Identification of a non-host plant of Xylella fastidiosa to rear healthy sharpshooter vectors
Autor(a) principal: | |
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Data de Publicação: | 2003 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Scientia Agrícola (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162003000400010 |
Resumo: | Rearing leafhopper (Hemiptera: Cicadellidae) vectors free of Xylella fastidiosa is a requirement for studies of various aspects of vector-pathogen interactions. The selection of a plant that allows vector development but not bacterial multiplication is desirable to produce healthy vectors. In this study, two leafhopper hosts, Vernonia condensata ('boldo') and Aloysia virgata ('lixeira') were needle inoculated with citrus and coffee strains of X. fastidiosa to evaluate if these plants support pathogen colonization. The inoculated plants did not present symptoms and the pathogen was not detected by culture and PCR tests, neither soon after inoculation (7-14 days) nor later, at 1, 4, 6 and 12 months after inoculation. To obtain healthy adults of the leafhopper vectors Acrogonia citrina, Bucephalogonia xanthophis, Dilobopterus costalimai, Homalodisca ignorata and Oncometopia facialis, early-instar nymphs were reared on V. condensata. X. fastidiosa was not detected in any of 175 adults obtained. V. condensata and A. virgata are nonpropagative hosts of X. fastidiosa and enable the production of healthy leafhoppers for vector studies. |
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Identification of a non-host plant of Xylella fastidiosa to rear healthy sharpshooter vectorsVernonia condensatacitrus variegated chlorosiscoffee leaf scorchsharpshooter vectorsrearing techniqueRearing leafhopper (Hemiptera: Cicadellidae) vectors free of Xylella fastidiosa is a requirement for studies of various aspects of vector-pathogen interactions. The selection of a plant that allows vector development but not bacterial multiplication is desirable to produce healthy vectors. In this study, two leafhopper hosts, Vernonia condensata ('boldo') and Aloysia virgata ('lixeira') were needle inoculated with citrus and coffee strains of X. fastidiosa to evaluate if these plants support pathogen colonization. The inoculated plants did not present symptoms and the pathogen was not detected by culture and PCR tests, neither soon after inoculation (7-14 days) nor later, at 1, 4, 6 and 12 months after inoculation. To obtain healthy adults of the leafhopper vectors Acrogonia citrina, Bucephalogonia xanthophis, Dilobopterus costalimai, Homalodisca ignorata and Oncometopia facialis, early-instar nymphs were reared on V. condensata. X. fastidiosa was not detected in any of 175 adults obtained. V. condensata and A. virgata are nonpropagative hosts of X. fastidiosa and enable the production of healthy leafhoppers for vector studies.Escola Superior de Agricultura "Luiz de Queiroz"2003-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162003000400010Scientia Agricola v.60 n.4 2003reponame:Scientia Agrícola (Online)instname:Universidade de São Paulo (USP)instacron:USP10.1590/S0103-90162003000400010info:eu-repo/semantics/openAccessMarucci,Rosangela CristinaGiustolin,Teresinha AugustaMiranda,Marcelo Pedreira deMiquelote,HelenAlmeida,Rodrigo Piacentini Paes deLopes,João Roberto Spottieng2003-11-05T00:00:00Zoai:scielo:S0103-90162003000400010Revistahttp://revistas.usp.br/sa/indexPUBhttps://old.scielo.br/oai/scielo-oai.phpscientia@usp.br||alleoni@usp.br1678-992X0103-9016opendoar:2003-11-05T00:00Scientia Agrícola (Online) - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Identification of a non-host plant of Xylella fastidiosa to rear healthy sharpshooter vectors |
title |
Identification of a non-host plant of Xylella fastidiosa to rear healthy sharpshooter vectors |
spellingShingle |
Identification of a non-host plant of Xylella fastidiosa to rear healthy sharpshooter vectors Marucci,Rosangela Cristina Vernonia condensata citrus variegated chlorosis coffee leaf scorch sharpshooter vectors rearing technique |
title_short |
Identification of a non-host plant of Xylella fastidiosa to rear healthy sharpshooter vectors |
title_full |
Identification of a non-host plant of Xylella fastidiosa to rear healthy sharpshooter vectors |
title_fullStr |
Identification of a non-host plant of Xylella fastidiosa to rear healthy sharpshooter vectors |
title_full_unstemmed |
Identification of a non-host plant of Xylella fastidiosa to rear healthy sharpshooter vectors |
title_sort |
Identification of a non-host plant of Xylella fastidiosa to rear healthy sharpshooter vectors |
author |
Marucci,Rosangela Cristina |
author_facet |
Marucci,Rosangela Cristina Giustolin,Teresinha Augusta Miranda,Marcelo Pedreira de Miquelote,Helen Almeida,Rodrigo Piacentini Paes de Lopes,João Roberto Spotti |
author_role |
author |
author2 |
Giustolin,Teresinha Augusta Miranda,Marcelo Pedreira de Miquelote,Helen Almeida,Rodrigo Piacentini Paes de Lopes,João Roberto Spotti |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Marucci,Rosangela Cristina Giustolin,Teresinha Augusta Miranda,Marcelo Pedreira de Miquelote,Helen Almeida,Rodrigo Piacentini Paes de Lopes,João Roberto Spotti |
dc.subject.por.fl_str_mv |
Vernonia condensata citrus variegated chlorosis coffee leaf scorch sharpshooter vectors rearing technique |
topic |
Vernonia condensata citrus variegated chlorosis coffee leaf scorch sharpshooter vectors rearing technique |
description |
Rearing leafhopper (Hemiptera: Cicadellidae) vectors free of Xylella fastidiosa is a requirement for studies of various aspects of vector-pathogen interactions. The selection of a plant that allows vector development but not bacterial multiplication is desirable to produce healthy vectors. In this study, two leafhopper hosts, Vernonia condensata ('boldo') and Aloysia virgata ('lixeira') were needle inoculated with citrus and coffee strains of X. fastidiosa to evaluate if these plants support pathogen colonization. The inoculated plants did not present symptoms and the pathogen was not detected by culture and PCR tests, neither soon after inoculation (7-14 days) nor later, at 1, 4, 6 and 12 months after inoculation. To obtain healthy adults of the leafhopper vectors Acrogonia citrina, Bucephalogonia xanthophis, Dilobopterus costalimai, Homalodisca ignorata and Oncometopia facialis, early-instar nymphs were reared on V. condensata. X. fastidiosa was not detected in any of 175 adults obtained. V. condensata and A. virgata are nonpropagative hosts of X. fastidiosa and enable the production of healthy leafhoppers for vector studies. |
publishDate |
2003 |
dc.date.none.fl_str_mv |
2003-12-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162003000400010 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162003000400010 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0103-90162003000400010 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Escola Superior de Agricultura "Luiz de Queiroz" |
publisher.none.fl_str_mv |
Escola Superior de Agricultura "Luiz de Queiroz" |
dc.source.none.fl_str_mv |
Scientia Agricola v.60 n.4 2003 reponame:Scientia Agrícola (Online) instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Scientia Agrícola (Online) |
collection |
Scientia Agrícola (Online) |
repository.name.fl_str_mv |
Scientia Agrícola (Online) - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
scientia@usp.br||alleoni@usp.br |
_version_ |
1748936459182669824 |