Identification of Plasmodium spp. in Neotropical primates of Maranhense Amazon in Northeast Brazil
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1371/journal.pone.0182905 http://hdl.handle.net/11449/175031 |
Resumo: | In the Brazilian Amazon region, malaria caused by Plasmodium malariae is considered to be a zoonosis because of cross-transfer of the parasite between humans and Neotropical primates. To contribute information on this issue, we investigated occurrences of natural infection with Plasmodium sp. among Neotropical primates in the Maranhense Amazon (Amazon region of the state of Maranhão), in the northeastern region of Brazil. Blood samples were collected from 161 Neotropical primates of six species that were caught in an environmental reserve (Sítio Aguahy) and from captive primates (CETAS—Wildlife Screening Center, municipality of São Luís), in Maranhão. Plasmodium sp. was diagnosed based on light microscopy, PCR, qPCR and LAMP for amplification of the 18S rRNA gene. Serum samples were also assayed by means of indirect immunofluorescence for IgG antibodies against P. malariae/P. brasilianum, P. falciparum and P. berghei. Parasites were detected through light microscopy on five slides from captive primates (four Sapajus spp. and one Callithrix jacchus). In the molecular tests, 34.16% (55/161) and 29.81% (48/161) of the animals sampled were positive in the qPCR and PCR assays, respectively. In the PCR, 47/48 animals were positive for P. malariae/P. brasilianum; of these, eight were free-living primates and 39 from CETAS, São Luís. One sample showed a band in the genus-specific reaction, but not in the second PCR reaction. Anti-P. malariae/P. brasilianum IgG antibodies were detected in four serum samples from Sapajus spp. in captivity. In this study, circulation of P. malariae/P. brasilianum in Neotropical primates was confirmed, with low levels of parasitemia and low levels of antibodies. The importance of these animals as reservoirs of human malaria in the region studied is still unknown. This scenario has an impact on control and elimination of malaria in this region. |
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Identification of Plasmodium spp. in Neotropical primates of Maranhense Amazon in Northeast BrazilIn the Brazilian Amazon region, malaria caused by Plasmodium malariae is considered to be a zoonosis because of cross-transfer of the parasite between humans and Neotropical primates. To contribute information on this issue, we investigated occurrences of natural infection with Plasmodium sp. among Neotropical primates in the Maranhense Amazon (Amazon region of the state of Maranhão), in the northeastern region of Brazil. Blood samples were collected from 161 Neotropical primates of six species that were caught in an environmental reserve (Sítio Aguahy) and from captive primates (CETAS—Wildlife Screening Center, municipality of São Luís), in Maranhão. Plasmodium sp. was diagnosed based on light microscopy, PCR, qPCR and LAMP for amplification of the 18S rRNA gene. Serum samples were also assayed by means of indirect immunofluorescence for IgG antibodies against P. malariae/P. brasilianum, P. falciparum and P. berghei. Parasites were detected through light microscopy on five slides from captive primates (four Sapajus spp. and one Callithrix jacchus). In the molecular tests, 34.16% (55/161) and 29.81% (48/161) of the animals sampled were positive in the qPCR and PCR assays, respectively. In the PCR, 47/48 animals were positive for P. malariae/P. brasilianum; of these, eight were free-living primates and 39 from CETAS, São Luís. One sample showed a band in the genus-specific reaction, but not in the second PCR reaction. Anti-P. malariae/P. brasilianum IgG antibodies were detected in four serum samples from Sapajus spp. in captivity. In this study, circulation of P. malariae/P. brasilianum in Neotropical primates was confirmed, with low levels of parasitemia and low levels of antibodies. The importance of these animals as reservoirs of human malaria in the region studied is still unknown. This scenario has an impact on control and elimination of malaria in this region.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Laboratory of Immunoparasitology School of Agrarian and Veterinary Sciences (FCAV) UNESP Jaboticabal CampusCenter for Malaria Studies Superintendency for Endemic Disease Control (SUCEN) Department of Health of the State of São Paulo Institute of Tropical Medicine of Sao Paulo (IMTSP) USPLaboratory of Veterinary Pathology Brazil University Descalvado CampusLaboratory of Immunoparasitology School of Agrarian and Veterinary Sciences (FCAV) UNESP Jaboticabal CampusFAPESP: 10/12820-4FAPESP: 12/03961-9Universidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Descalvado CampusFigueiredo, Mayra Araguaia Pereira [UNESP]Di Santi, Silvia MariaManrique, Wilson GómezAndré, Marcos Rogério [UNESP]Machado, Rosangela Zacarias [UNESP]2018-12-11T17:13:55Z2018-12-11T17:13:55Z2017-08-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.1371/journal.pone.0182905PLoS ONE, v. 12, n. 8, 2017.1932-6203http://hdl.handle.net/11449/17503110.1371/journal.pone.01829052-s2.0-850272451242-s2.0-85027245124.pdf9139899895580513Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPLoS ONE1,164info:eu-repo/semantics/openAccess2023-10-26T06:06:12Zoai:repositorio.unesp.br:11449/175031Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-10-26T06:06:12Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Identification of Plasmodium spp. in Neotropical primates of Maranhense Amazon in Northeast Brazil |
title |
Identification of Plasmodium spp. in Neotropical primates of Maranhense Amazon in Northeast Brazil |
spellingShingle |
Identification of Plasmodium spp. in Neotropical primates of Maranhense Amazon in Northeast Brazil Figueiredo, Mayra Araguaia Pereira [UNESP] |
title_short |
Identification of Plasmodium spp. in Neotropical primates of Maranhense Amazon in Northeast Brazil |
title_full |
Identification of Plasmodium spp. in Neotropical primates of Maranhense Amazon in Northeast Brazil |
title_fullStr |
Identification of Plasmodium spp. in Neotropical primates of Maranhense Amazon in Northeast Brazil |
title_full_unstemmed |
Identification of Plasmodium spp. in Neotropical primates of Maranhense Amazon in Northeast Brazil |
title_sort |
Identification of Plasmodium spp. in Neotropical primates of Maranhense Amazon in Northeast Brazil |
author |
Figueiredo, Mayra Araguaia Pereira [UNESP] |
author_facet |
Figueiredo, Mayra Araguaia Pereira [UNESP] Di Santi, Silvia Maria Manrique, Wilson Gómez André, Marcos Rogério [UNESP] Machado, Rosangela Zacarias [UNESP] |
author_role |
author |
author2 |
Di Santi, Silvia Maria Manrique, Wilson Gómez André, Marcos Rogério [UNESP] Machado, Rosangela Zacarias [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade de São Paulo (USP) Descalvado Campus |
dc.contributor.author.fl_str_mv |
Figueiredo, Mayra Araguaia Pereira [UNESP] Di Santi, Silvia Maria Manrique, Wilson Gómez André, Marcos Rogério [UNESP] Machado, Rosangela Zacarias [UNESP] |
description |
In the Brazilian Amazon region, malaria caused by Plasmodium malariae is considered to be a zoonosis because of cross-transfer of the parasite between humans and Neotropical primates. To contribute information on this issue, we investigated occurrences of natural infection with Plasmodium sp. among Neotropical primates in the Maranhense Amazon (Amazon region of the state of Maranhão), in the northeastern region of Brazil. Blood samples were collected from 161 Neotropical primates of six species that were caught in an environmental reserve (Sítio Aguahy) and from captive primates (CETAS—Wildlife Screening Center, municipality of São Luís), in Maranhão. Plasmodium sp. was diagnosed based on light microscopy, PCR, qPCR and LAMP for amplification of the 18S rRNA gene. Serum samples were also assayed by means of indirect immunofluorescence for IgG antibodies against P. malariae/P. brasilianum, P. falciparum and P. berghei. Parasites were detected through light microscopy on five slides from captive primates (four Sapajus spp. and one Callithrix jacchus). In the molecular tests, 34.16% (55/161) and 29.81% (48/161) of the animals sampled were positive in the qPCR and PCR assays, respectively. In the PCR, 47/48 animals were positive for P. malariae/P. brasilianum; of these, eight were free-living primates and 39 from CETAS, São Luís. One sample showed a band in the genus-specific reaction, but not in the second PCR reaction. Anti-P. malariae/P. brasilianum IgG antibodies were detected in four serum samples from Sapajus spp. in captivity. In this study, circulation of P. malariae/P. brasilianum in Neotropical primates was confirmed, with low levels of parasitemia and low levels of antibodies. The importance of these animals as reservoirs of human malaria in the region studied is still unknown. This scenario has an impact on control and elimination of malaria in this region. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-08-01 2018-12-11T17:13:55Z 2018-12-11T17:13:55Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1371/journal.pone.0182905 PLoS ONE, v. 12, n. 8, 2017. 1932-6203 http://hdl.handle.net/11449/175031 10.1371/journal.pone.0182905 2-s2.0-85027245124 2-s2.0-85027245124.pdf 9139899895580513 |
url |
http://dx.doi.org/10.1371/journal.pone.0182905 http://hdl.handle.net/11449/175031 |
identifier_str_mv |
PLoS ONE, v. 12, n. 8, 2017. 1932-6203 10.1371/journal.pone.0182905 2-s2.0-85027245124 2-s2.0-85027245124.pdf 9139899895580513 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
PLoS ONE 1,164 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
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Universidade Estadual Paulista (UNESP) |
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Repositório Institucional da UNESP |
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Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
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