Effect of ultrasonic streaming on intra-dentinal disinfection and penetration of calcium hydroxide paste in endodontic treatment

Detalhes bibliográficos
Autor(a) principal: ARIAS, Marcela Paola Castro
Data de Publicação: 2016
Outros Autores: MALIZA, Amanda Garcia Alves, MIDENA, Raquel Zanin, GRAEFF, Márcia Sirlene Zardin, DUARTE, Marco Antonio Húngaro, ANDRADE, Flaviana Bombarda de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Journal of applied oral science (Online)
Texto Completo: https://www.revistas.usp.br/jaos/article/view/125358
Resumo: Objective The antimicrobial effect of ultrasonic agitation of calcium hydroxide (CH) pastes in infected bovine dentin and their penetrability were evaluated using confocal laser scanning microscopy (CLSM) and microbiological culture. Material and Methods Fifty-two bovine teeth were infected with Enterococcus faecalis using a new contamination protocol; then they received CH paste and were divided into groups with or without ultrasound. Ultrasonic agitation was conducted for 1 min with a plain point insert. After 15 d, the CLSM analyzed the viable and dead bacteria with Live and Dead assay. The dentinal wall debris was collected by burs, and the colony forming units (CFU/mL) were counted. The penetrability of the paste inside dentinal tubules was tested using the B-rodamine dye. Results The calcium hydroxide paste showed better results with the use of ultrasonic agitation (p
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spelling Effect of ultrasonic streaming on intra-dentinal disinfection and penetration of calcium hydroxide paste in endodontic treatment Objective The antimicrobial effect of ultrasonic agitation of calcium hydroxide (CH) pastes in infected bovine dentin and their penetrability were evaluated using confocal laser scanning microscopy (CLSM) and microbiological culture. Material and Methods Fifty-two bovine teeth were infected with Enterococcus faecalis using a new contamination protocol; then they received CH paste and were divided into groups with or without ultrasound. Ultrasonic agitation was conducted for 1 min with a plain point insert. After 15 d, the CLSM analyzed the viable and dead bacteria with Live and Dead assay. The dentinal wall debris was collected by burs, and the colony forming units (CFU/mL) were counted. The penetrability of the paste inside dentinal tubules was tested using the B-rodamine dye. Results The calcium hydroxide paste showed better results with the use of ultrasonic agitation (pUniversidade de São Paulo. Faculdade de Odontologia de Bauru2016-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/jaos/article/view/12535810.1590/1678-775720150553Journal of Applied Oral Science; Vol. 24 No. 6 (2016); 575-581Journal of Applied Oral Science; Vol. 24 Núm. 6 (2016); 575-581Journal of Applied Oral Science; v. 24 n. 6 (2016); 575-5811678-77651678-7757reponame:Journal of applied oral science (Online)instname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/jaos/article/view/125358/122381Copyright (c) 2016 Journal of Applied Oral Scienceinfo:eu-repo/semantics/openAccessARIAS, Marcela Paola CastroMALIZA, Amanda Garcia AlvesMIDENA, Raquel ZaninGRAEFF, Márcia Sirlene ZardinDUARTE, Marco Antonio HúngaroANDRADE, Flaviana Bombarda de2017-01-05T19:02:03Zoai:revistas.usp.br:article/125358Revistahttp://www.scielo.br/jaosPUBhttps://www.revistas.usp.br/jaos/oai||jaos@usp.br1678-77651678-7757opendoar:2017-01-05T19:02:03Journal of applied oral science (Online) - Universidade de São Paulo (USP)false
dc.title.none.fl_str_mv Effect of ultrasonic streaming on intra-dentinal disinfection and penetration of calcium hydroxide paste in endodontic treatment
title Effect of ultrasonic streaming on intra-dentinal disinfection and penetration of calcium hydroxide paste in endodontic treatment
spellingShingle Effect of ultrasonic streaming on intra-dentinal disinfection and penetration of calcium hydroxide paste in endodontic treatment
ARIAS, Marcela Paola Castro
title_short Effect of ultrasonic streaming on intra-dentinal disinfection and penetration of calcium hydroxide paste in endodontic treatment
title_full Effect of ultrasonic streaming on intra-dentinal disinfection and penetration of calcium hydroxide paste in endodontic treatment
title_fullStr Effect of ultrasonic streaming on intra-dentinal disinfection and penetration of calcium hydroxide paste in endodontic treatment
title_full_unstemmed Effect of ultrasonic streaming on intra-dentinal disinfection and penetration of calcium hydroxide paste in endodontic treatment
title_sort Effect of ultrasonic streaming on intra-dentinal disinfection and penetration of calcium hydroxide paste in endodontic treatment
author ARIAS, Marcela Paola Castro
author_facet ARIAS, Marcela Paola Castro
MALIZA, Amanda Garcia Alves
MIDENA, Raquel Zanin
GRAEFF, Márcia Sirlene Zardin
DUARTE, Marco Antonio Húngaro
ANDRADE, Flaviana Bombarda de
author_role author
author2 MALIZA, Amanda Garcia Alves
MIDENA, Raquel Zanin
GRAEFF, Márcia Sirlene Zardin
DUARTE, Marco Antonio Húngaro
ANDRADE, Flaviana Bombarda de
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv ARIAS, Marcela Paola Castro
MALIZA, Amanda Garcia Alves
MIDENA, Raquel Zanin
GRAEFF, Márcia Sirlene Zardin
DUARTE, Marco Antonio Húngaro
ANDRADE, Flaviana Bombarda de
description Objective The antimicrobial effect of ultrasonic agitation of calcium hydroxide (CH) pastes in infected bovine dentin and their penetrability were evaluated using confocal laser scanning microscopy (CLSM) and microbiological culture. Material and Methods Fifty-two bovine teeth were infected with Enterococcus faecalis using a new contamination protocol; then they received CH paste and were divided into groups with or without ultrasound. Ultrasonic agitation was conducted for 1 min with a plain point insert. After 15 d, the CLSM analyzed the viable and dead bacteria with Live and Dead assay. The dentinal wall debris was collected by burs, and the colony forming units (CFU/mL) were counted. The penetrability of the paste inside dentinal tubules was tested using the B-rodamine dye. Results The calcium hydroxide paste showed better results with the use of ultrasonic agitation (p
publishDate 2016
dc.date.none.fl_str_mv 2016-12-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/jaos/article/view/125358
10.1590/1678-775720150553
url https://www.revistas.usp.br/jaos/article/view/125358
identifier_str_mv 10.1590/1678-775720150553
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/jaos/article/view/125358/122381
dc.rights.driver.fl_str_mv Copyright (c) 2016 Journal of Applied Oral Science
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2016 Journal of Applied Oral Science
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Odontologia de Bauru
publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Odontologia de Bauru
dc.source.none.fl_str_mv Journal of Applied Oral Science; Vol. 24 No. 6 (2016); 575-581
Journal of Applied Oral Science; Vol. 24 Núm. 6 (2016); 575-581
Journal of Applied Oral Science; v. 24 n. 6 (2016); 575-581
1678-7765
1678-7757
reponame:Journal of applied oral science (Online)
instname:Universidade de São Paulo (USP)
instacron:USP
instname_str Universidade de São Paulo (USP)
instacron_str USP
institution USP
reponame_str Journal of applied oral science (Online)
collection Journal of applied oral science (Online)
repository.name.fl_str_mv Journal of applied oral science (Online) - Universidade de São Paulo (USP)
repository.mail.fl_str_mv ||jaos@usp.br
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