Comparative cytogenetics of serrasalmidae (Teleostei: Characiformes): The relationship between chromosomal evolution and molecular phylogenies
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1371/journal.pone.0258003 http://hdl.handle.net/11449/222613 |
Resumo: | Serrasalmidae has high morphological and chromosomal diversity. Based on molecular hypotheses, the family is currently divided into two subfamilies, Colossomatinae and Serrasalminae, with Serrasalminae composed of two tribes: Myleini (comprising most of pacus species) and Serrasalmini (represented by Metynnis, Catoprion, and remaining piranha’s genera). This study aimed to analyze species of the tribes Myleini (Myloplus asterias, M. lobatus, M. rubripinnis, M. schomburgki, and Tometes camunani) and Serrasalmini (Metynnis cuiaba, M. hypsauchen, and M. longipinnis) using classical and molecular cytogenetic techniques in order to understand the chromosomal evolution of the family. The four species of the genus Myloplus and T. camunani presented 2n = 58 chromosomes, while the species of Metynnis presented 2n = 62 chromosomes. The distribution of heterochromatin occurred predominantly in pericentromeric regions in all species. Tometes camunani and Myloplus spp. presented only one site with 5S rDNA. Multiple markers of 18S rDNA were observed in T. camunani, M. asterias, M. lobatus, M. rubripinnis, and M. schomburgkii. For Metynnis, however, synteny of the 18S and 5S rDNA was observed in the three species, in addition to an additional 5S marker in M. longipinnis. These data, when superimposed on the phylogeny of the family, suggest a tendency to increase the diploid chromosome number from 54 to 62 chromosomes, which occurred in a nonlinear manner and is the result of several chromosomal rearrangements. In addition, the different karyotype formulas and locations of ribosomal sequences can be used as cytotaxonomic markers and assist in the identification of species. |
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Comparative cytogenetics of serrasalmidae (Teleostei: Characiformes): The relationship between chromosomal evolution and molecular phylogeniesSerrasalmidae has high morphological and chromosomal diversity. Based on molecular hypotheses, the family is currently divided into two subfamilies, Colossomatinae and Serrasalminae, with Serrasalminae composed of two tribes: Myleini (comprising most of pacus species) and Serrasalmini (represented by Metynnis, Catoprion, and remaining piranha’s genera). This study aimed to analyze species of the tribes Myleini (Myloplus asterias, M. lobatus, M. rubripinnis, M. schomburgki, and Tometes camunani) and Serrasalmini (Metynnis cuiaba, M. hypsauchen, and M. longipinnis) using classical and molecular cytogenetic techniques in order to understand the chromosomal evolution of the family. The four species of the genus Myloplus and T. camunani presented 2n = 58 chromosomes, while the species of Metynnis presented 2n = 62 chromosomes. The distribution of heterochromatin occurred predominantly in pericentromeric regions in all species. Tometes camunani and Myloplus spp. presented only one site with 5S rDNA. Multiple markers of 18S rDNA were observed in T. camunani, M. asterias, M. lobatus, M. rubripinnis, and M. schomburgkii. For Metynnis, however, synteny of the 18S and 5S rDNA was observed in the three species, in addition to an additional 5S marker in M. longipinnis. These data, when superimposed on the phylogeny of the family, suggest a tendency to increase the diploid chromosome number from 54 to 62 chromosomes, which occurred in a nonlinear manner and is the result of several chromosomal rearrangements. In addition, the different karyotype formulas and locations of ribosomal sequences can be used as cytotaxonomic markers and assist in the identification of species.Fundação de Amparo à Pesquisa do Estado do AmazonasPrograma de Pós-Graduação em Genética Conservação e Biologia Evolutiva Instituto Nacional de Pesquisas da Amazônia Petrópolis, AmazonasCentro de Ciências da Saúde Universidade Federal de Roraima, Avenida Capitão Ene Garcêz, RRCoordenação de Pesquisas em Biodiversidade Instituto Nacional de Pesquisas da Amazônia Petrópolis, AmazonasDepartamento de Biologia Estrutural e Funcional Instituto de Biociências Universidade Estadual Paulista “Júlio de Mesquita Filho”, São PauloDepartamento de Biologia Estrutural e Funcional Instituto de Biociências Universidade Estadual Paulista “Júlio de Mesquita Filho”, São PauloPetrópolisUniversidade Federal de RoraimaUniversidade Estadual Paulista (UNESP)Favarato, Ramon MarinRibeiro, Leila BragaCampos, AlberPorto, Jorge Ivan RebeloNakayama, Celeste MutukoOta, Rafaela Priscila [UNESP]Feldberg, Eliana2022-04-28T19:45:47Z2022-04-28T19:45:47Z2021-10-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1371/journal.pone.0258003PLoS ONE, v. 16, n. 10 October, 2021.1932-6203http://hdl.handle.net/11449/22261310.1371/journal.pone.02580032-s2.0-85116868441Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPLoS ONEinfo:eu-repo/semantics/openAccess2022-04-28T19:45:48Zoai:repositorio.unesp.br:11449/222613Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462022-04-28T19:45:48Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Comparative cytogenetics of serrasalmidae (Teleostei: Characiformes): The relationship between chromosomal evolution and molecular phylogenies |
title |
Comparative cytogenetics of serrasalmidae (Teleostei: Characiformes): The relationship between chromosomal evolution and molecular phylogenies |
spellingShingle |
Comparative cytogenetics of serrasalmidae (Teleostei: Characiformes): The relationship between chromosomal evolution and molecular phylogenies Favarato, Ramon Marin |
title_short |
Comparative cytogenetics of serrasalmidae (Teleostei: Characiformes): The relationship between chromosomal evolution and molecular phylogenies |
title_full |
Comparative cytogenetics of serrasalmidae (Teleostei: Characiformes): The relationship between chromosomal evolution and molecular phylogenies |
title_fullStr |
Comparative cytogenetics of serrasalmidae (Teleostei: Characiformes): The relationship between chromosomal evolution and molecular phylogenies |
title_full_unstemmed |
Comparative cytogenetics of serrasalmidae (Teleostei: Characiformes): The relationship between chromosomal evolution and molecular phylogenies |
title_sort |
Comparative cytogenetics of serrasalmidae (Teleostei: Characiformes): The relationship between chromosomal evolution and molecular phylogenies |
author |
Favarato, Ramon Marin |
author_facet |
Favarato, Ramon Marin Ribeiro, Leila Braga Campos, Alber Porto, Jorge Ivan Rebelo Nakayama, Celeste Mutuko Ota, Rafaela Priscila [UNESP] Feldberg, Eliana |
author_role |
author |
author2 |
Ribeiro, Leila Braga Campos, Alber Porto, Jorge Ivan Rebelo Nakayama, Celeste Mutuko Ota, Rafaela Priscila [UNESP] Feldberg, Eliana |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Petrópolis Universidade Federal de Roraima Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Favarato, Ramon Marin Ribeiro, Leila Braga Campos, Alber Porto, Jorge Ivan Rebelo Nakayama, Celeste Mutuko Ota, Rafaela Priscila [UNESP] Feldberg, Eliana |
description |
Serrasalmidae has high morphological and chromosomal diversity. Based on molecular hypotheses, the family is currently divided into two subfamilies, Colossomatinae and Serrasalminae, with Serrasalminae composed of two tribes: Myleini (comprising most of pacus species) and Serrasalmini (represented by Metynnis, Catoprion, and remaining piranha’s genera). This study aimed to analyze species of the tribes Myleini (Myloplus asterias, M. lobatus, M. rubripinnis, M. schomburgki, and Tometes camunani) and Serrasalmini (Metynnis cuiaba, M. hypsauchen, and M. longipinnis) using classical and molecular cytogenetic techniques in order to understand the chromosomal evolution of the family. The four species of the genus Myloplus and T. camunani presented 2n = 58 chromosomes, while the species of Metynnis presented 2n = 62 chromosomes. The distribution of heterochromatin occurred predominantly in pericentromeric regions in all species. Tometes camunani and Myloplus spp. presented only one site with 5S rDNA. Multiple markers of 18S rDNA were observed in T. camunani, M. asterias, M. lobatus, M. rubripinnis, and M. schomburgkii. For Metynnis, however, synteny of the 18S and 5S rDNA was observed in the three species, in addition to an additional 5S marker in M. longipinnis. These data, when superimposed on the phylogeny of the family, suggest a tendency to increase the diploid chromosome number from 54 to 62 chromosomes, which occurred in a nonlinear manner and is the result of several chromosomal rearrangements. In addition, the different karyotype formulas and locations of ribosomal sequences can be used as cytotaxonomic markers and assist in the identification of species. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-10-01 2022-04-28T19:45:47Z 2022-04-28T19:45:47Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1371/journal.pone.0258003 PLoS ONE, v. 16, n. 10 October, 2021. 1932-6203 http://hdl.handle.net/11449/222613 10.1371/journal.pone.0258003 2-s2.0-85116868441 |
url |
http://dx.doi.org/10.1371/journal.pone.0258003 http://hdl.handle.net/11449/222613 |
identifier_str_mv |
PLoS ONE, v. 16, n. 10 October, 2021. 1932-6203 10.1371/journal.pone.0258003 2-s2.0-85116868441 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
PLoS ONE |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1797789923715055616 |