Increasing the length of an estradiol with progesterone timed artificial insemination protocol with 2 controlled internal drug release devices improves pregnancy per artificial insemination in lactating dairy cows
Autor(a) principal: | |
---|---|
Data de Publicação: | 2021 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3168/jds.2020-18872 http://hdl.handle.net/11449/205416 |
Resumo: | The objective of this study was to compare the effects of different lengths of ovulation synchronization protocols using 2 controlled internal drug release (CIDR) devices on ovarian dynamics and pregnancy outcomes in lactating dairy cows. Lactating Holstein cows (n = 1,979) were randomly assigned to receive timed artificial insemination (TAI; d 0) following 1 of 2 treatments: (1) 9-d protocol (n = 988; 9D) with 2 intravaginal devices containing 1.9 g of progesterone (CIDR) and 2.0 mg of estradiol benzoate on day −11; 25 mg (i.m.) of dinoprost tromethamine (PG) and withdrawal of 1 CIDR on d −4; 1.0 mg (i.m.) of estradiol cypionate, second CIDR withdrawal, and PG on d −2; and TAI on d 0 and (2) 10-d protocol (n = 991; 10D) with 2 CIDR and 2.0 mg of estradiol benzoate on d −12; 25 mg of PG and withdrawal of 1 CIDR on d −4; 1.0 mg of estradiol, second CIDR withdrawal, and PG on d −2; and TAI on d 0. There was no effect of protocol on estrus detection, whereas a greater percentage of cows from 10D had ovulated close to TAI [no corpus luteum (CL) at AI and a CL at d 7] versus cows assigned to 9D protocol. A protocol × heat stress (average cow temperature ≥39.1°C on day of AI and d 7) interaction was observed in a manner that pregnancy per AI (P/AI) was greater in non-heat-stressed 10D versus 9D cows, whereas P/AI did not differ when cows were under heat stress. Furthermore, 10D protocol did not increase P/AI when all cows that received AI were included in the analysis or in cows that ovulated near TAI. However, animals assigned to 9D without any event of heat stress had a reduced P/AI when compared with cows assigned to 10D without heat stress. A protocol × CL presence at the beginning of the protocol interaction was observed and cows with a CL at the beginning of the protocol had a greater P/AI in 10D versus 9D; meanwhile, in cows without a CL, no differences on P/AI were observed. The protocol × CL presence at the beginning of the protocol interaction on P/AI was also observed for cows that ovulated near TAI. A greater percentage of cows assigned to 9D had follicles of medium size (13–15.9 mm), and greater percentage of cows assigned to 10D had larger follicles (>16 mm). Increasing the length of an estradiol with progesterone–based ovulation synchronization protocol (10D vs. 9D) increased the proportion of cows with larger follicles (>16 mm) and increased P/AI in cows without heat stress and in cows with a CL at beginning of the protocol. Moreover, the 10D protocol increased the proportion of cows with ovulation near TAI, demonstrating the effectiveness of this protocol in improving the reproductive performance of lactating Holstein cows. |
id |
UNSP_ff0f443a0a578392bb6177f55f9f00e5 |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/205416 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Increasing the length of an estradiol with progesterone timed artificial insemination protocol with 2 controlled internal drug release devices improves pregnancy per artificial insemination in lactating dairy cowsartificial inseminationdairy cowsfertilitysynchronizationThe objective of this study was to compare the effects of different lengths of ovulation synchronization protocols using 2 controlled internal drug release (CIDR) devices on ovarian dynamics and pregnancy outcomes in lactating dairy cows. Lactating Holstein cows (n = 1,979) were randomly assigned to receive timed artificial insemination (TAI; d 0) following 1 of 2 treatments: (1) 9-d protocol (n = 988; 9D) with 2 intravaginal devices containing 1.9 g of progesterone (CIDR) and 2.0 mg of estradiol benzoate on day −11; 25 mg (i.m.) of dinoprost tromethamine (PG) and withdrawal of 1 CIDR on d −4; 1.0 mg (i.m.) of estradiol cypionate, second CIDR withdrawal, and PG on d −2; and TAI on d 0 and (2) 10-d protocol (n = 991; 10D) with 2 CIDR and 2.0 mg of estradiol benzoate on d −12; 25 mg of PG and withdrawal of 1 CIDR on d −4; 1.0 mg of estradiol, second CIDR withdrawal, and PG on d −2; and TAI on d 0. There was no effect of protocol on estrus detection, whereas a greater percentage of cows from 10D had ovulated close to TAI [no corpus luteum (CL) at AI and a CL at d 7] versus cows assigned to 9D protocol. A protocol × heat stress (average cow temperature ≥39.1°C on day of AI and d 7) interaction was observed in a manner that pregnancy per AI (P/AI) was greater in non-heat-stressed 10D versus 9D cows, whereas P/AI did not differ when cows were under heat stress. Furthermore, 10D protocol did not increase P/AI when all cows that received AI were included in the analysis or in cows that ovulated near TAI. However, animals assigned to 9D without any event of heat stress had a reduced P/AI when compared with cows assigned to 10D without heat stress. A protocol × CL presence at the beginning of the protocol interaction was observed and cows with a CL at the beginning of the protocol had a greater P/AI in 10D versus 9D; meanwhile, in cows without a CL, no differences on P/AI were observed. The protocol × CL presence at the beginning of the protocol interaction on P/AI was also observed for cows that ovulated near TAI. A greater percentage of cows assigned to 9D had follicles of medium size (13–15.9 mm), and greater percentage of cows assigned to 10D had larger follicles (>16 mm). Increasing the length of an estradiol with progesterone–based ovulation synchronization protocol (10D vs. 9D) increased the proportion of cows with larger follicles (>16 mm) and increased P/AI in cows without heat stress and in cows with a CL at beginning of the protocol. Moreover, the 10D protocol increased the proportion of cows with ovulation near TAI, demonstrating the effectiveness of this protocol in improving the reproductive performance of lactating Holstein cows.Faculdade de Medicina Veterinária e Zootecnia Universidade Estadual Paulista (UNESP)Department of Animal Science Texas A&M UniversityNutricorpDepartment of Animal Production São Paulo State UniversityFaculdade de Medicina Veterinária e Zootecnia Universidade Estadual Paulista (UNESP)Department of Animal Production São Paulo State UniversityUniversidade Estadual Paulista (Unesp)Texas A&M UniversityNutricorpPereira, M. H.C. [UNESP]Lopes, F. R. [UNESP]Munhoz, A. K. [UNESP]Pohler, K. G.Filho, R. V.O.Cappellozza, B. I.Vasconcelos, J. L.M. [UNESP]2021-06-25T10:15:00Z2021-06-25T10:15:00Z2021-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1073-1086http://dx.doi.org/10.3168/jds.2020-18872Journal of Dairy Science, v. 104, n. 1, p. 1073-1086, 2021.1525-31980022-0302http://hdl.handle.net/11449/20541610.3168/jds.2020-188722-s2.0-85094837711Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Dairy Scienceinfo:eu-repo/semantics/openAccess2021-10-23T12:40:05Zoai:repositorio.unesp.br:11449/205416Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T12:40:05Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Increasing the length of an estradiol with progesterone timed artificial insemination protocol with 2 controlled internal drug release devices improves pregnancy per artificial insemination in lactating dairy cows |
title |
Increasing the length of an estradiol with progesterone timed artificial insemination protocol with 2 controlled internal drug release devices improves pregnancy per artificial insemination in lactating dairy cows |
spellingShingle |
Increasing the length of an estradiol with progesterone timed artificial insemination protocol with 2 controlled internal drug release devices improves pregnancy per artificial insemination in lactating dairy cows Pereira, M. H.C. [UNESP] artificial insemination dairy cows fertility synchronization |
title_short |
Increasing the length of an estradiol with progesterone timed artificial insemination protocol with 2 controlled internal drug release devices improves pregnancy per artificial insemination in lactating dairy cows |
title_full |
Increasing the length of an estradiol with progesterone timed artificial insemination protocol with 2 controlled internal drug release devices improves pregnancy per artificial insemination in lactating dairy cows |
title_fullStr |
Increasing the length of an estradiol with progesterone timed artificial insemination protocol with 2 controlled internal drug release devices improves pregnancy per artificial insemination in lactating dairy cows |
title_full_unstemmed |
Increasing the length of an estradiol with progesterone timed artificial insemination protocol with 2 controlled internal drug release devices improves pregnancy per artificial insemination in lactating dairy cows |
title_sort |
Increasing the length of an estradiol with progesterone timed artificial insemination protocol with 2 controlled internal drug release devices improves pregnancy per artificial insemination in lactating dairy cows |
author |
Pereira, M. H.C. [UNESP] |
author_facet |
Pereira, M. H.C. [UNESP] Lopes, F. R. [UNESP] Munhoz, A. K. [UNESP] Pohler, K. G. Filho, R. V.O. Cappellozza, B. I. Vasconcelos, J. L.M. [UNESP] |
author_role |
author |
author2 |
Lopes, F. R. [UNESP] Munhoz, A. K. [UNESP] Pohler, K. G. Filho, R. V.O. Cappellozza, B. I. Vasconcelos, J. L.M. [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Texas A&M University Nutricorp |
dc.contributor.author.fl_str_mv |
Pereira, M. H.C. [UNESP] Lopes, F. R. [UNESP] Munhoz, A. K. [UNESP] Pohler, K. G. Filho, R. V.O. Cappellozza, B. I. Vasconcelos, J. L.M. [UNESP] |
dc.subject.por.fl_str_mv |
artificial insemination dairy cows fertility synchronization |
topic |
artificial insemination dairy cows fertility synchronization |
description |
The objective of this study was to compare the effects of different lengths of ovulation synchronization protocols using 2 controlled internal drug release (CIDR) devices on ovarian dynamics and pregnancy outcomes in lactating dairy cows. Lactating Holstein cows (n = 1,979) were randomly assigned to receive timed artificial insemination (TAI; d 0) following 1 of 2 treatments: (1) 9-d protocol (n = 988; 9D) with 2 intravaginal devices containing 1.9 g of progesterone (CIDR) and 2.0 mg of estradiol benzoate on day −11; 25 mg (i.m.) of dinoprost tromethamine (PG) and withdrawal of 1 CIDR on d −4; 1.0 mg (i.m.) of estradiol cypionate, second CIDR withdrawal, and PG on d −2; and TAI on d 0 and (2) 10-d protocol (n = 991; 10D) with 2 CIDR and 2.0 mg of estradiol benzoate on d −12; 25 mg of PG and withdrawal of 1 CIDR on d −4; 1.0 mg of estradiol, second CIDR withdrawal, and PG on d −2; and TAI on d 0. There was no effect of protocol on estrus detection, whereas a greater percentage of cows from 10D had ovulated close to TAI [no corpus luteum (CL) at AI and a CL at d 7] versus cows assigned to 9D protocol. A protocol × heat stress (average cow temperature ≥39.1°C on day of AI and d 7) interaction was observed in a manner that pregnancy per AI (P/AI) was greater in non-heat-stressed 10D versus 9D cows, whereas P/AI did not differ when cows were under heat stress. Furthermore, 10D protocol did not increase P/AI when all cows that received AI were included in the analysis or in cows that ovulated near TAI. However, animals assigned to 9D without any event of heat stress had a reduced P/AI when compared with cows assigned to 10D without heat stress. A protocol × CL presence at the beginning of the protocol interaction was observed and cows with a CL at the beginning of the protocol had a greater P/AI in 10D versus 9D; meanwhile, in cows without a CL, no differences on P/AI were observed. The protocol × CL presence at the beginning of the protocol interaction on P/AI was also observed for cows that ovulated near TAI. A greater percentage of cows assigned to 9D had follicles of medium size (13–15.9 mm), and greater percentage of cows assigned to 10D had larger follicles (>16 mm). Increasing the length of an estradiol with progesterone–based ovulation synchronization protocol (10D vs. 9D) increased the proportion of cows with larger follicles (>16 mm) and increased P/AI in cows without heat stress and in cows with a CL at beginning of the protocol. Moreover, the 10D protocol increased the proportion of cows with ovulation near TAI, demonstrating the effectiveness of this protocol in improving the reproductive performance of lactating Holstein cows. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-25T10:15:00Z 2021-06-25T10:15:00Z 2021-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3168/jds.2020-18872 Journal of Dairy Science, v. 104, n. 1, p. 1073-1086, 2021. 1525-3198 0022-0302 http://hdl.handle.net/11449/205416 10.3168/jds.2020-18872 2-s2.0-85094837711 |
url |
http://dx.doi.org/10.3168/jds.2020-18872 http://hdl.handle.net/11449/205416 |
identifier_str_mv |
Journal of Dairy Science, v. 104, n. 1, p. 1073-1086, 2021. 1525-3198 0022-0302 10.3168/jds.2020-18872 2-s2.0-85094837711 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Dairy Science |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1073-1086 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1797789862022086656 |