Atividade da enzima acetilcolinesterase em cérebro e músculo de carpas após exposição aguda ao diafuran
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Scientia Agrícola (Online) |
Texto Completo: | https://www.revistas.usp.br/sa/article/view/22304 |
Resumo: | Sublethal adverse effects may result from exposure of aquatic organisms to insecticides at environmentally relevant concentrations. Fingerlings of the common carp (Cyprinus carpio, Linnaeus, 1758), grass carp (Ctenopharyngodon idella, Valenciennes, 1844), and bighead carp (Aristichthys nobilis, Richardson, 1845) were exposed to diafuran, an insecticide widely used during rice cultivation in Southern Brazil. The aim of this study was to verify the relationship between the lethal concentration (LC50) of diafuran and the acetylcholinesterase (AChE) activity in brain and muscle tissues of these species as a possible early biomarker of exposure to this insecticide. LC50 was determined for fish exposed to diafuran concentrations during 96 h (short term): common carp: control, 0.5, 1.0, 1.5, 2.0, 2.5 and 3.0 mg L-1; grass carp: control, 1.0, 2.0, 3.0 and 3.5 mg L-1 and, bighead carp: control, 0.5, 1.0, 1.5, 2.0, 3.0 and 4.0 mg L-1, as well as the determination of AChE at concentrations near LC50 for these species. LC50 values (nominal concentrations) were 1.81 mg L-1 for the common carp, 2.71 mg L-1 for the grass carp and, 2.37 mg L-1 for the bighead carp. All carps exposed to diafuran were lethargic (lower concentrations) or immobile. Diafuran inhibited the acetylcholinesterase activity in brain (~38%) and muscle (~50%) of all species. Muscle of bighead carp under control treatment showed higher specific AChE activity than brain (14.44 against 5.94 µmol min-1 g protein-1, respectively). Concentrations of diafuran used for rice cropping may affect Cyprinus carpio, Ctenopharyngodon idella and Aristichthys nobilis behaviors and the AChE activities in brain and muscle of these species may be an early biomarker of toxicity of this insecticide. |
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Atividade da enzima acetilcolinesterase em cérebro e músculo de carpas após exposição aguda ao diafuran Acetylcholinesterase enzyme activity in carp brain and muscle after acute exposure to diafuran Cyprinus carpioCtenopharyngodon idellaAristichthys nobilisagroquímicoconcentração letalCyprinus carpioCtenopharyngodon idellaAristichthys nobilispesticidelethal concentration Sublethal adverse effects may result from exposure of aquatic organisms to insecticides at environmentally relevant concentrations. Fingerlings of the common carp (Cyprinus carpio, Linnaeus, 1758), grass carp (Ctenopharyngodon idella, Valenciennes, 1844), and bighead carp (Aristichthys nobilis, Richardson, 1845) were exposed to diafuran, an insecticide widely used during rice cultivation in Southern Brazil. The aim of this study was to verify the relationship between the lethal concentration (LC50) of diafuran and the acetylcholinesterase (AChE) activity in brain and muscle tissues of these species as a possible early biomarker of exposure to this insecticide. LC50 was determined for fish exposed to diafuran concentrations during 96 h (short term): common carp: control, 0.5, 1.0, 1.5, 2.0, 2.5 and 3.0 mg L-1; grass carp: control, 1.0, 2.0, 3.0 and 3.5 mg L-1 and, bighead carp: control, 0.5, 1.0, 1.5, 2.0, 3.0 and 4.0 mg L-1, as well as the determination of AChE at concentrations near LC50 for these species. LC50 values (nominal concentrations) were 1.81 mg L-1 for the common carp, 2.71 mg L-1 for the grass carp and, 2.37 mg L-1 for the bighead carp. All carps exposed to diafuran were lethargic (lower concentrations) or immobile. Diafuran inhibited the acetylcholinesterase activity in brain (~38%) and muscle (~50%) of all species. Muscle of bighead carp under control treatment showed higher specific AChE activity than brain (14.44 against 5.94 µmol min-1 g protein-1, respectively). Concentrations of diafuran used for rice cropping may affect Cyprinus carpio, Ctenopharyngodon idella and Aristichthys nobilis behaviors and the AChE activities in brain and muscle of these species may be an early biomarker of toxicity of this insecticide. Exposição a inseticidas em concentrações elevadas no ambiente podem ocasionar efeitos adversos subletais em organismos aquáticos. Alevinos de carpa húngara (Cyprinus carpio, Linnaeus, 1758), carpa capim (Ctenopharyngodon idella, Valenciennes, 1844) e carpa cabeça grande (Aristichthys nobilis, Richardson, 1845) foram expostos ao diafuran, um inseticida utilizado na cultura do arroz no sul do Brasil. O objetivo deste estudo foi verificar a relação entre concentração letal mediana (CL50) do diafuran e a atividade da enzima acetilcolinesterase (AChE) em cérebro e músculo dessas espécies, como um possível biomarcador inicial da exposição a este inseticida. A CL50 foi determinada com peixes expostos a concentrações de diafuran em 96 h: carpa húngara: controle; 0,5; 1,0; 1,5; 2,0; 2,5 e 3,0 mg L-1; carpa capim: controle; 1,0; 2,0; 3,0 e 3,5 mg L-1 e carpa cabeça grande: controle; 0,5; 1,0; 1,5; 2,0; 3,0 e 4,0 mg L-1, bem como a determinação da AChE em concentrações próximas da CL50 para essas espécies. Valores de CL50 (concentrações nominais) foram de 1,81 mg L-1 para carpa húngara, 2,71 mg L-1 para carpa capim e 2,37 mg L-1 para carpa cabeça grande. Todas as carpas expostas ao diafuran estavam letárgicas (menores concentrações) ou imóveis. Diafuran inibiu significativamente a atividade da AChE em cérebro (~38 %) e músculo (~50 %) de todas as espécies estudadas. Atividade da AChE em músculo para carpa cabeça grande foi mais alta que cérebro (14,44 contra 5,94 µmol min-1 g proteína-1, respectivamente). Este estudo demonstrou que concentrações de diafuran utilizadas na cultura do arroz podem afetar o comportamento de Cyprinus carpio, Ctenopharyngodon idella e Aristichthys nobilis, e a atividade da acetilcolinesterase cerebral e muscular pode ser um biomarcador inicial de toxicidade deste inseticida. Universidade de São Paulo. Escola Superior de Agricultura Luiz de Queiroz2008-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/sa/article/view/2230410.1590/S0103-90162008000400003Scientia Agricola; v. 65 n. 4 (2008); 340-345Scientia Agricola; Vol. 65 No. 4 (2008); 340-345Scientia Agricola; Vol. 65 Núm. 4 (2008); 340-3451678-992X0103-9016reponame:Scientia Agrícola (Online)instname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/sa/article/view/22304/24328Copyright (c) 2015 Scientia Agricolainfo:eu-repo/semantics/openAccessGolombieski, Jaqueline IneuMarchesan, EnioCamargo, Edinalvo RabaioliSalbego, JoseâniaBaumart, Joele SchmittLoro, Vania LuciaMachado, Sérgio Luiz de OliveiraZanella, RenatoBaldisserotto, Bernardo2015-07-07T18:02:26Zoai:revistas.usp.br:article/22304Revistahttp://revistas.usp.br/sa/indexPUBhttps://old.scielo.br/oai/scielo-oai.phpscientia@usp.br||alleoni@usp.br1678-992X0103-9016opendoar:2015-07-07T18:02:26Scientia Agrícola (Online) - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Atividade da enzima acetilcolinesterase em cérebro e músculo de carpas após exposição aguda ao diafuran Acetylcholinesterase enzyme activity in carp brain and muscle after acute exposure to diafuran |
title |
Atividade da enzima acetilcolinesterase em cérebro e músculo de carpas após exposição aguda ao diafuran |
spellingShingle |
Atividade da enzima acetilcolinesterase em cérebro e músculo de carpas após exposição aguda ao diafuran Golombieski, Jaqueline Ineu Cyprinus carpio Ctenopharyngodon idella Aristichthys nobilis agroquímico concentração letal Cyprinus carpio Ctenopharyngodon idella Aristichthys nobilis pesticide lethal concentration |
title_short |
Atividade da enzima acetilcolinesterase em cérebro e músculo de carpas após exposição aguda ao diafuran |
title_full |
Atividade da enzima acetilcolinesterase em cérebro e músculo de carpas após exposição aguda ao diafuran |
title_fullStr |
Atividade da enzima acetilcolinesterase em cérebro e músculo de carpas após exposição aguda ao diafuran |
title_full_unstemmed |
Atividade da enzima acetilcolinesterase em cérebro e músculo de carpas após exposição aguda ao diafuran |
title_sort |
Atividade da enzima acetilcolinesterase em cérebro e músculo de carpas após exposição aguda ao diafuran |
author |
Golombieski, Jaqueline Ineu |
author_facet |
Golombieski, Jaqueline Ineu Marchesan, Enio Camargo, Edinalvo Rabaioli Salbego, Joseânia Baumart, Joele Schmitt Loro, Vania Lucia Machado, Sérgio Luiz de Oliveira Zanella, Renato Baldisserotto, Bernardo |
author_role |
author |
author2 |
Marchesan, Enio Camargo, Edinalvo Rabaioli Salbego, Joseânia Baumart, Joele Schmitt Loro, Vania Lucia Machado, Sérgio Luiz de Oliveira Zanella, Renato Baldisserotto, Bernardo |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Golombieski, Jaqueline Ineu Marchesan, Enio Camargo, Edinalvo Rabaioli Salbego, Joseânia Baumart, Joele Schmitt Loro, Vania Lucia Machado, Sérgio Luiz de Oliveira Zanella, Renato Baldisserotto, Bernardo |
dc.subject.por.fl_str_mv |
Cyprinus carpio Ctenopharyngodon idella Aristichthys nobilis agroquímico concentração letal Cyprinus carpio Ctenopharyngodon idella Aristichthys nobilis pesticide lethal concentration |
topic |
Cyprinus carpio Ctenopharyngodon idella Aristichthys nobilis agroquímico concentração letal Cyprinus carpio Ctenopharyngodon idella Aristichthys nobilis pesticide lethal concentration |
description |
Sublethal adverse effects may result from exposure of aquatic organisms to insecticides at environmentally relevant concentrations. Fingerlings of the common carp (Cyprinus carpio, Linnaeus, 1758), grass carp (Ctenopharyngodon idella, Valenciennes, 1844), and bighead carp (Aristichthys nobilis, Richardson, 1845) were exposed to diafuran, an insecticide widely used during rice cultivation in Southern Brazil. The aim of this study was to verify the relationship between the lethal concentration (LC50) of diafuran and the acetylcholinesterase (AChE) activity in brain and muscle tissues of these species as a possible early biomarker of exposure to this insecticide. LC50 was determined for fish exposed to diafuran concentrations during 96 h (short term): common carp: control, 0.5, 1.0, 1.5, 2.0, 2.5 and 3.0 mg L-1; grass carp: control, 1.0, 2.0, 3.0 and 3.5 mg L-1 and, bighead carp: control, 0.5, 1.0, 1.5, 2.0, 3.0 and 4.0 mg L-1, as well as the determination of AChE at concentrations near LC50 for these species. LC50 values (nominal concentrations) were 1.81 mg L-1 for the common carp, 2.71 mg L-1 for the grass carp and, 2.37 mg L-1 for the bighead carp. All carps exposed to diafuran were lethargic (lower concentrations) or immobile. Diafuran inhibited the acetylcholinesterase activity in brain (~38%) and muscle (~50%) of all species. Muscle of bighead carp under control treatment showed higher specific AChE activity than brain (14.44 against 5.94 µmol min-1 g protein-1, respectively). Concentrations of diafuran used for rice cropping may affect Cyprinus carpio, Ctenopharyngodon idella and Aristichthys nobilis behaviors and the AChE activities in brain and muscle of these species may be an early biomarker of toxicity of this insecticide. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/sa/article/view/22304 10.1590/S0103-90162008000400003 |
url |
https://www.revistas.usp.br/sa/article/view/22304 |
identifier_str_mv |
10.1590/S0103-90162008000400003 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/sa/article/view/22304/24328 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2015 Scientia Agricola info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2015 Scientia Agricola |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Escola Superior de Agricultura Luiz de Queiroz |
publisher.none.fl_str_mv |
Universidade de São Paulo. Escola Superior de Agricultura Luiz de Queiroz |
dc.source.none.fl_str_mv |
Scientia Agricola; v. 65 n. 4 (2008); 340-345 Scientia Agricola; Vol. 65 No. 4 (2008); 340-345 Scientia Agricola; Vol. 65 Núm. 4 (2008); 340-345 1678-992X 0103-9016 reponame:Scientia Agrícola (Online) instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Scientia Agrícola (Online) |
collection |
Scientia Agrícola (Online) |
repository.name.fl_str_mv |
Scientia Agrícola (Online) - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
scientia@usp.br||alleoni@usp.br |
_version_ |
1787713256741142528 |