Immunolocalization of markers for bone formation during guided bone regeneration in osteopenic rats
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Journal of applied oral science (Online) |
Texto Completo: | https://www.revistas.usp.br/jaos/article/view/100918 |
Resumo: | Objective The aim of this paper was to evaluate the repair of onlay autogenous bone grafts covered or not covered by an expanded polytetrafluoroethylene (e-PTFE) membrane using immunohistochemistry in rats with induced estrogen deficiency. Material and Methods Eighty female rats were randomly divided into two groups: ovariectomized (OVX) and with a simulation of the surgical procedure (SHAM). Each of these groups was again divided into groups with either placement of an autogenous bone graft alone (BG) or an autogenous bone graft associated with an e-PTFE membrane (BGM). Animals were euthanized on days 0, 7, 21, 45, and 60. The specimens were subjected to immunohistochemistry for bone sialoprotein (BSP), osteonectin (ONC), and osteocalcin (OCC). Results All groups (OVX+BG, OVX+BMG, SHAM+BG, and SHAM+BMG) showed greater bone formation, observed between 7 and 21 days, when BSP and ONC staining were more intense. At the 45-day, the bone graft showed direct bonding to the recipient bed in all specimens. The ONC and OCC showed more expressed in granulation tissue, in the membrane groups, independently of estrogen deficiency. Conclusions The expression of bone forming markers was not negatively influenced by estrogen deficiency. However, the markers could be influenced by the presence of the e-PTFE membrane. |
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oai:revistas.usp.br:article/100918 |
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Journal of applied oral science (Online) |
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Immunolocalization of markers for bone formation during guided bone regeneration in osteopenic rats Objective The aim of this paper was to evaluate the repair of onlay autogenous bone grafts covered or not covered by an expanded polytetrafluoroethylene (e-PTFE) membrane using immunohistochemistry in rats with induced estrogen deficiency. Material and Methods Eighty female rats were randomly divided into two groups: ovariectomized (OVX) and with a simulation of the surgical procedure (SHAM). Each of these groups was again divided into groups with either placement of an autogenous bone graft alone (BG) or an autogenous bone graft associated with an e-PTFE membrane (BGM). Animals were euthanized on days 0, 7, 21, 45, and 60. The specimens were subjected to immunohistochemistry for bone sialoprotein (BSP), osteonectin (ONC), and osteocalcin (OCC). Results All groups (OVX+BG, OVX+BMG, SHAM+BG, and SHAM+BMG) showed greater bone formation, observed between 7 and 21 days, when BSP and ONC staining were more intense. At the 45-day, the bone graft showed direct bonding to the recipient bed in all specimens. The ONC and OCC showed more expressed in granulation tissue, in the membrane groups, independently of estrogen deficiency. Conclusions The expression of bone forming markers was not negatively influenced by estrogen deficiency. However, the markers could be influenced by the presence of the e-PTFE membrane. Universidade de São Paulo. Faculdade de Odontologia de Bauru2014-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/jaos/article/view/10091810.1590/1678-775720140190Journal of Applied Oral Science; Vol. 22 No. 6 (2014); 541-553Journal of Applied Oral Science; Vol. 22 Núm. 6 (2014); 541-553Journal of Applied Oral Science; v. 22 n. 6 (2014); 541-5531678-77651678-7757reponame:Journal of applied oral science (Online)instname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/jaos/article/view/100918/99588Copyright (c) 2014 Journal of Applied Oral Scienceinfo:eu-repo/semantics/openAccessTERA, Tábata de Mello NASCIMENTO, Rodrigo Dias PRADO, Renata Falchete do SANTAMARIA, Mauro Pedrine JARDINI, Maria Aparecida Neves 2015-07-28T17:05:08Zoai:revistas.usp.br:article/100918Revistahttp://www.scielo.br/jaosPUBhttps://www.revistas.usp.br/jaos/oai||jaos@usp.br1678-77651678-7757opendoar:2015-07-28T17:05:08Journal of applied oral science (Online) - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Immunolocalization of markers for bone formation during guided bone regeneration in osteopenic rats |
title |
Immunolocalization of markers for bone formation during guided bone regeneration in osteopenic rats |
spellingShingle |
Immunolocalization of markers for bone formation during guided bone regeneration in osteopenic rats TERA, Tábata de Mello |
title_short |
Immunolocalization of markers for bone formation during guided bone regeneration in osteopenic rats |
title_full |
Immunolocalization of markers for bone formation during guided bone regeneration in osteopenic rats |
title_fullStr |
Immunolocalization of markers for bone formation during guided bone regeneration in osteopenic rats |
title_full_unstemmed |
Immunolocalization of markers for bone formation during guided bone regeneration in osteopenic rats |
title_sort |
Immunolocalization of markers for bone formation during guided bone regeneration in osteopenic rats |
author |
TERA, Tábata de Mello |
author_facet |
TERA, Tábata de Mello NASCIMENTO, Rodrigo Dias PRADO, Renata Falchete do SANTAMARIA, Mauro Pedrine JARDINI, Maria Aparecida Neves |
author_role |
author |
author2 |
NASCIMENTO, Rodrigo Dias PRADO, Renata Falchete do SANTAMARIA, Mauro Pedrine JARDINI, Maria Aparecida Neves |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
TERA, Tábata de Mello NASCIMENTO, Rodrigo Dias PRADO, Renata Falchete do SANTAMARIA, Mauro Pedrine JARDINI, Maria Aparecida Neves |
description |
Objective The aim of this paper was to evaluate the repair of onlay autogenous bone grafts covered or not covered by an expanded polytetrafluoroethylene (e-PTFE) membrane using immunohistochemistry in rats with induced estrogen deficiency. Material and Methods Eighty female rats were randomly divided into two groups: ovariectomized (OVX) and with a simulation of the surgical procedure (SHAM). Each of these groups was again divided into groups with either placement of an autogenous bone graft alone (BG) or an autogenous bone graft associated with an e-PTFE membrane (BGM). Animals were euthanized on days 0, 7, 21, 45, and 60. The specimens were subjected to immunohistochemistry for bone sialoprotein (BSP), osteonectin (ONC), and osteocalcin (OCC). Results All groups (OVX+BG, OVX+BMG, SHAM+BG, and SHAM+BMG) showed greater bone formation, observed between 7 and 21 days, when BSP and ONC staining were more intense. At the 45-day, the bone graft showed direct bonding to the recipient bed in all specimens. The ONC and OCC showed more expressed in granulation tissue, in the membrane groups, independently of estrogen deficiency. Conclusions The expression of bone forming markers was not negatively influenced by estrogen deficiency. However, the markers could be influenced by the presence of the e-PTFE membrane. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-12-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/jaos/article/view/100918 10.1590/1678-775720140190 |
url |
https://www.revistas.usp.br/jaos/article/view/100918 |
identifier_str_mv |
10.1590/1678-775720140190 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/jaos/article/view/100918/99588 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2014 Journal of Applied Oral Science info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2014 Journal of Applied Oral Science |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Odontologia de Bauru |
publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Odontologia de Bauru |
dc.source.none.fl_str_mv |
Journal of Applied Oral Science; Vol. 22 No. 6 (2014); 541-553 Journal of Applied Oral Science; Vol. 22 Núm. 6 (2014); 541-553 Journal of Applied Oral Science; v. 22 n. 6 (2014); 541-553 1678-7765 1678-7757 reponame:Journal of applied oral science (Online) instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Journal of applied oral science (Online) |
collection |
Journal of applied oral science (Online) |
repository.name.fl_str_mv |
Journal of applied oral science (Online) - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
||jaos@usp.br |
_version_ |
1800221678700068864 |