A simple method for DNA isolation from Xanthomonas spp.

Detalhes bibliográficos
Autor(a) principal: Gomes,Luiz Humberto
Data de Publicação: 2000
Outros Autores: Duarte,Keila Maria Roncato, Andrino,Felipe Gabriel, Tavares,Flavio Cesar Almeida
Tipo de documento: Relatório
Idioma: eng
Título da fonte: Scientia Agrícola (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162000000300028
Resumo: A simple DNA isolation method was developed with routine chemicals that yields high quality and integrity preparations when compared to some of the most well known protocols. The method described does not require the use of lysing enzymes, water bath and the DNA was obtained within 40 minutes The amount of nucleic acid extracted (measured in terms of absorbancy at 260 nm) from strains of Xanthomonas spp., Pseudomonas spp. and Erwinia spp. was two to five times higher than that of the most commonly used method.
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spelling A simple method for DNA isolation from Xanthomonas spp.DNAXanthomonasRAPDA simple DNA isolation method was developed with routine chemicals that yields high quality and integrity preparations when compared to some of the most well known protocols. The method described does not require the use of lysing enzymes, water bath and the DNA was obtained within 40 minutes The amount of nucleic acid extracted (measured in terms of absorbancy at 260 nm) from strains of Xanthomonas spp., Pseudomonas spp. and Erwinia spp. was two to five times higher than that of the most commonly used method.Escola Superior de Agricultura "Luiz de Queiroz"2000-09-01info:eu-repo/semantics/reportinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162000000300028Scientia Agricola v.57 n.3 2000reponame:Scientia Agrícola (Online)instname:Universidade de São Paulo (USP)instacron:USP10.1590/S0103-90162000000300028info:eu-repo/semantics/openAccessGomes,Luiz HumbertoDuarte,Keila Maria RoncatoAndrino,Felipe GabrielTavares,Flavio Cesar Almeidaeng2000-09-21T00:00:00Zoai:scielo:S0103-90162000000300028Revistahttp://revistas.usp.br/sa/indexPUBhttps://old.scielo.br/oai/scielo-oai.phpscientia@usp.br||alleoni@usp.br1678-992X0103-9016opendoar:2000-09-21T00:00Scientia Agrícola (Online) - Universidade de São Paulo (USP)false
dc.title.none.fl_str_mv A simple method for DNA isolation from Xanthomonas spp.
title A simple method for DNA isolation from Xanthomonas spp.
spellingShingle A simple method for DNA isolation from Xanthomonas spp.
Gomes,Luiz Humberto
DNA
Xanthomonas
RAPD
title_short A simple method for DNA isolation from Xanthomonas spp.
title_full A simple method for DNA isolation from Xanthomonas spp.
title_fullStr A simple method for DNA isolation from Xanthomonas spp.
title_full_unstemmed A simple method for DNA isolation from Xanthomonas spp.
title_sort A simple method for DNA isolation from Xanthomonas spp.
author Gomes,Luiz Humberto
author_facet Gomes,Luiz Humberto
Duarte,Keila Maria Roncato
Andrino,Felipe Gabriel
Tavares,Flavio Cesar Almeida
author_role author
author2 Duarte,Keila Maria Roncato
Andrino,Felipe Gabriel
Tavares,Flavio Cesar Almeida
author2_role author
author
author
dc.contributor.author.fl_str_mv Gomes,Luiz Humberto
Duarte,Keila Maria Roncato
Andrino,Felipe Gabriel
Tavares,Flavio Cesar Almeida
dc.subject.por.fl_str_mv DNA
Xanthomonas
RAPD
topic DNA
Xanthomonas
RAPD
description A simple DNA isolation method was developed with routine chemicals that yields high quality and integrity preparations when compared to some of the most well known protocols. The method described does not require the use of lysing enzymes, water bath and the DNA was obtained within 40 minutes The amount of nucleic acid extracted (measured in terms of absorbancy at 260 nm) from strains of Xanthomonas spp., Pseudomonas spp. and Erwinia spp. was two to five times higher than that of the most commonly used method.
publishDate 2000
dc.date.none.fl_str_mv 2000-09-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/report
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format report
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162000000300028
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162000000300028
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0103-90162000000300028
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Escola Superior de Agricultura "Luiz de Queiroz"
publisher.none.fl_str_mv Escola Superior de Agricultura "Luiz de Queiroz"
dc.source.none.fl_str_mv Scientia Agricola v.57 n.3 2000
reponame:Scientia Agrícola (Online)
instname:Universidade de São Paulo (USP)
instacron:USP
instname_str Universidade de São Paulo (USP)
instacron_str USP
institution USP
reponame_str Scientia Agrícola (Online)
collection Scientia Agrícola (Online)
repository.name.fl_str_mv Scientia Agrícola (Online) - Universidade de São Paulo (USP)
repository.mail.fl_str_mv scientia@usp.br||alleoni@usp.br
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