All-triploid offspring in the yellowtail tetra Astyanax altiparanae Garutti & Britski 2000 (Teleostei, Characidae) derived from female tetraploid × male diploid crosses
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1017/S0967199422000569 http://hdl.handle.net/11449/249052 |
Resumo: | This study aimed to evaluate the ploidy and survival of larvae resulting from crosses between tetraploid females and diploid males of yellowtail tetra Astyanax altiparanae, both females (three diploids and three tetraploids) and males (n = 3 diploids). Breeders were subjected to hormonal induction with pituitary gland extract from common carp fish (Cyprinus carpio). Females received two doses at concentrations of 0.3 and 3.0 mg/kg -1 body weight and at intervals of 6 h. Males were induced with a single dose of 3.0 mg/kg -1 applied simultaneously with the second dose in females. Oocytes from each diploid and tetraploid female were fertilized with semen from the same male, resulting in two crosses: cross 1 (diploid male and diploid female) and cross 2 (diploid male and tetraploid female). The procedures were performed with separate females (diploid and tetraploid) and diploid males for each repetition (n = 3). For ploidy determination, 60 larvae from each treatment were analyzed using flow cytometry and cytogenetic analyses. As expected, flow cytometry analysis showed that progenies from crosses 1 and 2 presented diploid and triploid individuals, respectively, with a 100% success rate. The same results were confirmed in the cytogenetic analysis, in which the larvae resulting from cross 1 had 50 metaphase chromosomes and those from cross 2 had 75 chromosomes. The oocytes have a slightly ovoid shape at the time of extrusion. Diploid oocytes had a size of 559 ± 20.62 μm and tetraploid of 1025.33 ± 30.91 μm. Statistical differences were observed between eggs from crosses 1 and 2 (P = 0.0130). No significant differences between treatments were observed for survival at the 2-cell stage (P = 0.6174), blastula (P = 0.9717), gastrula (P = 0.5301), somite (P = 0.3811), and hatching (P = 0.0984) stages. In conclusion, our results showed that tetraploid females of the yellowtail tetra A. altiparanae are fertile, present viable gametes after stripping and fertilization using the 'dry method', and may be used for mass production of triploids. This is the first report of these procedures within neotropical characins, and which can be applied in other related species of economic importance. |
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All-triploid offspring in the yellowtail tetra Astyanax altiparanae Garutti & Britski 2000 (Teleostei, Characidae) derived from female tetraploid × male diploid crossesAquacultureChromosome manipulationEmbryologyLambariTriploids mass productionThis study aimed to evaluate the ploidy and survival of larvae resulting from crosses between tetraploid females and diploid males of yellowtail tetra Astyanax altiparanae, both females (three diploids and three tetraploids) and males (n = 3 diploids). Breeders were subjected to hormonal induction with pituitary gland extract from common carp fish (Cyprinus carpio). Females received two doses at concentrations of 0.3 and 3.0 mg/kg -1 body weight and at intervals of 6 h. Males were induced with a single dose of 3.0 mg/kg -1 applied simultaneously with the second dose in females. Oocytes from each diploid and tetraploid female were fertilized with semen from the same male, resulting in two crosses: cross 1 (diploid male and diploid female) and cross 2 (diploid male and tetraploid female). The procedures were performed with separate females (diploid and tetraploid) and diploid males for each repetition (n = 3). For ploidy determination, 60 larvae from each treatment were analyzed using flow cytometry and cytogenetic analyses. As expected, flow cytometry analysis showed that progenies from crosses 1 and 2 presented diploid and triploid individuals, respectively, with a 100% success rate. The same results were confirmed in the cytogenetic analysis, in which the larvae resulting from cross 1 had 50 metaphase chromosomes and those from cross 2 had 75 chromosomes. The oocytes have a slightly ovoid shape at the time of extrusion. Diploid oocytes had a size of 559 ± 20.62 μm and tetraploid of 1025.33 ± 30.91 μm. Statistical differences were observed between eggs from crosses 1 and 2 (P = 0.0130). No significant differences between treatments were observed for survival at the 2-cell stage (P = 0.6174), blastula (P = 0.9717), gastrula (P = 0.5301), somite (P = 0.3811), and hatching (P = 0.0984) stages. In conclusion, our results showed that tetraploid females of the yellowtail tetra A. altiparanae are fertile, present viable gametes after stripping and fertilization using the 'dry method', and may be used for mass production of triploids. This is the first report of these procedures within neotropical characins, and which can be applied in other related species of economic importance.Federal Rural University of Rio de Janeiro (UFRRJ). Animal Science Graduate Programme, Km 7 ,Zona Rurals/nLaboratory of Fish Biotechnology Centro Nacional de Pesquisa e Conservação da Biodiversidade Aquática ContinentalTA/ICMbio, Rodovia Pref. Euberto Nemésio Pereira de GodoyUniversity of São Paulo School of Veterinary Medicine and Animal Science Department of Animal ReproductionFederal Rural University of Pernambuco (UFRPE) Unidade Acadêmica de Serra Talhada, s/nPaulista State University (UNESP) Department of ZoologyPaulista State University (UNESP) Department of ZoologyFederal Rural University of Rio de Janeiro (UFRRJ). Animal Science Graduate ProgrammeCentro Nacional de Pesquisa e Conservação da Biodiversidade Aquática ContinentalTA/ICMbioUniversidade de São Paulo (USP)Unidade Acadêmica de Serra TalhadaUniversidade Estadual Paulista (UNESP)Alves, Andreoli CorreiaYasui, George Shiguekido Nascimento, Nivaldo FerreiraMonzani, Paulo Sérgio [UNESP]Senhorini, José Augusto [UNESP]Pereira Dos Santos, Matheus2023-07-29T14:01:05Z2023-07-29T14:01:05Z2023-04-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article123-128http://dx.doi.org/10.1017/S0967199422000569Zygote (Cambridge, England), v. 31, n. 2, p. 123-128, 2023.1469-8730http://hdl.handle.net/11449/24905210.1017/S09671994220005692-s2.0-85150000760Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengZygote (Cambridge, England)info:eu-repo/semantics/openAccess2023-07-29T14:01:05Zoai:repositorio.unesp.br:11449/249052Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-07-29T14:01:05Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
All-triploid offspring in the yellowtail tetra Astyanax altiparanae Garutti & Britski 2000 (Teleostei, Characidae) derived from female tetraploid × male diploid crosses |
title |
All-triploid offspring in the yellowtail tetra Astyanax altiparanae Garutti & Britski 2000 (Teleostei, Characidae) derived from female tetraploid × male diploid crosses |
spellingShingle |
All-triploid offspring in the yellowtail tetra Astyanax altiparanae Garutti & Britski 2000 (Teleostei, Characidae) derived from female tetraploid × male diploid crosses Alves, Andreoli Correia Aquaculture Chromosome manipulation Embryology Lambari Triploids mass production |
title_short |
All-triploid offspring in the yellowtail tetra Astyanax altiparanae Garutti & Britski 2000 (Teleostei, Characidae) derived from female tetraploid × male diploid crosses |
title_full |
All-triploid offspring in the yellowtail tetra Astyanax altiparanae Garutti & Britski 2000 (Teleostei, Characidae) derived from female tetraploid × male diploid crosses |
title_fullStr |
All-triploid offspring in the yellowtail tetra Astyanax altiparanae Garutti & Britski 2000 (Teleostei, Characidae) derived from female tetraploid × male diploid crosses |
title_full_unstemmed |
All-triploid offspring in the yellowtail tetra Astyanax altiparanae Garutti & Britski 2000 (Teleostei, Characidae) derived from female tetraploid × male diploid crosses |
title_sort |
All-triploid offspring in the yellowtail tetra Astyanax altiparanae Garutti & Britski 2000 (Teleostei, Characidae) derived from female tetraploid × male diploid crosses |
author |
Alves, Andreoli Correia |
author_facet |
Alves, Andreoli Correia Yasui, George Shigueki do Nascimento, Nivaldo Ferreira Monzani, Paulo Sérgio [UNESP] Senhorini, José Augusto [UNESP] Pereira Dos Santos, Matheus |
author_role |
author |
author2 |
Yasui, George Shigueki do Nascimento, Nivaldo Ferreira Monzani, Paulo Sérgio [UNESP] Senhorini, José Augusto [UNESP] Pereira Dos Santos, Matheus |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Federal Rural University of Rio de Janeiro (UFRRJ). Animal Science Graduate Programme Centro Nacional de Pesquisa e Conservação da Biodiversidade Aquática ContinentalTA/ICMbio Universidade de São Paulo (USP) Unidade Acadêmica de Serra Talhada Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Alves, Andreoli Correia Yasui, George Shigueki do Nascimento, Nivaldo Ferreira Monzani, Paulo Sérgio [UNESP] Senhorini, José Augusto [UNESP] Pereira Dos Santos, Matheus |
dc.subject.por.fl_str_mv |
Aquaculture Chromosome manipulation Embryology Lambari Triploids mass production |
topic |
Aquaculture Chromosome manipulation Embryology Lambari Triploids mass production |
description |
This study aimed to evaluate the ploidy and survival of larvae resulting from crosses between tetraploid females and diploid males of yellowtail tetra Astyanax altiparanae, both females (three diploids and three tetraploids) and males (n = 3 diploids). Breeders were subjected to hormonal induction with pituitary gland extract from common carp fish (Cyprinus carpio). Females received two doses at concentrations of 0.3 and 3.0 mg/kg -1 body weight and at intervals of 6 h. Males were induced with a single dose of 3.0 mg/kg -1 applied simultaneously with the second dose in females. Oocytes from each diploid and tetraploid female were fertilized with semen from the same male, resulting in two crosses: cross 1 (diploid male and diploid female) and cross 2 (diploid male and tetraploid female). The procedures were performed with separate females (diploid and tetraploid) and diploid males for each repetition (n = 3). For ploidy determination, 60 larvae from each treatment were analyzed using flow cytometry and cytogenetic analyses. As expected, flow cytometry analysis showed that progenies from crosses 1 and 2 presented diploid and triploid individuals, respectively, with a 100% success rate. The same results were confirmed in the cytogenetic analysis, in which the larvae resulting from cross 1 had 50 metaphase chromosomes and those from cross 2 had 75 chromosomes. The oocytes have a slightly ovoid shape at the time of extrusion. Diploid oocytes had a size of 559 ± 20.62 μm and tetraploid of 1025.33 ± 30.91 μm. Statistical differences were observed between eggs from crosses 1 and 2 (P = 0.0130). No significant differences between treatments were observed for survival at the 2-cell stage (P = 0.6174), blastula (P = 0.9717), gastrula (P = 0.5301), somite (P = 0.3811), and hatching (P = 0.0984) stages. In conclusion, our results showed that tetraploid females of the yellowtail tetra A. altiparanae are fertile, present viable gametes after stripping and fertilization using the 'dry method', and may be used for mass production of triploids. This is the first report of these procedures within neotropical characins, and which can be applied in other related species of economic importance. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-07-29T14:01:05Z 2023-07-29T14:01:05Z 2023-04-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1017/S0967199422000569 Zygote (Cambridge, England), v. 31, n. 2, p. 123-128, 2023. 1469-8730 http://hdl.handle.net/11449/249052 10.1017/S0967199422000569 2-s2.0-85150000760 |
url |
http://dx.doi.org/10.1017/S0967199422000569 http://hdl.handle.net/11449/249052 |
identifier_str_mv |
Zygote (Cambridge, England), v. 31, n. 2, p. 123-128, 2023. 1469-8730 10.1017/S0967199422000569 2-s2.0-85150000760 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Zygote (Cambridge, England) |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
123-128 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965003206361088 |