Demetallization of Enterococcus faecalis biofilm: a preliminary study
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Journal of applied oral science (Online) |
Texto Completo: | https://www.revistas.usp.br/jaos/article/view/143544 |
Resumo: | Objectives To determine the concentration of calcium, iron, manganese and zinc ions after the application of chelator to Enterococcus faecalis biofilms. Material and Methods Fifty bovine maxillary central incisors were prepared and inoculated with E. faecalis for 60 days. The following were used as irrigation solutions: 17% EDTA (pH 3, 7 and 10), 2.5% sodium hypochlorite (NaOCl) combined with 17% EDTA (pH 3, 7 and 10), distilled water (pH 3, 7 and 10), and 2.5% NaOCl. Each solution was kept in the root canal for five minutes. Fifteen uncontaminated root canals were irrigated with 17% EDTA (pH 3, 7 and 10). Six teeth were used as bacterial control. The number of calcium, iron, manganese and zinc ions was determined using flame atomic absorption spectrometry. Mean ± standard deviation (SD) values were used for descriptive statistics. Results Calcium chelation using 17% EDTA at pH 7 was higher than at pH 3 and 10, regardless of whether bacterial biofilm was present. The highest concentration of iron occurred at pH 3 in the presence of bacterial biofilm. The highest concentration of manganese found was 2.5% NaOCl and 17% EDTA at pH 7 in the presence of bacterial biofilm. Zinc levels were not detectable. Conclusions The pH of chelating agents affected the removal of calcium, iron, and manganese ions. The concentration of iron ions in root canals with bacterial biofilm was higher after the use of 17% EDTA at pH 3 than after the use of the other solutions at all pH levels. |
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Journal of applied oral science (Online) |
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Demetallization of Enterococcus faecalis biofilm: a preliminary studyBiofilmsEDTASodium hypochloriteEnterococcus faecalisObjectives To determine the concentration of calcium, iron, manganese and zinc ions after the application of chelator to Enterococcus faecalis biofilms. Material and Methods Fifty bovine maxillary central incisors were prepared and inoculated with E. faecalis for 60 days. The following were used as irrigation solutions: 17% EDTA (pH 3, 7 and 10), 2.5% sodium hypochlorite (NaOCl) combined with 17% EDTA (pH 3, 7 and 10), distilled water (pH 3, 7 and 10), and 2.5% NaOCl. Each solution was kept in the root canal for five minutes. Fifteen uncontaminated root canals were irrigated with 17% EDTA (pH 3, 7 and 10). Six teeth were used as bacterial control. The number of calcium, iron, manganese and zinc ions was determined using flame atomic absorption spectrometry. Mean ± standard deviation (SD) values were used for descriptive statistics. Results Calcium chelation using 17% EDTA at pH 7 was higher than at pH 3 and 10, regardless of whether bacterial biofilm was present. The highest concentration of iron occurred at pH 3 in the presence of bacterial biofilm. The highest concentration of manganese found was 2.5% NaOCl and 17% EDTA at pH 7 in the presence of bacterial biofilm. Zinc levels were not detectable. Conclusions The pH of chelating agents affected the removal of calcium, iron, and manganese ions. The concentration of iron ions in root canals with bacterial biofilm was higher after the use of 17% EDTA at pH 3 than after the use of the other solutions at all pH levels.Universidade de São Paulo. Faculdade de Odontologia de Bauru2018-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/jaos/article/view/14354410.1590/1678-7757-2017-0374Journal of Applied Oral Science; Vol. 26 (2018); e20170374Journal of Applied Oral Science; Vol. 26 (2018); e20170374Journal of Applied Oral Science; v. 26 (2018); e201703741678-77651678-7757reponame:Journal of applied oral science (Online)instname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/jaos/article/view/143544/138234Copyright (c) 2018 Journal of Applied Oral Scienceinfo:eu-repo/semantics/openAccessESTRELA, CarlosCOSTA E SILVA, RodrigoURBAN, Roberta CerasiGONÇALVES, Pablo JoséSILVA, Júlio A.ESTRELA, Cyntia R.A.PECORA, Jesus DjalmaPETERS, Ove A.2018-03-20T12:30:07Zoai:revistas.usp.br:article/143544Revistahttp://www.scielo.br/jaosPUBhttps://www.revistas.usp.br/jaos/oai||jaos@usp.br1678-77651678-7757opendoar:2018-03-20T12:30:07Journal of applied oral science (Online) - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Demetallization of Enterococcus faecalis biofilm: a preliminary study |
title |
Demetallization of Enterococcus faecalis biofilm: a preliminary study |
spellingShingle |
Demetallization of Enterococcus faecalis biofilm: a preliminary study ESTRELA, Carlos Biofilms EDTA Sodium hypochlorite Enterococcus faecalis |
title_short |
Demetallization of Enterococcus faecalis biofilm: a preliminary study |
title_full |
Demetallization of Enterococcus faecalis biofilm: a preliminary study |
title_fullStr |
Demetallization of Enterococcus faecalis biofilm: a preliminary study |
title_full_unstemmed |
Demetallization of Enterococcus faecalis biofilm: a preliminary study |
title_sort |
Demetallization of Enterococcus faecalis biofilm: a preliminary study |
author |
ESTRELA, Carlos |
author_facet |
ESTRELA, Carlos COSTA E SILVA, Rodrigo URBAN, Roberta Cerasi GONÇALVES, Pablo José SILVA, Júlio A. ESTRELA, Cyntia R.A. PECORA, Jesus Djalma PETERS, Ove A. |
author_role |
author |
author2 |
COSTA E SILVA, Rodrigo URBAN, Roberta Cerasi GONÇALVES, Pablo José SILVA, Júlio A. ESTRELA, Cyntia R.A. PECORA, Jesus Djalma PETERS, Ove A. |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
ESTRELA, Carlos COSTA E SILVA, Rodrigo URBAN, Roberta Cerasi GONÇALVES, Pablo José SILVA, Júlio A. ESTRELA, Cyntia R.A. PECORA, Jesus Djalma PETERS, Ove A. |
dc.subject.por.fl_str_mv |
Biofilms EDTA Sodium hypochlorite Enterococcus faecalis |
topic |
Biofilms EDTA Sodium hypochlorite Enterococcus faecalis |
description |
Objectives To determine the concentration of calcium, iron, manganese and zinc ions after the application of chelator to Enterococcus faecalis biofilms. Material and Methods Fifty bovine maxillary central incisors were prepared and inoculated with E. faecalis for 60 days. The following were used as irrigation solutions: 17% EDTA (pH 3, 7 and 10), 2.5% sodium hypochlorite (NaOCl) combined with 17% EDTA (pH 3, 7 and 10), distilled water (pH 3, 7 and 10), and 2.5% NaOCl. Each solution was kept in the root canal for five minutes. Fifteen uncontaminated root canals were irrigated with 17% EDTA (pH 3, 7 and 10). Six teeth were used as bacterial control. The number of calcium, iron, manganese and zinc ions was determined using flame atomic absorption spectrometry. Mean ± standard deviation (SD) values were used for descriptive statistics. Results Calcium chelation using 17% EDTA at pH 7 was higher than at pH 3 and 10, regardless of whether bacterial biofilm was present. The highest concentration of iron occurred at pH 3 in the presence of bacterial biofilm. The highest concentration of manganese found was 2.5% NaOCl and 17% EDTA at pH 7 in the presence of bacterial biofilm. Zinc levels were not detectable. Conclusions The pH of chelating agents affected the removal of calcium, iron, and manganese ions. The concentration of iron ions in root canals with bacterial biofilm was higher after the use of 17% EDTA at pH 3 than after the use of the other solutions at all pH levels. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/jaos/article/view/143544 10.1590/1678-7757-2017-0374 |
url |
https://www.revistas.usp.br/jaos/article/view/143544 |
identifier_str_mv |
10.1590/1678-7757-2017-0374 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/jaos/article/view/143544/138234 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2018 Journal of Applied Oral Science info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2018 Journal of Applied Oral Science |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Odontologia de Bauru |
publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Odontologia de Bauru |
dc.source.none.fl_str_mv |
Journal of Applied Oral Science; Vol. 26 (2018); e20170374 Journal of Applied Oral Science; Vol. 26 (2018); e20170374 Journal of Applied Oral Science; v. 26 (2018); e20170374 1678-7765 1678-7757 reponame:Journal of applied oral science (Online) instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Journal of applied oral science (Online) |
collection |
Journal of applied oral science (Online) |
repository.name.fl_str_mv |
Journal of applied oral science (Online) - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
||jaos@usp.br |
_version_ |
1787713195741282304 |