Reduced sensitivity to azoxystrobin is stable in Monilinia fructicola isolates
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Scientia Agrícola (Online) |
Texto Completo: | https://www.revistas.usp.br/sa/article/view/130938 |
Resumo: | A shift towards declining azoxystrobin sensitivity has been previously documented in a collection of Brazilian Monilinia fructicola isolates, but information on the stability of this trait after in vitro sub-culturing is not available. In this study, one highly-sensitive isolate and two isolates with reduced sensitivity to azoxystrobin, not exhibiting point mutations at codons 129, 137 and 143 in the target gene of cytochrome b were used. Two independent experiments consisted of 10 weekly transfers of each strain in potato dextrose agar (PDA). Conidial production and germination were quantified in the initial culture and, again, after the third, sixth and tenth transfer. Measures of mycelial growth were obtained in colonies growing on PDA amended with azoxystrobin at 1 µg mL−1 and salicylhydroxamic acid at 100 µg mL−1. Data were normalized to mycelial growth rate after each of the 10 transfers. The colony diameter in the less sensitive isolates was stable across the transfers in the PDA amended with the fungicide. The conidial production and mycelial growth rate did not decrease in non-amended media either. Our results suggest that the trait of reduced sensitivity to azoxystrobin is stable under no selection pressure for azoxystrobin. |
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Reduced sensitivity to azoxystrobin is stable in Monilinia fructicola isolatesPrunusQuinone-outside inhibitorA shift towards declining azoxystrobin sensitivity has been previously documented in a collection of Brazilian Monilinia fructicola isolates, but information on the stability of this trait after in vitro sub-culturing is not available. In this study, one highly-sensitive isolate and two isolates with reduced sensitivity to azoxystrobin, not exhibiting point mutations at codons 129, 137 and 143 in the target gene of cytochrome b were used. Two independent experiments consisted of 10 weekly transfers of each strain in potato dextrose agar (PDA). Conidial production and germination were quantified in the initial culture and, again, after the third, sixth and tenth transfer. Measures of mycelial growth were obtained in colonies growing on PDA amended with azoxystrobin at 1 µg mL−1 and salicylhydroxamic acid at 100 µg mL−1. Data were normalized to mycelial growth rate after each of the 10 transfers. The colony diameter in the less sensitive isolates was stable across the transfers in the PDA amended with the fungicide. The conidial production and mycelial growth rate did not decrease in non-amended media either. Our results suggest that the trait of reduced sensitivity to azoxystrobin is stable under no selection pressure for azoxystrobin.Universidade de São Paulo. Escola Superior de Agricultura Luiz de Queiroz2017-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/sa/article/view/13093810.1590/1678-992x-2015-0469Scientia Agricola; v. 74 n. 2 (2017); 169-173Scientia Agricola; Vol. 74 Núm. 2 (2017); 169-173Scientia Agricola; Vol. 74 No. 2 (2017); 169-1731678-992X0103-9016reponame:Scientia Agrícola (Online)instname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/sa/article/view/130938/127397Copyright (c) 2017 Scientia Agricolainfo:eu-repo/semantics/openAccessPrimiano, Isabela VescoveMolina, Juan Pablo EdwardsMio, Louise Larissa May DePeres, Natalia AparecidaAmorim, Lilian2017-06-12T11:50:07Zoai:revistas.usp.br:article/130938Revistahttp://revistas.usp.br/sa/indexPUBhttps://old.scielo.br/oai/scielo-oai.phpscientia@usp.br||alleoni@usp.br1678-992X0103-9016opendoar:2017-06-12T11:50:07Scientia Agrícola (Online) - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Reduced sensitivity to azoxystrobin is stable in Monilinia fructicola isolates |
title |
Reduced sensitivity to azoxystrobin is stable in Monilinia fructicola isolates |
spellingShingle |
Reduced sensitivity to azoxystrobin is stable in Monilinia fructicola isolates Primiano, Isabela Vescove Prunus Quinone-outside inhibitor |
title_short |
Reduced sensitivity to azoxystrobin is stable in Monilinia fructicola isolates |
title_full |
Reduced sensitivity to azoxystrobin is stable in Monilinia fructicola isolates |
title_fullStr |
Reduced sensitivity to azoxystrobin is stable in Monilinia fructicola isolates |
title_full_unstemmed |
Reduced sensitivity to azoxystrobin is stable in Monilinia fructicola isolates |
title_sort |
Reduced sensitivity to azoxystrobin is stable in Monilinia fructicola isolates |
author |
Primiano, Isabela Vescove |
author_facet |
Primiano, Isabela Vescove Molina, Juan Pablo Edwards Mio, Louise Larissa May De Peres, Natalia Aparecida Amorim, Lilian |
author_role |
author |
author2 |
Molina, Juan Pablo Edwards Mio, Louise Larissa May De Peres, Natalia Aparecida Amorim, Lilian |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Primiano, Isabela Vescove Molina, Juan Pablo Edwards Mio, Louise Larissa May De Peres, Natalia Aparecida Amorim, Lilian |
dc.subject.por.fl_str_mv |
Prunus Quinone-outside inhibitor |
topic |
Prunus Quinone-outside inhibitor |
description |
A shift towards declining azoxystrobin sensitivity has been previously documented in a collection of Brazilian Monilinia fructicola isolates, but information on the stability of this trait after in vitro sub-culturing is not available. In this study, one highly-sensitive isolate and two isolates with reduced sensitivity to azoxystrobin, not exhibiting point mutations at codons 129, 137 and 143 in the target gene of cytochrome b were used. Two independent experiments consisted of 10 weekly transfers of each strain in potato dextrose agar (PDA). Conidial production and germination were quantified in the initial culture and, again, after the third, sixth and tenth transfer. Measures of mycelial growth were obtained in colonies growing on PDA amended with azoxystrobin at 1 µg mL−1 and salicylhydroxamic acid at 100 µg mL−1. Data were normalized to mycelial growth rate after each of the 10 transfers. The colony diameter in the less sensitive isolates was stable across the transfers in the PDA amended with the fungicide. The conidial production and mycelial growth rate did not decrease in non-amended media either. Our results suggest that the trait of reduced sensitivity to azoxystrobin is stable under no selection pressure for azoxystrobin. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-04-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/sa/article/view/130938 10.1590/1678-992x-2015-0469 |
url |
https://www.revistas.usp.br/sa/article/view/130938 |
identifier_str_mv |
10.1590/1678-992x-2015-0469 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/sa/article/view/130938/127397 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2017 Scientia Agricola info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2017 Scientia Agricola |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Escola Superior de Agricultura Luiz de Queiroz |
publisher.none.fl_str_mv |
Universidade de São Paulo. Escola Superior de Agricultura Luiz de Queiroz |
dc.source.none.fl_str_mv |
Scientia Agricola; v. 74 n. 2 (2017); 169-173 Scientia Agricola; Vol. 74 Núm. 2 (2017); 169-173 Scientia Agricola; Vol. 74 No. 2 (2017); 169-173 1678-992X 0103-9016 reponame:Scientia Agrícola (Online) instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Scientia Agrícola (Online) |
collection |
Scientia Agrícola (Online) |
repository.name.fl_str_mv |
Scientia Agrícola (Online) - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
scientia@usp.br||alleoni@usp.br |
_version_ |
1800222792944189440 |