Zn:DLC films via PECVD-HIPIMS: Evaluation of antimicrobial activity and cytotoxicity to mammalian cells
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1116/6.0002354 http://hdl.handle.net/11449/249795 |
Resumo: | DLC films were grown with Zn via a combined plasma-enhanced chemical vapor deposition (PECVD) and high-power impulse magnetron sputtering (HIPIMS) process. The films were deposited on textiles in an atmosphere of Ar and C2H2, and the percentage of metal in the DLC was varied by controlling the acetylene gas flow. At first, to evaluate the antimicrobial activity, a screening test with the ISO 22196 standard was carried out. Afterward, AATCC TM100:2019 was used to evaluate the antimicrobial effectiveness of the films on textiles. The antimicrobial effectiveness of the coating was studied against a Gram-negative bacterium (Escherichia coli), a Gram-positive bacterium (Staphylococcus aureus), and a fungus (Candida albicans), after a 24 h contact. In addition, the cytotoxicity of the samples to mammalian cells was evaluated by indirect contact. For this, the samples were soaked into the growth media for 1 and 7 days, and then, the extracts were collected and put in contact with keratinocytes for 24 h. Finally, the properties of the films were also evaluated as a function of the Zn content, such as their structural quality, morphology, hardness, wear resistance, and coefficient of friction. The films showed excellent results against all microorganisms, with 100% effectiveness in some cases. The pure extracts obtained from all the samples with the incorporation of metals were cytotoxic. Despite that, the cell viability after contact with some Zn-DLC diluted extracts (10%) was not different from that observed in the uncoated group. Besides, increasing the Zn content resulted in a film with poorer mechanical properties but did not affect the coefficient of friction of the coating. |
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Zn:DLC films via PECVD-HIPIMS: Evaluation of antimicrobial activity and cytotoxicity to mammalian cellsDLC films were grown with Zn via a combined plasma-enhanced chemical vapor deposition (PECVD) and high-power impulse magnetron sputtering (HIPIMS) process. The films were deposited on textiles in an atmosphere of Ar and C2H2, and the percentage of metal in the DLC was varied by controlling the acetylene gas flow. At first, to evaluate the antimicrobial activity, a screening test with the ISO 22196 standard was carried out. Afterward, AATCC TM100:2019 was used to evaluate the antimicrobial effectiveness of the films on textiles. The antimicrobial effectiveness of the coating was studied against a Gram-negative bacterium (Escherichia coli), a Gram-positive bacterium (Staphylococcus aureus), and a fungus (Candida albicans), after a 24 h contact. In addition, the cytotoxicity of the samples to mammalian cells was evaluated by indirect contact. For this, the samples were soaked into the growth media for 1 and 7 days, and then, the extracts were collected and put in contact with keratinocytes for 24 h. Finally, the properties of the films were also evaluated as a function of the Zn content, such as their structural quality, morphology, hardness, wear resistance, and coefficient of friction. The films showed excellent results against all microorganisms, with 100% effectiveness in some cases. The pure extracts obtained from all the samples with the incorporation of metals were cytotoxic. Despite that, the cell viability after contact with some Zn-DLC diluted extracts (10%) was not different from that observed in the uncoated group. Besides, increasing the Zn content resulted in a film with poorer mechanical properties but did not affect the coefficient of friction of the coating.National Institute for Space Research - INPE, Astronautas Avenue, 1.758 - Jardim da Granja, São José dos CamposFederal University of São Paulo - UNIFESP, Talim Street, 330 - Vila Nair, São José dos CamposFraunhofer Institute for Surface Engineering and Thin Films (IST), Bienroder Weg 54EInstitute of Science and Technology São Paulo State University (Unesp), Eng. Francisco José Longo Avenue, 777 - Jardim São Dimas, São José dos CamposInstitute of Science and Technology São Paulo State University (Unesp), Eng. Francisco José Longo Avenue, 777 - Jardim São Dimas, São José dos CamposNational Institute for Space Research - INPEUniversidade de São Paulo (USP)Fraunhofer Institute for Surface Engineering and Thin Films (IST)Universidade Estadual Paulista (UNESP)De O. Correia, Rebeca F.B.Sampaio, Aline G. [UNESP]Milhan, Noala V.M. [UNESP]Capote, ArielGerdes, HolgerLachmann, KristinaTrava-Airoldi, Vladimir J.Koga-Ito, Cristiane Yumi [UNESP]Bandorf, Ralf2023-07-29T16:09:26Z2023-07-29T16:09:26Z2023-05-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1116/6.0002354Journal of Vacuum Science and Technology A: Vacuum, Surfaces and Films, v. 41, n. 3, 2023.1520-85590734-2101http://hdl.handle.net/11449/24979510.1116/6.00023542-s2.0-85150906626Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Vacuum Science and Technology A: Vacuum, Surfaces and Filmsinfo:eu-repo/semantics/openAccess2023-07-29T16:09:27Zoai:repositorio.unesp.br:11449/249795Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-07-29T16:09:27Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Zn:DLC films via PECVD-HIPIMS: Evaluation of antimicrobial activity and cytotoxicity to mammalian cells |
title |
Zn:DLC films via PECVD-HIPIMS: Evaluation of antimicrobial activity and cytotoxicity to mammalian cells |
spellingShingle |
Zn:DLC films via PECVD-HIPIMS: Evaluation of antimicrobial activity and cytotoxicity to mammalian cells De O. Correia, Rebeca F.B. |
title_short |
Zn:DLC films via PECVD-HIPIMS: Evaluation of antimicrobial activity and cytotoxicity to mammalian cells |
title_full |
Zn:DLC films via PECVD-HIPIMS: Evaluation of antimicrobial activity and cytotoxicity to mammalian cells |
title_fullStr |
Zn:DLC films via PECVD-HIPIMS: Evaluation of antimicrobial activity and cytotoxicity to mammalian cells |
title_full_unstemmed |
Zn:DLC films via PECVD-HIPIMS: Evaluation of antimicrobial activity and cytotoxicity to mammalian cells |
title_sort |
Zn:DLC films via PECVD-HIPIMS: Evaluation of antimicrobial activity and cytotoxicity to mammalian cells |
author |
De O. Correia, Rebeca F.B. |
author_facet |
De O. Correia, Rebeca F.B. Sampaio, Aline G. [UNESP] Milhan, Noala V.M. [UNESP] Capote, Ariel Gerdes, Holger Lachmann, Kristina Trava-Airoldi, Vladimir J. Koga-Ito, Cristiane Yumi [UNESP] Bandorf, Ralf |
author_role |
author |
author2 |
Sampaio, Aline G. [UNESP] Milhan, Noala V.M. [UNESP] Capote, Ariel Gerdes, Holger Lachmann, Kristina Trava-Airoldi, Vladimir J. Koga-Ito, Cristiane Yumi [UNESP] Bandorf, Ralf |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
National Institute for Space Research - INPE Universidade de São Paulo (USP) Fraunhofer Institute for Surface Engineering and Thin Films (IST) Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
De O. Correia, Rebeca F.B. Sampaio, Aline G. [UNESP] Milhan, Noala V.M. [UNESP] Capote, Ariel Gerdes, Holger Lachmann, Kristina Trava-Airoldi, Vladimir J. Koga-Ito, Cristiane Yumi [UNESP] Bandorf, Ralf |
description |
DLC films were grown with Zn via a combined plasma-enhanced chemical vapor deposition (PECVD) and high-power impulse magnetron sputtering (HIPIMS) process. The films were deposited on textiles in an atmosphere of Ar and C2H2, and the percentage of metal in the DLC was varied by controlling the acetylene gas flow. At first, to evaluate the antimicrobial activity, a screening test with the ISO 22196 standard was carried out. Afterward, AATCC TM100:2019 was used to evaluate the antimicrobial effectiveness of the films on textiles. The antimicrobial effectiveness of the coating was studied against a Gram-negative bacterium (Escherichia coli), a Gram-positive bacterium (Staphylococcus aureus), and a fungus (Candida albicans), after a 24 h contact. In addition, the cytotoxicity of the samples to mammalian cells was evaluated by indirect contact. For this, the samples were soaked into the growth media for 1 and 7 days, and then, the extracts were collected and put in contact with keratinocytes for 24 h. Finally, the properties of the films were also evaluated as a function of the Zn content, such as their structural quality, morphology, hardness, wear resistance, and coefficient of friction. The films showed excellent results against all microorganisms, with 100% effectiveness in some cases. The pure extracts obtained from all the samples with the incorporation of metals were cytotoxic. Despite that, the cell viability after contact with some Zn-DLC diluted extracts (10%) was not different from that observed in the uncoated group. Besides, increasing the Zn content resulted in a film with poorer mechanical properties but did not affect the coefficient of friction of the coating. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-07-29T16:09:26Z 2023-07-29T16:09:26Z 2023-05-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1116/6.0002354 Journal of Vacuum Science and Technology A: Vacuum, Surfaces and Films, v. 41, n. 3, 2023. 1520-8559 0734-2101 http://hdl.handle.net/11449/249795 10.1116/6.0002354 2-s2.0-85150906626 |
url |
http://dx.doi.org/10.1116/6.0002354 http://hdl.handle.net/11449/249795 |
identifier_str_mv |
Journal of Vacuum Science and Technology A: Vacuum, Surfaces and Films, v. 41, n. 3, 2023. 1520-8559 0734-2101 10.1116/6.0002354 2-s2.0-85150906626 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Vacuum Science and Technology A: Vacuum, Surfaces and Films |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1799965198922022912 |