Assessing the association between hypoxia during craniofacial development and oral clefts
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Journal of applied oral science (Online) |
Texto Completo: | https://www.revistas.usp.br/jaos/article/view/201715 |
Resumo: | Objectives: To evaluate the association between hypoxia during embryo development and oral clefts in an animal model, and to evaluate the association between polymorphisms in the HIF-1A gene with oral clefts in human families. Material and Methods: The study with the animal model used zebrafish embryos at 8 hours post-fertilization submitted to 30% and 50% hypoxia for 24 hours. At 5 days post-fertilization, the larvae were fixed. The cartilage structures were stained to evaluate craniofacial phenotypes. The family-based association study included 148 Brazilian nuclear families with oral clefts. The association between the genetic polymorphisms rs2301113 and rs2057482 in HIF-1A with oral clefts was tested. We used real time PCR genotyping approach. ANOVA with Tukey's post-test was used to compare means. The transmission/disequilibrium test was used to analyze the distortion of the inheritance of alleles from parents to their affected offspring. Results: For the hypoxic animal model, the anterior portion of the ethmoid plate presented a gap in the anterior edge, forming a cleft. The hypoxia level was associated with the severity of the phenotype (p<0.0001). For the families, there was no under-transmitted allele among the affected progeny (p>0.05). Conclusion: Hypoxia is involved in the oral cleft etiology, however, polymorphisms in HIF-1A are not associated with oral clefts in humans. |
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Journal of applied oral science (Online) |
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Assessing the association between hypoxia during craniofacial development and oral cleftsGeneGeneticChildrenObjectives: To evaluate the association between hypoxia during embryo development and oral clefts in an animal model, and to evaluate the association between polymorphisms in the HIF-1A gene with oral clefts in human families. Material and Methods: The study with the animal model used zebrafish embryos at 8 hours post-fertilization submitted to 30% and 50% hypoxia for 24 hours. At 5 days post-fertilization, the larvae were fixed. The cartilage structures were stained to evaluate craniofacial phenotypes. The family-based association study included 148 Brazilian nuclear families with oral clefts. The association between the genetic polymorphisms rs2301113 and rs2057482 in HIF-1A with oral clefts was tested. We used real time PCR genotyping approach. ANOVA with Tukey's post-test was used to compare means. The transmission/disequilibrium test was used to analyze the distortion of the inheritance of alleles from parents to their affected offspring. Results: For the hypoxic animal model, the anterior portion of the ethmoid plate presented a gap in the anterior edge, forming a cleft. The hypoxia level was associated with the severity of the phenotype (p<0.0001). For the families, there was no under-transmitted allele among the affected progeny (p>0.05). Conclusion: Hypoxia is involved in the oral cleft etiology, however, polymorphisms in HIF-1A are not associated with oral clefts in humans.Universidade de São Paulo. Faculdade de Odontologia de Bauru2022-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/jaos/article/view/201715Journal of Applied Oral Science; Vol. 26 (2018)Journal of Applied Oral Science; Vol. 26 (2018)Journal of Applied Oral Science; v. 26 (2018)1678-77651678-7757reponame:Journal of applied oral science (Online)instname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/jaos/article/view/201715/185799Copyright (c) 2022 Journal of Applied Oral Sciencehttp://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessKüchler, Erika CalvanoSilva, Lea Assed daNelson-Filho, PauloSabóia, Ticiana M.Rentschler, Angela M.Granjeiro, José MauroOliveira, DrielyTannure, Patricia N. Silva, Raquel Assed daAntunes, Leonardo SantosTsang, MichaelVieira, Alexandre R.2022-09-01T13:30:10Zoai:revistas.usp.br:article/201715Revistahttp://www.scielo.br/jaosPUBhttps://www.revistas.usp.br/jaos/oai||jaos@usp.br1678-77651678-7757opendoar:2022-09-01T13:30:10Journal of applied oral science (Online) - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Assessing the association between hypoxia during craniofacial development and oral clefts |
title |
Assessing the association between hypoxia during craniofacial development and oral clefts |
spellingShingle |
Assessing the association between hypoxia during craniofacial development and oral clefts Küchler, Erika Calvano Gene Genetic Children |
title_short |
Assessing the association between hypoxia during craniofacial development and oral clefts |
title_full |
Assessing the association between hypoxia during craniofacial development and oral clefts |
title_fullStr |
Assessing the association between hypoxia during craniofacial development and oral clefts |
title_full_unstemmed |
Assessing the association between hypoxia during craniofacial development and oral clefts |
title_sort |
Assessing the association between hypoxia during craniofacial development and oral clefts |
author |
Küchler, Erika Calvano |
author_facet |
Küchler, Erika Calvano Silva, Lea Assed da Nelson-Filho, Paulo Sabóia, Ticiana M. Rentschler, Angela M. Granjeiro, José Mauro Oliveira, Driely Tannure, Patricia N. Silva, Raquel Assed da Antunes, Leonardo Santos Tsang, Michael Vieira, Alexandre R. |
author_role |
author |
author2 |
Silva, Lea Assed da Nelson-Filho, Paulo Sabóia, Ticiana M. Rentschler, Angela M. Granjeiro, José Mauro Oliveira, Driely Tannure, Patricia N. Silva, Raquel Assed da Antunes, Leonardo Santos Tsang, Michael Vieira, Alexandre R. |
author2_role |
author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Küchler, Erika Calvano Silva, Lea Assed da Nelson-Filho, Paulo Sabóia, Ticiana M. Rentschler, Angela M. Granjeiro, José Mauro Oliveira, Driely Tannure, Patricia N. Silva, Raquel Assed da Antunes, Leonardo Santos Tsang, Michael Vieira, Alexandre R. |
dc.subject.por.fl_str_mv |
Gene Genetic Children |
topic |
Gene Genetic Children |
description |
Objectives: To evaluate the association between hypoxia during embryo development and oral clefts in an animal model, and to evaluate the association between polymorphisms in the HIF-1A gene with oral clefts in human families. Material and Methods: The study with the animal model used zebrafish embryos at 8 hours post-fertilization submitted to 30% and 50% hypoxia for 24 hours. At 5 days post-fertilization, the larvae were fixed. The cartilage structures were stained to evaluate craniofacial phenotypes. The family-based association study included 148 Brazilian nuclear families with oral clefts. The association between the genetic polymorphisms rs2301113 and rs2057482 in HIF-1A with oral clefts was tested. We used real time PCR genotyping approach. ANOVA with Tukey's post-test was used to compare means. The transmission/disequilibrium test was used to analyze the distortion of the inheritance of alleles from parents to their affected offspring. Results: For the hypoxic animal model, the anterior portion of the ethmoid plate presented a gap in the anterior edge, forming a cleft. The hypoxia level was associated with the severity of the phenotype (p<0.0001). For the families, there was no under-transmitted allele among the affected progeny (p>0.05). Conclusion: Hypoxia is involved in the oral cleft etiology, however, polymorphisms in HIF-1A are not associated with oral clefts in humans. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-09-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/jaos/article/view/201715 |
url |
https://www.revistas.usp.br/jaos/article/view/201715 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/jaos/article/view/201715/185799 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2022 Journal of Applied Oral Science http://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2022 Journal of Applied Oral Science http://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Odontologia de Bauru |
publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Odontologia de Bauru |
dc.source.none.fl_str_mv |
Journal of Applied Oral Science; Vol. 26 (2018) Journal of Applied Oral Science; Vol. 26 (2018) Journal of Applied Oral Science; v. 26 (2018) 1678-7765 1678-7757 reponame:Journal of applied oral science (Online) instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Journal of applied oral science (Online) |
collection |
Journal of applied oral science (Online) |
repository.name.fl_str_mv |
Journal of applied oral science (Online) - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
||jaos@usp.br |
_version_ |
1800221683194265600 |