Isolation and growth of citrus rootstock protoplants
Autor(a) principal: | |
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Data de Publicação: | 1995 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Scientia Agrícola (Online) |
Texto Completo: | https://www.revistas.usp.br/sa/article/view/20286 |
Resumo: | Protoplast isolation and growth of Citrus rootstock v. Cravo lime and Cleópatra mandarin were carried out Enzyme mixture was used to digest cell suspensions 7-10 times, following criterious purification. Protoplast culture was carried out in doplets of semi-solid (agarose 0.6% p/v) KM8p medium, in the dark, at 28 ± 1°C following density adjustment to 2 x 10(5) ppts/ml. Osmotic pressure reduction was acomplished for each growth every 10 days with a mixture in equal volumes of KM8p and KM8 medium in ratios of 2:1; 1:1; 1:2 e 0:3. Isolation produced 0.7 x 10(6) ppts/ml for Cleópatra mandarin and 0.5 x 10(6) ppts/ml for Cravo lime, with a viability between 86 to 92% and 73 to 80%, respectively. First cell divisions were recorded at the 8th. day of growth, with an inicial platting efficiency of 9% for Cleópatra mandarin and 5% for Cravo lime. Callus development was achived in 50 days upon transfer of microcalus to semi-solid MT medium. |
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Isolation and growth of citrus rootstock protoplants Isolamento e cultivo de protoplastos de porta-enxertos de citros porta-enxertocitrosprotoplastosrootstockcitrusprotoplasts Protoplast isolation and growth of Citrus rootstock v. Cravo lime and Cleópatra mandarin were carried out Enzyme mixture was used to digest cell suspensions 7-10 times, following criterious purification. Protoplast culture was carried out in doplets of semi-solid (agarose 0.6% p/v) KM8p medium, in the dark, at 28 ± 1°C following density adjustment to 2 x 10(5) ppts/ml. Osmotic pressure reduction was acomplished for each growth every 10 days with a mixture in equal volumes of KM8p and KM8 medium in ratios of 2:1; 1:1; 1:2 e 0:3. Isolation produced 0.7 x 10(6) ppts/ml for Cleópatra mandarin and 0.5 x 10(6) ppts/ml for Cravo lime, with a viability between 86 to 92% and 73 to 80%, respectively. First cell divisions were recorded at the 8th. day of growth, with an inicial platting efficiency of 9% for Cleópatra mandarin and 5% for Cravo lime. Callus development was achived in 50 days upon transfer of microcalus to semi-solid MT medium. Realizou-se o isolamento e cultivo de protolastos de porta-enxertos de citros das variedades limoeiro Cravo e tangerina Cleópatra. Utilizou-se solução enzimática para a digestão da parede celular de células em suspensão (7 a 10 subcultivos em meio de cultura MT) e procedeu-se a purificação criteriosa. O plaqueamento dos protoplastos foi realizado em meio de cultivo KM8p e na molaridade de 0.6 M, sob condições de escuro a 28 ± 1°C, após ajuste para a densidade de 2 x 10s ppts/mL A redução da pressão osmótica do meio de cultivo foi realizada a cada 10 dias na seguinte proporção de meio de cultura KM8p 0.6 M e KM8 (2:1, 1:1, 1:2, 0:3). Com relação aos resultados, obteve-se em média o isolamento de 0.7 x 10(6) ppts / ml de suspensões celulares de tangerina Cleópatra e 0.5 x 1O6 ppts / ml de suspensões de limoeiro Cravo, com uma porcentagem de viabilidade de 86 a 92% para a variedade Cleópatra e de 73 a 80% para o limoeiro Cravo. As divisões celulares começaram a ocorrer por volta do 8° dia após o plaqueamento, sendo obtida uma eficiência inicial de plaqueamento de 9% para a variedade Cleópatra e de 5% para o limoeiro Cravo. Aos 50 dias de cultivo, as colônias foram transferidas para meio semi-sólido MT, onde apresentaram ritmo acelerado de crescimento formando calos. Universidade de São Paulo. Escola Superior de Agricultura Luiz de Queiroz1995-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/sa/article/view/2028610.1590/S0103-90161995000200007Scientia Agricola; v. 52 n. 2 (1995); 244-248Scientia Agricola; Vol. 52 No. 2 (1995); 244-248Scientia Agricola; Vol. 52 Núm. 2 (1995); 244-2481678-992X0103-9016reponame:Scientia Agrícola (Online)instname:Universidade de São Paulo (USP)instacron:USPporhttps://www.revistas.usp.br/sa/article/view/20286/22404Copyright (c) 2015 Scientia Agricolainfo:eu-repo/semantics/openAccessOliveira, R.P. deMendes, B.M.J.d' Utra Vaz, F.B.Tulmann Neto, A.2015-07-07T11:27:08Zoai:revistas.usp.br:article/20286Revistahttp://revistas.usp.br/sa/indexPUBhttps://old.scielo.br/oai/scielo-oai.phpscientia@usp.br||alleoni@usp.br1678-992X0103-9016opendoar:2015-07-07T11:27:08Scientia Agrícola (Online) - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Isolation and growth of citrus rootstock protoplants Isolamento e cultivo de protoplastos de porta-enxertos de citros |
title |
Isolation and growth of citrus rootstock protoplants |
spellingShingle |
Isolation and growth of citrus rootstock protoplants Oliveira, R.P. de porta-enxerto citros protoplastos rootstock citrus protoplasts |
title_short |
Isolation and growth of citrus rootstock protoplants |
title_full |
Isolation and growth of citrus rootstock protoplants |
title_fullStr |
Isolation and growth of citrus rootstock protoplants |
title_full_unstemmed |
Isolation and growth of citrus rootstock protoplants |
title_sort |
Isolation and growth of citrus rootstock protoplants |
author |
Oliveira, R.P. de |
author_facet |
Oliveira, R.P. de Mendes, B.M.J. d' Utra Vaz, F.B. Tulmann Neto, A. |
author_role |
author |
author2 |
Mendes, B.M.J. d' Utra Vaz, F.B. Tulmann Neto, A. |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Oliveira, R.P. de Mendes, B.M.J. d' Utra Vaz, F.B. Tulmann Neto, A. |
dc.subject.por.fl_str_mv |
porta-enxerto citros protoplastos rootstock citrus protoplasts |
topic |
porta-enxerto citros protoplastos rootstock citrus protoplasts |
description |
Protoplast isolation and growth of Citrus rootstock v. Cravo lime and Cleópatra mandarin were carried out Enzyme mixture was used to digest cell suspensions 7-10 times, following criterious purification. Protoplast culture was carried out in doplets of semi-solid (agarose 0.6% p/v) KM8p medium, in the dark, at 28 ± 1°C following density adjustment to 2 x 10(5) ppts/ml. Osmotic pressure reduction was acomplished for each growth every 10 days with a mixture in equal volumes of KM8p and KM8 medium in ratios of 2:1; 1:1; 1:2 e 0:3. Isolation produced 0.7 x 10(6) ppts/ml for Cleópatra mandarin and 0.5 x 10(6) ppts/ml for Cravo lime, with a viability between 86 to 92% and 73 to 80%, respectively. First cell divisions were recorded at the 8th. day of growth, with an inicial platting efficiency of 9% for Cleópatra mandarin and 5% for Cravo lime. Callus development was achived in 50 days upon transfer of microcalus to semi-solid MT medium. |
publishDate |
1995 |
dc.date.none.fl_str_mv |
1995-08-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/sa/article/view/20286 10.1590/S0103-90161995000200007 |
url |
https://www.revistas.usp.br/sa/article/view/20286 |
identifier_str_mv |
10.1590/S0103-90161995000200007 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/sa/article/view/20286/22404 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2015 Scientia Agricola info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2015 Scientia Agricola |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Escola Superior de Agricultura Luiz de Queiroz |
publisher.none.fl_str_mv |
Universidade de São Paulo. Escola Superior de Agricultura Luiz de Queiroz |
dc.source.none.fl_str_mv |
Scientia Agricola; v. 52 n. 2 (1995); 244-248 Scientia Agricola; Vol. 52 No. 2 (1995); 244-248 Scientia Agricola; Vol. 52 Núm. 2 (1995); 244-248 1678-992X 0103-9016 reponame:Scientia Agrícola (Online) instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Scientia Agrícola (Online) |
collection |
Scientia Agrícola (Online) |
repository.name.fl_str_mv |
Scientia Agrícola (Online) - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
scientia@usp.br||alleoni@usp.br |
_version_ |
1787713252025696256 |