Designing a cocktail containing redox enzymes to improve hemicellulosic hydrolysate fermentability by microorganisms
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.enzmictec.2019.109490 http://hdl.handle.net/11449/198293 |
Resumo: | Bioproducts production using monomeric sugars derived from lignocellulosic biomass presents several challenges, such as to require a physicochemical pretreatment to improve its conversion yields. Hydrothermal lignocellulose pretreatment has several advantages and results in solid and liquid streams. The former is called hemicellulosic hydrolysate (HH), which contains inhibitory phenolic compounds and sugar degradation products that hinder microbial fermentation products from pentose sugars. Here, we developed and applied a novel enzyme process to detoxify HH. Initially, the design of experiments with different redox activities enzymes was carried out. The enzyme mixture containing the peroxidase (from Armoracia rusticana) together with superoxide dismutase (from Coptotermes gestroi) are the most effective to detoxify HH derived from sugarcane bagasse. Butanol fermentation by the bacteria Clostridium saccharoperbutylacetonicum and ethanol production by the yeast Scheffersomyces stipitis increased by 24.0× and 2.4×, respectively, relative to the untreated hemicellulosic hydrolysates. Detoxified HH was analyzed by chromatographic and spectrometric methods elucidating the mechanisms of phenolic compound modifications by enzymatic treatment. The enzyme mixture degraded and reduced the hydroxyphenyl- and feruloyl-derived units and polymerized the lignin fragments. This strategy uses biocatalysts under environmentally friendly conditions and could be applied in the fuel, food, and chemical industries. |
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Designing a cocktail containing redox enzymes to improve hemicellulosic hydrolysate fermentability by microorganismsEnzymatic detoxificationFermentation inhibitorsHydrothermal pretreatmentLignin modificationSugarcane bagasseBioproducts production using monomeric sugars derived from lignocellulosic biomass presents several challenges, such as to require a physicochemical pretreatment to improve its conversion yields. Hydrothermal lignocellulose pretreatment has several advantages and results in solid and liquid streams. The former is called hemicellulosic hydrolysate (HH), which contains inhibitory phenolic compounds and sugar degradation products that hinder microbial fermentation products from pentose sugars. Here, we developed and applied a novel enzyme process to detoxify HH. Initially, the design of experiments with different redox activities enzymes was carried out. The enzyme mixture containing the peroxidase (from Armoracia rusticana) together with superoxide dismutase (from Coptotermes gestroi) are the most effective to detoxify HH derived from sugarcane bagasse. Butanol fermentation by the bacteria Clostridium saccharoperbutylacetonicum and ethanol production by the yeast Scheffersomyces stipitis increased by 24.0× and 2.4×, respectively, relative to the untreated hemicellulosic hydrolysates. Detoxified HH was analyzed by chromatographic and spectrometric methods elucidating the mechanisms of phenolic compound modifications by enzymatic treatment. The enzyme mixture degraded and reduced the hydroxyphenyl- and feruloyl-derived units and polymerized the lignin fragments. This strategy uses biocatalysts under environmentally friendly conditions and could be applied in the fuel, food, and chemical industries.Laboratório Nacional de de Biorrenováveis (LNBR) Centro Nacional de Pesquisa em Energia e Materiais (CNPEM)Programa de Pós-Graduação em Biociências e Tecnologia de Produtos Bioativos (BTPB) Universidade Estadual de Campinas – UNICAMPNovozymes America LatinaPrograma em Bioenergia Faculdade de Engenharia de Alimentos Universidade Estadual de Campinas (UNICAMP)Departamento de Bioprocessos e Biotecnologia Faculdade de Ciências Agronômicas Universidade Estadual Paulista Júlio de Mesquita Filho – UNESP, Campus Botucatu, BotucatuFaculdade de Engenharia Química (FEQ) Universidade Estadual de Campinas (UNICAMP)Laboratório de Bioquimica Geral e de Microorganismos Instituto de Biociências Universidade Federal de Mato Grosso do SulInstituto de Biociências Letras e Ciências Exatas (IBILCE) Universidade Estadual PaulistaPrograma em Processos Tecnológicos e Ambientais Universidade de Sorocaba (UNISO)Departamento de Bioprocessos e Biotecnologia Faculdade de Ciências Agronômicas Universidade Estadual Paulista Júlio de Mesquita Filho – UNESP, Campus Botucatu, BotucatuInstituto de Biociências Letras e Ciências Exatas (IBILCE) Universidade Estadual PaulistaCentro Nacional de Pesquisa em Energia e Materiais (CNPEM)Universidade Estadual de Campinas (UNICAMP)Novozymes America LatinaUniversidade Estadual Paulista (Unesp)Universidade Federal de Mato Grosso do Sul (UFMS)Universidade de Sorocaba (UNISO)Tramontina, RobsonBrenelli, Lívia BeatrizSousa, AmandaAlves, RafaelZetty Arenas, Ana MariaNascimento, Viviane MarcosRabelo, Sarita Cândida [UNESP]Freitas, SindéliaRuller, Roberto [UNESP]Squina, Fabio Marcio2020-12-12T01:08:47Z2020-12-12T01:08:47Z2020-04-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.enzmictec.2019.109490Enzyme and Microbial Technology, v. 135.1879-09090141-0229http://hdl.handle.net/11449/19829310.1016/j.enzmictec.2019.1094902-s2.0-85076670264Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengEnzyme and Microbial Technologyinfo:eu-repo/semantics/openAccess2021-10-23T10:18:11Zoai:repositorio.unesp.br:11449/198293Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T10:18:11Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Designing a cocktail containing redox enzymes to improve hemicellulosic hydrolysate fermentability by microorganisms |
title |
Designing a cocktail containing redox enzymes to improve hemicellulosic hydrolysate fermentability by microorganisms |
spellingShingle |
Designing a cocktail containing redox enzymes to improve hemicellulosic hydrolysate fermentability by microorganisms Tramontina, Robson Enzymatic detoxification Fermentation inhibitors Hydrothermal pretreatment Lignin modification Sugarcane bagasse |
title_short |
Designing a cocktail containing redox enzymes to improve hemicellulosic hydrolysate fermentability by microorganisms |
title_full |
Designing a cocktail containing redox enzymes to improve hemicellulosic hydrolysate fermentability by microorganisms |
title_fullStr |
Designing a cocktail containing redox enzymes to improve hemicellulosic hydrolysate fermentability by microorganisms |
title_full_unstemmed |
Designing a cocktail containing redox enzymes to improve hemicellulosic hydrolysate fermentability by microorganisms |
title_sort |
Designing a cocktail containing redox enzymes to improve hemicellulosic hydrolysate fermentability by microorganisms |
author |
Tramontina, Robson |
author_facet |
Tramontina, Robson Brenelli, Lívia Beatriz Sousa, Amanda Alves, Rafael Zetty Arenas, Ana Maria Nascimento, Viviane Marcos Rabelo, Sarita Cândida [UNESP] Freitas, Sindélia Ruller, Roberto [UNESP] Squina, Fabio Marcio |
author_role |
author |
author2 |
Brenelli, Lívia Beatriz Sousa, Amanda Alves, Rafael Zetty Arenas, Ana Maria Nascimento, Viviane Marcos Rabelo, Sarita Cândida [UNESP] Freitas, Sindélia Ruller, Roberto [UNESP] Squina, Fabio Marcio |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Centro Nacional de Pesquisa em Energia e Materiais (CNPEM) Universidade Estadual de Campinas (UNICAMP) Novozymes America Latina Universidade Estadual Paulista (Unesp) Universidade Federal de Mato Grosso do Sul (UFMS) Universidade de Sorocaba (UNISO) |
dc.contributor.author.fl_str_mv |
Tramontina, Robson Brenelli, Lívia Beatriz Sousa, Amanda Alves, Rafael Zetty Arenas, Ana Maria Nascimento, Viviane Marcos Rabelo, Sarita Cândida [UNESP] Freitas, Sindélia Ruller, Roberto [UNESP] Squina, Fabio Marcio |
dc.subject.por.fl_str_mv |
Enzymatic detoxification Fermentation inhibitors Hydrothermal pretreatment Lignin modification Sugarcane bagasse |
topic |
Enzymatic detoxification Fermentation inhibitors Hydrothermal pretreatment Lignin modification Sugarcane bagasse |
description |
Bioproducts production using monomeric sugars derived from lignocellulosic biomass presents several challenges, such as to require a physicochemical pretreatment to improve its conversion yields. Hydrothermal lignocellulose pretreatment has several advantages and results in solid and liquid streams. The former is called hemicellulosic hydrolysate (HH), which contains inhibitory phenolic compounds and sugar degradation products that hinder microbial fermentation products from pentose sugars. Here, we developed and applied a novel enzyme process to detoxify HH. Initially, the design of experiments with different redox activities enzymes was carried out. The enzyme mixture containing the peroxidase (from Armoracia rusticana) together with superoxide dismutase (from Coptotermes gestroi) are the most effective to detoxify HH derived from sugarcane bagasse. Butanol fermentation by the bacteria Clostridium saccharoperbutylacetonicum and ethanol production by the yeast Scheffersomyces stipitis increased by 24.0× and 2.4×, respectively, relative to the untreated hemicellulosic hydrolysates. Detoxified HH was analyzed by chromatographic and spectrometric methods elucidating the mechanisms of phenolic compound modifications by enzymatic treatment. The enzyme mixture degraded and reduced the hydroxyphenyl- and feruloyl-derived units and polymerized the lignin fragments. This strategy uses biocatalysts under environmentally friendly conditions and could be applied in the fuel, food, and chemical industries. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-12T01:08:47Z 2020-12-12T01:08:47Z 2020-04-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.enzmictec.2019.109490 Enzyme and Microbial Technology, v. 135. 1879-0909 0141-0229 http://hdl.handle.net/11449/198293 10.1016/j.enzmictec.2019.109490 2-s2.0-85076670264 |
url |
http://dx.doi.org/10.1016/j.enzmictec.2019.109490 http://hdl.handle.net/11449/198293 |
identifier_str_mv |
Enzyme and Microbial Technology, v. 135. 1879-0909 0141-0229 10.1016/j.enzmictec.2019.109490 2-s2.0-85076670264 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Enzyme and Microbial Technology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965002409443328 |