Avaliação do efeito radioprotetor da carnosina (beta-alanil-1- histidina) no processo de cicatrização em ratos
Main Author: | |
---|---|
Publication Date: | 2005 |
Other Authors: | , , , |
Format: | Article |
Language: | eng |
Source: | Journal of applied oral science (Online) |
Download full: | https://www.revistas.usp.br/jaos/article/view/3288 |
Summary: | The purpose of this study was to evaluate the radioprotective effect of carnosine (beta- alanyl-1-histidine) on the wound healing in rats. Therefore, 48 male rats were submitted to a surgical procedure to perform a rectangular wound in the anterior-dorsal region. The animals were divided into 4 experimental groups randomly chosen: control; irradiated; carnosine irradiated and carnosine group. The irradiated and carnosine irradiated group were exposed to a dose (6Gy) of gamma irradiation, in the whole body, 72 hours after surgery. The carnosine and carnosine irradiated groups, in addition to the surgical procedure and the irradiation, received two doses of carnosine aqueous solution, the first one being injected 48 hours after surgery, and the second one 1 hour and 30 minutes before irradiation. The tissue repair of the 4 groups was evaluated at 4, 7, 14, and 21 days after inflicting the wound, by morphological, histochemical and histophysical methods. At all examined periods, it could be observed that the animals from the carnosine irradiated group presented a better developed granulation tissue than the irradiated group and closely similar to that of the control group. Thus, under the experimental conditions used, it was possible to conclude that carnosine is an effective radioprotective substance. |
id |
USP-17_1071c59a95ee0a71c279ca92e2dcb617 |
---|---|
oai_identifier_str |
oai:revistas.usp.br:article/3288 |
network_acronym_str |
USP-17 |
network_name_str |
Journal of applied oral science (Online) |
repository_id_str |
|
spelling |
Avaliação do efeito radioprotetor da carnosina (beta-alanil-1- histidina) no processo de cicatrização em ratos Evaluation of radioprotective effect of carnosine (beta- alanyl-1- histidine) on the wound healing in rats RadiationionizingRadiation-protective agentsCarnosineWound healingRadiação ionizanteProtetores contra radiaçãoCarnosinaCicatrização de feridas The purpose of this study was to evaluate the radioprotective effect of carnosine (beta- alanyl-1-histidine) on the wound healing in rats. Therefore, 48 male rats were submitted to a surgical procedure to perform a rectangular wound in the anterior-dorsal region. The animals were divided into 4 experimental groups randomly chosen: control; irradiated; carnosine irradiated and carnosine group. The irradiated and carnosine irradiated group were exposed to a dose (6Gy) of gamma irradiation, in the whole body, 72 hours after surgery. The carnosine and carnosine irradiated groups, in addition to the surgical procedure and the irradiation, received two doses of carnosine aqueous solution, the first one being injected 48 hours after surgery, and the second one 1 hour and 30 minutes before irradiation. The tissue repair of the 4 groups was evaluated at 4, 7, 14, and 21 days after inflicting the wound, by morphological, histochemical and histophysical methods. At all examined periods, it could be observed that the animals from the carnosine irradiated group presented a better developed granulation tissue than the irradiated group and closely similar to that of the control group. Thus, under the experimental conditions used, it was possible to conclude that carnosine is an effective radioprotective substance. O objetivo deste estudo foi avaliar o efeito radioprotetor da carnosina (beta-alanil-1-histidina) no processo de cicatrização em ratos. Para isto, 48 ratos machos foram submetidos a um procedimento cirúrgico para realização de uma ferida retangular na região dorsal anterior. Os animais foram divididos aleatoriamente em 4 grupos experimentais: controle, irradiado, carnosina irradiado e carnosina. Os grupos carnosina e carnosina irradiado foram exposto a uma dose de corpo todo de 6 Gy de radiação gama, 72 horas após a cirurgia para confecção da ferida. O grupo carnosina e carnosina irradiado, adicionalmente, ao procedimento cirúrgico e a irradiação, receberam duas doses de solução aquosa de carnosina, a primeira sendo injetada 48 horas após a cirurgia, e a segunda 1 hora e 30 minutos antes da irradiação. A reparação tecidual nos 4 grupos foi avaliada 4, 7, 14 e 21 dias após a realização da ferida, por métodos morfológicos, histoquímicos e histofísicos. Em todos os períodos examinados, pode ser observado que os animais do grupo carnosina irradiado apresentaram um melhor desenvolvimento do tecido de granulação do que o grupo irradiado e muito similar ao do grupo controle. Logo, de acordo com as condições experimentais usadas, foi possível concluir que a carnosina é uma substância radioprotetora efetiva. Universidade de São Paulo. Faculdade de Odontologia de Bauru2005-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/jaos/article/view/328810.1590/S1678-77572005000300010Journal of Applied Oral Science; Vol. 13 No. 3 (2005); 253-258 Journal of Applied Oral Science; Vol. 13 Núm. 3 (2005); 253-258 Journal of Applied Oral Science; v. 13 n. 3 (2005); 253-258 1678-77651678-7757reponame:Journal of applied oral science (Online)instname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/jaos/article/view/3288/3978Copyright (c) 2005 Journal of Applied Oral Scienceinfo:eu-repo/semantics/openAccessTanaka, Rosana AramakiRamos, Flávia Maria de MoraesAlmeida, Solange Maria deVizioli, Mário RobertoBóscolo, Frab Norberto2012-04-27T11:51:00Zoai:revistas.usp.br:article/3288Revistahttp://www.scielo.br/jaosPUBhttps://www.revistas.usp.br/jaos/oai||jaos@usp.br1678-77651678-7757opendoar:2012-04-27T11:51Journal of applied oral science (Online) - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Avaliação do efeito radioprotetor da carnosina (beta-alanil-1- histidina) no processo de cicatrização em ratos Evaluation of radioprotective effect of carnosine (beta- alanyl-1- histidine) on the wound healing in rats |
title |
Avaliação do efeito radioprotetor da carnosina (beta-alanil-1- histidina) no processo de cicatrização em ratos |
spellingShingle |
Avaliação do efeito radioprotetor da carnosina (beta-alanil-1- histidina) no processo de cicatrização em ratos Tanaka, Rosana Aramaki Radiation ionizing Radiation-protective agents Carnosine Wound healing Radiação ionizante Protetores contra radiação Carnosina Cicatrização de feridas |
title_short |
Avaliação do efeito radioprotetor da carnosina (beta-alanil-1- histidina) no processo de cicatrização em ratos |
title_full |
Avaliação do efeito radioprotetor da carnosina (beta-alanil-1- histidina) no processo de cicatrização em ratos |
title_fullStr |
Avaliação do efeito radioprotetor da carnosina (beta-alanil-1- histidina) no processo de cicatrização em ratos |
title_full_unstemmed |
Avaliação do efeito radioprotetor da carnosina (beta-alanil-1- histidina) no processo de cicatrização em ratos |
title_sort |
Avaliação do efeito radioprotetor da carnosina (beta-alanil-1- histidina) no processo de cicatrização em ratos |
author |
Tanaka, Rosana Aramaki |
author_facet |
Tanaka, Rosana Aramaki Ramos, Flávia Maria de Moraes Almeida, Solange Maria de Vizioli, Mário Roberto Bóscolo, Frab Norberto |
author_role |
author |
author2 |
Ramos, Flávia Maria de Moraes Almeida, Solange Maria de Vizioli, Mário Roberto Bóscolo, Frab Norberto |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Tanaka, Rosana Aramaki Ramos, Flávia Maria de Moraes Almeida, Solange Maria de Vizioli, Mário Roberto Bóscolo, Frab Norberto |
dc.subject.por.fl_str_mv |
Radiation ionizing Radiation-protective agents Carnosine Wound healing Radiação ionizante Protetores contra radiação Carnosina Cicatrização de feridas |
topic |
Radiation ionizing Radiation-protective agents Carnosine Wound healing Radiação ionizante Protetores contra radiação Carnosina Cicatrização de feridas |
description |
The purpose of this study was to evaluate the radioprotective effect of carnosine (beta- alanyl-1-histidine) on the wound healing in rats. Therefore, 48 male rats were submitted to a surgical procedure to perform a rectangular wound in the anterior-dorsal region. The animals were divided into 4 experimental groups randomly chosen: control; irradiated; carnosine irradiated and carnosine group. The irradiated and carnosine irradiated group were exposed to a dose (6Gy) of gamma irradiation, in the whole body, 72 hours after surgery. The carnosine and carnosine irradiated groups, in addition to the surgical procedure and the irradiation, received two doses of carnosine aqueous solution, the first one being injected 48 hours after surgery, and the second one 1 hour and 30 minutes before irradiation. The tissue repair of the 4 groups was evaluated at 4, 7, 14, and 21 days after inflicting the wound, by morphological, histochemical and histophysical methods. At all examined periods, it could be observed that the animals from the carnosine irradiated group presented a better developed granulation tissue than the irradiated group and closely similar to that of the control group. Thus, under the experimental conditions used, it was possible to conclude that carnosine is an effective radioprotective substance. |
publishDate |
2005 |
dc.date.none.fl_str_mv |
2005-09-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/jaos/article/view/3288 10.1590/S1678-77572005000300010 |
url |
https://www.revistas.usp.br/jaos/article/view/3288 |
identifier_str_mv |
10.1590/S1678-77572005000300010 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/jaos/article/view/3288/3978 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2005 Journal of Applied Oral Science info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2005 Journal of Applied Oral Science |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Odontologia de Bauru |
publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Odontologia de Bauru |
dc.source.none.fl_str_mv |
Journal of Applied Oral Science; Vol. 13 No. 3 (2005); 253-258 Journal of Applied Oral Science; Vol. 13 Núm. 3 (2005); 253-258 Journal of Applied Oral Science; v. 13 n. 3 (2005); 253-258 1678-7765 1678-7757 reponame:Journal of applied oral science (Online) instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Journal of applied oral science (Online) |
collection |
Journal of applied oral science (Online) |
repository.name.fl_str_mv |
Journal of applied oral science (Online) - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
||jaos@usp.br |
_version_ |
1800221672083554304 |