Performance of the 13C-urea breath test for the diagnosis of H. pylori infection using a substrate synthesized in Brazil: A preliminary study

Detalhes bibliográficos
Autor(a) principal: Coelho, Luiz Gonzaga
Data de Publicação: 2018
Outros Autores: Sant'Ana, Carlos Roberto, Oliveira, Ricardo Brandt de, Cezar, Raíra César e, Araujo, Aline Cordeiro Campos de, Silva, Raisa Cristina Teodoro da, Trindade, Osmar Reni, Coelho, Maria Clara, Ferrioli, Eduardo, Bendassolli, José Albertino
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Clinics
Texto Completo: https://www.revistas.usp.br/clinics/article/view/146904
Resumo: OBJECTIVE: The 13C-urea breath test is the main non-invasive test for the diagnosis of Helicobacter pylori infection. The availability of this test throughout the country is limited, mainly due to the difficulty in obtaining the labeled isotope from abroad. Recently, researchers from the Nuclear Energy Center in Agriculture at the University of São Paulo (CENA/USP) succeeded in synthesizing 13C-enriched urea for Helicobacter pylori diagnosis. The aim of the study was to compare the performance of the 13C-urea breath test using 13C-urea acquired abroad with that of a test using 13C-urea synthesized in Brazil. METHOD: Sixty-four dyspeptic patients participated in the study (24 men and 40 women). Initially, the patients performed the 13C-urea breath test using the imported substrate (Euriso-Top, France). Seven to fourteen days later, all the patients repeated the test using the Brazilian substrate. The samples from both examinations were processed in an infrared isotope analyzer (IRIS, Wagner Analisen Technik, Germany), and all delta over baseline (DOB) [%] values above four were considered positive results. RESULTS: Twenty-seven patients (42%) exhibited negative results for Helicobacter pylori infection, and thirty-seven patients (58%) exhibited positive results when tested using the foreign substrate (gold standard). There was a 100% concordance regarding the presence or absence of infection when the gold standard results were compared with those obtained using the Brazilian substrate. CONCLUSIONS: Similar performance in the diagnosis of Helicobacter pylori infection was demonstrated when using the 13C-urea breath test with the Brazilian 13C-urea substrate and the test with the substrate produced abroad. This validation represents an important step toward increasing the availability of the 13C-urea breath test throughout the country, which will have a positive influence on the management of Helicobacter pylori infection.
id USP-19_f78f95e399e7ecb47fc41cf6555a44c4
oai_identifier_str oai:revistas.usp.br:article/146904
network_acronym_str USP-19
network_name_str Clinics
repository_id_str
spelling Performance of the 13C-urea breath test for the diagnosis of H. pylori infection using a substrate synthesized in Brazil: A preliminary study13C-urea breath test13C-ureaHelicobacter pyloriH. pylori diagnosisOBJECTIVE: The 13C-urea breath test is the main non-invasive test for the diagnosis of Helicobacter pylori infection. The availability of this test throughout the country is limited, mainly due to the difficulty in obtaining the labeled isotope from abroad. Recently, researchers from the Nuclear Energy Center in Agriculture at the University of São Paulo (CENA/USP) succeeded in synthesizing 13C-enriched urea for Helicobacter pylori diagnosis. The aim of the study was to compare the performance of the 13C-urea breath test using 13C-urea acquired abroad with that of a test using 13C-urea synthesized in Brazil. METHOD: Sixty-four dyspeptic patients participated in the study (24 men and 40 women). Initially, the patients performed the 13C-urea breath test using the imported substrate (Euriso-Top, France). Seven to fourteen days later, all the patients repeated the test using the Brazilian substrate. The samples from both examinations were processed in an infrared isotope analyzer (IRIS, Wagner Analisen Technik, Germany), and all delta over baseline (DOB) [%] values above four were considered positive results. RESULTS: Twenty-seven patients (42%) exhibited negative results for Helicobacter pylori infection, and thirty-seven patients (58%) exhibited positive results when tested using the foreign substrate (gold standard). There was a 100% concordance regarding the presence or absence of infection when the gold standard results were compared with those obtained using the Brazilian substrate. CONCLUSIONS: Similar performance in the diagnosis of Helicobacter pylori infection was demonstrated when using the 13C-urea breath test with the Brazilian 13C-urea substrate and the test with the substrate produced abroad. This validation represents an important step toward increasing the availability of the 13C-urea breath test throughout the country, which will have a positive influence on the management of Helicobacter pylori infection.Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo2018-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/clinics/article/view/14690410.6061/clinics/2018/e16-553Clinics; Vol. 73 (2018); e16-553Clinics; v. 73 (2018); e16-553Clinics; Vol. 73 (2018); e16-5531980-53221807-5932reponame:Clinicsinstname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/clinics/article/view/146904/140439Copyright (c) 2018 Clinicsinfo:eu-repo/semantics/openAccessCoelho, Luiz GonzagaSant'Ana, Carlos RobertoOliveira, Ricardo Brandt deCezar, Raíra César eAraujo, Aline Cordeiro Campos deSilva, Raisa Cristina Teodoro daTrindade, Osmar ReniCoelho, Maria ClaraFerrioli, EduardoBendassolli, José Albertino2019-05-14T11:48:50Zoai:revistas.usp.br:article/146904Revistahttps://www.revistas.usp.br/clinicsPUBhttps://www.revistas.usp.br/clinics/oai||clinics@hc.fm.usp.br1980-53221807-5932opendoar:2019-05-14T11:48:50Clinics - Universidade de São Paulo (USP)false
dc.title.none.fl_str_mv Performance of the 13C-urea breath test for the diagnosis of H. pylori infection using a substrate synthesized in Brazil: A preliminary study
title Performance of the 13C-urea breath test for the diagnosis of H. pylori infection using a substrate synthesized in Brazil: A preliminary study
spellingShingle Performance of the 13C-urea breath test for the diagnosis of H. pylori infection using a substrate synthesized in Brazil: A preliminary study
Coelho, Luiz Gonzaga
13C-urea breath test
13C-urea
Helicobacter pylori
H. pylori diagnosis
title_short Performance of the 13C-urea breath test for the diagnosis of H. pylori infection using a substrate synthesized in Brazil: A preliminary study
title_full Performance of the 13C-urea breath test for the diagnosis of H. pylori infection using a substrate synthesized in Brazil: A preliminary study
title_fullStr Performance of the 13C-urea breath test for the diagnosis of H. pylori infection using a substrate synthesized in Brazil: A preliminary study
title_full_unstemmed Performance of the 13C-urea breath test for the diagnosis of H. pylori infection using a substrate synthesized in Brazil: A preliminary study
title_sort Performance of the 13C-urea breath test for the diagnosis of H. pylori infection using a substrate synthesized in Brazil: A preliminary study
author Coelho, Luiz Gonzaga
author_facet Coelho, Luiz Gonzaga
Sant'Ana, Carlos Roberto
Oliveira, Ricardo Brandt de
Cezar, Raíra César e
Araujo, Aline Cordeiro Campos de
Silva, Raisa Cristina Teodoro da
Trindade, Osmar Reni
Coelho, Maria Clara
Ferrioli, Eduardo
Bendassolli, José Albertino
author_role author
author2 Sant'Ana, Carlos Roberto
Oliveira, Ricardo Brandt de
Cezar, Raíra César e
Araujo, Aline Cordeiro Campos de
Silva, Raisa Cristina Teodoro da
Trindade, Osmar Reni
Coelho, Maria Clara
Ferrioli, Eduardo
Bendassolli, José Albertino
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Coelho, Luiz Gonzaga
Sant'Ana, Carlos Roberto
Oliveira, Ricardo Brandt de
Cezar, Raíra César e
Araujo, Aline Cordeiro Campos de
Silva, Raisa Cristina Teodoro da
Trindade, Osmar Reni
Coelho, Maria Clara
Ferrioli, Eduardo
Bendassolli, José Albertino
dc.subject.por.fl_str_mv 13C-urea breath test
13C-urea
Helicobacter pylori
H. pylori diagnosis
topic 13C-urea breath test
13C-urea
Helicobacter pylori
H. pylori diagnosis
description OBJECTIVE: The 13C-urea breath test is the main non-invasive test for the diagnosis of Helicobacter pylori infection. The availability of this test throughout the country is limited, mainly due to the difficulty in obtaining the labeled isotope from abroad. Recently, researchers from the Nuclear Energy Center in Agriculture at the University of São Paulo (CENA/USP) succeeded in synthesizing 13C-enriched urea for Helicobacter pylori diagnosis. The aim of the study was to compare the performance of the 13C-urea breath test using 13C-urea acquired abroad with that of a test using 13C-urea synthesized in Brazil. METHOD: Sixty-four dyspeptic patients participated in the study (24 men and 40 women). Initially, the patients performed the 13C-urea breath test using the imported substrate (Euriso-Top, France). Seven to fourteen days later, all the patients repeated the test using the Brazilian substrate. The samples from both examinations were processed in an infrared isotope analyzer (IRIS, Wagner Analisen Technik, Germany), and all delta over baseline (DOB) [%] values above four were considered positive results. RESULTS: Twenty-seven patients (42%) exhibited negative results for Helicobacter pylori infection, and thirty-seven patients (58%) exhibited positive results when tested using the foreign substrate (gold standard). There was a 100% concordance regarding the presence or absence of infection when the gold standard results were compared with those obtained using the Brazilian substrate. CONCLUSIONS: Similar performance in the diagnosis of Helicobacter pylori infection was demonstrated when using the 13C-urea breath test with the Brazilian 13C-urea substrate and the test with the substrate produced abroad. This validation represents an important step toward increasing the availability of the 13C-urea breath test throughout the country, which will have a positive influence on the management of Helicobacter pylori infection.
publishDate 2018
dc.date.none.fl_str_mv 2018-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/clinics/article/view/146904
10.6061/clinics/2018/e16-553
url https://www.revistas.usp.br/clinics/article/view/146904
identifier_str_mv 10.6061/clinics/2018/e16-553
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/clinics/article/view/146904/140439
dc.rights.driver.fl_str_mv Copyright (c) 2018 Clinics
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2018 Clinics
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo
publisher.none.fl_str_mv Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo
dc.source.none.fl_str_mv Clinics; Vol. 73 (2018); e16-553
Clinics; v. 73 (2018); e16-553
Clinics; Vol. 73 (2018); e16-553
1980-5322
1807-5932
reponame:Clinics
instname:Universidade de São Paulo (USP)
instacron:USP
instname_str Universidade de São Paulo (USP)
instacron_str USP
institution USP
reponame_str Clinics
collection Clinics
repository.name.fl_str_mv Clinics - Universidade de São Paulo (USP)
repository.mail.fl_str_mv ||clinics@hc.fm.usp.br
_version_ 1800222763704647680