Growth and differentiation "In vitro" of strains of Trypanosoma cruzi isolated from wild animals

Detalhes bibliográficos
Autor(a) principal: Mello, Dalva A.
Data de Publicação: 1980
Outros Autores: Borges, Monamaris M., Chiarini, Lúcia Helena
Tipo de documento: Artigo
Idioma: por
Título da fonte: Revista de Saúde Pública
Texto Completo: https://www.revistas.usp.br/rsp/article/view/23029
Resumo: In the course of this work, four strains of sylvatic Trypanosoma cruzi were studied in regard to their growth and differentiation in LIT medium. R52, R64, and R65 were isolated from individual Didelphis albiventris (Marsupialia) and M226 from Calomys callosus (Rodentia). The evolution of the numerical incresase and morphogenesis of the trypanosome populations in the culture was observed at regular intervals during a period of 312 hours. Differential counts were made of amastigotes, epimastigotes, and trypomastigotes in Neubauer chambers. Results showed that the four strains had characteristic behavior patterns. M226 increased the most and was followed by R65, R52, and then R64.
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spelling Growth and differentiation "In vitro" of strains of Trypanosoma cruzi isolated from wild animals Crescimento e diferenciação "In vitro" de cepas de Trypanosoma cruzi, isoladas de animais silvestres Trypanosoma cruzi^i1^scepas silvestDidelphis albiventrisCalomys callosusTrypanosoma cruzi^i2^swild straDidelphis albiventrisCalomys callosus In the course of this work, four strains of sylvatic Trypanosoma cruzi were studied in regard to their growth and differentiation in LIT medium. R52, R64, and R65 were isolated from individual Didelphis albiventris (Marsupialia) and M226 from Calomys callosus (Rodentia). The evolution of the numerical incresase and morphogenesis of the trypanosome populations in the culture was observed at regular intervals during a period of 312 hours. Differential counts were made of amastigotes, epimastigotes, and trypomastigotes in Neubauer chambers. Results showed that the four strains had characteristic behavior patterns. M226 increased the most and was followed by R65, R52, and then R64. Foram estudadas três cepas de T. cruzi isoladas de Didelphis albiventris (R52, R64 e R65) e uma isolada de Calomys callosus (M226), quanto ao comportamento "In vitro" no meio LIT. A evolução da população dos tripanossomas com relação ao crescimento e morfogênese foi acompanhada por um período de 13 dias (312 horas), em intervalos regulares. As contagens diferenciais, separando-se formas amastigotas, epimastigotas e tripomastigotas, foram realizadas na câmara de Neubauer. Os resultados obtidos levaram à conclusão de que as 4 cepas estudadas têm comportamento distintos. O melhor crescimento obtido ocorreu para a cepa M226, seguindo-se por ordem decrescente a R65, R52 e R64. Os picos das populações ocorreram como segue: M226, entre 192-264 horas; R65 entre 168-240 horas; R52 às 240 horas e R64 entre 264-312 horas. Universidade de São Paulo. Faculdade de Saúde Pública1980-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/rsp/article/view/2302910.1590/S0034-89101980000400012Revista de Saúde Pública; Vol. 14 No. 4 (1980); 569-581 Revista de Saúde Pública; Vol. 14 Núm. 4 (1980); 569-581 Revista de Saúde Pública; v. 14 n. 4 (1980); 569-581 1518-87870034-8910reponame:Revista de Saúde Públicainstname:Universidade de São Paulo (USP)instacron:USPporhttps://www.revistas.usp.br/rsp/article/view/23029/25054Copyright (c) 2017 Revista de Saúde Públicainfo:eu-repo/semantics/openAccessMello, Dalva A.Borges, Monamaris M.Chiarini, Lúcia Helena2012-05-28T14:39:56Zoai:revistas.usp.br:article/23029Revistahttps://www.revistas.usp.br/rsp/indexONGhttps://www.revistas.usp.br/rsp/oairevsp@org.usp.br||revsp1@usp.br1518-87870034-8910opendoar:2012-05-28T14:39:56Revista de Saúde Pública - Universidade de São Paulo (USP)false
dc.title.none.fl_str_mv Growth and differentiation "In vitro" of strains of Trypanosoma cruzi isolated from wild animals
Crescimento e diferenciação "In vitro" de cepas de Trypanosoma cruzi, isoladas de animais silvestres
title Growth and differentiation "In vitro" of strains of Trypanosoma cruzi isolated from wild animals
spellingShingle Growth and differentiation "In vitro" of strains of Trypanosoma cruzi isolated from wild animals
Mello, Dalva A.
Trypanosoma cruzi^i1^scepas silvest
Didelphis albiventris
Calomys callosus
Trypanosoma cruzi^i2^swild stra
Didelphis albiventris
Calomys callosus
title_short Growth and differentiation "In vitro" of strains of Trypanosoma cruzi isolated from wild animals
title_full Growth and differentiation "In vitro" of strains of Trypanosoma cruzi isolated from wild animals
title_fullStr Growth and differentiation "In vitro" of strains of Trypanosoma cruzi isolated from wild animals
title_full_unstemmed Growth and differentiation "In vitro" of strains of Trypanosoma cruzi isolated from wild animals
title_sort Growth and differentiation "In vitro" of strains of Trypanosoma cruzi isolated from wild animals
author Mello, Dalva A.
author_facet Mello, Dalva A.
Borges, Monamaris M.
Chiarini, Lúcia Helena
author_role author
author2 Borges, Monamaris M.
Chiarini, Lúcia Helena
author2_role author
author
dc.contributor.author.fl_str_mv Mello, Dalva A.
Borges, Monamaris M.
Chiarini, Lúcia Helena
dc.subject.por.fl_str_mv Trypanosoma cruzi^i1^scepas silvest
Didelphis albiventris
Calomys callosus
Trypanosoma cruzi^i2^swild stra
Didelphis albiventris
Calomys callosus
topic Trypanosoma cruzi^i1^scepas silvest
Didelphis albiventris
Calomys callosus
Trypanosoma cruzi^i2^swild stra
Didelphis albiventris
Calomys callosus
description In the course of this work, four strains of sylvatic Trypanosoma cruzi were studied in regard to their growth and differentiation in LIT medium. R52, R64, and R65 were isolated from individual Didelphis albiventris (Marsupialia) and M226 from Calomys callosus (Rodentia). The evolution of the numerical incresase and morphogenesis of the trypanosome populations in the culture was observed at regular intervals during a period of 312 hours. Differential counts were made of amastigotes, epimastigotes, and trypomastigotes in Neubauer chambers. Results showed that the four strains had characteristic behavior patterns. M226 increased the most and was followed by R65, R52, and then R64.
publishDate 1980
dc.date.none.fl_str_mv 1980-12-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/rsp/article/view/23029
10.1590/S0034-89101980000400012
url https://www.revistas.usp.br/rsp/article/view/23029
identifier_str_mv 10.1590/S0034-89101980000400012
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://www.revistas.usp.br/rsp/article/view/23029/25054
dc.rights.driver.fl_str_mv Copyright (c) 2017 Revista de Saúde Pública
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2017 Revista de Saúde Pública
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Saúde Pública
publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Saúde Pública
dc.source.none.fl_str_mv Revista de Saúde Pública; Vol. 14 No. 4 (1980); 569-581
Revista de Saúde Pública; Vol. 14 Núm. 4 (1980); 569-581
Revista de Saúde Pública; v. 14 n. 4 (1980); 569-581
1518-8787
0034-8910
reponame:Revista de Saúde Pública
instname:Universidade de São Paulo (USP)
instacron:USP
instname_str Universidade de São Paulo (USP)
instacron_str USP
institution USP
reponame_str Revista de Saúde Pública
collection Revista de Saúde Pública
repository.name.fl_str_mv Revista de Saúde Pública - Universidade de São Paulo (USP)
repository.mail.fl_str_mv revsp@org.usp.br||revsp1@usp.br
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