In vitro sensitivity of dermatophytes to urea

Detalhes bibliográficos
Autor(a) principal: Martins, José Eduardo Costa
Data de Publicação: 2006
Outros Autores: Corim, Simone Miwa, Arriagada, Giovanna Letícia Hernández, Melo, Natalina Takahashi de, Heins, Elisabeth Maria
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Clinics
Texto Completo: https://www.revistas.usp.br/clinics/article/view/17500
Resumo: OBJECTIVE: Urea is commonly used as a keratolytic substance in the treatment of onychomycoses to improve the penetration of antifungal drugs in the lesion sites. The aim of the present study was to investigate the inhibitory action of urea on samples of dermatophytes in vitro. METHOD: Minimum inhibitory concentration of urea was determined for 31 samples of dermatophytes cultured in Sabouraud-dextrose broth containing different concentrations (7.5% up to 40%) of urea. Absence of growth was the criterion adopted to determine the minimum inhibitory concentration. RESULTS: The majority of samples (87%) were sensitive to urea at 12.5%, or less. 2 isolates of Trichophyton tonsurans and 2 of Trichophyton rubrum required 30%, and 40% urea, respectively, to be completely inhibited. CONCLUSION: In vitro results demonstrate inhibitory activity of urea on dermatophytes, suggesting that it could be used as an adjuvant in topical treatments.
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spelling In vitro sensitivity of dermatophytes to urea Sensibilidade in vitro de dermatófitos à uréia OnicomicosesDermatomicosesDermatófitosUréiaAção InibitóriaOnychomycosesDermatophytosesDermatophytesUreaInhibitory Activity OBJECTIVE: Urea is commonly used as a keratolytic substance in the treatment of onychomycoses to improve the penetration of antifungal drugs in the lesion sites. The aim of the present study was to investigate the inhibitory action of urea on samples of dermatophytes in vitro. METHOD: Minimum inhibitory concentration of urea was determined for 31 samples of dermatophytes cultured in Sabouraud-dextrose broth containing different concentrations (7.5% up to 40%) of urea. Absence of growth was the criterion adopted to determine the minimum inhibitory concentration. RESULTS: The majority of samples (87%) were sensitive to urea at 12.5%, or less. 2 isolates of Trichophyton tonsurans and 2 of Trichophyton rubrum required 30%, and 40% urea, respectively, to be completely inhibited. CONCLUSION: In vitro results demonstrate inhibitory activity of urea on dermatophytes, suggesting that it could be used as an adjuvant in topical treatments. OBJETIVO: A uréia é comumente usada como substância queratolítica no tratamento das onicomicoses no intuito de melhorar a penetração das drogas antifúngicas. O objetivo deste estudo foi investigar a ação inibitória in vitro da uréia em amostras de dermatófitos MÉTODOS: A concentração inibitória mínima da uréia foi determinada para trinta e uma amostras de dermatófitos semeadas em meio de cultura Sabouraud-dextrose contendo diferentes concentrações (7,5% até 40%) de uréia. Ausência de crescimento foi o critério adotado para a determinação da concentração inibitória mínima. RESULTADOS: A maioria das amostras (87%) foi sensível à uréia em concentrações de 12,5% ou menos. Apenas dois isolados de Trichophyton tonsurans e dois de Trichophyton rubrum foram inibidos completamente na presença de 30% e 40% de uréia, respectivamente. CONCLUSÃO: Os resultados in vitro demonstraram atividade inibitória da uréia sobre os dermatófitos, sugerindo que possa ser usada como um adjuvante em tratamentos tópicos. Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo2006-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/clinics/article/view/1750010.1590/S1807-59322006000100003Clinics; v. 61 n. 1 (2006); 9-14 Clinics; Vol. 61 Núm. 1 (2006); 9-14 Clinics; Vol. 61 No. 1 (2006); 9-14 1980-53221807-5932reponame:Clinicsinstname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/clinics/article/view/17500/19557Martins, José Eduardo CostaCorim, Simone MiwaArriagada, Giovanna Letícia HernándezMelo, Natalina Takahashi deHeins, Elisabeth Mariainfo:eu-repo/semantics/openAccess2012-05-22T18:04:54Zoai:revistas.usp.br:article/17500Revistahttps://www.revistas.usp.br/clinicsPUBhttps://www.revistas.usp.br/clinics/oai||clinics@hc.fm.usp.br1980-53221807-5932opendoar:2012-05-22T18:04:54Clinics - Universidade de São Paulo (USP)false
dc.title.none.fl_str_mv In vitro sensitivity of dermatophytes to urea
Sensibilidade in vitro de dermatófitos à uréia
title In vitro sensitivity of dermatophytes to urea
spellingShingle In vitro sensitivity of dermatophytes to urea
Martins, José Eduardo Costa
Onicomicoses
Dermatomicoses
Dermatófitos
Uréia
Ação Inibitória
Onychomycoses
Dermatophytoses
Dermatophytes
Urea
Inhibitory Activity
title_short In vitro sensitivity of dermatophytes to urea
title_full In vitro sensitivity of dermatophytes to urea
title_fullStr In vitro sensitivity of dermatophytes to urea
title_full_unstemmed In vitro sensitivity of dermatophytes to urea
title_sort In vitro sensitivity of dermatophytes to urea
author Martins, José Eduardo Costa
author_facet Martins, José Eduardo Costa
Corim, Simone Miwa
Arriagada, Giovanna Letícia Hernández
Melo, Natalina Takahashi de
Heins, Elisabeth Maria
author_role author
author2 Corim, Simone Miwa
Arriagada, Giovanna Letícia Hernández
Melo, Natalina Takahashi de
Heins, Elisabeth Maria
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Martins, José Eduardo Costa
Corim, Simone Miwa
Arriagada, Giovanna Letícia Hernández
Melo, Natalina Takahashi de
Heins, Elisabeth Maria
dc.subject.por.fl_str_mv Onicomicoses
Dermatomicoses
Dermatófitos
Uréia
Ação Inibitória
Onychomycoses
Dermatophytoses
Dermatophytes
Urea
Inhibitory Activity
topic Onicomicoses
Dermatomicoses
Dermatófitos
Uréia
Ação Inibitória
Onychomycoses
Dermatophytoses
Dermatophytes
Urea
Inhibitory Activity
description OBJECTIVE: Urea is commonly used as a keratolytic substance in the treatment of onychomycoses to improve the penetration of antifungal drugs in the lesion sites. The aim of the present study was to investigate the inhibitory action of urea on samples of dermatophytes in vitro. METHOD: Minimum inhibitory concentration of urea was determined for 31 samples of dermatophytes cultured in Sabouraud-dextrose broth containing different concentrations (7.5% up to 40%) of urea. Absence of growth was the criterion adopted to determine the minimum inhibitory concentration. RESULTS: The majority of samples (87%) were sensitive to urea at 12.5%, or less. 2 isolates of Trichophyton tonsurans and 2 of Trichophyton rubrum required 30%, and 40% urea, respectively, to be completely inhibited. CONCLUSION: In vitro results demonstrate inhibitory activity of urea on dermatophytes, suggesting that it could be used as an adjuvant in topical treatments.
publishDate 2006
dc.date.none.fl_str_mv 2006-02-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/clinics/article/view/17500
10.1590/S1807-59322006000100003
url https://www.revistas.usp.br/clinics/article/view/17500
identifier_str_mv 10.1590/S1807-59322006000100003
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/clinics/article/view/17500/19557
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo
publisher.none.fl_str_mv Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo
dc.source.none.fl_str_mv Clinics; v. 61 n. 1 (2006); 9-14
Clinics; Vol. 61 Núm. 1 (2006); 9-14
Clinics; Vol. 61 No. 1 (2006); 9-14
1980-5322
1807-5932
reponame:Clinics
instname:Universidade de São Paulo (USP)
instacron:USP
instname_str Universidade de São Paulo (USP)
instacron_str USP
institution USP
reponame_str Clinics
collection Clinics
repository.name.fl_str_mv Clinics - Universidade de São Paulo (USP)
repository.mail.fl_str_mv ||clinics@hc.fm.usp.br
_version_ 1787713167697117184

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