Passive stiffness of rat skeletal muscle undernourished during fetal development

Detalhes bibliográficos
Autor(a) principal: Toscano, Ana Elisa
Data de Publicação: 2010
Outros Autores: Ferraz, Karla Monica, Castro, Raul Manhães de, Canon, Francis
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Clinics
Texto Completo: https://www.revistas.usp.br/clinics/article/view/18472
Resumo: OBJECTIVES: The aim of the study was to investigate the effect of fetal undernutrition on the passive mechanical properties of skeletal muscle of weaned and young adult rats. INTRODUCTION: A poor nutrition supply during fetal development affects physiological functions of the fetus. From a mechanical point of view, skeletal muscle can be also characterized by its resistance to passive stretch. METHODS: Male Wistar rats were divided into two groups according to their mother's diet during pregnancy: a control group (mothers fed a 17% protein diet) and an isocaloric low-protein group (mothers fed a 7.8% protein diet). At birth, all mothers received a standardized meal ad libitum. At the age of 25 and 90 days, the soleus muscle and extensor digitorum longus (EDL) muscles were removed in order to test the passive mechanical properties. A first mechanical test consisted of an incremental stepwise extension test using fast velocity stretching (500 mm/s) enabling us to measure, for each extension stepwise, the dynamic stress (σd) and the steady stress (σs). A second test consisted of a slow velocity stretch in order to calculate normalized stiffness and tangent modulus from the stress-strain relationship. RESULTS: The results for the mechanical properties showed an important increase in passive stiffness in both the soleus and EDL muscles in weaned rat. In contrast, no modification was observed in young adult rats. CONCLUSIONS: The increase in passive stiffness in skeletal muscle of weaned rat submitted to intrauterine undernutrition it is most likely due to changes in muscle passive stiffness.
id USP-19_2215a63b28314c9309a610afb327b6c7
oai_identifier_str oai:revistas.usp.br:article/18472
network_acronym_str USP-19
network_name_str Clinics
repository_id_str
spelling Passive stiffness of rat skeletal muscle undernourished during fetal development Passive stiffnessSkeletal muscleFetalDevelopmentUndernutrition OBJECTIVES: The aim of the study was to investigate the effect of fetal undernutrition on the passive mechanical properties of skeletal muscle of weaned and young adult rats. INTRODUCTION: A poor nutrition supply during fetal development affects physiological functions of the fetus. From a mechanical point of view, skeletal muscle can be also characterized by its resistance to passive stretch. METHODS: Male Wistar rats were divided into two groups according to their mother's diet during pregnancy: a control group (mothers fed a 17% protein diet) and an isocaloric low-protein group (mothers fed a 7.8% protein diet). At birth, all mothers received a standardized meal ad libitum. At the age of 25 and 90 days, the soleus muscle and extensor digitorum longus (EDL) muscles were removed in order to test the passive mechanical properties. A first mechanical test consisted of an incremental stepwise extension test using fast velocity stretching (500 mm/s) enabling us to measure, for each extension stepwise, the dynamic stress (σd) and the steady stress (σs). A second test consisted of a slow velocity stretch in order to calculate normalized stiffness and tangent modulus from the stress-strain relationship. RESULTS: The results for the mechanical properties showed an important increase in passive stiffness in both the soleus and EDL muscles in weaned rat. In contrast, no modification was observed in young adult rats. CONCLUSIONS: The increase in passive stiffness in skeletal muscle of weaned rat submitted to intrauterine undernutrition it is most likely due to changes in muscle passive stiffness. Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo2010-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/clinics/article/view/1847210.1590/S1807-59322010001200022Clinics; Vol. 65 No. 12 (2010); 1363-1369 Clinics; v. 65 n. 12 (2010); 1363-1369 Clinics; Vol. 65 Núm. 12 (2010); 1363-1369 1980-53221807-5932reponame:Clinicsinstname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/clinics/article/view/18472/20535Toscano, Ana ElisaFerraz, Karla MonicaCastro, Raul Manhães deCanon, Francisinfo:eu-repo/semantics/openAccess2012-05-23T11:26:12Zoai:revistas.usp.br:article/18472Revistahttps://www.revistas.usp.br/clinicsPUBhttps://www.revistas.usp.br/clinics/oai||clinics@hc.fm.usp.br1980-53221807-5932opendoar:2012-05-23T11:26:12Clinics - Universidade de São Paulo (USP)false
dc.title.none.fl_str_mv Passive stiffness of rat skeletal muscle undernourished during fetal development
title Passive stiffness of rat skeletal muscle undernourished during fetal development
spellingShingle Passive stiffness of rat skeletal muscle undernourished during fetal development
Toscano, Ana Elisa
Passive stiffness
Skeletal muscle
Fetal
Development
Undernutrition
title_short Passive stiffness of rat skeletal muscle undernourished during fetal development
title_full Passive stiffness of rat skeletal muscle undernourished during fetal development
title_fullStr Passive stiffness of rat skeletal muscle undernourished during fetal development
title_full_unstemmed Passive stiffness of rat skeletal muscle undernourished during fetal development
title_sort Passive stiffness of rat skeletal muscle undernourished during fetal development
author Toscano, Ana Elisa
author_facet Toscano, Ana Elisa
Ferraz, Karla Monica
Castro, Raul Manhães de
Canon, Francis
author_role author
author2 Ferraz, Karla Monica
Castro, Raul Manhães de
Canon, Francis
author2_role author
author
author
dc.contributor.author.fl_str_mv Toscano, Ana Elisa
Ferraz, Karla Monica
Castro, Raul Manhães de
Canon, Francis
dc.subject.por.fl_str_mv Passive stiffness
Skeletal muscle
Fetal
Development
Undernutrition
topic Passive stiffness
Skeletal muscle
Fetal
Development
Undernutrition
description OBJECTIVES: The aim of the study was to investigate the effect of fetal undernutrition on the passive mechanical properties of skeletal muscle of weaned and young adult rats. INTRODUCTION: A poor nutrition supply during fetal development affects physiological functions of the fetus. From a mechanical point of view, skeletal muscle can be also characterized by its resistance to passive stretch. METHODS: Male Wistar rats were divided into two groups according to their mother's diet during pregnancy: a control group (mothers fed a 17% protein diet) and an isocaloric low-protein group (mothers fed a 7.8% protein diet). At birth, all mothers received a standardized meal ad libitum. At the age of 25 and 90 days, the soleus muscle and extensor digitorum longus (EDL) muscles were removed in order to test the passive mechanical properties. A first mechanical test consisted of an incremental stepwise extension test using fast velocity stretching (500 mm/s) enabling us to measure, for each extension stepwise, the dynamic stress (σd) and the steady stress (σs). A second test consisted of a slow velocity stretch in order to calculate normalized stiffness and tangent modulus from the stress-strain relationship. RESULTS: The results for the mechanical properties showed an important increase in passive stiffness in both the soleus and EDL muscles in weaned rat. In contrast, no modification was observed in young adult rats. CONCLUSIONS: The increase in passive stiffness in skeletal muscle of weaned rat submitted to intrauterine undernutrition it is most likely due to changes in muscle passive stiffness.
publishDate 2010
dc.date.none.fl_str_mv 2010-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/clinics/article/view/18472
10.1590/S1807-59322010001200022
url https://www.revistas.usp.br/clinics/article/view/18472
identifier_str_mv 10.1590/S1807-59322010001200022
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/clinics/article/view/18472/20535
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo
publisher.none.fl_str_mv Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo
dc.source.none.fl_str_mv Clinics; Vol. 65 No. 12 (2010); 1363-1369
Clinics; v. 65 n. 12 (2010); 1363-1369
Clinics; Vol. 65 Núm. 12 (2010); 1363-1369
1980-5322
1807-5932
reponame:Clinics
instname:Universidade de São Paulo (USP)
instacron:USP
instname_str Universidade de São Paulo (USP)
instacron_str USP
institution USP
reponame_str Clinics
collection Clinics
repository.name.fl_str_mv Clinics - Universidade de São Paulo (USP)
repository.mail.fl_str_mv ||clinics@hc.fm.usp.br
_version_ 1800222755641098240