Fibroblast growth factor 2 regulates cumulus differentiation under the control of the oocyte
Autor(a) principal: | |
---|---|
Data de Publicação: | 2019 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s10815-019-01436-7 http://hdl.handle.net/11449/186763 |
Resumo: | PurposeWe first assessed regulation of FGF2 expression in cumulus cells by FSH and oocyte-secreted factors during in vitro maturation (IVM). Then, we tested the hypothesis that FGF2 regulates meiotic progression, cumulus expansion, and apoptosis in cumulus-oocyte complexes (COC) undergoing IVM.MethodsIn vitro maturation of bovine COC was utilized as a model to assess regulation of FGF2 expression by FSH and oocyte-secreted factors (via microsurgical removal of the oocyte), as well as effects of graded doses of FGF2 on meiotic progression, degree of cumulus expansion, dissociation of cumulus cells, and cumulus cells apoptosis. Expression of genes regulating functional endpoints altered by FGF2 treatment was assessed in cumulus cells by real-time PCR. Cultures were replicated 4-5 times and effects of treatments were tested by ANOVA.ResultsFGF2 mRNA expression was increased by FSH and oocyte-secreted factors during IVM. Addition of FGF2 to the IVM medium advanced meiosis resumption, decreased the ease with which cumulus cells were dissociated, and inhibited cumulus cells apoptosis. Decreased cumulus dissociation was accompanied by decreased expression of TNFAIP6.ConclusionsThis is the first study showing that FGF2 expression is regulated by the oocyte in cumulus cells. Moreover, we report novel effects of FGF2 on cumulus cell survival and extracellular matrix (ECM) quality during IVM that may favor acquisition of developmental competence and suggest physiological roles during the final steps of COC differentiation. |
id |
UNSP_f40d9c2c3005dee6d10965de5bd359c7 |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/186763 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Fibroblast growth factor 2 regulates cumulus differentiation under the control of the oocyteFGF2IVMOocyte-secreted factorsMeiosisCumulus expansionApoptosisPurposeWe first assessed regulation of FGF2 expression in cumulus cells by FSH and oocyte-secreted factors during in vitro maturation (IVM). Then, we tested the hypothesis that FGF2 regulates meiotic progression, cumulus expansion, and apoptosis in cumulus-oocyte complexes (COC) undergoing IVM.MethodsIn vitro maturation of bovine COC was utilized as a model to assess regulation of FGF2 expression by FSH and oocyte-secreted factors (via microsurgical removal of the oocyte), as well as effects of graded doses of FGF2 on meiotic progression, degree of cumulus expansion, dissociation of cumulus cells, and cumulus cells apoptosis. Expression of genes regulating functional endpoints altered by FGF2 treatment was assessed in cumulus cells by real-time PCR. Cultures were replicated 4-5 times and effects of treatments were tested by ANOVA.ResultsFGF2 mRNA expression was increased by FSH and oocyte-secreted factors during IVM. Addition of FGF2 to the IVM medium advanced meiosis resumption, decreased the ease with which cumulus cells were dissociated, and inhibited cumulus cells apoptosis. Decreased cumulus dissociation was accompanied by decreased expression of TNFAIP6.ConclusionsThis is the first study showing that FGF2 expression is regulated by the oocyte in cumulus cells. Moreover, we report novel effects of FGF2 on cumulus cell survival and extracellular matrix (ECM) quality during IVM that may favor acquisition of developmental competence and suggest physiological roles during the final steps of COC differentiation.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Univ Estadual Paulista, Dept Fisiol, Inst Biociencias, BR-18618970 Sao Paulo, BrazilUniv Montreal, Fac Med Vet, Ctr Rech Reprod & Fertilite, St Hyacinthe, PQ J2S 7C6, CanadaUniv Estadual Paulista, Dept Fisiol, Inst Biociencias, BR-18618970 Sao Paulo, BrazilFAPESP: 12/06417-8CNPq: 312521/2014-6SpringerUniversidade Estadual Paulista (Unesp)Univ MontrealBarros, Rodrigo G. [UNESP]Lima, Paula F. [UNESP]Soares, Ana Caroline S. [UNESP]Sanches, Lorena [UNESP]Price, Christopher A.Buratini, Jose [UNESP]2019-10-06T02:14:59Z2019-10-06T02:14:59Z2019-05-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article905-913http://dx.doi.org/10.1007/s10815-019-01436-7Journal Of Assisted Reproduction And Genetics. New York: Springer/plenum Publishers, v. 36, n. 5, p. 905-913, 2019.1058-0468http://hdl.handle.net/11449/18676310.1007/s10815-019-01436-7WOS:000469445600010Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal Of Assisted Reproduction And Geneticsinfo:eu-repo/semantics/openAccess2021-10-22T22:17:24Zoai:repositorio.unesp.br:11449/186763Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-22T22:17:24Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Fibroblast growth factor 2 regulates cumulus differentiation under the control of the oocyte |
title |
Fibroblast growth factor 2 regulates cumulus differentiation under the control of the oocyte |
spellingShingle |
Fibroblast growth factor 2 regulates cumulus differentiation under the control of the oocyte Barros, Rodrigo G. [UNESP] FGF2 IVM Oocyte-secreted factors Meiosis Cumulus expansion Apoptosis |
title_short |
Fibroblast growth factor 2 regulates cumulus differentiation under the control of the oocyte |
title_full |
Fibroblast growth factor 2 regulates cumulus differentiation under the control of the oocyte |
title_fullStr |
Fibroblast growth factor 2 regulates cumulus differentiation under the control of the oocyte |
title_full_unstemmed |
Fibroblast growth factor 2 regulates cumulus differentiation under the control of the oocyte |
title_sort |
Fibroblast growth factor 2 regulates cumulus differentiation under the control of the oocyte |
author |
Barros, Rodrigo G. [UNESP] |
author_facet |
Barros, Rodrigo G. [UNESP] Lima, Paula F. [UNESP] Soares, Ana Caroline S. [UNESP] Sanches, Lorena [UNESP] Price, Christopher A. Buratini, Jose [UNESP] |
author_role |
author |
author2 |
Lima, Paula F. [UNESP] Soares, Ana Caroline S. [UNESP] Sanches, Lorena [UNESP] Price, Christopher A. Buratini, Jose [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Univ Montreal |
dc.contributor.author.fl_str_mv |
Barros, Rodrigo G. [UNESP] Lima, Paula F. [UNESP] Soares, Ana Caroline S. [UNESP] Sanches, Lorena [UNESP] Price, Christopher A. Buratini, Jose [UNESP] |
dc.subject.por.fl_str_mv |
FGF2 IVM Oocyte-secreted factors Meiosis Cumulus expansion Apoptosis |
topic |
FGF2 IVM Oocyte-secreted factors Meiosis Cumulus expansion Apoptosis |
description |
PurposeWe first assessed regulation of FGF2 expression in cumulus cells by FSH and oocyte-secreted factors during in vitro maturation (IVM). Then, we tested the hypothesis that FGF2 regulates meiotic progression, cumulus expansion, and apoptosis in cumulus-oocyte complexes (COC) undergoing IVM.MethodsIn vitro maturation of bovine COC was utilized as a model to assess regulation of FGF2 expression by FSH and oocyte-secreted factors (via microsurgical removal of the oocyte), as well as effects of graded doses of FGF2 on meiotic progression, degree of cumulus expansion, dissociation of cumulus cells, and cumulus cells apoptosis. Expression of genes regulating functional endpoints altered by FGF2 treatment was assessed in cumulus cells by real-time PCR. Cultures were replicated 4-5 times and effects of treatments were tested by ANOVA.ResultsFGF2 mRNA expression was increased by FSH and oocyte-secreted factors during IVM. Addition of FGF2 to the IVM medium advanced meiosis resumption, decreased the ease with which cumulus cells were dissociated, and inhibited cumulus cells apoptosis. Decreased cumulus dissociation was accompanied by decreased expression of TNFAIP6.ConclusionsThis is the first study showing that FGF2 expression is regulated by the oocyte in cumulus cells. Moreover, we report novel effects of FGF2 on cumulus cell survival and extracellular matrix (ECM) quality during IVM that may favor acquisition of developmental competence and suggest physiological roles during the final steps of COC differentiation. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-06T02:14:59Z 2019-10-06T02:14:59Z 2019-05-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s10815-019-01436-7 Journal Of Assisted Reproduction And Genetics. New York: Springer/plenum Publishers, v. 36, n. 5, p. 905-913, 2019. 1058-0468 http://hdl.handle.net/11449/186763 10.1007/s10815-019-01436-7 WOS:000469445600010 |
url |
http://dx.doi.org/10.1007/s10815-019-01436-7 http://hdl.handle.net/11449/186763 |
identifier_str_mv |
Journal Of Assisted Reproduction And Genetics. New York: Springer/plenum Publishers, v. 36, n. 5, p. 905-913, 2019. 1058-0468 10.1007/s10815-019-01436-7 WOS:000469445600010 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal Of Assisted Reproduction And Genetics |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
905-913 |
dc.publisher.none.fl_str_mv |
Springer |
publisher.none.fl_str_mv |
Springer |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1797790084257284096 |