Cytotoxicity evaluation of two root canal sealers and a commercial calcium hydroxide paste on THP1 cell line by Trypan Blue assay

Detalhes bibliográficos
Autor(a) principal: Correa, Gabriela Tavares Bekner
Data de Publicação: 2009
Outros Autores: Veranio, Gabriel Alves Costa, Silva, Licínio Esmaeraldo, Hirata Junior, Raphael, Coil, Jeffry M., Scelza, Miriam F. Zaccaro
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Journal of applied oral science (Online)
Texto Completo: https://www.revistas.usp.br/jaos/article/view/3720
Resumo: OBJECTIVE: The aim of this investigation was to evaluate the cytotoxicity of two brands of root canal sealers, epoxy-resin based and zinc oxide-eugenol based, and one commercial calcium hydroxide paste on a monocyte cell line THP-1. MATERIAL AND METHODS: Undiluted (crude extract) and diluted extracts to 10%, 1%, 0.1%, 0.01%, 0.001% and 0.0001% of the sealers were tested for cytotoxicity to THP-1 cells using the trypan blue assay. Extracts were obtained according to ISO standard. Data were analyzed statistically by the Kruskal-Wallis and Mann-Whitney tests at 5% significance level. RESULTS: Crude extract of AH Plus and Fill Canal killed approximately 90% of THP-1 cells versus 36% of THP-1 cells killed by L&C crude extract (p
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spelling Cytotoxicity evaluation of two root canal sealers and a commercial calcium hydroxide paste on THP1 cell line by Trypan Blue assay AH PlusFill CanalL&CCytotoxicityTrypan blue OBJECTIVE: The aim of this investigation was to evaluate the cytotoxicity of two brands of root canal sealers, epoxy-resin based and zinc oxide-eugenol based, and one commercial calcium hydroxide paste on a monocyte cell line THP-1. MATERIAL AND METHODS: Undiluted (crude extract) and diluted extracts to 10%, 1%, 0.1%, 0.01%, 0.001% and 0.0001% of the sealers were tested for cytotoxicity to THP-1 cells using the trypan blue assay. Extracts were obtained according to ISO standard. Data were analyzed statistically by the Kruskal-Wallis and Mann-Whitney tests at 5% significance level. RESULTS: Crude extract of AH Plus and Fill Canal killed approximately 90% of THP-1 cells versus 36% of THP-1 cells killed by L&C crude extract (pUniversidade de São Paulo. Faculdade de Odontologia de Bauru2009-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/jaos/article/view/372010.1590/S1678-77572009000500020Journal of Applied Oral Science; Vol. 17 No. 5 (2009); 457-461 Journal of Applied Oral Science; Vol. 17 Núm. 5 (2009); 457-461 Journal of Applied Oral Science; v. 17 n. 5 (2009); 457-461 1678-77651678-7757reponame:Journal of applied oral science (Online)instname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/jaos/article/view/3720/4410Copyright (c) 2009 Journal of Applied Oral Scienceinfo:eu-repo/semantics/openAccessCorrea, Gabriela Tavares BeknerVeranio, Gabriel Alves CostaSilva, Licínio EsmaeraldoHirata Junior, RaphaelCoil, Jeffry M.Scelza, Miriam F. Zaccaro2012-04-27T12:06:32Zoai:revistas.usp.br:article/3720Revistahttp://www.scielo.br/jaosPUBhttps://www.revistas.usp.br/jaos/oai||jaos@usp.br1678-77651678-7757opendoar:2012-04-27T12:06:32Journal of applied oral science (Online) - Universidade de São Paulo (USP)false
dc.title.none.fl_str_mv Cytotoxicity evaluation of two root canal sealers and a commercial calcium hydroxide paste on THP1 cell line by Trypan Blue assay
title Cytotoxicity evaluation of two root canal sealers and a commercial calcium hydroxide paste on THP1 cell line by Trypan Blue assay
spellingShingle Cytotoxicity evaluation of two root canal sealers and a commercial calcium hydroxide paste on THP1 cell line by Trypan Blue assay
Correa, Gabriela Tavares Bekner
AH Plus
Fill Canal
L&C
Cytotoxicity
Trypan blue
title_short Cytotoxicity evaluation of two root canal sealers and a commercial calcium hydroxide paste on THP1 cell line by Trypan Blue assay
title_full Cytotoxicity evaluation of two root canal sealers and a commercial calcium hydroxide paste on THP1 cell line by Trypan Blue assay
title_fullStr Cytotoxicity evaluation of two root canal sealers and a commercial calcium hydroxide paste on THP1 cell line by Trypan Blue assay
title_full_unstemmed Cytotoxicity evaluation of two root canal sealers and a commercial calcium hydroxide paste on THP1 cell line by Trypan Blue assay
title_sort Cytotoxicity evaluation of two root canal sealers and a commercial calcium hydroxide paste on THP1 cell line by Trypan Blue assay
author Correa, Gabriela Tavares Bekner
author_facet Correa, Gabriela Tavares Bekner
Veranio, Gabriel Alves Costa
Silva, Licínio Esmaeraldo
Hirata Junior, Raphael
Coil, Jeffry M.
Scelza, Miriam F. Zaccaro
author_role author
author2 Veranio, Gabriel Alves Costa
Silva, Licínio Esmaeraldo
Hirata Junior, Raphael
Coil, Jeffry M.
Scelza, Miriam F. Zaccaro
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Correa, Gabriela Tavares Bekner
Veranio, Gabriel Alves Costa
Silva, Licínio Esmaeraldo
Hirata Junior, Raphael
Coil, Jeffry M.
Scelza, Miriam F. Zaccaro
dc.subject.por.fl_str_mv AH Plus
Fill Canal
L&C
Cytotoxicity
Trypan blue
topic AH Plus
Fill Canal
L&C
Cytotoxicity
Trypan blue
description OBJECTIVE: The aim of this investigation was to evaluate the cytotoxicity of two brands of root canal sealers, epoxy-resin based and zinc oxide-eugenol based, and one commercial calcium hydroxide paste on a monocyte cell line THP-1. MATERIAL AND METHODS: Undiluted (crude extract) and diluted extracts to 10%, 1%, 0.1%, 0.01%, 0.001% and 0.0001% of the sealers were tested for cytotoxicity to THP-1 cells using the trypan blue assay. Extracts were obtained according to ISO standard. Data were analyzed statistically by the Kruskal-Wallis and Mann-Whitney tests at 5% significance level. RESULTS: Crude extract of AH Plus and Fill Canal killed approximately 90% of THP-1 cells versus 36% of THP-1 cells killed by L&C crude extract (p
publishDate 2009
dc.date.none.fl_str_mv 2009-10-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/jaos/article/view/3720
10.1590/S1678-77572009000500020
url https://www.revistas.usp.br/jaos/article/view/3720
identifier_str_mv 10.1590/S1678-77572009000500020
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/jaos/article/view/3720/4410
dc.rights.driver.fl_str_mv Copyright (c) 2009 Journal of Applied Oral Science
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2009 Journal of Applied Oral Science
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Odontologia de Bauru
publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Odontologia de Bauru
dc.source.none.fl_str_mv Journal of Applied Oral Science; Vol. 17 No. 5 (2009); 457-461
Journal of Applied Oral Science; Vol. 17 Núm. 5 (2009); 457-461
Journal of Applied Oral Science; v. 17 n. 5 (2009); 457-461
1678-7765
1678-7757
reponame:Journal of applied oral science (Online)
instname:Universidade de São Paulo (USP)
instacron:USP
instname_str Universidade de São Paulo (USP)
instacron_str USP
institution USP
reponame_str Journal of applied oral science (Online)
collection Journal of applied oral science (Online)
repository.name.fl_str_mv Journal of applied oral science (Online) - Universidade de São Paulo (USP)
repository.mail.fl_str_mv ||jaos@usp.br
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