Effect of Chloroaluminium phthalocyanine in cationic nanoemulsion on photoinactivation of multispecies biofilm
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.pdpdt.2018.10.005 http://hdl.handle.net/11449/189802 |
Resumo: | Background: Photosensitizers in nanocarriers have been investigated for antimicrobial Photodynamic Therapy (aPDT). However, most studies are focused against microorganisms in planktonic or monospecies biofilm. Thus, this in vitro study evaluated the effect of aPDT using Chloroaluminium phthalocyanine (ClAlPc) in cationic nanoemulsion (NE) against Candida albicans, Candida glabrata and Streptococcus mutans grown as multispecies biofilm. Methods: Standard suspensions of each microorganism were added into wells of a microtiter plate for biofilm growth for 48 h in a candle jar. The biofilms were incubated with ClAlPc in cationic NE at 31.8 μM for 30 min and illuminated with red light fluence of 39.3 J/cm2 (P+L+ group). Additional samples were treated only with photosensitizer (P+L-) or red light (P-L+) or neither (P-L-, control group). aPDT efficacy was assessed by colony quantification, biofilm's metabolic activity, total biomass, and confocal microscopy. Data were analyzed by ANOVA/Welch and post-hoc Tukey/Games-Howell tests (α = 0.05). Results: aPDT (P+L+) reduced the colony count in 1.30 to 2.24 lg10 and the metabolic activity in 53.7% compared with the control group (P-L-). The total biomass showed no statistical difference among the groups. The confocal microscopy analyzes showed uptake of the PS in the biofilm, and dead cells were observed in the biofilm treated with aPDT. Conclusion: aPDT mediated by ClAlPc in cationic NE promoted photoinactivation of the multispecies biofilm, which was confirmed by colony quantification, metabolic activity, and confocal microscopy. However, the total biomass of the biofilm was not affected by the treatment. |
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Repositório Institucional da UNESP |
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Effect of Chloroaluminium phthalocyanine in cationic nanoemulsion on photoinactivation of multispecies biofilmAntimicrobial photodynamic therapyBacteriaBiofilmFungiNanocarriersPhotochemotherapyBackground: Photosensitizers in nanocarriers have been investigated for antimicrobial Photodynamic Therapy (aPDT). However, most studies are focused against microorganisms in planktonic or monospecies biofilm. Thus, this in vitro study evaluated the effect of aPDT using Chloroaluminium phthalocyanine (ClAlPc) in cationic nanoemulsion (NE) against Candida albicans, Candida glabrata and Streptococcus mutans grown as multispecies biofilm. Methods: Standard suspensions of each microorganism were added into wells of a microtiter plate for biofilm growth for 48 h in a candle jar. The biofilms were incubated with ClAlPc in cationic NE at 31.8 μM for 30 min and illuminated with red light fluence of 39.3 J/cm2 (P+L+ group). Additional samples were treated only with photosensitizer (P+L-) or red light (P-L+) or neither (P-L-, control group). aPDT efficacy was assessed by colony quantification, biofilm's metabolic activity, total biomass, and confocal microscopy. Data were analyzed by ANOVA/Welch and post-hoc Tukey/Games-Howell tests (α = 0.05). Results: aPDT (P+L+) reduced the colony count in 1.30 to 2.24 lg10 and the metabolic activity in 53.7% compared with the control group (P-L-). The total biomass showed no statistical difference among the groups. The confocal microscopy analyzes showed uptake of the PS in the biofilm, and dead cells were observed in the biofilm treated with aPDT. Conclusion: aPDT mediated by ClAlPc in cationic NE promoted photoinactivation of the multispecies biofilm, which was confirmed by colony quantification, metabolic activity, and confocal microscopy. However, the total biomass of the biofilm was not affected by the treatment.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of Dental Materials and Prosthodontics São Paulo State University (UNESP) School of DentistryCenter of Nanotechnology and Tissue Engineers Photobiology and Photomedicine Research Group FFCLRP—São Paulo UniversityDepartment of Dental Materials and Prosthodontics São Paulo State University (UNESP) School of DentistryFAPESP: 2013/07276-1Universidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Trigo-Gutierrez, Jeffersson Krishan [UNESP]Sanitá, Paula Volpato [UNESP]Tedesco, Antonio ClaudioPavarina, Ana Cláudia [UNESP]Mima, Ewerton Garcia de Oliveira [UNESP]2019-10-06T16:52:39Z2019-10-06T16:52:39Z2018-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article212-219http://dx.doi.org/10.1016/j.pdpdt.2018.10.005Photodiagnosis and Photodynamic Therapy, v. 24, p. 212-219.1873-15971572-1000http://hdl.handle.net/11449/18980210.1016/j.pdpdt.2018.10.0052-s2.0-8505484881462599858990694980000-0002-9575-7625Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPhotodiagnosis and Photodynamic Therapyinfo:eu-repo/semantics/openAccess2021-10-22T20:49:13Zoai:repositorio.unesp.br:11449/189802Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-22T20:49:13Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Effect of Chloroaluminium phthalocyanine in cationic nanoemulsion on photoinactivation of multispecies biofilm |
title |
Effect of Chloroaluminium phthalocyanine in cationic nanoemulsion on photoinactivation of multispecies biofilm |
spellingShingle |
Effect of Chloroaluminium phthalocyanine in cationic nanoemulsion on photoinactivation of multispecies biofilm Trigo-Gutierrez, Jeffersson Krishan [UNESP] Antimicrobial photodynamic therapy Bacteria Biofilm Fungi Nanocarriers Photochemotherapy |
title_short |
Effect of Chloroaluminium phthalocyanine in cationic nanoemulsion on photoinactivation of multispecies biofilm |
title_full |
Effect of Chloroaluminium phthalocyanine in cationic nanoemulsion on photoinactivation of multispecies biofilm |
title_fullStr |
Effect of Chloroaluminium phthalocyanine in cationic nanoemulsion on photoinactivation of multispecies biofilm |
title_full_unstemmed |
Effect of Chloroaluminium phthalocyanine in cationic nanoemulsion on photoinactivation of multispecies biofilm |
title_sort |
Effect of Chloroaluminium phthalocyanine in cationic nanoemulsion on photoinactivation of multispecies biofilm |
author |
Trigo-Gutierrez, Jeffersson Krishan [UNESP] |
author_facet |
Trigo-Gutierrez, Jeffersson Krishan [UNESP] Sanitá, Paula Volpato [UNESP] Tedesco, Antonio Claudio Pavarina, Ana Cláudia [UNESP] Mima, Ewerton Garcia de Oliveira [UNESP] |
author_role |
author |
author2 |
Sanitá, Paula Volpato [UNESP] Tedesco, Antonio Claudio Pavarina, Ana Cláudia [UNESP] Mima, Ewerton Garcia de Oliveira [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Trigo-Gutierrez, Jeffersson Krishan [UNESP] Sanitá, Paula Volpato [UNESP] Tedesco, Antonio Claudio Pavarina, Ana Cláudia [UNESP] Mima, Ewerton Garcia de Oliveira [UNESP] |
dc.subject.por.fl_str_mv |
Antimicrobial photodynamic therapy Bacteria Biofilm Fungi Nanocarriers Photochemotherapy |
topic |
Antimicrobial photodynamic therapy Bacteria Biofilm Fungi Nanocarriers Photochemotherapy |
description |
Background: Photosensitizers in nanocarriers have been investigated for antimicrobial Photodynamic Therapy (aPDT). However, most studies are focused against microorganisms in planktonic or monospecies biofilm. Thus, this in vitro study evaluated the effect of aPDT using Chloroaluminium phthalocyanine (ClAlPc) in cationic nanoemulsion (NE) against Candida albicans, Candida glabrata and Streptococcus mutans grown as multispecies biofilm. Methods: Standard suspensions of each microorganism were added into wells of a microtiter plate for biofilm growth for 48 h in a candle jar. The biofilms were incubated with ClAlPc in cationic NE at 31.8 μM for 30 min and illuminated with red light fluence of 39.3 J/cm2 (P+L+ group). Additional samples were treated only with photosensitizer (P+L-) or red light (P-L+) or neither (P-L-, control group). aPDT efficacy was assessed by colony quantification, biofilm's metabolic activity, total biomass, and confocal microscopy. Data were analyzed by ANOVA/Welch and post-hoc Tukey/Games-Howell tests (α = 0.05). Results: aPDT (P+L+) reduced the colony count in 1.30 to 2.24 lg10 and the metabolic activity in 53.7% compared with the control group (P-L-). The total biomass showed no statistical difference among the groups. The confocal microscopy analyzes showed uptake of the PS in the biofilm, and dead cells were observed in the biofilm treated with aPDT. Conclusion: aPDT mediated by ClAlPc in cationic NE promoted photoinactivation of the multispecies biofilm, which was confirmed by colony quantification, metabolic activity, and confocal microscopy. However, the total biomass of the biofilm was not affected by the treatment. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-12-01 2019-10-06T16:52:39Z 2019-10-06T16:52:39Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.pdpdt.2018.10.005 Photodiagnosis and Photodynamic Therapy, v. 24, p. 212-219. 1873-1597 1572-1000 http://hdl.handle.net/11449/189802 10.1016/j.pdpdt.2018.10.005 2-s2.0-85054848814 6259985899069498 0000-0002-9575-7625 |
url |
http://dx.doi.org/10.1016/j.pdpdt.2018.10.005 http://hdl.handle.net/11449/189802 |
identifier_str_mv |
Photodiagnosis and Photodynamic Therapy, v. 24, p. 212-219. 1873-1597 1572-1000 10.1016/j.pdpdt.2018.10.005 2-s2.0-85054848814 6259985899069498 0000-0002-9575-7625 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Photodiagnosis and Photodynamic Therapy |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
212-219 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1792961970956140544 |