESP), Araraquara, Araraquara, SPDepartment of Clinical Analysis Universidade Estadual Paulista (UNESP), Araraquara, SPPhysics Institute of São Carlos University of São Paulo - USP, São Carlos, SPFaculdade de Odontologia de Araraquara Universidade Estadual Paulista/UNESP, Rua Humaitá, 1680, Centro, CEP: 14801-903 Araraquara, SPDepartment of Dental Materials and Prosthodontics Araraquara Dental School Universidade Estadual Paulista (UNESP), Araraquara, SPDepartment of Social Dentistry Araraquara Dental School Universidade Estadual Paulista (UNESP), Araraquara, Araraquara, SPDepartment of Clinical Analysis Universidade Estadual Paulista (UNESP), Araraquara, SPFaculdade de Odontologia de Araraquara Universidade Estadual Paulista/UNESP, Rua Humaitá, 1680, Centro, CEP: 14801-903 Araraquara, SPUniversidade Estadual Paulista (Unesp)Universidade de Brasília (UnB)Universidade de São Paulo (USP)Andrade, Mariana Carvalho [UNESP]Ribeiro, Ana Paula DiasDovigo, Lívia Nordi [UNESP]Brunetti, Iguatemy Lourenço [UNESP]Giampaolo, Eunice Teresinha [UNESP]Bagnato, Vanderlei SalvadorPavarina, Ana Claudia [UNESP]2014-05-27T11:28:17Z2014-05-27T11:28:17Z2013-02-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article200-210application/pdfhttp://dx.doi.org/10.1016/j.archoralbio.2012.10.011Archives of Oral Biology, v. 58, n. 2, p. 200-210, 2013.0003-9969http://hdl.handle.net/11449/7446510.1016/j.archoralbio.2012.10.011WOS:0003141996000112-s2.0-848741167562-s2.0-84874116756.pdf573712733424854988676705391054030127570480681342Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengArchives of Oral Biology2.0500,752info:eu-repo/semantics/openAccess2023-11-14T06:14:46Zoai:repositorio.unesp.br:11449/74465Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-11-14T06:14:46Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Effect of different pre-irradiation times on curcumin-mediated photodynamic therapy against planktonic cultures and biofilms of Candida spp |
title |
Effect of different pre-irradiation times on curcumin-mediated photodynamic therapy against planktonic cultures and biofilms of Candida spp |
spellingShingle |
Effect of different pre-irradiation times on curcumin-mediated photodynamic therapy against planktonic cultures and biofilms of Candida spp Andrade, Mariana Carvalho [UNESP] Biofilms Candida Curcumin Drug resistance Photochemotherapy Candida albicans Candida dubliniensis Candida glabrata |
title_short |
Effect of different pre-irradiation times on curcumin-mediated photodynamic therapy against planktonic cultures and biofilms of Candida spp |
title_full |
Effect of different pre-irradiation times on curcumin-mediated photodynamic therapy against planktonic cultures and biofilms of Candida spp |
title_fullStr |
Effect of different pre-irradiation times on curcumin-mediated photodynamic therapy against planktonic cultures and biofilms of Candida spp |
title_full_unstemmed |
Effect of different pre-irradiation times on curcumin-mediated photodynamic therapy against planktonic cultures and biofilms of Candida spp |
title_sort |
Effect of different pre-irradiation times on curcumin-mediated photodynamic therapy against planktonic cultures and biofilms of Candida spp |
author |
Andrade, Mariana Carvalho [UNESP] |
author_facet |
Andrade, Mariana Carvalho [UNESP] Ribeiro, Ana Paula Dias Dovigo, Lívia Nordi [UNESP] Brunetti, Iguatemy Lourenço [UNESP] Giampaolo, Eunice Teresinha [UNESP] Bagnato, Vanderlei Salvador Pavarina, Ana Claudia [UNESP] |
author_role |
author |
author2 |
Ribeiro, Ana Paula Dias Dovigo, Lívia Nordi [UNESP] Brunetti, Iguatemy Lourenço [UNESP] Giampaolo, Eunice Teresinha [UNESP] Bagnato, Vanderlei Salvador Pavarina, Ana Claudia [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade de Brasília (UnB) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Andrade, Mariana Carvalho [UNESP] Ribeiro, Ana Paula Dias Dovigo, Lívia Nordi [UNESP] Brunetti, Iguatemy Lourenço [UNESP] Giampaolo, Eunice Teresinha [UNESP] Bagnato, Vanderlei Salvador Pavarina, Ana Claudia [UNESP] |
dc.subject.por.fl_str_mv |
Biofilms Candida Curcumin Drug resistance Photochemotherapy Candida albicans Candida dubliniensis Candida glabrata |
topic |
Biofilms Candida Curcumin Drug resistance Photochemotherapy Candida albicans Candida dubliniensis Candida glabrata |
description |
Objectives: The aim of this study was to evaluate the effects of pre-irradiation time (PIT) on curcumin (Cur)-mediated photodynamic therapy (PDT) against planktonic and biofilm cultures of reference strains of Candida albicans, Candida glabrata and Candida dubliniensis. Materials and methods: Suspensions and biofilms of Candida species were maintained in contact with different concentrations of Cur for time intervals of 1, 5, 10 and 20 min before irradiation and LED (light emitting diode) activation. Additional samples were treated only with Cur, without illumination, or only with light, without Cur. Control samples received neither light nor Cur. After PDT, suspensions were plated on Sabouraud Dextrose Agar, while biofilm results were obtained using the XTT-salt reduction method. Confocal Laser Scanning Microscopy (CLSM) observations were performed to supply a better understanding of Cur penetration through the biofilms after 5 and 20 min of contact with the cultures. Results: Different PITs showed no statistical differences in Cur-mediated PDT of Candida spp. cell suspensions. There was complete inactivation of the three Candida species with the association of 20.0 μM Cur after 5, 10 and 20 min of PIT. Biofilm cultures showed significant reduction in cell viability after PDT. In general, the three Candida species evaluated in this study suffered higher reductions in cell viability with the association of 40.0 μM Cur and 20 min of PIT. Additionally, CLSM observations showed different intensities of fluorescence emissions after 5 and 20 min of incubation. Conclusion: Photoinactivation of planktonic cultures was not PIT-dependent. PIT-dependence of the biofilm cultures differed among the species evaluated. Also, CLSM observations confirmed the need of higher time intervals for the Cur to penetrate biofilm structures. © 2012 Elsevier Ltd. All rights reserved. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-02-01 2014-05-27T11:28:17Z 2014-05-27T11:28:17Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.archoralbio.2012.10.011 Archives of Oral Biology, v. 58, n. 2, p. 200-210, 2013. 0003-9969 http://hdl.handle.net/11449/74465 10.1016/j.archoralbio.2012.10.011 WOS:000314199600011 2-s2.0-84874116756 2-s2.0-84874116756.pdf 5737127334248549 8867670539105403 0127570480681342 |
url |
http://dx.doi.org/10.1016/j.archoralbio.2012.10.011 http://hdl.handle.net/11449/74465 |
identifier_str_mv |
Archives of Oral Biology, v. 58, n. 2, p. 200-210, 2013. 0003-9969 10.1016/j.archoralbio.2012.10.011 WOS:000314199600011 2-s2.0-84874116756 2-s2.0-84874116756.pdf 5737127334248549 8867670539105403 0127570480681342 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Archives of Oral Biology 2.050 0,752 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
200-210 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1797789706364125184 |