The use of the microtechnique for intratypic serodifferentiation of type 1 and 3 poliovirus strains

Detalhes bibliográficos
Autor(a) principal: Stewien, Klaus E.
Data de Publicação: 1977
Outros Autores: Lacerda, José P. G. de
Tipo de documento: Artigo
Idioma: por
Título da fonte: Revista de Saúde Pública
Texto Completo: https://www.revistas.usp.br/rsp/article/view/22828
Resumo: The microtechnique was compared to the conventional macrotechnique for intratypic serodifferentiation of 28 type 1 and 3 poliovirus strains by the method of comparison of neutralization indices. The results of the tests showed a high correlation between the neutralization indices - 0.931 and 0.971 after incubation periods of three and five days, respectively - and all the strains were equally identified by both techniques studied. It is concluded that the microtechnique can be used as an alternative of the macrotechnique for intratypic serodifferentiation of type 1 and 3 poliovirus strains, with the advantage of economy of media and reagents and even with a marked saving in both time and effort.
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spelling The use of the microtechnique for intratypic serodifferentiation of type 1 and 3 poliovirus strains Utilização da microtécnica para a identificação sorológica intratípica de estirpes de poliovírus dos tipos 1 e 3 PoliovírusMarcadores genéticosMicrotécnicaPoliovirusGenetic markersMicrotechnique The microtechnique was compared to the conventional macrotechnique for intratypic serodifferentiation of 28 type 1 and 3 poliovirus strains by the method of comparison of neutralization indices. The results of the tests showed a high correlation between the neutralization indices - 0.931 and 0.971 after incubation periods of three and five days, respectively - and all the strains were equally identified by both techniques studied. It is concluded that the microtechnique can be used as an alternative of the macrotechnique for intratypic serodifferentiation of type 1 and 3 poliovirus strains, with the advantage of economy of media and reagents and even with a marked saving in both time and effort. Foram estudadas de modo comparativo a macro e a microtécnica em provas de identificação sorológica intratípica de 28 estirpes de poliovírus dos tipos 1 e 3, segundo o método da "comparação dos índices de neutralização". Os resultados alcançados mostraram uma correlação elevada entre os índices de neutralização obtidos com ambas as técnicas empregadas - 0,931 e 0,971 após incubação de três e cinco dias, respectivamente - e correspondência total na identificação das estirpes examinadas. Conclui-se que a microtécnica pode ser utilizada em substituição da macrotécnica tradicional na identificação intratípica de poliovírus dos tipos 1 e 3, com, a vantagem da economia de meios e reagentes, e até mesmo com sensível redução de esforços humanos e tempo de execução das provas. Universidade de São Paulo. Faculdade de Saúde Pública1977-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/rsp/article/view/2282810.1590/S0034-89101977000100012Revista de Saúde Pública; Vol. 11 No. 1 (1977); 135-142 Revista de Saúde Pública; Vol. 11 Núm. 1 (1977); 135-142 Revista de Saúde Pública; v. 11 n. 1 (1977); 135-142 1518-87870034-8910reponame:Revista de Saúde Públicainstname:Universidade de São Paulo (USP)instacron:USPporhttps://www.revistas.usp.br/rsp/article/view/22828/24852Copyright (c) 2017 Revista de Saúde Públicainfo:eu-repo/semantics/openAccessStewien, Klaus E.Lacerda, José P. G. de2012-05-28T13:59:30Zoai:revistas.usp.br:article/22828Revistahttps://www.revistas.usp.br/rsp/indexONGhttps://www.revistas.usp.br/rsp/oairevsp@org.usp.br||revsp1@usp.br1518-87870034-8910opendoar:2012-05-28T13:59:30Revista de Saúde Pública - Universidade de São Paulo (USP)false
dc.title.none.fl_str_mv The use of the microtechnique for intratypic serodifferentiation of type 1 and 3 poliovirus strains
Utilização da microtécnica para a identificação sorológica intratípica de estirpes de poliovírus dos tipos 1 e 3
title The use of the microtechnique for intratypic serodifferentiation of type 1 and 3 poliovirus strains
spellingShingle The use of the microtechnique for intratypic serodifferentiation of type 1 and 3 poliovirus strains
Stewien, Klaus E.
Poliovírus
Marcadores genéticos
Microtécnica
Poliovirus
Genetic markers
Microtechnique
title_short The use of the microtechnique for intratypic serodifferentiation of type 1 and 3 poliovirus strains
title_full The use of the microtechnique for intratypic serodifferentiation of type 1 and 3 poliovirus strains
title_fullStr The use of the microtechnique for intratypic serodifferentiation of type 1 and 3 poliovirus strains
title_full_unstemmed The use of the microtechnique for intratypic serodifferentiation of type 1 and 3 poliovirus strains
title_sort The use of the microtechnique for intratypic serodifferentiation of type 1 and 3 poliovirus strains
author Stewien, Klaus E.
author_facet Stewien, Klaus E.
Lacerda, José P. G. de
author_role author
author2 Lacerda, José P. G. de
author2_role author
dc.contributor.author.fl_str_mv Stewien, Klaus E.
Lacerda, José P. G. de
dc.subject.por.fl_str_mv Poliovírus
Marcadores genéticos
Microtécnica
Poliovirus
Genetic markers
Microtechnique
topic Poliovírus
Marcadores genéticos
Microtécnica
Poliovirus
Genetic markers
Microtechnique
description The microtechnique was compared to the conventional macrotechnique for intratypic serodifferentiation of 28 type 1 and 3 poliovirus strains by the method of comparison of neutralization indices. The results of the tests showed a high correlation between the neutralization indices - 0.931 and 0.971 after incubation periods of three and five days, respectively - and all the strains were equally identified by both techniques studied. It is concluded that the microtechnique can be used as an alternative of the macrotechnique for intratypic serodifferentiation of type 1 and 3 poliovirus strains, with the advantage of economy of media and reagents and even with a marked saving in both time and effort.
publishDate 1977
dc.date.none.fl_str_mv 1977-03-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/rsp/article/view/22828
10.1590/S0034-89101977000100012
url https://www.revistas.usp.br/rsp/article/view/22828
identifier_str_mv 10.1590/S0034-89101977000100012
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://www.revistas.usp.br/rsp/article/view/22828/24852
dc.rights.driver.fl_str_mv Copyright (c) 2017 Revista de Saúde Pública
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2017 Revista de Saúde Pública
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Saúde Pública
publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Saúde Pública
dc.source.none.fl_str_mv Revista de Saúde Pública; Vol. 11 No. 1 (1977); 135-142
Revista de Saúde Pública; Vol. 11 Núm. 1 (1977); 135-142
Revista de Saúde Pública; v. 11 n. 1 (1977); 135-142
1518-8787
0034-8910
reponame:Revista de Saúde Pública
instname:Universidade de São Paulo (USP)
instacron:USP
instname_str Universidade de São Paulo (USP)
instacron_str USP
institution USP
reponame_str Revista de Saúde Pública
collection Revista de Saúde Pública
repository.name.fl_str_mv Revista de Saúde Pública - Universidade de São Paulo (USP)
repository.mail.fl_str_mv revsp@org.usp.br||revsp1@usp.br
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