Genetic diversity of the pampas deer (Ozotoceros bezoarticus) population in the Brazilian pantanal assessed by combining fresh fecal DNA analysis and a set of heterologous microsatellite loci
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/1678-4685-gmb-2016-0323 http://hdl.handle.net/11449/179401 |
Resumo: | The pampas deer (Ozotoceros bezoarticus) is close to being classified as globally threatened’, with the largest population occurring in the Brazilian Pantanal. Since capture is stressful to these animals, non-invasive sampling methods such as the use of feces can provide reliable sources of DNA. The aim of this study was to use fecal samples to evaluate the genetic variability of the Brazilian Pantanal population of pampas deer. Six heterologous microsatellite markers were used to screen 142 stool specimens. Seventy-four deer were identified, of which 50 adults were used to determine the genetic characteristics of the population. The Pantanal population showed high genetic diversity (mean number of alleles per locus = 11.5, expected heterozygosity = 0.75). This is the first investigation to characterize a South American deer species using fecal DNA and demonstrates the usefulness and efficiency of this approach, as well as the feasibility of obtaining information that could not have been easily obtained by traditional DNA sampling. Our findings suggest that management strategies for this species may be much more effective if applied now when the population still shows high genetic variability. |
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Genetic diversity of the pampas deer (Ozotoceros bezoarticus) population in the Brazilian pantanal assessed by combining fresh fecal DNA analysis and a set of heterologous microsatellite lociConservationFecal DNAMicrosatellitesNon-invasive methodsPampas deerThe pampas deer (Ozotoceros bezoarticus) is close to being classified as globally threatened’, with the largest population occurring in the Brazilian Pantanal. Since capture is stressful to these animals, non-invasive sampling methods such as the use of feces can provide reliable sources of DNA. The aim of this study was to use fecal samples to evaluate the genetic variability of the Brazilian Pantanal population of pampas deer. Six heterologous microsatellite markers were used to screen 142 stool specimens. Seventy-four deer were identified, of which 50 adults were used to determine the genetic characteristics of the population. The Pantanal population showed high genetic diversity (mean number of alleles per locus = 11.5, expected heterozygosity = 0.75). This is the first investigation to characterize a South American deer species using fecal DNA and demonstrates the usefulness and efficiency of this approach, as well as the feasibility of obtaining information that could not have been easily obtained by traditional DNA sampling. Our findings suggest that management strategies for this species may be much more effective if applied now when the population still shows high genetic variability.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Núcleo de Pesquisa e Conservação de Cervídeos Departamento de Zootecnia Universidade Estadual Paulista “Júlio de Mesquita Filho” (UNESP)Departamento de Genética e Morfologia Instituto de Ciências Biológicas Universidade de BrasíliaCentro de Pesquisas Agropecuárias do Pantanal (CPAP), EmbrapaNúcleo de Pesquisa e Conservação de Cervídeos Departamento de Zootecnia Universidade Estadual Paulista “Júlio de Mesquita Filho” (UNESP)FAPESP: 2009/04253-5Universidade Estadual Paulista (Unesp)Universidade de Brasília (UnB)Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)Mantellatto, Aline Meira Bonfim [UNESP]Caparroz, RenatoChristofoletti, Maurício Durante [UNESP]Piovezan, UbiratanDuarte, José Maurício Barbanti [UNESP]2018-12-11T17:35:02Z2018-12-11T17:35:02Z2017-10-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article774-780application/pdfhttp://dx.doi.org/10.1590/1678-4685-gmb-2016-0323Genetics and Molecular Biology, v. 40, n. 4, p. 774-780, 2017.1678-46851415-4757http://hdl.handle.net/11449/17940110.1590/1678-4685-gmb-2016-0323S1415-475720170005007742-s2.0-85035795479S1415-47572017000500774.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengGenetics and Molecular Biology0,638info:eu-repo/semantics/openAccess2023-12-26T06:22:29Zoai:repositorio.unesp.br:11449/179401Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-12-26T06:22:29Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Genetic diversity of the pampas deer (Ozotoceros bezoarticus) population in the Brazilian pantanal assessed by combining fresh fecal DNA analysis and a set of heterologous microsatellite loci |
title |
Genetic diversity of the pampas deer (Ozotoceros bezoarticus) population in the Brazilian pantanal assessed by combining fresh fecal DNA analysis and a set of heterologous microsatellite loci |
spellingShingle |
Genetic diversity of the pampas deer (Ozotoceros bezoarticus) population in the Brazilian pantanal assessed by combining fresh fecal DNA analysis and a set of heterologous microsatellite loci Mantellatto, Aline Meira Bonfim [UNESP] Conservation Fecal DNA Microsatellites Non-invasive methods Pampas deer |
title_short |
Genetic diversity of the pampas deer (Ozotoceros bezoarticus) population in the Brazilian pantanal assessed by combining fresh fecal DNA analysis and a set of heterologous microsatellite loci |
title_full |
Genetic diversity of the pampas deer (Ozotoceros bezoarticus) population in the Brazilian pantanal assessed by combining fresh fecal DNA analysis and a set of heterologous microsatellite loci |
title_fullStr |
Genetic diversity of the pampas deer (Ozotoceros bezoarticus) population in the Brazilian pantanal assessed by combining fresh fecal DNA analysis and a set of heterologous microsatellite loci |
title_full_unstemmed |
Genetic diversity of the pampas deer (Ozotoceros bezoarticus) population in the Brazilian pantanal assessed by combining fresh fecal DNA analysis and a set of heterologous microsatellite loci |
title_sort |
Genetic diversity of the pampas deer (Ozotoceros bezoarticus) population in the Brazilian pantanal assessed by combining fresh fecal DNA analysis and a set of heterologous microsatellite loci |
author |
Mantellatto, Aline Meira Bonfim [UNESP] |
author_facet |
Mantellatto, Aline Meira Bonfim [UNESP] Caparroz, Renato Christofoletti, Maurício Durante [UNESP] Piovezan, Ubiratan Duarte, José Maurício Barbanti [UNESP] |
author_role |
author |
author2 |
Caparroz, Renato Christofoletti, Maurício Durante [UNESP] Piovezan, Ubiratan Duarte, José Maurício Barbanti [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade de Brasília (UnB) Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA) |
dc.contributor.author.fl_str_mv |
Mantellatto, Aline Meira Bonfim [UNESP] Caparroz, Renato Christofoletti, Maurício Durante [UNESP] Piovezan, Ubiratan Duarte, José Maurício Barbanti [UNESP] |
dc.subject.por.fl_str_mv |
Conservation Fecal DNA Microsatellites Non-invasive methods Pampas deer |
topic |
Conservation Fecal DNA Microsatellites Non-invasive methods Pampas deer |
description |
The pampas deer (Ozotoceros bezoarticus) is close to being classified as globally threatened’, with the largest population occurring in the Brazilian Pantanal. Since capture is stressful to these animals, non-invasive sampling methods such as the use of feces can provide reliable sources of DNA. The aim of this study was to use fecal samples to evaluate the genetic variability of the Brazilian Pantanal population of pampas deer. Six heterologous microsatellite markers were used to screen 142 stool specimens. Seventy-four deer were identified, of which 50 adults were used to determine the genetic characteristics of the population. The Pantanal population showed high genetic diversity (mean number of alleles per locus = 11.5, expected heterozygosity = 0.75). This is the first investigation to characterize a South American deer species using fecal DNA and demonstrates the usefulness and efficiency of this approach, as well as the feasibility of obtaining information that could not have been easily obtained by traditional DNA sampling. Our findings suggest that management strategies for this species may be much more effective if applied now when the population still shows high genetic variability. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-10-01 2018-12-11T17:35:02Z 2018-12-11T17:35:02Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/1678-4685-gmb-2016-0323 Genetics and Molecular Biology, v. 40, n. 4, p. 774-780, 2017. 1678-4685 1415-4757 http://hdl.handle.net/11449/179401 10.1590/1678-4685-gmb-2016-0323 S1415-47572017000500774 2-s2.0-85035795479 S1415-47572017000500774.pdf |
url |
http://dx.doi.org/10.1590/1678-4685-gmb-2016-0323 http://hdl.handle.net/11449/179401 |
identifier_str_mv |
Genetics and Molecular Biology, v. 40, n. 4, p. 774-780, 2017. 1678-4685 1415-4757 10.1590/1678-4685-gmb-2016-0323 S1415-47572017000500774 2-s2.0-85035795479 S1415-47572017000500774.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Genetics and Molecular Biology 0,638 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
774-780 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965417843720192 |