Produção de lipases por microrganismos isolados de efluentes de laticínios através de fermentação submersa
Autor(a) principal: | |
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Data de Publicação: | 2007 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Biblioteca de teses e dissertações da Universidade de Passo Fundo (BDTD UPF) |
Texto Completo: | http://10.0.217.128:8080/jspui/handle/tede/249 |
Resumo: | The enzymatic production is one of the most promising fields inside of the technologies for the synthesis of compound from the high value added, being in constant growth for the great capacity of the microorganisms to carry out chemical transformations. Among the products gotten from fermentative processes through microorganisms are the enzymes. The enzymes produced from fermentative processes have been used for the ambient control for several ecosystems, being that many of these enzymes can be produced from industrial residues, reducing the costs production. The effluent of dairy is rich in slowly biodegradable fats that make difficult the treatment of these effluents in low temperatures. Lipases are a group of enzymes that catalyzes hydrolysis of long chains of triglycerides transforming them into glycerides and fatty acids. Microbial lipase has received great attention, constituting the most important group of enzymes for the biotechnological application. Lipases have being used in the reduction of the lipids concentration contained in the effluent, promoting hydrolysis of the presents oils and fats. The objective of this work was to evaluate the production of lipase from isolated fungus from the dairy effluent. The effluent was collected in the exit of the equalizer (P3) and the exit of the aerator (P6) from an effluent treatment station at a dairy industry in the region of Passo Fundo/RS being characterized as for DBO, DQO, phosphorous, nitrogen and oils and greases, in accordance with the methodology described at APHA (1995). It was carried out through the isolation of microorganisms from the collection points through techniques of series dilutions followed of the plating in medium PDA acidified and incubate at 30° C during 5 days. The isolated microorganisms had been identified through micro culture techniques. The selection of the fungi with potential of lipases production was evaluated from the growth of them in medium PDA added with olive oil as a carbon source |
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Hemkemeier, MarceloCPF:82074313934http://lattes.cnpq.br/1369101484238907Colla, Luciane MariaCPF:88888888888CPF:69550514072Roveda, Mirela2018-01-10T17:55:33Z2009-07-142007-07-06http://10.0.217.128:8080/jspui/handle/tede/249The enzymatic production is one of the most promising fields inside of the technologies for the synthesis of compound from the high value added, being in constant growth for the great capacity of the microorganisms to carry out chemical transformations. Among the products gotten from fermentative processes through microorganisms are the enzymes. The enzymes produced from fermentative processes have been used for the ambient control for several ecosystems, being that many of these enzymes can be produced from industrial residues, reducing the costs production. The effluent of dairy is rich in slowly biodegradable fats that make difficult the treatment of these effluents in low temperatures. Lipases are a group of enzymes that catalyzes hydrolysis of long chains of triglycerides transforming them into glycerides and fatty acids. Microbial lipase has received great attention, constituting the most important group of enzymes for the biotechnological application. Lipases have being used in the reduction of the lipids concentration contained in the effluent, promoting hydrolysis of the presents oils and fats. The objective of this work was to evaluate the production of lipase from isolated fungus from the dairy effluent. The effluent was collected in the exit of the equalizer (P3) and the exit of the aerator (P6) from an effluent treatment station at a dairy industry in the region of Passo Fundo/RS being characterized as for DBO, DQO, phosphorous, nitrogen and oils and greases, in accordance with the methodology described at APHA (1995). It was carried out through the isolation of microorganisms from the collection points through techniques of series dilutions followed of the plating in medium PDA acidified and incubate at 30° C during 5 days. The isolated microorganisms had been identified through micro culture techniques. The selection of the fungi with potential of lipases production was evaluated from the growth of them in medium PDA added with olive oil as a carbon sourceA produção enzimática é um dos campos mais promissores dentro das tecnologias para a síntese de compostos de alto valor agregado, estando em constante crescimento pela grande capacidade dos microrganismos de realizarem transformações químicas. Dentre os produtos obtidos via processos fermentativos através de microrganismos estão as enzimas. As enzimas produzidas por processos fermentativos tem sido utilizadas para o controle ambiental de vários ecossistemas, sendo que muitas destas enzimas podem ser produzidas a partir de resíduos industriais, diminuindo os custos de produção. Os efluentes de laticínios são ricos em gorduras lentamente biodegradáveis, o que dificulta o tratamento destes efluentes em baixas temperaturas. As lipases são enzimas que catalisam a hidrólise de longas cadeias de triglicerídeos transformando-os em glicerídeos e ácidos graxos. As lipases microbianas têm recebido grande atenção, constituindo o mais importante grupo de enzimas para a aplicação biotecnológica. As lipases vêm sendo utilizadas na redução da concentração dos lipídios contidos nos efluentes, promovendo a hidrólise dos óleos e gorduras presentes. Objetivou-se avaliar a produção de lipases por fungos isolados a partir de efluentes de laticínios. O efluente foi coletado na saída do equalizador (P3) e saída do aerador (P6) de uma estação de tratamento de efluentes de laticínios da região de Passo Fundo, sendo caracterizado quanto a DBO, DQO, fósforo, nitrogênio e óleos e graxas, de acordo com a metodologia descrita por APHA (1995). Foi realizado o isolamento de microrganismos dos pontos de coleta através de técnicas de diluições seriadas seguidas de plaqueamento em meio PDA acidificado e incubação a 30° C durante 5 dias. Os microrganismos isolados foram identificados através de técnicas de microcultivo. A seleção dos fungos com potencial de produção de lipases foi avaliada a partir do crescimento dos mesmos em meio PDA contento azeite de oliva como fonte de carbonoMade available in DSpace on 2018-01-10T17:55:33Z (GMT). No. of bitstreams: 1 2007MirelaRoveda.pdf: 808707 bytes, checksum: 047286243b8e30873b1e1d5abca2d372 (MD5) Previous issue date: 2007-07-06application/pdfporPrograma de Pós-Graduação em EngenhariaEngenhariasResíduos industriais - Aspectos ambientaisEnzimasLipídiosGorduraFactory and trade wasteFatLipidsEnzymesCNPQ::ENGENHARIAS::ENGENHARIA MECANICA::ENGENHARIA TERMICA::CONTROLE AMBIENTALProdução de lipases por microrganismos isolados de efluentes de laticínios através de fermentação submersainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:Biblioteca de teses e dissertações da Universidade de Passo Fundo (BDTD UPF)instname:Universidade de Passo Fundo (UPF)instacron:UPFORIGINAL2007MirelaRoveda.pdfapplication/pdf808707http://tede.upf.br:8080/jspui/bitstream/tede/249/1/2007MirelaRoveda.pdf047286243b8e30873b1e1d5abca2d372MD51tede/2492018-01-10 15:55:33.938oai:tede.upf.br:tede/249Biblioteca Digital de Teses e DissertaçõesPUBhttp://tede.upf.br/oai/requestbiblio@upf.br || bio@upf.br || cas@upf.br || car@upf.br || lve@upf.br || sar@upf.br || sol@upf.br || upfmundi@upf.br || jucelei@upf.bropendoar:2018-01-10T17:55:33Biblioteca de teses e dissertações da Universidade de Passo Fundo (BDTD UPF) - Universidade de Passo Fundo (UPF)false |
dc.title.por.fl_str_mv |
Produção de lipases por microrganismos isolados de efluentes de laticínios através de fermentação submersa |
title |
Produção de lipases por microrganismos isolados de efluentes de laticínios através de fermentação submersa |
spellingShingle |
Produção de lipases por microrganismos isolados de efluentes de laticínios através de fermentação submersa Roveda, Mirela Resíduos industriais - Aspectos ambientais Enzimas Lipídios Gordura Factory and trade waste Fat Lipids Enzymes CNPQ::ENGENHARIAS::ENGENHARIA MECANICA::ENGENHARIA TERMICA::CONTROLE AMBIENTAL |
title_short |
Produção de lipases por microrganismos isolados de efluentes de laticínios através de fermentação submersa |
title_full |
Produção de lipases por microrganismos isolados de efluentes de laticínios através de fermentação submersa |
title_fullStr |
Produção de lipases por microrganismos isolados de efluentes de laticínios através de fermentação submersa |
title_full_unstemmed |
Produção de lipases por microrganismos isolados de efluentes de laticínios através de fermentação submersa |
title_sort |
Produção de lipases por microrganismos isolados de efluentes de laticínios através de fermentação submersa |
author |
Roveda, Mirela |
author_facet |
Roveda, Mirela |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Hemkemeier, Marcelo |
dc.contributor.advisor1ID.fl_str_mv |
CPF:82074313934 |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/1369101484238907 |
dc.contributor.advisor-co1.fl_str_mv |
Colla, Luciane Maria |
dc.contributor.advisor-co1ID.fl_str_mv |
CPF:88888888888 |
dc.contributor.authorID.fl_str_mv |
CPF:69550514072 |
dc.contributor.author.fl_str_mv |
Roveda, Mirela |
contributor_str_mv |
Hemkemeier, Marcelo Colla, Luciane Maria |
dc.subject.por.fl_str_mv |
Resíduos industriais - Aspectos ambientais Enzimas Lipídios Gordura |
topic |
Resíduos industriais - Aspectos ambientais Enzimas Lipídios Gordura Factory and trade waste Fat Lipids Enzymes CNPQ::ENGENHARIAS::ENGENHARIA MECANICA::ENGENHARIA TERMICA::CONTROLE AMBIENTAL |
dc.subject.eng.fl_str_mv |
Factory and trade waste Fat Lipids Enzymes |
dc.subject.cnpq.fl_str_mv |
CNPQ::ENGENHARIAS::ENGENHARIA MECANICA::ENGENHARIA TERMICA::CONTROLE AMBIENTAL |
description |
The enzymatic production is one of the most promising fields inside of the technologies for the synthesis of compound from the high value added, being in constant growth for the great capacity of the microorganisms to carry out chemical transformations. Among the products gotten from fermentative processes through microorganisms are the enzymes. The enzymes produced from fermentative processes have been used for the ambient control for several ecosystems, being that many of these enzymes can be produced from industrial residues, reducing the costs production. The effluent of dairy is rich in slowly biodegradable fats that make difficult the treatment of these effluents in low temperatures. Lipases are a group of enzymes that catalyzes hydrolysis of long chains of triglycerides transforming them into glycerides and fatty acids. Microbial lipase has received great attention, constituting the most important group of enzymes for the biotechnological application. Lipases have being used in the reduction of the lipids concentration contained in the effluent, promoting hydrolysis of the presents oils and fats. The objective of this work was to evaluate the production of lipase from isolated fungus from the dairy effluent. The effluent was collected in the exit of the equalizer (P3) and the exit of the aerator (P6) from an effluent treatment station at a dairy industry in the region of Passo Fundo/RS being characterized as for DBO, DQO, phosphorous, nitrogen and oils and greases, in accordance with the methodology described at APHA (1995). It was carried out through the isolation of microorganisms from the collection points through techniques of series dilutions followed of the plating in medium PDA acidified and incubate at 30° C during 5 days. The isolated microorganisms had been identified through micro culture techniques. The selection of the fungi with potential of lipases production was evaluated from the growth of them in medium PDA added with olive oil as a carbon source |
publishDate |
2007 |
dc.date.issued.fl_str_mv |
2007-07-06 |
dc.date.available.fl_str_mv |
2009-07-14 |
dc.date.accessioned.fl_str_mv |
2018-01-10T17:55:33Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/masterThesis |
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masterThesis |
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publishedVersion |
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http://10.0.217.128:8080/jspui/handle/tede/249 |
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http://10.0.217.128:8080/jspui/handle/tede/249 |
dc.language.iso.fl_str_mv |
por |
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por |
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info:eu-repo/semantics/openAccess |
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openAccess |
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dc.publisher.por.fl_str_mv |
|
dc.publisher.program.fl_str_mv |
Programa de Pós-Graduação em Engenharia |
dc.publisher.department.fl_str_mv |
Engenharias |
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|
dc.publisher.initials.por.fl_str_mv |
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dc.source.none.fl_str_mv |
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Biblioteca de teses e dissertações da Universidade de Passo Fundo (BDTD UPF) |
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biblio@upf.br || bio@upf.br || cas@upf.br || car@upf.br || lve@upf.br || sar@upf.br || sol@upf.br || upfmundi@upf.br || jucelei@upf.br |
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