Adenovirus surveillance in wild carnivores from Brazil
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.meegid.2022.105246 http://hdl.handle.net/11449/223468 |
Resumo: | Landscape transformation favors the spread of new pathogens that can be shared between domestic and wild animals. Certain adenoviruses (e.g., canine adenovirus 1 and 2, family Adenoviridae) can infect domestic and wild carnivores. In domestic canids, these viruses are associated with hepatic and respiratory diseases (among others). Nevertheless, information regarding adenovirus pathogenicity and molecular features in wild carnivores is still limited. Herein we surveyed adenovirus in free-ranging carnivores from Brazil. Total DNA was extracted from and subsequently tested by a nested panPCR in spleen and/or lung of 52 carnivores, representing species of the following families: Canidae (n = 4), Felidae (n = 3), Mustelidae (n = 2) and Procyonidae (n = 2). The obtained sequences were compared to others available at GenBank. Available tissue samples from the positive cases were evaluated histopathologically. One out of 52 (1.9%, CI 95%, 0.0–5.7%) carnivores was positive; a roadkilled ocelot (Leopardus pardalis). The obtained sequence presented a low deduced amino acid (78.1%) similarity with the closest adenovirus, identified in a pinniped from the United States of America. This fact and its detection in a novel host suggest it may be representative of a novel species and denominated ocelot adenovirus 1. None of the gross and microscopic findings of the positive case were associated with adenovirus. To the authors' knowledge, this is the first report of adenovirus in wild felids of South America and the second worldwide. Further studies are necessary to assess the epidemiology and potential pathogenicity of this agent in wild carnivores. |
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Adenovirus surveillance in wild carnivores from BrazilLandscape transformationOcelotRoadkillSouth AmericaViral discoveryWildlifeLandscape transformation favors the spread of new pathogens that can be shared between domestic and wild animals. Certain adenoviruses (e.g., canine adenovirus 1 and 2, family Adenoviridae) can infect domestic and wild carnivores. In domestic canids, these viruses are associated with hepatic and respiratory diseases (among others). Nevertheless, information regarding adenovirus pathogenicity and molecular features in wild carnivores is still limited. Herein we surveyed adenovirus in free-ranging carnivores from Brazil. Total DNA was extracted from and subsequently tested by a nested panPCR in spleen and/or lung of 52 carnivores, representing species of the following families: Canidae (n = 4), Felidae (n = 3), Mustelidae (n = 2) and Procyonidae (n = 2). The obtained sequences were compared to others available at GenBank. Available tissue samples from the positive cases were evaluated histopathologically. One out of 52 (1.9%, CI 95%, 0.0–5.7%) carnivores was positive; a roadkilled ocelot (Leopardus pardalis). The obtained sequence presented a low deduced amino acid (78.1%) similarity with the closest adenovirus, identified in a pinniped from the United States of America. This fact and its detection in a novel host suggest it may be representative of a novel species and denominated ocelot adenovirus 1. None of the gross and microscopic findings of the positive case were associated with adenovirus. To the authors' knowledge, this is the first report of adenovirus in wild felids of South America and the second worldwide. Further studies are necessary to assess the epidemiology and potential pathogenicity of this agent in wild carnivores.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Laboratory of Wildlife Comparative Pathology Department of Pathology School of Veterinary Medicine and Animal Sciences University of São Paulo, SPSchool of Veterinary Medicine and Animal Science Júlio de Mesquita Filho São Paulo State University - Botucatu campus, SPGroup of Epidemiology and Environmental Health Animal Health Research Centre (INIA-CISA), ValdeolmosVeterinary Department School of Biomedical and Health Sciences Universidad Europea de Madrid, C/Tajo s/n, Villaviciosa de OdónInstituto Adolfo Lutz, SPSchool of Veterinary Medicine and Animal Science Júlio de Mesquita Filho São Paulo State University - Botucatu campus, SPUniversidade de São Paulo (USP)Universidade Estadual Paulista (UNESP)Animal Health Research Centre (INIA-CISA)Universidad Europea de MadridInstituto Adolfo LutzLial, Henrique ChristinoNavas-Suárez, Pedro EnriqueEwbank, Ana CarolinaExposto Novoselecki, HelenaFerreira-Machado, Eduardo [UNESP]dos Santos Cirqueira, Cinthyade Azevedo Fernandes, Natália Coelho CoutoEsperón, FernandoCatão-Dias, José LuizSacristán, Carlos2022-04-28T19:50:49Z2022-04-28T19:50:49Z2022-04-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.meegid.2022.105246Infection, Genetics and Evolution, v. 99.1567-72571567-1348http://hdl.handle.net/11449/22346810.1016/j.meegid.2022.1052462-s2.0-85124605256Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengInfection, Genetics and Evolutioninfo:eu-repo/semantics/openAccess2022-04-28T19:50:49Zoai:repositorio.unesp.br:11449/223468Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462022-04-28T19:50:49Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Adenovirus surveillance in wild carnivores from Brazil |
title |
Adenovirus surveillance in wild carnivores from Brazil |
spellingShingle |
Adenovirus surveillance in wild carnivores from Brazil Lial, Henrique Christino Landscape transformation Ocelot Roadkill South America Viral discovery Wildlife |
title_short |
Adenovirus surveillance in wild carnivores from Brazil |
title_full |
Adenovirus surveillance in wild carnivores from Brazil |
title_fullStr |
Adenovirus surveillance in wild carnivores from Brazil |
title_full_unstemmed |
Adenovirus surveillance in wild carnivores from Brazil |
title_sort |
Adenovirus surveillance in wild carnivores from Brazil |
author |
Lial, Henrique Christino |
author_facet |
Lial, Henrique Christino Navas-Suárez, Pedro Enrique Ewbank, Ana Carolina Exposto Novoselecki, Helena Ferreira-Machado, Eduardo [UNESP] dos Santos Cirqueira, Cinthya de Azevedo Fernandes, Natália Coelho Couto Esperón, Fernando Catão-Dias, José Luiz Sacristán, Carlos |
author_role |
author |
author2 |
Navas-Suárez, Pedro Enrique Ewbank, Ana Carolina Exposto Novoselecki, Helena Ferreira-Machado, Eduardo [UNESP] dos Santos Cirqueira, Cinthya de Azevedo Fernandes, Natália Coelho Couto Esperón, Fernando Catão-Dias, José Luiz Sacristán, Carlos |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Universidade Estadual Paulista (UNESP) Animal Health Research Centre (INIA-CISA) Universidad Europea de Madrid Instituto Adolfo Lutz |
dc.contributor.author.fl_str_mv |
Lial, Henrique Christino Navas-Suárez, Pedro Enrique Ewbank, Ana Carolina Exposto Novoselecki, Helena Ferreira-Machado, Eduardo [UNESP] dos Santos Cirqueira, Cinthya de Azevedo Fernandes, Natália Coelho Couto Esperón, Fernando Catão-Dias, José Luiz Sacristán, Carlos |
dc.subject.por.fl_str_mv |
Landscape transformation Ocelot Roadkill South America Viral discovery Wildlife |
topic |
Landscape transformation Ocelot Roadkill South America Viral discovery Wildlife |
description |
Landscape transformation favors the spread of new pathogens that can be shared between domestic and wild animals. Certain adenoviruses (e.g., canine adenovirus 1 and 2, family Adenoviridae) can infect domestic and wild carnivores. In domestic canids, these viruses are associated with hepatic and respiratory diseases (among others). Nevertheless, information regarding adenovirus pathogenicity and molecular features in wild carnivores is still limited. Herein we surveyed adenovirus in free-ranging carnivores from Brazil. Total DNA was extracted from and subsequently tested by a nested panPCR in spleen and/or lung of 52 carnivores, representing species of the following families: Canidae (n = 4), Felidae (n = 3), Mustelidae (n = 2) and Procyonidae (n = 2). The obtained sequences were compared to others available at GenBank. Available tissue samples from the positive cases were evaluated histopathologically. One out of 52 (1.9%, CI 95%, 0.0–5.7%) carnivores was positive; a roadkilled ocelot (Leopardus pardalis). The obtained sequence presented a low deduced amino acid (78.1%) similarity with the closest adenovirus, identified in a pinniped from the United States of America. This fact and its detection in a novel host suggest it may be representative of a novel species and denominated ocelot adenovirus 1. None of the gross and microscopic findings of the positive case were associated with adenovirus. To the authors' knowledge, this is the first report of adenovirus in wild felids of South America and the second worldwide. Further studies are necessary to assess the epidemiology and potential pathogenicity of this agent in wild carnivores. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-04-28T19:50:49Z 2022-04-28T19:50:49Z 2022-04-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.meegid.2022.105246 Infection, Genetics and Evolution, v. 99. 1567-7257 1567-1348 http://hdl.handle.net/11449/223468 10.1016/j.meegid.2022.105246 2-s2.0-85124605256 |
url |
http://dx.doi.org/10.1016/j.meegid.2022.105246 http://hdl.handle.net/11449/223468 |
identifier_str_mv |
Infection, Genetics and Evolution, v. 99. 1567-7257 1567-1348 10.1016/j.meegid.2022.105246 2-s2.0-85124605256 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Infection, Genetics and Evolution |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1797790424992055296 |