Species level identification of coagulase negative Staphylococcus spp. from buffalo using matrix-assisted laser desorption ionization–time of flight mass spectrometry and cydB real-time quantitative PCR
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.vetmic.2017.03.036 http://hdl.handle.net/11449/174461 |
Resumo: | Incorrect identification of Staphylococcus spp. can have serious clinical and zoonotic repercussions. Accordingly, the aim of this study was to determine if matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) and/or cydB real- time quantitative PCR (qPCR) could be used to accurately identify coagulase negative Staphylococcus spp. (CoNS) obtained from buffalo milk and milking environment samples. Seventy-five of 84 CoNS isolates could be identified to the species level (score value >1.99) using MALDI-TOF MS. However, as determined by cytochrome d ubiquinol oxidase subunit II (cydB) qPCR and by 16S RNA and cydB gene sequencing, 10 S. agnetis strains were wrongly identified as S. hyicus by MALDI-TOF MS. In addition, 9 isolates identified by MALDI-TOF only to the genus level (score values between 1.70 and 1.99) could be identified to species by cydB qPCR. Our findings suggest that MALDI-TOF MS is a reliable method for rapid identification of S. chromogenes and S. epidermidis (species of interest both in human and veterinary medicine) and may be able to correctly identify other Staphylococcus spp. However, at present not all Staphylococcus spp. found in buffalo milk can be accurately identified by MALDI-TOF MS and for these organisms, the cydB qPCR developed in the current study may provide a reliable alternative method for rapid identification of CoNS species. |
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Species level identification of coagulase negative Staphylococcus spp. from buffalo using matrix-assisted laser desorption ionization–time of flight mass spectrometry and cydB real-time quantitative PCRcydB qPCRMALDI-TOF MSMilkStaphylococcus agnetisStaphylococcus hyicusIncorrect identification of Staphylococcus spp. can have serious clinical and zoonotic repercussions. Accordingly, the aim of this study was to determine if matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) and/or cydB real- time quantitative PCR (qPCR) could be used to accurately identify coagulase negative Staphylococcus spp. (CoNS) obtained from buffalo milk and milking environment samples. Seventy-five of 84 CoNS isolates could be identified to the species level (score value >1.99) using MALDI-TOF MS. However, as determined by cytochrome d ubiquinol oxidase subunit II (cydB) qPCR and by 16S RNA and cydB gene sequencing, 10 S. agnetis strains were wrongly identified as S. hyicus by MALDI-TOF MS. In addition, 9 isolates identified by MALDI-TOF only to the genus level (score values between 1.70 and 1.99) could be identified to species by cydB qPCR. Our findings suggest that MALDI-TOF MS is a reliable method for rapid identification of S. chromogenes and S. epidermidis (species of interest both in human and veterinary medicine) and may be able to correctly identify other Staphylococcus spp. However, at present not all Staphylococcus spp. found in buffalo milk can be accurately identified by MALDI-TOF MS and for these organisms, the cydB qPCR developed in the current study may provide a reliable alternative method for rapid identification of CoNS species.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Department of Veterinary Preventive Medicine and Animal Reproduction São Paulo State University (Unesp) School of Agricultural and Veterinarian Sciences, JaboticabalDepartment of Veterinary Pathology São Paulo State University (Unesp) School of Agricultural and Veterinarian Sciences, JaboticabalUniversity of Guelph Department of Pathobiology, 50 Stone Rd. EastAnimal Health Laboratory University of Guelph, Post Office 3612Brazilian Agricultural Research Corporation (EMBRAPA) Embrapa Southeast Livestock, Washington Luiz road, Km 234Washington Luiz road, Km 234Department of Veterinary Preventive Medicine and Animal Reproduction São Paulo State University (Unesp) School of Agricultural and Veterinarian Sciences, JaboticabalDepartment of Veterinary Pathology São Paulo State University (Unesp) School of Agricultural and Veterinarian Sciences, JaboticabalUniversidade Estadual Paulista (Unesp)University of GuelphEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA)Pizauro, Lucas J.L. [UNESP]de Almeida, Camila C. [UNESP]Soltes, Glenn A.Slavic, DurdaRossi-Junior, Oswaldo D. [UNESP]de Ávila, Fernando. A. [UNESP]Zafalon, Luiz. F.MacInnes, Janet I.2018-12-11T17:11:13Z2018-12-11T17:11:13Z2017-05-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article8-14application/pdfhttp://dx.doi.org/10.1016/j.vetmic.2017.03.036Veterinary Microbiology, v. 204, p. 8-14.1873-25420378-1135http://hdl.handle.net/11449/17446110.1016/j.vetmic.2017.03.0362-s2.0-850174291232-s2.0-85017429123.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengVeterinary Microbiology1,175info:eu-repo/semantics/openAccess2023-11-07T06:13:55Zoai:repositorio.unesp.br:11449/174461Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-11-07T06:13:55Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Species level identification of coagulase negative Staphylococcus spp. from buffalo using matrix-assisted laser desorption ionization–time of flight mass spectrometry and cydB real-time quantitative PCR |
title |
Species level identification of coagulase negative Staphylococcus spp. from buffalo using matrix-assisted laser desorption ionization–time of flight mass spectrometry and cydB real-time quantitative PCR |
spellingShingle |
Species level identification of coagulase negative Staphylococcus spp. from buffalo using matrix-assisted laser desorption ionization–time of flight mass spectrometry and cydB real-time quantitative PCR Pizauro, Lucas J.L. [UNESP] cydB qPCR MALDI-TOF MS Milk Staphylococcus agnetis Staphylococcus hyicus |
title_short |
Species level identification of coagulase negative Staphylococcus spp. from buffalo using matrix-assisted laser desorption ionization–time of flight mass spectrometry and cydB real-time quantitative PCR |
title_full |
Species level identification of coagulase negative Staphylococcus spp. from buffalo using matrix-assisted laser desorption ionization–time of flight mass spectrometry and cydB real-time quantitative PCR |
title_fullStr |
Species level identification of coagulase negative Staphylococcus spp. from buffalo using matrix-assisted laser desorption ionization–time of flight mass spectrometry and cydB real-time quantitative PCR |
title_full_unstemmed |
Species level identification of coagulase negative Staphylococcus spp. from buffalo using matrix-assisted laser desorption ionization–time of flight mass spectrometry and cydB real-time quantitative PCR |
title_sort |
Species level identification of coagulase negative Staphylococcus spp. from buffalo using matrix-assisted laser desorption ionization–time of flight mass spectrometry and cydB real-time quantitative PCR |
author |
Pizauro, Lucas J.L. [UNESP] |
author_facet |
Pizauro, Lucas J.L. [UNESP] de Almeida, Camila C. [UNESP] Soltes, Glenn A. Slavic, Durda Rossi-Junior, Oswaldo D. [UNESP] de Ávila, Fernando. A. [UNESP] Zafalon, Luiz. F. MacInnes, Janet I. |
author_role |
author |
author2 |
de Almeida, Camila C. [UNESP] Soltes, Glenn A. Slavic, Durda Rossi-Junior, Oswaldo D. [UNESP] de Ávila, Fernando. A. [UNESP] Zafalon, Luiz. F. MacInnes, Janet I. |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) University of Guelph Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA) |
dc.contributor.author.fl_str_mv |
Pizauro, Lucas J.L. [UNESP] de Almeida, Camila C. [UNESP] Soltes, Glenn A. Slavic, Durda Rossi-Junior, Oswaldo D. [UNESP] de Ávila, Fernando. A. [UNESP] Zafalon, Luiz. F. MacInnes, Janet I. |
dc.subject.por.fl_str_mv |
cydB qPCR MALDI-TOF MS Milk Staphylococcus agnetis Staphylococcus hyicus |
topic |
cydB qPCR MALDI-TOF MS Milk Staphylococcus agnetis Staphylococcus hyicus |
description |
Incorrect identification of Staphylococcus spp. can have serious clinical and zoonotic repercussions. Accordingly, the aim of this study was to determine if matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) and/or cydB real- time quantitative PCR (qPCR) could be used to accurately identify coagulase negative Staphylococcus spp. (CoNS) obtained from buffalo milk and milking environment samples. Seventy-five of 84 CoNS isolates could be identified to the species level (score value >1.99) using MALDI-TOF MS. However, as determined by cytochrome d ubiquinol oxidase subunit II (cydB) qPCR and by 16S RNA and cydB gene sequencing, 10 S. agnetis strains were wrongly identified as S. hyicus by MALDI-TOF MS. In addition, 9 isolates identified by MALDI-TOF only to the genus level (score values between 1.70 and 1.99) could be identified to species by cydB qPCR. Our findings suggest that MALDI-TOF MS is a reliable method for rapid identification of S. chromogenes and S. epidermidis (species of interest both in human and veterinary medicine) and may be able to correctly identify other Staphylococcus spp. However, at present not all Staphylococcus spp. found in buffalo milk can be accurately identified by MALDI-TOF MS and for these organisms, the cydB qPCR developed in the current study may provide a reliable alternative method for rapid identification of CoNS species. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-05-01 2018-12-11T17:11:13Z 2018-12-11T17:11:13Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.vetmic.2017.03.036 Veterinary Microbiology, v. 204, p. 8-14. 1873-2542 0378-1135 http://hdl.handle.net/11449/174461 10.1016/j.vetmic.2017.03.036 2-s2.0-85017429123 2-s2.0-85017429123.pdf |
url |
http://dx.doi.org/10.1016/j.vetmic.2017.03.036 http://hdl.handle.net/11449/174461 |
identifier_str_mv |
Veterinary Microbiology, v. 204, p. 8-14. 1873-2542 0378-1135 10.1016/j.vetmic.2017.03.036 2-s2.0-85017429123 2-s2.0-85017429123.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Veterinary Microbiology 1,175 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
8-14 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799964852400160768 |