Histomicrobiologic aspects of the root canal system and periapical lesions in dogs' teeth after rotary instrumentation and intracanal dressing with Ca(OH)2 pastes
Autor(a) principal: | |
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Data de Publicação: | 2006 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Journal of applied oral science (Online) |
Texto Completo: | https://www.revistas.usp.br/jaos/article/view/3400 |
Resumo: | OBJECTIVE: The purpose of this study was to evaluate the distribution of microorganisms in the root canal system (RCS) and periapical lesions of dogs' teeth after rotary instrumentation and placement of different calcium hydroxide [Ca(OH)2]-based intracanal dressings. MATERIALS AND METHODS: Chronic periapical lesions were experimentally induced in 80 premolar roots of four dogs. Instrumentation was undertaken using the ProFile rotary system and irrigation with 5.25% sodium hypochlorite. The following Ca(OH)2-based pastes were applied for 21 days: group 1 - Calen (n=18); group 2 - Calen+CPMC (n=20); group 3 - Ca(OH)2 p.a. + anaesthetic solution (n=16) and group 4 - Ca(OH)2 p.a.+ 2% chlorhexidine digluconate (n=18). Eight root canals without endodontic treatment constituted the control group. Histological sections were obtained and stained with Brown & Brenn staining technique to evaluate the presence of microorganisms in the main root canal, ramifications of the apical delta and secondary canals, apical cementoplasts, dentinal tubules, areas of cemental resorption and periapical lesions. The results were analyzed statistically by the Mann-Whitney U test (p;0.05). The percentage of RCS sites containing microorganisms in groups 1, 2, 3, 4 and control were: 67.6%, 62.5%, 78.2%, 62.0% and 87.6%, respectively. CONCLUSION: In conclusion, the histomicrobiological analysis showed that the rotary instrumentation and the different calcium hydroxide pastes employed did not effectively eliminate the infection from the RCS and periapical lesions. However, several bacteria seen in the histological sections were probably dead or were inactivated by the biomechanical preparation and calcium hydroxide-based intracanal dressing. |
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Journal of applied oral science (Online) |
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Histomicrobiologic aspects of the root canal system and periapical lesions in dogs' teeth after rotary instrumentation and intracanal dressing with Ca(OH)2 pastes Aspectos histomicrobiológicos do sistema de canais radiculares e das lesões periapicais em dentes de cães após instrumentação automatizada e medicação intracanal com pastas de Ca(OH)2 Pastas de hidróxido de cálcioLesão periapicalTratamento dos canais radicularesCalcium hydroxide pastesEndodontic infectionPeriapical lesionsRoot canal therapy OBJECTIVE: The purpose of this study was to evaluate the distribution of microorganisms in the root canal system (RCS) and periapical lesions of dogs' teeth after rotary instrumentation and placement of different calcium hydroxide [Ca(OH)2]-based intracanal dressings. MATERIALS AND METHODS: Chronic periapical lesions were experimentally induced in 80 premolar roots of four dogs. Instrumentation was undertaken using the ProFile rotary system and irrigation with 5.25% sodium hypochlorite. The following Ca(OH)2-based pastes were applied for 21 days: group 1 - Calen (n=18); group 2 - Calen+CPMC (n=20); group 3 - Ca(OH)2 p.a. + anaesthetic solution (n=16) and group 4 - Ca(OH)2 p.a.+ 2% chlorhexidine digluconate (n=18). Eight root canals without endodontic treatment constituted the control group. Histological sections were obtained and stained with Brown & Brenn staining technique to evaluate the presence of microorganisms in the main root canal, ramifications of the apical delta and secondary canals, apical cementoplasts, dentinal tubules, areas of cemental resorption and periapical lesions. The results were analyzed statistically by the Mann-Whitney U test (p;0.05). The percentage of RCS sites containing microorganisms in groups 1, 2, 3, 4 and control were: 67.6%, 62.5%, 78.2%, 62.0% and 87.6%, respectively. CONCLUSION: In conclusion, the histomicrobiological analysis showed that the rotary instrumentation and the different calcium hydroxide pastes employed did not effectively eliminate the infection from the RCS and periapical lesions. However, several bacteria seen in the histological sections were probably dead or were inactivated by the biomechanical preparation and calcium hydroxide-based intracanal dressing. OBJETIVO: Neste estudo avaliou-se a distribuição dos microrganismos no sistema de canais radiculares (SCR) e região periapical (RP) de cães após instrumentação automatizada e utilização de pastas à base de hidróxido de cálcio CaO(H)2. MATERIAL E MÉTODOS: Lesões periapicais crônicas foram induzidas em 80 raízes de pré-molares de quatro cães. Após a instrumentação com o sistema Profile auxiliado pela solução de hipoclorito de sódio a 5,25%, aplicou-se as seguintes pastas contendo Ca(OH)2: grupo 1-Calen (n=18), grupo 2-Calen/PMCC (n=20), grupo 3-hidróxido de cálcio p.a. + solução anestésica (n=16) e grupo 4-hidróxido de cálcio p.a. + solução de digluconato de clorexidina a 2% (n=18), por 21 dias. Oito canais radiculares sem tratamento endodôntico constituíram o grupo-controle. Nas secções histológicas coradas por Brown & Brenn, avaliou-se a presença de microrganismos nos 3mm apicais do SCR e RP, incluindo o canal radicular principal, deltas, cementoplastos, túbulos dentinários, reabsorções cementárias e lesão periapical. Os resultados foram analisados pelo teste de Mann-Whitney (p;0.05). Nos grupos 1, 2, 3, 4 e controle, os percentuais de sítios do SCR contendo microrganismos foram: 67.6%, 62.5%, 78.2%, 62.0% e 87.6%, respectivamente. CONCLUSÃO: Histomicrobiologicamente, os protocolos de anti-sepsia utilizados proporcionaram insignificativa redução dos microrganismos no SCR e na RP, embora, metabolicamente, muitas bactérias vistas nas secções histológicas podem ter sido inativadas irreversivelmente ou mesmo perdido a viabilidade em função dos procedimentos de anti-sepsia utilizados. Universidade de São Paulo. Faculdade de Odontologia de Bauru2006-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/jaos/article/view/340010.1590/S1678-77572006000500011Journal of Applied Oral Science; Vol. 14 No. 5 (2006); 355-364 Journal of Applied Oral Science; Vol. 14 Núm. 5 (2006); 355-364 Journal of Applied Oral Science; v. 14 n. 5 (2006); 355-364 1678-77651678-7757reponame:Journal of applied oral science (Online)instname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/jaos/article/view/3400/4090Copyright (c) 2006 Journal of Applied Oral Scienceinfo:eu-repo/semantics/openAccessSoares, Janir AlvesLeonardo, Mário RobertoSilva, Léa Assed Bezerra daTanomaru Filho, MárioIto, Izabel Yoko2012-04-27T11:55:11Zoai:revistas.usp.br:article/3400Revistahttp://www.scielo.br/jaosPUBhttps://www.revistas.usp.br/jaos/oai||jaos@usp.br1678-77651678-7757opendoar:2012-04-27T11:55:11Journal of applied oral science (Online) - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Histomicrobiologic aspects of the root canal system and periapical lesions in dogs' teeth after rotary instrumentation and intracanal dressing with Ca(OH)2 pastes Aspectos histomicrobiológicos do sistema de canais radiculares e das lesões periapicais em dentes de cães após instrumentação automatizada e medicação intracanal com pastas de Ca(OH)2 |
title |
Histomicrobiologic aspects of the root canal system and periapical lesions in dogs' teeth after rotary instrumentation and intracanal dressing with Ca(OH)2 pastes |
spellingShingle |
Histomicrobiologic aspects of the root canal system and periapical lesions in dogs' teeth after rotary instrumentation and intracanal dressing with Ca(OH)2 pastes Soares, Janir Alves Pastas de hidróxido de cálcio Lesão periapical Tratamento dos canais radiculares Calcium hydroxide pastes Endodontic infection Periapical lesions Root canal therapy |
title_short |
Histomicrobiologic aspects of the root canal system and periapical lesions in dogs' teeth after rotary instrumentation and intracanal dressing with Ca(OH)2 pastes |
title_full |
Histomicrobiologic aspects of the root canal system and periapical lesions in dogs' teeth after rotary instrumentation and intracanal dressing with Ca(OH)2 pastes |
title_fullStr |
Histomicrobiologic aspects of the root canal system and periapical lesions in dogs' teeth after rotary instrumentation and intracanal dressing with Ca(OH)2 pastes |
title_full_unstemmed |
Histomicrobiologic aspects of the root canal system and periapical lesions in dogs' teeth after rotary instrumentation and intracanal dressing with Ca(OH)2 pastes |
title_sort |
Histomicrobiologic aspects of the root canal system and periapical lesions in dogs' teeth after rotary instrumentation and intracanal dressing with Ca(OH)2 pastes |
author |
Soares, Janir Alves |
author_facet |
Soares, Janir Alves Leonardo, Mário Roberto Silva, Léa Assed Bezerra da Tanomaru Filho, Mário Ito, Izabel Yoko |
author_role |
author |
author2 |
Leonardo, Mário Roberto Silva, Léa Assed Bezerra da Tanomaru Filho, Mário Ito, Izabel Yoko |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Soares, Janir Alves Leonardo, Mário Roberto Silva, Léa Assed Bezerra da Tanomaru Filho, Mário Ito, Izabel Yoko |
dc.subject.por.fl_str_mv |
Pastas de hidróxido de cálcio Lesão periapical Tratamento dos canais radiculares Calcium hydroxide pastes Endodontic infection Periapical lesions Root canal therapy |
topic |
Pastas de hidróxido de cálcio Lesão periapical Tratamento dos canais radiculares Calcium hydroxide pastes Endodontic infection Periapical lesions Root canal therapy |
description |
OBJECTIVE: The purpose of this study was to evaluate the distribution of microorganisms in the root canal system (RCS) and periapical lesions of dogs' teeth after rotary instrumentation and placement of different calcium hydroxide [Ca(OH)2]-based intracanal dressings. MATERIALS AND METHODS: Chronic periapical lesions were experimentally induced in 80 premolar roots of four dogs. Instrumentation was undertaken using the ProFile rotary system and irrigation with 5.25% sodium hypochlorite. The following Ca(OH)2-based pastes were applied for 21 days: group 1 - Calen (n=18); group 2 - Calen+CPMC (n=20); group 3 - Ca(OH)2 p.a. + anaesthetic solution (n=16) and group 4 - Ca(OH)2 p.a.+ 2% chlorhexidine digluconate (n=18). Eight root canals without endodontic treatment constituted the control group. Histological sections were obtained and stained with Brown & Brenn staining technique to evaluate the presence of microorganisms in the main root canal, ramifications of the apical delta and secondary canals, apical cementoplasts, dentinal tubules, areas of cemental resorption and periapical lesions. The results were analyzed statistically by the Mann-Whitney U test (p;0.05). The percentage of RCS sites containing microorganisms in groups 1, 2, 3, 4 and control were: 67.6%, 62.5%, 78.2%, 62.0% and 87.6%, respectively. CONCLUSION: In conclusion, the histomicrobiological analysis showed that the rotary instrumentation and the different calcium hydroxide pastes employed did not effectively eliminate the infection from the RCS and periapical lesions. However, several bacteria seen in the histological sections were probably dead or were inactivated by the biomechanical preparation and calcium hydroxide-based intracanal dressing. |
publishDate |
2006 |
dc.date.none.fl_str_mv |
2006-10-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/jaos/article/view/3400 10.1590/S1678-77572006000500011 |
url |
https://www.revistas.usp.br/jaos/article/view/3400 |
identifier_str_mv |
10.1590/S1678-77572006000500011 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/jaos/article/view/3400/4090 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2006 Journal of Applied Oral Science info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2006 Journal of Applied Oral Science |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Odontologia de Bauru |
publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Odontologia de Bauru |
dc.source.none.fl_str_mv |
Journal of Applied Oral Science; Vol. 14 No. 5 (2006); 355-364 Journal of Applied Oral Science; Vol. 14 Núm. 5 (2006); 355-364 Journal of Applied Oral Science; v. 14 n. 5 (2006); 355-364 1678-7765 1678-7757 reponame:Journal of applied oral science (Online) instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Journal of applied oral science (Online) |
collection |
Journal of applied oral science (Online) |
repository.name.fl_str_mv |
Journal of applied oral science (Online) - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
||jaos@usp.br |
_version_ |
1800221672818606080 |