Estudo de bactérias do gênero gluconobacter: Isolamento, purificação, identificação fenotípica e molecular
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://hdl.handle.net/11449/225856 |
Resumo: | The importance of the study of acetic bacteria, on species of the Gluconobacter genus is based on its industrial application, as these possess the capacity of bioconversion of sorbitol to sorbose, enabling the process of vitamin C production. The study involved samples collected in industries of soft drinks, flowers, fruits and honey, followed by purification, phenotypic identification, molecular identification with the use of primer defined from Nucleotide Sequence Database consultation. Strains preserved were identified as members of the Acetobacteraceae family, Gluconobacter genus. 110 strains had been isolated of substrate: Pyrostegia venusta (ker-gawler), honey, Vitis vinifera (grape), Pyrus communis (pear), Malus sp. (apple) and in two samples of soft drinks. Of this total 57 strains had been recovered in manitol medium (manitol, yeast extract, peptone), 12 in YMG medium (glucose, manitol, yeast extract, ethanol, acetic acid), 41 in enrichment medium (De Ley and Swings) and later in the GYC medium (glucose, yeast extract and calcium carbonate). 68 strains were identified as Gram negative bacilli rods. Of these, 31 were characterized biochemically as belonging to the Acetobacteriaceae family as they were catalase positive, oxidase negative and producers of acid from glucose. The characterization of these strains was complemented with the biochemistry tests: gelatin liquefaction, nitrate reduction, indole and H2S production, oxidation of ethanol to acetic acid and molecular tests for genus identification. Only eight strains were characterized as pertaining to the Gluconobacter genus. The strains are maintained in collection cultures at the Microbiology Laboratory of the Biology Department at the São Paulo State University (UNESP) in Assis, stored in malt extract at -196 °C. |
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Estudo de bactérias do gênero gluconobacter: Isolamento, purificação, identificação fenotípica e molecularStudy of bacteria gluconobacter sp.: Isolation, purification, phenotypic and molecular identificationGluconobacterIdentificationIsolationMolecular markersPCRThe importance of the study of acetic bacteria, on species of the Gluconobacter genus is based on its industrial application, as these possess the capacity of bioconversion of sorbitol to sorbose, enabling the process of vitamin C production. The study involved samples collected in industries of soft drinks, flowers, fruits and honey, followed by purification, phenotypic identification, molecular identification with the use of primer defined from Nucleotide Sequence Database consultation. Strains preserved were identified as members of the Acetobacteraceae family, Gluconobacter genus. 110 strains had been isolated of substrate: Pyrostegia venusta (ker-gawler), honey, Vitis vinifera (grape), Pyrus communis (pear), Malus sp. (apple) and in two samples of soft drinks. Of this total 57 strains had been recovered in manitol medium (manitol, yeast extract, peptone), 12 in YMG medium (glucose, manitol, yeast extract, ethanol, acetic acid), 41 in enrichment medium (De Ley and Swings) and later in the GYC medium (glucose, yeast extract and calcium carbonate). 68 strains were identified as Gram negative bacilli rods. Of these, 31 were characterized biochemically as belonging to the Acetobacteriaceae family as they were catalase positive, oxidase negative and producers of acid from glucose. The characterization of these strains was complemented with the biochemistry tests: gelatin liquefaction, nitrate reduction, indole and H2S production, oxidation of ethanol to acetic acid and molecular tests for genus identification. Only eight strains were characterized as pertaining to the Gluconobacter genus. The strains are maintained in collection cultures at the Microbiology Laboratory of the Biology Department at the São Paulo State University (UNESP) in Assis, stored in malt extract at -196 °C.Departamento de Ciências Biológicas Universidade Estadual Paulista - UNESP, Assis, SPDepartamento de Saúde ADR - Biomavale, Rua Dr. Geraldo Nogueira Leite, 1735, CEP 19800-000, Assis, SPInstituto de Saúde Universidade Paulista - UNIP, Assis, SPDepartamento de Ciências Biológicas Universidade Estadual Paulista - UNESP, Assis, SPUniversidade Estadual Paulista (UNESP)ADR - BiomavaleUniversidade Paulista - UNIPde Oliveira, André Luiz Dorini [UNESP]Santos Jr., Vitório [UNESP]Liotti, Rhavena Graziela [UNESP]Zilioli, Estevão [UNESP]Spinosa, Wilma Aparecida [UNESP]Ribeiro-Paes, João Tadeu [UNESP]2022-04-28T21:01:20Z2022-04-28T21:01:20Z2010-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article106-112Ciencia e Tecnologia de Alimentos, v. 30, n. 1, p. 106-112, 2010.0101-20611678-457Xhttp://hdl.handle.net/11449/2258562-s2.0-77952830377Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPporCiencia e Tecnologia de Alimentosinfo:eu-repo/semantics/openAccess2022-04-28T21:01:20Zoai:repositorio.unesp.br:11449/225856Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462022-04-28T21:01:20Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Estudo de bactérias do gênero gluconobacter: Isolamento, purificação, identificação fenotípica e molecular Study of bacteria gluconobacter sp.: Isolation, purification, phenotypic and molecular identification |
title |
Estudo de bactérias do gênero gluconobacter: Isolamento, purificação, identificação fenotípica e molecular |
spellingShingle |
Estudo de bactérias do gênero gluconobacter: Isolamento, purificação, identificação fenotípica e molecular de Oliveira, André Luiz Dorini [UNESP] Gluconobacter Identification Isolation Molecular markers PCR |
title_short |
Estudo de bactérias do gênero gluconobacter: Isolamento, purificação, identificação fenotípica e molecular |
title_full |
Estudo de bactérias do gênero gluconobacter: Isolamento, purificação, identificação fenotípica e molecular |
title_fullStr |
Estudo de bactérias do gênero gluconobacter: Isolamento, purificação, identificação fenotípica e molecular |
title_full_unstemmed |
Estudo de bactérias do gênero gluconobacter: Isolamento, purificação, identificação fenotípica e molecular |
title_sort |
Estudo de bactérias do gênero gluconobacter: Isolamento, purificação, identificação fenotípica e molecular |
author |
de Oliveira, André Luiz Dorini [UNESP] |
author_facet |
de Oliveira, André Luiz Dorini [UNESP] Santos Jr., Vitório [UNESP] Liotti, Rhavena Graziela [UNESP] Zilioli, Estevão [UNESP] Spinosa, Wilma Aparecida [UNESP] Ribeiro-Paes, João Tadeu [UNESP] |
author_role |
author |
author2 |
Santos Jr., Vitório [UNESP] Liotti, Rhavena Graziela [UNESP] Zilioli, Estevão [UNESP] Spinosa, Wilma Aparecida [UNESP] Ribeiro-Paes, João Tadeu [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) ADR - Biomavale Universidade Paulista - UNIP |
dc.contributor.author.fl_str_mv |
de Oliveira, André Luiz Dorini [UNESP] Santos Jr., Vitório [UNESP] Liotti, Rhavena Graziela [UNESP] Zilioli, Estevão [UNESP] Spinosa, Wilma Aparecida [UNESP] Ribeiro-Paes, João Tadeu [UNESP] |
dc.subject.por.fl_str_mv |
Gluconobacter Identification Isolation Molecular markers PCR |
topic |
Gluconobacter Identification Isolation Molecular markers PCR |
description |
The importance of the study of acetic bacteria, on species of the Gluconobacter genus is based on its industrial application, as these possess the capacity of bioconversion of sorbitol to sorbose, enabling the process of vitamin C production. The study involved samples collected in industries of soft drinks, flowers, fruits and honey, followed by purification, phenotypic identification, molecular identification with the use of primer defined from Nucleotide Sequence Database consultation. Strains preserved were identified as members of the Acetobacteraceae family, Gluconobacter genus. 110 strains had been isolated of substrate: Pyrostegia venusta (ker-gawler), honey, Vitis vinifera (grape), Pyrus communis (pear), Malus sp. (apple) and in two samples of soft drinks. Of this total 57 strains had been recovered in manitol medium (manitol, yeast extract, peptone), 12 in YMG medium (glucose, manitol, yeast extract, ethanol, acetic acid), 41 in enrichment medium (De Ley and Swings) and later in the GYC medium (glucose, yeast extract and calcium carbonate). 68 strains were identified as Gram negative bacilli rods. Of these, 31 were characterized biochemically as belonging to the Acetobacteriaceae family as they were catalase positive, oxidase negative and producers of acid from glucose. The characterization of these strains was complemented with the biochemistry tests: gelatin liquefaction, nitrate reduction, indole and H2S production, oxidation of ethanol to acetic acid and molecular tests for genus identification. Only eight strains were characterized as pertaining to the Gluconobacter genus. The strains are maintained in collection cultures at the Microbiology Laboratory of the Biology Department at the São Paulo State University (UNESP) in Assis, stored in malt extract at -196 °C. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-01-01 2022-04-28T21:01:20Z 2022-04-28T21:01:20Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Ciencia e Tecnologia de Alimentos, v. 30, n. 1, p. 106-112, 2010. 0101-2061 1678-457X http://hdl.handle.net/11449/225856 2-s2.0-77952830377 |
identifier_str_mv |
Ciencia e Tecnologia de Alimentos, v. 30, n. 1, p. 106-112, 2010. 0101-2061 1678-457X 2-s2.0-77952830377 |
url |
http://hdl.handle.net/11449/225856 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
Ciencia e Tecnologia de Alimentos |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
106-112 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1799964916881293312 |