Association mapping in common bean revealed regions associated with Anthracnose and Angular Leaf Spot resistance
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Scientia Agrícola (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162019001400321 |
Resumo: | ABSTRACT: Despite important biotic stresses to common bean, Anthracnose (ANT) and Angular Leaf Spot (ALS) can cause losses of up to 80 % and occur in more than 60 countries around the world. Genetic resistance is the most sustainable strategy to manage these diseases. Thus, we aimed to (1) identify new SNP markers associated with ALS and ANT resistance loci in elite common bean lines, and (2) provide a functional characterization of the DNA sequences containing the identified SNP markers. We evaluated 60 inbred lines, under field conditions, which represent the elite germplasm developed by the Embrapa common bean breeding program across 22 years, in terms of severity of the ALS and ANT. The lines were genotyped with 5,398 SNPs. Then, a Mixed Linear Model was run to determine the SNP-trait associations. We observed two-significant marker-trait associations reacting to ANT, both located on chromosome Pv-02. These markers explained 25 % of the phenotypic variation. For ALS, only one significant marker-trait association was observed, which is located in chromosome Pv-10 and explained 19 % of the phenotypic variation. These markers, along with others already used, will be useful to add or keep ANT and ALS resistance loci identified in this work in the new carioca and black seeded cultivars. |
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Scientia Agrícola (Online) |
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Association mapping in common bean revealed regions associated with Anthracnose and Angular Leaf Spot resistanceColletotrichum lindemuthianumPseudocercospora griseoladisease resistanceelite linesmarker-assisted selectionABSTRACT: Despite important biotic stresses to common bean, Anthracnose (ANT) and Angular Leaf Spot (ALS) can cause losses of up to 80 % and occur in more than 60 countries around the world. Genetic resistance is the most sustainable strategy to manage these diseases. Thus, we aimed to (1) identify new SNP markers associated with ALS and ANT resistance loci in elite common bean lines, and (2) provide a functional characterization of the DNA sequences containing the identified SNP markers. We evaluated 60 inbred lines, under field conditions, which represent the elite germplasm developed by the Embrapa common bean breeding program across 22 years, in terms of severity of the ALS and ANT. The lines were genotyped with 5,398 SNPs. Then, a Mixed Linear Model was run to determine the SNP-trait associations. We observed two-significant marker-trait associations reacting to ANT, both located on chromosome Pv-02. These markers explained 25 % of the phenotypic variation. For ALS, only one significant marker-trait association was observed, which is located in chromosome Pv-10 and explained 19 % of the phenotypic variation. These markers, along with others already used, will be useful to add or keep ANT and ALS resistance loci identified in this work in the new carioca and black seeded cultivars.Escola Superior de Agricultura "Luiz de Queiroz"2019-07-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162019001400321Scientia Agricola v.76 n.4 2019reponame:Scientia Agrícola (Online)instname:Universidade de São Paulo (USP)instacron:USP10.1590/1678-992x-2017-0306info:eu-repo/semantics/openAccessFritsche-Neto,RobertoSouza,Thiago Lívio Pessoa Oliveira dePereira,Helton SantosFaria,Luís Cláudio deMelo,Leonardo CunhaNovaes,EvandroBrum,Itaraju Junior BaracuhyJannink,Jean-Luceng2019-03-18T00:00:00Zoai:scielo:S0103-90162019001400321Revistahttp://revistas.usp.br/sa/indexPUBhttps://old.scielo.br/oai/scielo-oai.phpscientia@usp.br||alleoni@usp.br1678-992X0103-9016opendoar:2019-03-18T00:00Scientia Agrícola (Online) - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Association mapping in common bean revealed regions associated with Anthracnose and Angular Leaf Spot resistance |
title |
Association mapping in common bean revealed regions associated with Anthracnose and Angular Leaf Spot resistance |
spellingShingle |
Association mapping in common bean revealed regions associated with Anthracnose and Angular Leaf Spot resistance Fritsche-Neto,Roberto Colletotrichum lindemuthianum Pseudocercospora griseola disease resistance elite lines marker-assisted selection |
title_short |
Association mapping in common bean revealed regions associated with Anthracnose and Angular Leaf Spot resistance |
title_full |
Association mapping in common bean revealed regions associated with Anthracnose and Angular Leaf Spot resistance |
title_fullStr |
Association mapping in common bean revealed regions associated with Anthracnose and Angular Leaf Spot resistance |
title_full_unstemmed |
Association mapping in common bean revealed regions associated with Anthracnose and Angular Leaf Spot resistance |
title_sort |
Association mapping in common bean revealed regions associated with Anthracnose and Angular Leaf Spot resistance |
author |
Fritsche-Neto,Roberto |
author_facet |
Fritsche-Neto,Roberto Souza,Thiago Lívio Pessoa Oliveira de Pereira,Helton Santos Faria,Luís Cláudio de Melo,Leonardo Cunha Novaes,Evandro Brum,Itaraju Junior Baracuhy Jannink,Jean-Luc |
author_role |
author |
author2 |
Souza,Thiago Lívio Pessoa Oliveira de Pereira,Helton Santos Faria,Luís Cláudio de Melo,Leonardo Cunha Novaes,Evandro Brum,Itaraju Junior Baracuhy Jannink,Jean-Luc |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Fritsche-Neto,Roberto Souza,Thiago Lívio Pessoa Oliveira de Pereira,Helton Santos Faria,Luís Cláudio de Melo,Leonardo Cunha Novaes,Evandro Brum,Itaraju Junior Baracuhy Jannink,Jean-Luc |
dc.subject.por.fl_str_mv |
Colletotrichum lindemuthianum Pseudocercospora griseola disease resistance elite lines marker-assisted selection |
topic |
Colletotrichum lindemuthianum Pseudocercospora griseola disease resistance elite lines marker-assisted selection |
description |
ABSTRACT: Despite important biotic stresses to common bean, Anthracnose (ANT) and Angular Leaf Spot (ALS) can cause losses of up to 80 % and occur in more than 60 countries around the world. Genetic resistance is the most sustainable strategy to manage these diseases. Thus, we aimed to (1) identify new SNP markers associated with ALS and ANT resistance loci in elite common bean lines, and (2) provide a functional characterization of the DNA sequences containing the identified SNP markers. We evaluated 60 inbred lines, under field conditions, which represent the elite germplasm developed by the Embrapa common bean breeding program across 22 years, in terms of severity of the ALS and ANT. The lines were genotyped with 5,398 SNPs. Then, a Mixed Linear Model was run to determine the SNP-trait associations. We observed two-significant marker-trait associations reacting to ANT, both located on chromosome Pv-02. These markers explained 25 % of the phenotypic variation. For ALS, only one significant marker-trait association was observed, which is located in chromosome Pv-10 and explained 19 % of the phenotypic variation. These markers, along with others already used, will be useful to add or keep ANT and ALS resistance loci identified in this work in the new carioca and black seeded cultivars. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-07-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162019001400321 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162019001400321 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/1678-992x-2017-0306 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Escola Superior de Agricultura "Luiz de Queiroz" |
publisher.none.fl_str_mv |
Escola Superior de Agricultura "Luiz de Queiroz" |
dc.source.none.fl_str_mv |
Scientia Agricola v.76 n.4 2019 reponame:Scientia Agrícola (Online) instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Scientia Agrícola (Online) |
collection |
Scientia Agrícola (Online) |
repository.name.fl_str_mv |
Scientia Agrícola (Online) - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
scientia@usp.br||alleoni@usp.br |
_version_ |
1748936465127047168 |