A model system to study antimicrobial strategies in endodontic biofilms

Detalhes bibliográficos
Autor(a) principal: Estrela,Carlos
Data de Publicação: 2009
Outros Autores: Sydney,Gilson Blitzkow, Figueiredo,José Antonio Poli, Estrela,Cyntia Rodrigues de Araújo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Journal of applied oral science (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572009000200003
Resumo: The purpose of this work was to develop a model system to study antimicrobial strategies in endodontic biofilms. Enterococcus faecalis suspension was colonized in 10 human root canals. Five milliliters of Brain Heart Infusion (BHI) were mixed with 5 mL of the bacterial inoculums (E. faecalis) and inoculated with sufficient volume to fill the root canal during 60 days. This procedure was repeated every 72 h, always using 24-h pure culture prepared and adjusted to No. 1 MacFarland turbidity standard. Biofilm formation was analyzed by scanning electron microscopy (SEM). E. faecalis consistently adhered to collagen structure, colonized dentin surface, progressed towards the dentinal tubules and formed a biofilm. The proposed biofilm model seems to be viable for studies on antimicrobial strategies, and allows for a satisfactory colonization time of selected bacterial species with virulence and adherence properties.
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spelling A model system to study antimicrobial strategies in endodontic biofilmsBiofilmEndodontic biofilmEnterococcus faecalisIntracanal medicamentsAntimicrobial strategiesThe purpose of this work was to develop a model system to study antimicrobial strategies in endodontic biofilms. Enterococcus faecalis suspension was colonized in 10 human root canals. Five milliliters of Brain Heart Infusion (BHI) were mixed with 5 mL of the bacterial inoculums (E. faecalis) and inoculated with sufficient volume to fill the root canal during 60 days. This procedure was repeated every 72 h, always using 24-h pure culture prepared and adjusted to No. 1 MacFarland turbidity standard. Biofilm formation was analyzed by scanning electron microscopy (SEM). E. faecalis consistently adhered to collagen structure, colonized dentin surface, progressed towards the dentinal tubules and formed a biofilm. The proposed biofilm model seems to be viable for studies on antimicrobial strategies, and allows for a satisfactory colonization time of selected bacterial species with virulence and adherence properties.Faculdade De Odontologia De Bauru - USP2009-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572009000200003Journal of Applied Oral Science v.17 n.2 2009reponame:Journal of applied oral science (Online)instname:Universidade de São Paulo (USP)instacron:USP10.1590/S1678-77572009000200003info:eu-repo/semantics/openAccessEstrela,CarlosSydney,Gilson BlitzkowFigueiredo,José Antonio PoliEstrela,Cyntia Rodrigues de Araújoeng2009-01-19T00:00:00Zoai:scielo:S1678-77572009000200003Revistahttp://www.scielo.br/jaosPUBhttps://old.scielo.br/oai/scielo-oai.php||jaos@usp.br1678-77651678-7757opendoar:2009-01-19T00:00Journal of applied oral science (Online) - Universidade de São Paulo (USP)false
dc.title.none.fl_str_mv A model system to study antimicrobial strategies in endodontic biofilms
title A model system to study antimicrobial strategies in endodontic biofilms
spellingShingle A model system to study antimicrobial strategies in endodontic biofilms
Estrela,Carlos
Biofilm
Endodontic biofilm
Enterococcus faecalis
Intracanal medicaments
Antimicrobial strategies
title_short A model system to study antimicrobial strategies in endodontic biofilms
title_full A model system to study antimicrobial strategies in endodontic biofilms
title_fullStr A model system to study antimicrobial strategies in endodontic biofilms
title_full_unstemmed A model system to study antimicrobial strategies in endodontic biofilms
title_sort A model system to study antimicrobial strategies in endodontic biofilms
author Estrela,Carlos
author_facet Estrela,Carlos
Sydney,Gilson Blitzkow
Figueiredo,José Antonio Poli
Estrela,Cyntia Rodrigues de Araújo
author_role author
author2 Sydney,Gilson Blitzkow
Figueiredo,José Antonio Poli
Estrela,Cyntia Rodrigues de Araújo
author2_role author
author
author
dc.contributor.author.fl_str_mv Estrela,Carlos
Sydney,Gilson Blitzkow
Figueiredo,José Antonio Poli
Estrela,Cyntia Rodrigues de Araújo
dc.subject.por.fl_str_mv Biofilm
Endodontic biofilm
Enterococcus faecalis
Intracanal medicaments
Antimicrobial strategies
topic Biofilm
Endodontic biofilm
Enterococcus faecalis
Intracanal medicaments
Antimicrobial strategies
description The purpose of this work was to develop a model system to study antimicrobial strategies in endodontic biofilms. Enterococcus faecalis suspension was colonized in 10 human root canals. Five milliliters of Brain Heart Infusion (BHI) were mixed with 5 mL of the bacterial inoculums (E. faecalis) and inoculated with sufficient volume to fill the root canal during 60 days. This procedure was repeated every 72 h, always using 24-h pure culture prepared and adjusted to No. 1 MacFarland turbidity standard. Biofilm formation was analyzed by scanning electron microscopy (SEM). E. faecalis consistently adhered to collagen structure, colonized dentin surface, progressed towards the dentinal tubules and formed a biofilm. The proposed biofilm model seems to be viable for studies on antimicrobial strategies, and allows for a satisfactory colonization time of selected bacterial species with virulence and adherence properties.
publishDate 2009
dc.date.none.fl_str_mv 2009-04-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572009000200003
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572009000200003
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1678-77572009000200003
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Faculdade De Odontologia De Bauru - USP
publisher.none.fl_str_mv Faculdade De Odontologia De Bauru - USP
dc.source.none.fl_str_mv Journal of Applied Oral Science v.17 n.2 2009
reponame:Journal of applied oral science (Online)
instname:Universidade de São Paulo (USP)
instacron:USP
instname_str Universidade de São Paulo (USP)
instacron_str USP
institution USP
reponame_str Journal of applied oral science (Online)
collection Journal of applied oral science (Online)
repository.name.fl_str_mv Journal of applied oral science (Online) - Universidade de São Paulo (USP)
repository.mail.fl_str_mv ||jaos@usp.br
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