Influência do clima nas características do sêmen e na composição proteica do plasma seminal em zebus e taurinos

• Autor(a) principal: Reis, Juliana Domingos Archanjo dos
• Data de Publicação: 2013
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Biblioteca Digital de Teses e Dissertações da UNOESTE
• Texto Completo: http://bdtd.unoeste.br:8080/tede/handle/tede/285
• topic: touros; estação do ano; sêmen; SDS-PAGE; fertilidade; bulls; season; semen; SDS-PAGE; fertility; CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA

Seminal plasma is a complex of secretions of the accessory reproductive organs of males with effects on sperm function. The quality of seminal plasma proteins may contribute to the fertility of bulls. The aim of this study was to determine the influence of season on the morphologic characteristics of the testes, epididymis, sperm and seminal plasma proteins by SDS-PAGE in Bos taurus indicus and Bos taurus taurus, bulls raised extensively. In this study 5 Nellore and 2 Simmental bulls, wewe used evaluated the scrotal and testicular morphometry, semen quality and the seminal plasma protein profile in SDS-PAGE in the dry and rainy seasons. Was obtained for a testicular volume TV = 460.14 cm3 during the rainy season and TV = 571.26 cm3 in the dry season. With respect to Zebu, in the rainy season the TV was 524.75 cm3 and 515.13 cm3 in the dry season. Sperm motility in the dry season for the taurine and total sperm defects in the rainy season for this same group of animals, showed as critical situation in the seminal analysis, suggesting the same attention when selecting bulls for purposes of natural mating. it is observed in 100% of the samples of the protein bands of 14 kDa, 21 kDa and 65 kDa in two stations evaluated. The seasons influence on morphology, and semen. The subspecies taurine showed great plasticity testicular volumes reflected in the testicle that reduces the rainy season. With respect to morphology, we found that there were significant effects of the dry season, improving the quality of sperm morphology. For electrophoresis, it was verified the presence of a large diversity of protein bands present in seminal plasma, and the bands 14, 21 and 65 kDa present in all the animals studied subspecies stations. It is suggested that the three protein bands found in all samples probably have beneficial action in relation to the framework sperm of bulls, collaborating with fertility.