Efeito do estanozolol sobre a fertilidade em ratas pré-púberes
Main Author: | |
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Publication Date: | 2013 |
Format: | Master thesis |
Language: | por |
Source: | Biblioteca Digital de Teses e Dissertações da UFRPE |
Download full: | http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/4639 |
Summary: | The indiscriminate use of anabolic androgenic steroids (AAS) has becoming part of young and practitioners of physical activities’ daily life, especially at gyms and other places where people practice some exercise. First, It had only male users. It also has reached a female audience. Some unwanted collateral effects in adult women are result from the AAS using, however, the physiological effects caused by the indiscriminate use of AAS by female adolescents in the puberty beginning are still unknown, and there’s no study about the permanency of possible effects on the reproductive life. Thus, this study analyzed the stanozolol effect in prepubertal female rats on fertility using the following parameters: estrous cyclicity, vaginal opening, sex hormones and their receptors, implantation sites number, ovarian and placental histology, besides the weight and angiogenesis and these organs. 20 female albino rats were used, and they were submitted to the following treatments: Control group - prepubertal female rats sesame oil treated and Stanozolol group - prepubertal female rats stanozolol treated, the stanozolol was used in an only subcutaneous injection 5mg/Kg with sesame oil 1mL/kg as vehicle, in the twenty-first day after birth. Results showed that even used in low quantities and only once stanozolol promoted an earlier vaginal opening, significant increase in the proestrus and estrus frequency and decline in the metestrous and diestrus, an estrogenic levels increase and reduction of progestagen levels, expression decrease from estrogen alpha receptor in the ovaries, reduction of implantation sites, ovarian and placental weight increase, besides the ovarian and placental VEGF higher expression. Therefore, the study concluded that the prepubertal stanozolol using acts as an endocrine disruptor on rats reproduction. |
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Teixeira, Álvaro Aguiar CoelhoTeixeira, Valéria WanderleyAlves, Luiz CarlosSoares, Anísio Franciscohttp://lattes.cnpq.br/1578785711485119ELIAS, Laís de Souza2016-06-09T11:30:06Z2013-02-08ELIAS, Laís de Souza. Efeito do estanozolol sobre a fertilidade em ratas pré-púberes. 2013. 62 f. Dissertação (Programa de Pós-Graduação em Biociência Animal) - Universidade Federal Rural de Pernambuco, Recife.http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/4639The indiscriminate use of anabolic androgenic steroids (AAS) has becoming part of young and practitioners of physical activities’ daily life, especially at gyms and other places where people practice some exercise. First, It had only male users. It also has reached a female audience. Some unwanted collateral effects in adult women are result from the AAS using, however, the physiological effects caused by the indiscriminate use of AAS by female adolescents in the puberty beginning are still unknown, and there’s no study about the permanency of possible effects on the reproductive life. Thus, this study analyzed the stanozolol effect in prepubertal female rats on fertility using the following parameters: estrous cyclicity, vaginal opening, sex hormones and their receptors, implantation sites number, ovarian and placental histology, besides the weight and angiogenesis and these organs. 20 female albino rats were used, and they were submitted to the following treatments: Control group - prepubertal female rats sesame oil treated and Stanozolol group - prepubertal female rats stanozolol treated, the stanozolol was used in an only subcutaneous injection 5mg/Kg with sesame oil 1mL/kg as vehicle, in the twenty-first day after birth. Results showed that even used in low quantities and only once stanozolol promoted an earlier vaginal opening, significant increase in the proestrus and estrus frequency and decline in the metestrous and diestrus, an estrogenic levels increase and reduction of progestagen levels, expression decrease from estrogen alpha receptor in the ovaries, reduction of implantation sites, ovarian and placental weight increase, besides the ovarian and placental VEGF higher expression. Therefore, the study concluded that the prepubertal stanozolol using acts as an endocrine disruptor on rats reproduction.O uso indiscriminado de esteróides anabolizantes androgênicos (EAA) vem fazendo parte da rotina de jovens e praticantes de atividade física, principalmente em academias ou centro de práticas esportivas. O que antes era restrito apenas para o público masculino, tem alcançado também o público feminino. Esse uso tem resultado em uma série de efeitos colaterais indesejáveis em mulheres adultas, no entanto, os efeitos fisiológicos do abuso destes por adolescentes do sexo feminino no início da puberdade são ainda desconhecidos, e não há estudo de que os possíveis efeitos possam ser permanentes afetando sua vida reprodutiva. Assim, a presente pesquisa analisou o efeito do estanozolol administrado em ratas prépúberes sobre a fertilidade através dos seguintes parâmetros: ciclicidade estral, abertura vaginal, hormônios sexuais, receptores de estrógenos, número de sítios de implantação, histologia ovariana e placentária, além do peso e angiogênese nesses órgãos. Foram utilizadas 20 ratas albinas, as quais foram submetidas aos seguintes tratamentos: ratas pré-púberes tratadas com óleo de gergelim (GI) e ratas prépúberes tratadas com estanozolol (GII), administrado em uma única injeção s.c. de 5mg/Kg em óleo de gergelim (1mL/kg) como veículo, no vigésimo primeiro dia após nascimento. Os resultados mostraram que o estanozolol mesmo administrado em dose muito baixa e numa única vez promoveu abertura vaginal precocemente, aumento significativo na frequência das fases de proestro e estro e declínio das fases de metaestro e diestro, elevação dos níveis estrogênicos e redução dos níveis progestagênicos, redução da expressão do receptor-α estrogênico nos ovários, redução do número de sítios de implantação, aumento do peso ovariano e placentário, além da maior expressão do VEGF placentário e ovariano. Assim, conclui-se que o estanozolol administrado antes da puberdade atua como um desregulador endócrino da reprodução em ratas.Submitted by (lucia.rodrigues@ufrpe.br) on 2016-06-09T11:30:06Z No. of bitstreams: 1 Laise de Souza Elias.pdf: 816284 bytes, checksum: 5cfeb66cf7be781496cd7de48b41b8e1 (MD5)Made available in DSpace on 2016-06-09T11:30:06Z (GMT). No. of bitstreams: 1 Laise de Souza Elias.pdf: 816284 bytes, checksum: 5cfeb66cf7be781496cd7de48b41b8e1 (MD5) Previous issue date: 2013-02-08application/pdfporUniversidade Federal Rural de PernambucoPrograma de Pós-Graduação em Biociência AnimalUFRPEBrasilDepartamento de Morfologia e Fisiologia AnimalAnabolizanteReprodução animalReceptoresAngiogêneseRoedoresAnabolicsAnimal reproductionReceptorsAngiogenesisPrepubertal femaleRatsCIENCIAS AGRARIAS::MEDICINA VETERINARIAEfeito do estanozolol sobre a fertilidade em ratas pré-púberesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis-1510757014399315592600600600-8922364187987396204453670264235017319info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UFRPEinstname:Universidade Federal Rural de Pernambuco (UFRPE)instacron:UFRPELICENSElicense.txtlicense.txttext/plain; charset=utf-82165http://www.tede2.ufrpe.br:8080/tede2/bitstream/tede2/4639/1/license.txtbd3efa91386c1718a7f26a329fdcb468MD51ORIGINALLaise de Souza Elias.pdfLaise de Souza Elias.pdfapplication/pdf816284http://www.tede2.ufrpe.br:8080/tede2/bitstream/tede2/4639/2/Laise+de+Souza+Elias.pdf5cfeb66cf7be781496cd7de48b41b8e1MD52tede2/46392018-04-09 10:55:12.672oai:tede2:tede2/4639Tk9UQTogQ09MT1FVRSBBUVVJIEEgU1VBIFBSw5NQUklBIExJQ0VOw4dBCkVzdGEgbGljZW7Dp2EgZGUgZXhlbXBsbyDDqSBmb3JuZWNpZGEgYXBlbmFzIHBhcmEgZmlucyBpbmZvcm1hdGl2b3MuCgpMSUNFTsOHQSBERSBESVNUUklCVUnDh8ODTyBOw4NPLUVYQ0xVU0lWQQoKQ29tIGEgYXByZXNlbnRhw6fDo28gZGVzdGEgbGljZW7Dp2EsIHZvY8OqIChvIGF1dG9yIChlcykgb3UgbyB0aXR1bGFyIGRvcyBkaXJlaXRvcyBkZSBhdXRvcikgY29uY2VkZSDDoCBVbml2ZXJzaWRhZGUgClhYWCAoU2lnbGEgZGEgVW5pdmVyc2lkYWRlKSBvIGRpcmVpdG8gbsOjby1leGNsdXNpdm8gZGUgcmVwcm9kdXppciwgIHRyYWR1emlyIChjb25mb3JtZSBkZWZpbmlkbyBhYmFpeG8pLCBlL291IApkaXN0cmlidWlyIGEgc3VhIHRlc2Ugb3UgZGlzc2VydGHDp8OjbyAoaW5jbHVpbmRvIG8gcmVzdW1vKSBwb3IgdG9kbyBvIG11bmRvIG5vIGZvcm1hdG8gaW1wcmVzc28gZSBlbGV0csO0bmljbyBlIAplbSBxdWFscXVlciBtZWlvLCBpbmNsdWluZG8gb3MgZm9ybWF0b3Mgw6F1ZGlvIG91IHbDrWRlby4KClZvY8OqIGNvbmNvcmRhIHF1ZSBhIFNpZ2xhIGRlIFVuaXZlcnNpZGFkZSBwb2RlLCBzZW0gYWx0ZXJhciBvIGNvbnRlw7pkbywgdHJhbnNwb3IgYSBzdWEgdGVzZSBvdSBkaXNzZXJ0YcOnw6NvIApwYXJhIHF1YWxxdWVyIG1laW8gb3UgZm9ybWF0byBwYXJhIGZpbnMgZGUgcHJlc2VydmHDp8Ojby4KClZvY8OqIHRhbWLDqW0gY29uY29yZGEgcXVlIGEgU2lnbGEgZGUgVW5pdmVyc2lkYWRlIHBvZGUgbWFudGVyIG1haXMgZGUgdW1hIGPDs3BpYSBhIHN1YSB0ZXNlIG91IApkaXNzZXJ0YcOnw6NvIHBhcmEgZmlucyBkZSBzZWd1cmFuw6dhLCBiYWNrLXVwIGUgcHJlc2VydmHDp8Ojby4KClZvY8OqIGRlY2xhcmEgcXVlIGEgc3VhIHRlc2Ugb3UgZGlzc2VydGHDp8OjbyDDqSBvcmlnaW5hbCBlIHF1ZSB2b2PDqiB0ZW0gbyBwb2RlciBkZSBjb25jZWRlciBvcyBkaXJlaXRvcyBjb250aWRvcyAKbmVzdGEgbGljZW7Dp2EuIFZvY8OqIHRhbWLDqW0gZGVjbGFyYSBxdWUgbyBkZXDDs3NpdG8gZGEgc3VhIHRlc2Ugb3UgZGlzc2VydGHDp8OjbyBuw6NvLCBxdWUgc2VqYSBkZSBzZXUgCmNvbmhlY2ltZW50bywgaW5mcmluZ2UgZGlyZWl0b3MgYXV0b3JhaXMgZGUgbmluZ3XDqW0uCgpDYXNvIGEgc3VhIHRlc2Ugb3UgZGlzc2VydGHDp8OjbyBjb250ZW5oYSBtYXRlcmlhbCBxdWUgdm9jw6ogbsOjbyBwb3NzdWkgYSB0aXR1bGFyaWRhZGUgZG9zIGRpcmVpdG9zIGF1dG9yYWlzLCB2b2PDqiAKZGVjbGFyYSBxdWUgb2J0ZXZlIGEgcGVybWlzc8OjbyBpcnJlc3RyaXRhIGRvIGRldGVudG9yIGRvcyBkaXJlaXRvcyBhdXRvcmFpcyBwYXJhIGNvbmNlZGVyIMOgIFNpZ2xhIGRlIFVuaXZlcnNpZGFkZSAKb3MgZGlyZWl0b3MgYXByZXNlbnRhZG9zIG5lc3RhIGxpY2Vuw6dhLCBlIHF1ZSBlc3NlIG1hdGVyaWFsIGRlIHByb3ByaWVkYWRlIGRlIHRlcmNlaXJvcyBlc3TDoSBjbGFyYW1lbnRlIAppZGVudGlmaWNhZG8gZSByZWNvbmhlY2lkbyBubyB0ZXh0byBvdSBubyBjb250ZcO6ZG8gZGEgdGVzZSBvdSBkaXNzZXJ0YcOnw6NvIG9yYSBkZXBvc2l0YWRhLgoKQ0FTTyBBIFRFU0UgT1UgRElTU0VSVEHDh8ODTyBPUkEgREVQT1NJVEFEQSBURU5IQSBTSURPIFJFU1VMVEFETyBERSBVTSBQQVRST0PDjU5JTyBPVSAKQVBPSU8gREUgVU1BIEFHw4pOQ0lBIERFIEZPTUVOVE8gT1UgT1VUUk8gT1JHQU5JU01PIFFVRSBOw4NPIFNFSkEgQSBTSUdMQSBERSAKVU5JVkVSU0lEQURFLCBWT0PDiiBERUNMQVJBIFFVRSBSRVNQRUlUT1UgVE9ET1MgRSBRVUFJU1FVRVIgRElSRUlUT1MgREUgUkVWSVPDg08gQ09NTyAKVEFNQsOJTSBBUyBERU1BSVMgT0JSSUdBw4fDlUVTIEVYSUdJREFTIFBPUiBDT05UUkFUTyBPVSBBQ09SRE8uCgpBIFNpZ2xhIGRlIFVuaXZlcnNpZGFkZSBzZSBjb21wcm9tZXRlIGEgaWRlbnRpZmljYXIgY2xhcmFtZW50ZSBvIHNldSBub21lIChzKSBvdSBvKHMpIG5vbWUocykgZG8ocykgCmRldGVudG9yKGVzKSBkb3MgZGlyZWl0b3MgYXV0b3JhaXMgZGEgdGVzZSBvdSBkaXNzZXJ0YcOnw6NvLCBlIG7Do28gZmFyw6EgcXVhbHF1ZXIgYWx0ZXJhw6fDo28sIGFsw6ltIGRhcXVlbGFzIApjb25jZWRpZGFzIHBvciBlc3RhIGxpY2Vuw6dhLgo=Biblioteca Digital de Teses e Dissertaçõeshttp://www.tede2.ufrpe.br:8080/tede/PUBhttp://www.tede2.ufrpe.br:8080/oai/requestbdtd@ufrpe.br ||bdtd@ufrpe.bropendoar:2018-04-09T13:55:12Biblioteca Digital de Teses e Dissertações da UFRPE - Universidade Federal Rural de Pernambuco (UFRPE)false |
dc.title.por.fl_str_mv |
Efeito do estanozolol sobre a fertilidade em ratas pré-púberes |
title |
Efeito do estanozolol sobre a fertilidade em ratas pré-púberes |
spellingShingle |
Efeito do estanozolol sobre a fertilidade em ratas pré-púberes ELIAS, Laís de Souza Anabolizante Reprodução animal Receptores Angiogênese Roedores Anabolics Animal reproduction Receptors Angiogenesis Prepubertal female Rats CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
title_short |
Efeito do estanozolol sobre a fertilidade em ratas pré-púberes |
title_full |
Efeito do estanozolol sobre a fertilidade em ratas pré-púberes |
title_fullStr |
Efeito do estanozolol sobre a fertilidade em ratas pré-púberes |
title_full_unstemmed |
Efeito do estanozolol sobre a fertilidade em ratas pré-púberes |
title_sort |
Efeito do estanozolol sobre a fertilidade em ratas pré-púberes |
author |
ELIAS, Laís de Souza |
author_facet |
ELIAS, Laís de Souza |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Teixeira, Álvaro Aguiar Coelho |
dc.contributor.advisor-co1.fl_str_mv |
Teixeira, Valéria Wanderley |
dc.contributor.referee1.fl_str_mv |
Alves, Luiz Carlos |
dc.contributor.referee2.fl_str_mv |
Soares, Anísio Francisco |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/1578785711485119 |
dc.contributor.author.fl_str_mv |
ELIAS, Laís de Souza |
contributor_str_mv |
Teixeira, Álvaro Aguiar Coelho Teixeira, Valéria Wanderley Alves, Luiz Carlos Soares, Anísio Francisco |
dc.subject.por.fl_str_mv |
Anabolizante Reprodução animal Receptores Angiogênese Roedores |
topic |
Anabolizante Reprodução animal Receptores Angiogênese Roedores Anabolics Animal reproduction Receptors Angiogenesis Prepubertal female Rats CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
dc.subject.eng.fl_str_mv |
Anabolics Animal reproduction Receptors Angiogenesis Prepubertal female Rats |
dc.subject.cnpq.fl_str_mv |
CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
description |
The indiscriminate use of anabolic androgenic steroids (AAS) has becoming part of young and practitioners of physical activities’ daily life, especially at gyms and other places where people practice some exercise. First, It had only male users. It also has reached a female audience. Some unwanted collateral effects in adult women are result from the AAS using, however, the physiological effects caused by the indiscriminate use of AAS by female adolescents in the puberty beginning are still unknown, and there’s no study about the permanency of possible effects on the reproductive life. Thus, this study analyzed the stanozolol effect in prepubertal female rats on fertility using the following parameters: estrous cyclicity, vaginal opening, sex hormones and their receptors, implantation sites number, ovarian and placental histology, besides the weight and angiogenesis and these organs. 20 female albino rats were used, and they were submitted to the following treatments: Control group - prepubertal female rats sesame oil treated and Stanozolol group - prepubertal female rats stanozolol treated, the stanozolol was used in an only subcutaneous injection 5mg/Kg with sesame oil 1mL/kg as vehicle, in the twenty-first day after birth. Results showed that even used in low quantities and only once stanozolol promoted an earlier vaginal opening, significant increase in the proestrus and estrus frequency and decline in the metestrous and diestrus, an estrogenic levels increase and reduction of progestagen levels, expression decrease from estrogen alpha receptor in the ovaries, reduction of implantation sites, ovarian and placental weight increase, besides the ovarian and placental VEGF higher expression. Therefore, the study concluded that the prepubertal stanozolol using acts as an endocrine disruptor on rats reproduction. |
publishDate |
2013 |
dc.date.issued.fl_str_mv |
2013-02-08 |
dc.date.accessioned.fl_str_mv |
2016-06-09T11:30:06Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
ELIAS, Laís de Souza. Efeito do estanozolol sobre a fertilidade em ratas pré-púberes. 2013. 62 f. Dissertação (Programa de Pós-Graduação em Biociência Animal) - Universidade Federal Rural de Pernambuco, Recife. |
dc.identifier.uri.fl_str_mv |
http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/4639 |
identifier_str_mv |
ELIAS, Laís de Souza. Efeito do estanozolol sobre a fertilidade em ratas pré-púberes. 2013. 62 f. Dissertação (Programa de Pós-Graduação em Biociência Animal) - Universidade Federal Rural de Pernambuco, Recife. |
url |
http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/4639 |
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por |
language |
por |
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600 600 600 |
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dc.relation.cnpq.fl_str_mv |
453670264235017319 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal Rural de Pernambuco |
dc.publisher.program.fl_str_mv |
Programa de Pós-Graduação em Biociência Animal |
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UFRPE |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Departamento de Morfologia e Fisiologia Animal |
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Universidade Federal Rural de Pernambuco |
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