Genetic diversity of the pampas deer (Ozotoceros bezoarticus) population in the Brazilian pantanal assessed by combining fresh fecal DNA analysis and a set of heterologous microsatellite loci

Detalhes bibliográficos
Autor(a) principal: Mantellatto, Aline Meira Bonfim [UNESP]
Data de Publicação: 2017
Outros Autores: Caparroz, Renato, Christofoletti, Maurício Durante [UNESP], Piovezan, Ubiratan, Duarte, José Maurício Barbanti [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/1678-4685-gmb-2016-0323
http://hdl.handle.net/11449/179401
Resumo: The pampas deer (Ozotoceros bezoarticus) is close to being classified as globally threatened’, with the largest population occurring in the Brazilian Pantanal. Since capture is stressful to these animals, non-invasive sampling methods such as the use of feces can provide reliable sources of DNA. The aim of this study was to use fecal samples to evaluate the genetic variability of the Brazilian Pantanal population of pampas deer. Six heterologous microsatellite markers were used to screen 142 stool specimens. Seventy-four deer were identified, of which 50 adults were used to determine the genetic characteristics of the population. The Pantanal population showed high genetic diversity (mean number of alleles per locus = 11.5, expected heterozygosity = 0.75). This is the first investigation to characterize a South American deer species using fecal DNA and demonstrates the usefulness and efficiency of this approach, as well as the feasibility of obtaining information that could not have been easily obtained by traditional DNA sampling. Our findings suggest that management strategies for this species may be much more effective if applied now when the population still shows high genetic variability.
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spelling Genetic diversity of the pampas deer (Ozotoceros bezoarticus) population in the Brazilian pantanal assessed by combining fresh fecal DNA analysis and a set of heterologous microsatellite lociConservationFecal DNAMicrosatellitesNon-invasive methodsPampas deerThe pampas deer (Ozotoceros bezoarticus) is close to being classified as globally threatened’, with the largest population occurring in the Brazilian Pantanal. Since capture is stressful to these animals, non-invasive sampling methods such as the use of feces can provide reliable sources of DNA. The aim of this study was to use fecal samples to evaluate the genetic variability of the Brazilian Pantanal population of pampas deer. Six heterologous microsatellite markers were used to screen 142 stool specimens. Seventy-four deer were identified, of which 50 adults were used to determine the genetic characteristics of the population. The Pantanal population showed high genetic diversity (mean number of alleles per locus = 11.5, expected heterozygosity = 0.75). This is the first investigation to characterize a South American deer species using fecal DNA and demonstrates the usefulness and efficiency of this approach, as well as the feasibility of obtaining information that could not have been easily obtained by traditional DNA sampling. Our findings suggest that management strategies for this species may be much more effective if applied now when the population still shows high genetic variability.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Núcleo de Pesquisa e Conservação de Cervídeos Departamento de Zootecnia Universidade Estadual Paulista “Júlio de Mesquita Filho” (UNESP)Departamento de Genética e Morfologia Instituto de Ciências Biológicas Universidade de BrasíliaCentro de Pesquisas Agropecuárias do Pantanal (CPAP), EmbrapaNúcleo de Pesquisa e Conservação de Cervídeos Departamento de Zootecnia Universidade Estadual Paulista “Júlio de Mesquita Filho” (UNESP)FAPESP: 2009/04253-5Universidade Estadual Paulista (Unesp)Universidade de Brasília (UnB)Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)Mantellatto, Aline Meira Bonfim [UNESP]Caparroz, RenatoChristofoletti, Maurício Durante [UNESP]Piovezan, UbiratanDuarte, José Maurício Barbanti [UNESP]2018-12-11T17:35:02Z2018-12-11T17:35:02Z2017-10-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article774-780application/pdfhttp://dx.doi.org/10.1590/1678-4685-gmb-2016-0323Genetics and Molecular Biology, v. 40, n. 4, p. 774-780, 2017.1678-46851415-4757http://hdl.handle.net/11449/17940110.1590/1678-4685-gmb-2016-0323S1415-475720170005007742-s2.0-85035795479S1415-47572017000500774.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengGenetics and Molecular Biology0,638info:eu-repo/semantics/openAccess2023-12-26T06:22:29Zoai:repositorio.unesp.br:11449/179401Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-12-26T06:22:29Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Genetic diversity of the pampas deer (Ozotoceros bezoarticus) population in the Brazilian pantanal assessed by combining fresh fecal DNA analysis and a set of heterologous microsatellite loci
title Genetic diversity of the pampas deer (Ozotoceros bezoarticus) population in the Brazilian pantanal assessed by combining fresh fecal DNA analysis and a set of heterologous microsatellite loci
spellingShingle Genetic diversity of the pampas deer (Ozotoceros bezoarticus) population in the Brazilian pantanal assessed by combining fresh fecal DNA analysis and a set of heterologous microsatellite loci
Mantellatto, Aline Meira Bonfim [UNESP]
Conservation
Fecal DNA
Microsatellites
Non-invasive methods
Pampas deer
title_short Genetic diversity of the pampas deer (Ozotoceros bezoarticus) population in the Brazilian pantanal assessed by combining fresh fecal DNA analysis and a set of heterologous microsatellite loci
title_full Genetic diversity of the pampas deer (Ozotoceros bezoarticus) population in the Brazilian pantanal assessed by combining fresh fecal DNA analysis and a set of heterologous microsatellite loci
title_fullStr Genetic diversity of the pampas deer (Ozotoceros bezoarticus) population in the Brazilian pantanal assessed by combining fresh fecal DNA analysis and a set of heterologous microsatellite loci
title_full_unstemmed Genetic diversity of the pampas deer (Ozotoceros bezoarticus) population in the Brazilian pantanal assessed by combining fresh fecal DNA analysis and a set of heterologous microsatellite loci
title_sort Genetic diversity of the pampas deer (Ozotoceros bezoarticus) population in the Brazilian pantanal assessed by combining fresh fecal DNA analysis and a set of heterologous microsatellite loci
author Mantellatto, Aline Meira Bonfim [UNESP]
author_facet Mantellatto, Aline Meira Bonfim [UNESP]
Caparroz, Renato
Christofoletti, Maurício Durante [UNESP]
Piovezan, Ubiratan
Duarte, José Maurício Barbanti [UNESP]
author_role author
author2 Caparroz, Renato
Christofoletti, Maurício Durante [UNESP]
Piovezan, Ubiratan
Duarte, José Maurício Barbanti [UNESP]
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Universidade de Brasília (UnB)
Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)
dc.contributor.author.fl_str_mv Mantellatto, Aline Meira Bonfim [UNESP]
Caparroz, Renato
Christofoletti, Maurício Durante [UNESP]
Piovezan, Ubiratan
Duarte, José Maurício Barbanti [UNESP]
dc.subject.por.fl_str_mv Conservation
Fecal DNA
Microsatellites
Non-invasive methods
Pampas deer
topic Conservation
Fecal DNA
Microsatellites
Non-invasive methods
Pampas deer
description The pampas deer (Ozotoceros bezoarticus) is close to being classified as globally threatened’, with the largest population occurring in the Brazilian Pantanal. Since capture is stressful to these animals, non-invasive sampling methods such as the use of feces can provide reliable sources of DNA. The aim of this study was to use fecal samples to evaluate the genetic variability of the Brazilian Pantanal population of pampas deer. Six heterologous microsatellite markers were used to screen 142 stool specimens. Seventy-four deer were identified, of which 50 adults were used to determine the genetic characteristics of the population. The Pantanal population showed high genetic diversity (mean number of alleles per locus = 11.5, expected heterozygosity = 0.75). This is the first investigation to characterize a South American deer species using fecal DNA and demonstrates the usefulness and efficiency of this approach, as well as the feasibility of obtaining information that could not have been easily obtained by traditional DNA sampling. Our findings suggest that management strategies for this species may be much more effective if applied now when the population still shows high genetic variability.
publishDate 2017
dc.date.none.fl_str_mv 2017-10-01
2018-12-11T17:35:02Z
2018-12-11T17:35:02Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/1678-4685-gmb-2016-0323
Genetics and Molecular Biology, v. 40, n. 4, p. 774-780, 2017.
1678-4685
1415-4757
http://hdl.handle.net/11449/179401
10.1590/1678-4685-gmb-2016-0323
S1415-47572017000500774
2-s2.0-85035795479
S1415-47572017000500774.pdf
url http://dx.doi.org/10.1590/1678-4685-gmb-2016-0323
http://hdl.handle.net/11449/179401
identifier_str_mv Genetics and Molecular Biology, v. 40, n. 4, p. 774-780, 2017.
1678-4685
1415-4757
10.1590/1678-4685-gmb-2016-0323
S1415-47572017000500774
2-s2.0-85035795479
S1415-47572017000500774.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Genetics and Molecular Biology
0,638
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 774-780
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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