Genetic characterization of Xylella fastidiosa isolated from citrus and coffee plants
Autor(a) principal: | |
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Data de Publicação: | 2007 |
Outros Autores: | , , |
Tipo de documento: | Relatório |
Idioma: | eng |
Título da fonte: | Scientia Agrícola (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162007000500005 |
Resumo: | The Citrus Variegated Chlorosis and the Coffee Leaf Scorch are some of the many destructive diseases caused by Xylella fastidiosa, a gram-negative bacterium limited to the xylem of affected plants. As its genetic characterization is still not well established, different isolates of X. fastidiosa from citrus and coffee were evaluated through RAPD (Random Amplified Polymorphic DNA) technique to characterize and classify these isolates based on similarity coefficients. Sixteen isolates of X. fastidiosa were used on this trial, obtained from citrus, coffee and almond. The genetic polymorphism evaluation was performed using six arbitrary 10-base primer pairs. It was possible to establish a dendogram in which the isolates were classified into five groups (A, B, C, D and E). A prevalence of citrus isolates in groups A and D was observed. In groups B and C, there was a prevalence of coffee isolates meanwhile the group D consisted of the almond isolate, solely. |
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Genetic characterization of Xylella fastidiosa isolated from citrus and coffee plantsRAPDgram-negative bacteriumgenetic divergenceThe Citrus Variegated Chlorosis and the Coffee Leaf Scorch are some of the many destructive diseases caused by Xylella fastidiosa, a gram-negative bacterium limited to the xylem of affected plants. As its genetic characterization is still not well established, different isolates of X. fastidiosa from citrus and coffee were evaluated through RAPD (Random Amplified Polymorphic DNA) technique to characterize and classify these isolates based on similarity coefficients. Sixteen isolates of X. fastidiosa were used on this trial, obtained from citrus, coffee and almond. The genetic polymorphism evaluation was performed using six arbitrary 10-base primer pairs. It was possible to establish a dendogram in which the isolates were classified into five groups (A, B, C, D and E). A prevalence of citrus isolates in groups A and D was observed. In groups B and C, there was a prevalence of coffee isolates meanwhile the group D consisted of the almond isolate, solely.Escola Superior de Agricultura "Luiz de Queiroz"2007-10-01info:eu-repo/semantics/reportinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162007000500005Scientia Agricola v.64 n.5 2007reponame:Scientia Agrícola (Online)instname:Universidade de São Paulo (USP)instacron:USP10.1590/S0103-90162007000500005info:eu-repo/semantics/openAccessMiranda,Vicente SavonittiFarias,Paulo Roberto SilvaRoberto,Sérgio RufoLacava,Pedro Magalhãeseng2007-10-05T00:00:00Zoai:scielo:S0103-90162007000500005Revistahttp://revistas.usp.br/sa/indexPUBhttps://old.scielo.br/oai/scielo-oai.phpscientia@usp.br||alleoni@usp.br1678-992X0103-9016opendoar:2007-10-05T00:00Scientia Agrícola (Online) - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Genetic characterization of Xylella fastidiosa isolated from citrus and coffee plants |
title |
Genetic characterization of Xylella fastidiosa isolated from citrus and coffee plants |
spellingShingle |
Genetic characterization of Xylella fastidiosa isolated from citrus and coffee plants Miranda,Vicente Savonitti RAPD gram-negative bacterium genetic divergence |
title_short |
Genetic characterization of Xylella fastidiosa isolated from citrus and coffee plants |
title_full |
Genetic characterization of Xylella fastidiosa isolated from citrus and coffee plants |
title_fullStr |
Genetic characterization of Xylella fastidiosa isolated from citrus and coffee plants |
title_full_unstemmed |
Genetic characterization of Xylella fastidiosa isolated from citrus and coffee plants |
title_sort |
Genetic characterization of Xylella fastidiosa isolated from citrus and coffee plants |
author |
Miranda,Vicente Savonitti |
author_facet |
Miranda,Vicente Savonitti Farias,Paulo Roberto Silva Roberto,Sérgio Rufo Lacava,Pedro Magalhães |
author_role |
author |
author2 |
Farias,Paulo Roberto Silva Roberto,Sérgio Rufo Lacava,Pedro Magalhães |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Miranda,Vicente Savonitti Farias,Paulo Roberto Silva Roberto,Sérgio Rufo Lacava,Pedro Magalhães |
dc.subject.por.fl_str_mv |
RAPD gram-negative bacterium genetic divergence |
topic |
RAPD gram-negative bacterium genetic divergence |
description |
The Citrus Variegated Chlorosis and the Coffee Leaf Scorch are some of the many destructive diseases caused by Xylella fastidiosa, a gram-negative bacterium limited to the xylem of affected plants. As its genetic characterization is still not well established, different isolates of X. fastidiosa from citrus and coffee were evaluated through RAPD (Random Amplified Polymorphic DNA) technique to characterize and classify these isolates based on similarity coefficients. Sixteen isolates of X. fastidiosa were used on this trial, obtained from citrus, coffee and almond. The genetic polymorphism evaluation was performed using six arbitrary 10-base primer pairs. It was possible to establish a dendogram in which the isolates were classified into five groups (A, B, C, D and E). A prevalence of citrus isolates in groups A and D was observed. In groups B and C, there was a prevalence of coffee isolates meanwhile the group D consisted of the almond isolate, solely. |
publishDate |
2007 |
dc.date.none.fl_str_mv |
2007-10-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/report |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
report |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162007000500005 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162007000500005 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0103-90162007000500005 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Escola Superior de Agricultura "Luiz de Queiroz" |
publisher.none.fl_str_mv |
Escola Superior de Agricultura "Luiz de Queiroz" |
dc.source.none.fl_str_mv |
Scientia Agricola v.64 n.5 2007 reponame:Scientia Agrícola (Online) instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Scientia Agrícola (Online) |
collection |
Scientia Agrícola (Online) |
repository.name.fl_str_mv |
Scientia Agrícola (Online) - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
scientia@usp.br||alleoni@usp.br |
_version_ |
1748936460591955968 |