In vivo microbiological evaluation of the effect of biomechanical preparation of root canals using different irrigating solutions
Autor(a) principal: | |
---|---|
Data de Publicação: | 2006 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Journal of applied oral science (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572006000200008 |
Resumo: | The aim of this study was to evaluate the antimicrobial effect of biomechanical preparation using different irrigating solutions. Seventy-eight root canals from premolars of four dogs were used. After experimental induction of periapical lesions, the root canals were prepared using the following solutions for irrigation: Group 1) 2.5% sodium hypochlorite (NaOCl); Group 2) 2% chlorhexidine (CHX); Group 3) saline solution and Group 4) control group with no biomechanical preparation. The microbiological evaluation of the root canals was performed by counting the colony forming units (CFUs) using different culture mediums. Two absorbent paper cones were used in each root canal in order to collect the microbiological samples before, and thirty days after the biomechanical preparation. The culture plates were incubated in aerobic, anaerobic and microaerophilic environment. Statistical evaluation was carried out using analysis of variance, Tukey and Student tests. The results demonstrated that there was reduction in the number of microorganisms in the NaOCl and CHX groups (p<0.05). There was greater effectiveness in the chlorhexidine group. The group that used saline solution and the control group presented an increased number of microorganisms. It can be concluded that the use of antimicrobial irrigating solutions during biomechanical preparation promotes the reduction of endodontic microbiota. However, a considerable number of microorganisms were still observed. |
id |
USP-17_b944dc8205515f80aa555e9dedfa963c |
---|---|
oai_identifier_str |
oai:scielo:S1678-77572006000200008 |
network_acronym_str |
USP-17 |
network_name_str |
Journal of applied oral science (Online) |
repository_id_str |
|
spelling |
In vivo microbiological evaluation of the effect of biomechanical preparation of root canals using different irrigating solutionsEndodonticsMicrobiologySodium hypochloriteChlorhexidineThe aim of this study was to evaluate the antimicrobial effect of biomechanical preparation using different irrigating solutions. Seventy-eight root canals from premolars of four dogs were used. After experimental induction of periapical lesions, the root canals were prepared using the following solutions for irrigation: Group 1) 2.5% sodium hypochlorite (NaOCl); Group 2) 2% chlorhexidine (CHX); Group 3) saline solution and Group 4) control group with no biomechanical preparation. The microbiological evaluation of the root canals was performed by counting the colony forming units (CFUs) using different culture mediums. Two absorbent paper cones were used in each root canal in order to collect the microbiological samples before, and thirty days after the biomechanical preparation. The culture plates were incubated in aerobic, anaerobic and microaerophilic environment. Statistical evaluation was carried out using analysis of variance, Tukey and Student tests. The results demonstrated that there was reduction in the number of microorganisms in the NaOCl and CHX groups (p<0.05). There was greater effectiveness in the chlorhexidine group. The group that used saline solution and the control group presented an increased number of microorganisms. It can be concluded that the use of antimicrobial irrigating solutions during biomechanical preparation promotes the reduction of endodontic microbiota. However, a considerable number of microorganisms were still observed.Faculdade De Odontologia De Bauru - USP2006-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572006000200008Journal of Applied Oral Science v.14 n.2 2006reponame:Journal of applied oral science (Online)instname:Universidade de São Paulo (USP)instacron:USP10.1590/S1678-77572006000200008info:eu-repo/semantics/openAccessTanomaru Filho,MárioYamashita,José CarlosLeonardo,Mario RobertoSilva,Léa Assed Bezerra daTanomaru,Juliane Maria GuerreiroIto,Izabel Yokoeng2006-06-08T00:00:00Zoai:scielo:S1678-77572006000200008Revistahttp://www.scielo.br/jaosPUBhttps://old.scielo.br/oai/scielo-oai.php||jaos@usp.br1678-77651678-7757opendoar:2006-06-08T00:00Journal of applied oral science (Online) - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
In vivo microbiological evaluation of the effect of biomechanical preparation of root canals using different irrigating solutions |
title |
In vivo microbiological evaluation of the effect of biomechanical preparation of root canals using different irrigating solutions |
spellingShingle |
In vivo microbiological evaluation of the effect of biomechanical preparation of root canals using different irrigating solutions Tanomaru Filho,Mário Endodontics Microbiology Sodium hypochlorite Chlorhexidine |
title_short |
In vivo microbiological evaluation of the effect of biomechanical preparation of root canals using different irrigating solutions |
title_full |
In vivo microbiological evaluation of the effect of biomechanical preparation of root canals using different irrigating solutions |
title_fullStr |
In vivo microbiological evaluation of the effect of biomechanical preparation of root canals using different irrigating solutions |
title_full_unstemmed |
In vivo microbiological evaluation of the effect of biomechanical preparation of root canals using different irrigating solutions |
title_sort |
In vivo microbiological evaluation of the effect of biomechanical preparation of root canals using different irrigating solutions |
author |
Tanomaru Filho,Mário |
author_facet |
Tanomaru Filho,Mário Yamashita,José Carlos Leonardo,Mario Roberto Silva,Léa Assed Bezerra da Tanomaru,Juliane Maria Guerreiro Ito,Izabel Yoko |
author_role |
author |
author2 |
Yamashita,José Carlos Leonardo,Mario Roberto Silva,Léa Assed Bezerra da Tanomaru,Juliane Maria Guerreiro Ito,Izabel Yoko |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Tanomaru Filho,Mário Yamashita,José Carlos Leonardo,Mario Roberto Silva,Léa Assed Bezerra da Tanomaru,Juliane Maria Guerreiro Ito,Izabel Yoko |
dc.subject.por.fl_str_mv |
Endodontics Microbiology Sodium hypochlorite Chlorhexidine |
topic |
Endodontics Microbiology Sodium hypochlorite Chlorhexidine |
description |
The aim of this study was to evaluate the antimicrobial effect of biomechanical preparation using different irrigating solutions. Seventy-eight root canals from premolars of four dogs were used. After experimental induction of periapical lesions, the root canals were prepared using the following solutions for irrigation: Group 1) 2.5% sodium hypochlorite (NaOCl); Group 2) 2% chlorhexidine (CHX); Group 3) saline solution and Group 4) control group with no biomechanical preparation. The microbiological evaluation of the root canals was performed by counting the colony forming units (CFUs) using different culture mediums. Two absorbent paper cones were used in each root canal in order to collect the microbiological samples before, and thirty days after the biomechanical preparation. The culture plates were incubated in aerobic, anaerobic and microaerophilic environment. Statistical evaluation was carried out using analysis of variance, Tukey and Student tests. The results demonstrated that there was reduction in the number of microorganisms in the NaOCl and CHX groups (p<0.05). There was greater effectiveness in the chlorhexidine group. The group that used saline solution and the control group presented an increased number of microorganisms. It can be concluded that the use of antimicrobial irrigating solutions during biomechanical preparation promotes the reduction of endodontic microbiota. However, a considerable number of microorganisms were still observed. |
publishDate |
2006 |
dc.date.none.fl_str_mv |
2006-04-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572006000200008 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572006000200008 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1678-77572006000200008 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Faculdade De Odontologia De Bauru - USP |
publisher.none.fl_str_mv |
Faculdade De Odontologia De Bauru - USP |
dc.source.none.fl_str_mv |
Journal of Applied Oral Science v.14 n.2 2006 reponame:Journal of applied oral science (Online) instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Journal of applied oral science (Online) |
collection |
Journal of applied oral science (Online) |
repository.name.fl_str_mv |
Journal of applied oral science (Online) - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
||jaos@usp.br |
_version_ |
1748936434258018304 |