Effect of topical ozonetherapy on gingival wound healing in pigs: histological and immuno-histochemical analysis
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Journal of applied oral science (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572019000100407 |
Resumo: | Abstract In this study, the effects of ozonetherapy on secondary wound healing were evaluated histologically and immuno-histochemically. Material and Methods: 8 healthy pigs were used in this study. Six wounds with 10 mm in diameter were created through the punch technique on the palatinal gingiva of each pig. Ozone gas was applied on only 3 wounds (test group) and the remaining 3 were left to natural healing (control group). Biopsy samples were taken from one of the wounds in each group on the third day, from another wound of each group on the seventh day, and from another one on the tenth day. Routine histological analysis and immuno-histochemical staining were performed to investigate transforming growth factor-beta (TGF-β) and (VEGF) expressions. Results: No statistical difference was found between the test and control groups in terms of collagen fibers, epithelial formation and inflammation scores. A VEGF expression found in the test group was statistically higher than control group samples taken on the 3rd and 7th day. There was no statistical difference between the test and control groups in terms of TGF-β expression on any of the sampling days. Conclusion: The topical application of ozone gas could be effective in the early stages of wound healing by increasing the amount of VEGF expression. Clinical Relevance: Topical application of ozone gas may be effective in the early stages of oral wound healing. |
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Journal of applied oral science (Online) |
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Effect of topical ozonetherapy on gingival wound healing in pigs: histological and immuno-histochemical analysisOzoneTransforming growth factor beta (TNF-β)Vascular endothelial growth factor (VEGF)Abstract In this study, the effects of ozonetherapy on secondary wound healing were evaluated histologically and immuno-histochemically. Material and Methods: 8 healthy pigs were used in this study. Six wounds with 10 mm in diameter were created through the punch technique on the palatinal gingiva of each pig. Ozone gas was applied on only 3 wounds (test group) and the remaining 3 were left to natural healing (control group). Biopsy samples were taken from one of the wounds in each group on the third day, from another wound of each group on the seventh day, and from another one on the tenth day. Routine histological analysis and immuno-histochemical staining were performed to investigate transforming growth factor-beta (TGF-β) and (VEGF) expressions. Results: No statistical difference was found between the test and control groups in terms of collagen fibers, epithelial formation and inflammation scores. A VEGF expression found in the test group was statistically higher than control group samples taken on the 3rd and 7th day. There was no statistical difference between the test and control groups in terms of TGF-β expression on any of the sampling days. Conclusion: The topical application of ozone gas could be effective in the early stages of wound healing by increasing the amount of VEGF expression. Clinical Relevance: Topical application of ozone gas may be effective in the early stages of oral wound healing.Faculdade De Odontologia De Bauru - USP2019-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572019000100407Journal of Applied Oral Science v.27 2019reponame:Journal of applied oral science (Online)instname:Universidade de São Paulo (USP)instacron:USP10.1590/1678-7757-2018-0015info:eu-repo/semantics/openAccessEroglu,Zeynep TastanKurtis,BulentAltug,Hasan AyberkSahin,SermetTuter,GulayBaris,Emreeng2018-12-06T00:00:00Zoai:scielo:S1678-77572019000100407Revistahttp://www.scielo.br/jaosPUBhttps://old.scielo.br/oai/scielo-oai.php||jaos@usp.br1678-77651678-7757opendoar:2018-12-06T00:00Journal of applied oral science (Online) - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Effect of topical ozonetherapy on gingival wound healing in pigs: histological and immuno-histochemical analysis |
title |
Effect of topical ozonetherapy on gingival wound healing in pigs: histological and immuno-histochemical analysis |
spellingShingle |
Effect of topical ozonetherapy on gingival wound healing in pigs: histological and immuno-histochemical analysis Eroglu,Zeynep Tastan Ozone Transforming growth factor beta (TNF-β) Vascular endothelial growth factor (VEGF) |
title_short |
Effect of topical ozonetherapy on gingival wound healing in pigs: histological and immuno-histochemical analysis |
title_full |
Effect of topical ozonetherapy on gingival wound healing in pigs: histological and immuno-histochemical analysis |
title_fullStr |
Effect of topical ozonetherapy on gingival wound healing in pigs: histological and immuno-histochemical analysis |
title_full_unstemmed |
Effect of topical ozonetherapy on gingival wound healing in pigs: histological and immuno-histochemical analysis |
title_sort |
Effect of topical ozonetherapy on gingival wound healing in pigs: histological and immuno-histochemical analysis |
author |
Eroglu,Zeynep Tastan |
author_facet |
Eroglu,Zeynep Tastan Kurtis,Bulent Altug,Hasan Ayberk Sahin,Sermet Tuter,Gulay Baris,Emre |
author_role |
author |
author2 |
Kurtis,Bulent Altug,Hasan Ayberk Sahin,Sermet Tuter,Gulay Baris,Emre |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Eroglu,Zeynep Tastan Kurtis,Bulent Altug,Hasan Ayberk Sahin,Sermet Tuter,Gulay Baris,Emre |
dc.subject.por.fl_str_mv |
Ozone Transforming growth factor beta (TNF-β) Vascular endothelial growth factor (VEGF) |
topic |
Ozone Transforming growth factor beta (TNF-β) Vascular endothelial growth factor (VEGF) |
description |
Abstract In this study, the effects of ozonetherapy on secondary wound healing were evaluated histologically and immuno-histochemically. Material and Methods: 8 healthy pigs were used in this study. Six wounds with 10 mm in diameter were created through the punch technique on the palatinal gingiva of each pig. Ozone gas was applied on only 3 wounds (test group) and the remaining 3 were left to natural healing (control group). Biopsy samples were taken from one of the wounds in each group on the third day, from another wound of each group on the seventh day, and from another one on the tenth day. Routine histological analysis and immuno-histochemical staining were performed to investigate transforming growth factor-beta (TGF-β) and (VEGF) expressions. Results: No statistical difference was found between the test and control groups in terms of collagen fibers, epithelial formation and inflammation scores. A VEGF expression found in the test group was statistically higher than control group samples taken on the 3rd and 7th day. There was no statistical difference between the test and control groups in terms of TGF-β expression on any of the sampling days. Conclusion: The topical application of ozone gas could be effective in the early stages of wound healing by increasing the amount of VEGF expression. Clinical Relevance: Topical application of ozone gas may be effective in the early stages of oral wound healing. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572019000100407 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572019000100407 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/1678-7757-2018-0015 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Faculdade De Odontologia De Bauru - USP |
publisher.none.fl_str_mv |
Faculdade De Odontologia De Bauru - USP |
dc.source.none.fl_str_mv |
Journal of Applied Oral Science v.27 2019 reponame:Journal of applied oral science (Online) instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Journal of applied oral science (Online) |
collection |
Journal of applied oral science (Online) |
repository.name.fl_str_mv |
Journal of applied oral science (Online) - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
||jaos@usp.br |
_version_ |
1748936440000020480 |