Transcriptional profile of a bioethanol production contaminant Candida tropicalis
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1186/s13568-018-0693-1 http://hdl.handle.net/11449/189795 |
Resumo: | The fermentation process is widely used in the industry for bioethanol production. Even though it is widely used, microbial contamination is unpredictable and difficult to control. The problem of reduced productivity is directly linked to competition for nutrients during contamination. Yeasts representing the Candida species are frequently isolated contaminants. Elucidating the behavior of a contaminant during the fermentation cycle is essential for combatting the contamination. Consequently, the aim of the current study was to better understand the functional and transcriptional behavior of a contaminating yeast Candida tropicalis. We used a global RNA sequencing approach (RNA-seq/MiSeq) to analyze gene expression. Genes with significantly repressed or induced expression, and related to the fermentations process, such as sugar transport, pyruvate decarboxylase, amino acid metabolism, membrane, tolerance to high concentrations of ethanol and temperatures, nutrient suppression), and transcription-linked processes, were identified. The expression pattern suggested that the functional and transcriptional behavior of the contaminating yeast during fermentation for bioethanol production is similar to that of the standard yeast Saccharomyces cerevisiae. In addition, the analysis confirmed that C. tropicalis is an important contaminant of the alcoholic fermentation process, generating bioethanol and viability through its tolerance to all the adversities of a fermentation process essential for the production of bioethanol. According on the gene expression profile, many of these mechanisms are similar to those of S. cerevisiae strains currently used for bioethanol production. These mechanisms can inform studies on antimicrobials, to combat yeast contamination during industrial bioethanol production. |
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Transcriptional profile of a bioethanol production contaminant Candida tropicalisAlcoholic fermentationBioethanolCandida tropicalisContaminantRNA-seqThe fermentation process is widely used in the industry for bioethanol production. Even though it is widely used, microbial contamination is unpredictable and difficult to control. The problem of reduced productivity is directly linked to competition for nutrients during contamination. Yeasts representing the Candida species are frequently isolated contaminants. Elucidating the behavior of a contaminant during the fermentation cycle is essential for combatting the contamination. Consequently, the aim of the current study was to better understand the functional and transcriptional behavior of a contaminating yeast Candida tropicalis. We used a global RNA sequencing approach (RNA-seq/MiSeq) to analyze gene expression. Genes with significantly repressed or induced expression, and related to the fermentations process, such as sugar transport, pyruvate decarboxylase, amino acid metabolism, membrane, tolerance to high concentrations of ethanol and temperatures, nutrient suppression), and transcription-linked processes, were identified. The expression pattern suggested that the functional and transcriptional behavior of the contaminating yeast during fermentation for bioethanol production is similar to that of the standard yeast Saccharomyces cerevisiae. In addition, the analysis confirmed that C. tropicalis is an important contaminant of the alcoholic fermentation process, generating bioethanol and viability through its tolerance to all the adversities of a fermentation process essential for the production of bioethanol. According on the gene expression profile, many of these mechanisms are similar to those of S. cerevisiae strains currently used for bioethanol production. These mechanisms can inform studies on antimicrobials, to combat yeast contamination during industrial bioethanol production.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)São Paulo State University (UNESP) School of Pharmaceutical SciencesSchool of Agromonic Sciences São Paulo State University (UNESP)School of Medicine Science Lisbon UniversitySão Paulo State University (UNESP) School of Pharmaceutical SciencesSchool of Agromonic Sciences São Paulo State University (UNESP)CNPq: 870026/2002-0Universidade Estadual Paulista (Unesp)Lisbon UniversityLourencetti, Natália Manuela Strohmayer [UNESP]Wolf, Ivan Rodrigo [UNESP]Lacerda, Maria Priscila Franco [UNESP]Valente, Guilherme Targino [UNESP]Zanelli, Cleslei Fernando [UNESP]Santoni, Mariana Marchi [UNESP]Mendes-Giannini, Maria José Soares [UNESP]Enguita, Francisco JavierFusco-Almeida, Ana Marisa [UNESP]2019-10-06T16:52:25Z2019-10-06T16:52:25Z2018-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1186/s13568-018-0693-1AMB Express, v. 8, n. 1, 2018.2191-0855http://hdl.handle.net/11449/18979510.1186/s13568-018-0693-12-s2.0-8505461983015256654089001950000-0001-7831-1149Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengAMB Expressinfo:eu-repo/semantics/openAccess2022-02-11T00:17:31Zoai:repositorio.unesp.br:11449/189795Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462022-02-11T00:17:31Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Transcriptional profile of a bioethanol production contaminant Candida tropicalis |
title |
Transcriptional profile of a bioethanol production contaminant Candida tropicalis |
spellingShingle |
Transcriptional profile of a bioethanol production contaminant Candida tropicalis Lourencetti, Natália Manuela Strohmayer [UNESP] Alcoholic fermentation Bioethanol Candida tropicalis Contaminant RNA-seq |
title_short |
Transcriptional profile of a bioethanol production contaminant Candida tropicalis |
title_full |
Transcriptional profile of a bioethanol production contaminant Candida tropicalis |
title_fullStr |
Transcriptional profile of a bioethanol production contaminant Candida tropicalis |
title_full_unstemmed |
Transcriptional profile of a bioethanol production contaminant Candida tropicalis |
title_sort |
Transcriptional profile of a bioethanol production contaminant Candida tropicalis |
author |
Lourencetti, Natália Manuela Strohmayer [UNESP] |
author_facet |
Lourencetti, Natália Manuela Strohmayer [UNESP] Wolf, Ivan Rodrigo [UNESP] Lacerda, Maria Priscila Franco [UNESP] Valente, Guilherme Targino [UNESP] Zanelli, Cleslei Fernando [UNESP] Santoni, Mariana Marchi [UNESP] Mendes-Giannini, Maria José Soares [UNESP] Enguita, Francisco Javier Fusco-Almeida, Ana Marisa [UNESP] |
author_role |
author |
author2 |
Wolf, Ivan Rodrigo [UNESP] Lacerda, Maria Priscila Franco [UNESP] Valente, Guilherme Targino [UNESP] Zanelli, Cleslei Fernando [UNESP] Santoni, Mariana Marchi [UNESP] Mendes-Giannini, Maria José Soares [UNESP] Enguita, Francisco Javier Fusco-Almeida, Ana Marisa [UNESP] |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Lisbon University |
dc.contributor.author.fl_str_mv |
Lourencetti, Natália Manuela Strohmayer [UNESP] Wolf, Ivan Rodrigo [UNESP] Lacerda, Maria Priscila Franco [UNESP] Valente, Guilherme Targino [UNESP] Zanelli, Cleslei Fernando [UNESP] Santoni, Mariana Marchi [UNESP] Mendes-Giannini, Maria José Soares [UNESP] Enguita, Francisco Javier Fusco-Almeida, Ana Marisa [UNESP] |
dc.subject.por.fl_str_mv |
Alcoholic fermentation Bioethanol Candida tropicalis Contaminant RNA-seq |
topic |
Alcoholic fermentation Bioethanol Candida tropicalis Contaminant RNA-seq |
description |
The fermentation process is widely used in the industry for bioethanol production. Even though it is widely used, microbial contamination is unpredictable and difficult to control. The problem of reduced productivity is directly linked to competition for nutrients during contamination. Yeasts representing the Candida species are frequently isolated contaminants. Elucidating the behavior of a contaminant during the fermentation cycle is essential for combatting the contamination. Consequently, the aim of the current study was to better understand the functional and transcriptional behavior of a contaminating yeast Candida tropicalis. We used a global RNA sequencing approach (RNA-seq/MiSeq) to analyze gene expression. Genes with significantly repressed or induced expression, and related to the fermentations process, such as sugar transport, pyruvate decarboxylase, amino acid metabolism, membrane, tolerance to high concentrations of ethanol and temperatures, nutrient suppression), and transcription-linked processes, were identified. The expression pattern suggested that the functional and transcriptional behavior of the contaminating yeast during fermentation for bioethanol production is similar to that of the standard yeast Saccharomyces cerevisiae. In addition, the analysis confirmed that C. tropicalis is an important contaminant of the alcoholic fermentation process, generating bioethanol and viability through its tolerance to all the adversities of a fermentation process essential for the production of bioethanol. According on the gene expression profile, many of these mechanisms are similar to those of S. cerevisiae strains currently used for bioethanol production. These mechanisms can inform studies on antimicrobials, to combat yeast contamination during industrial bioethanol production. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-12-01 2019-10-06T16:52:25Z 2019-10-06T16:52:25Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1186/s13568-018-0693-1 AMB Express, v. 8, n. 1, 2018. 2191-0855 http://hdl.handle.net/11449/189795 10.1186/s13568-018-0693-1 2-s2.0-85054619830 1525665408900195 0000-0001-7831-1149 |
url |
http://dx.doi.org/10.1186/s13568-018-0693-1 http://hdl.handle.net/11449/189795 |
identifier_str_mv |
AMB Express, v. 8, n. 1, 2018. 2191-0855 10.1186/s13568-018-0693-1 2-s2.0-85054619830 1525665408900195 0000-0001-7831-1149 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
AMB Express |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1797790287387426816 |