Preparative isolation of a novel flavonoid from an infusion of Byrsonima basiloba leaves by high-speed counter-current chromatography

• Autor(a) principal: Sannomiya, Miriam [UNESP]
• Data de Publicação: 2007
• Outros Autores: Figueiredo, Maria E. [UNESP], Da Silva, Marcelo A. [UNESP], Rodrigues, Clenilson M. [UNESP], Dos Santos, Lourdes C. [UNESP], Souza-Brito, Alba R.M., Vilegas, Wagner [UNESP]
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://dx.doi.org/10.1177/1934578x0700200808; http://hdl.handle.net/11449/226479
• topic: Byrsonima basiloba; Counter-current chromatography; Flavonoids; Gradient elution

A method for the isolation of three compounds from the infusion of leaves of Byrsonima basiloba A. Juss. by high-speed counter-current chromatography (HSCCC) was developed. This technique led to the separation of a novel compound, quercetin 3-O-α-L-rhamnopyranosyl-(1→3)-O-[α-L- rhamnopyranosyl-(1→6)]-β-D-allopyranoside, and two known compounds quercetin-3-O-α-L-rhamnopyranosyl-(1→6)-β-D-galactopyranoside and (+)-catechin in 4 h with purities of over 92%. The structures of the compounds were determined by one- and two-dimensional NMR spectroscopy and HPLC.