Scanning electron microscopy of eggs of Haemagogus leucocelaenus (Diptera: Culicidae)

Detalhes bibliográficos
Autor(a) principal: Alencar, Jeronimo
Data de Publicação: 2003
Outros Autores: Guimarães, Anthony Érico, Mello, Rubens Pinto, Lopes, Catarina Macedo, Dégallier, Nicolas, Santos-Mallet, Jacenir R
Tipo de documento: Artigo
Idioma: por
Título da fonte: Revista de Saúde Pública
Texto Completo: https://www.revistas.usp.br/rsp/article/view/31643
Resumo: OBJECTIVE: To observe morphological details of the eggs of Haemagogus (Conopostegus) leucocelaenus, seen for the first time via scanning electron microscopy (SEM), with morphometric analysis of the main structures. METHODS: Eggs of Hg. leucocelaenus were obtained from females captured in the Biological Reserve of Tinguá, State of Rio de Janeiro. Some of the eggs were kept for hatching and others underwent processing for scanning electron microscopy studies. Three eggs were submitted to morphometric analysis. The material was fixed in 2.5% glutaraldehyde and postfixed in 1% osmium tetroxide, both in 0.1M, pH 7.2 sodium cacodylate buffer, then dehydrated in ethanol and dried using the critical point method. This was then set up on metallic supports, covered with gold and observed using the Jeol 5310 scanning electron microscope. Measurements were made with the aid of the Semafore analysis software coupled to the electron microscope. RESULTS: The eggs presented elliptical outlines of approximately 574 mm in length and 169 mm in width, with an egg index (l/w ratio) of 3.39 mm. The exochorion was extremely regular and had ornamentation that was usually hexagonal but sometimes pentagonal. Tubercles were observed on the chorionic cells, symmetrically arranged in relation to the longitudinal axis. Inside the cells, there were smaller, individualized tubercles, some arranged peripherally and others grouped to a greater or lesser extent in the center. The surface of the chorionic reticulum did not present rugosity. The micropylar apparatus was formed by a prominent continuous collar of 8.32 mm in thickness, with a slightly irregular surface. The micropylar disk was very evident, and was continuous with the collar. The micropyle was seen at the center of this disk, measuring 1.6 mm and with a micropylar apparatus of 27.3 mm in diameter. CONCLUSIONS: The ornamentation of the exochorion presents differences in relation to the tubercles of chorionic cells and the external chorionic reticulum between the eggs of Hg. Leucocelaenus, in comparison with the eggs of Hg. janthinomys and Hg. equinus, and also in relation to those of Aedes aegypti, Ae. albopictus and Ae bahamensis. In various aspects, the eggs of Hg. leucocelaenus have more resemblance to those of Hg. Equinus than those of Hg. janthinomys, with greater differences presented in relation to the eggs of Hg. spegazzinii and Hg. lucifer.
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spelling Scanning electron microscopy of eggs of Haemagogus leucocelaenus (Diptera: Culicidae) Microscopia eletrônica de varredura de ovos de Haemagogus leucocelaenus (Diptera: Culicidae) Diptera^i2^sultrastructCulicidae^i2^sultrastructEggsMicroscopyelectronscanningHaemagogusDipteros^i1^sultra-estrutCulicidae^i1^sultra-estrutOvosMicroscopia eletrônica de varreduraHaemagogus OBJECTIVE: To observe morphological details of the eggs of Haemagogus (Conopostegus) leucocelaenus, seen for the first time via scanning electron microscopy (SEM), with morphometric analysis of the main structures. METHODS: Eggs of Hg. leucocelaenus were obtained from females captured in the Biological Reserve of Tinguá, State of Rio de Janeiro. Some of the eggs were kept for hatching and others underwent processing for scanning electron microscopy studies. Three eggs were submitted to morphometric analysis. The material was fixed in 2.5% glutaraldehyde and postfixed in 1% osmium tetroxide, both in 0.1M, pH 7.2 sodium cacodylate buffer, then dehydrated in ethanol and dried using the critical point method. This was then set up on metallic supports, covered with gold and observed using the Jeol 5310 scanning electron microscope. Measurements were made with the aid of the Semafore analysis software coupled to the electron microscope. RESULTS: The eggs presented elliptical outlines of approximately 574 mm in length and 169 mm in width, with an egg index (l/w ratio) of 3.39 mm. The exochorion was extremely regular and had ornamentation that was usually hexagonal but sometimes pentagonal. Tubercles were observed on the chorionic cells, symmetrically arranged in relation to the longitudinal axis. Inside the cells, there were smaller, individualized tubercles, some arranged peripherally and others grouped to a greater or lesser extent in the center. The surface of the chorionic reticulum did not present rugosity. The micropylar apparatus was formed by a prominent continuous collar of 8.32 mm in thickness, with a slightly irregular surface. The micropylar disk was very evident, and was continuous with the collar. The micropyle was seen at the center of this disk, measuring 1.6 mm and with a micropylar apparatus of 27.3 mm in diameter. CONCLUSIONS: The ornamentation of the exochorion presents differences in relation to the tubercles of chorionic cells and the external chorionic reticulum between the eggs of Hg. Leucocelaenus, in comparison with the eggs of Hg. janthinomys and Hg. equinus, and also in relation to those of Aedes aegypti, Ae. albopictus and Ae bahamensis. In various aspects, the eggs of Hg. leucocelaenus have more resemblance to those of Hg. Equinus than those of Hg. janthinomys, with greater differences presented in relation to the eggs of Hg. spegazzinii and Hg. lucifer. OBJETIVO: Observar detalhes morfológicos de ovos de Haemagogus leucocelaenus visualizados pela primeira vez por microscopia eletrônica de varredura (MEV) e realizar morfometria das principais estruturas. MÉTODOS: Foram utilizados ovos de Hg. leucocelaenus provenientes de fêmeas capturadas na Reserva Biológica do Tinguá, RJ, sendo parte destinada à eclosão e outra ao processamento de MEV, dos quais três foram submetidos à análise morfométrica. O material foi fixado em glutaraldeído 2,5% e pós-fixado em tetróxido de ósmio 1%, ambos em tampão cacodilato de sódio 0.1M, pH 7.2, processado e observado ao MEV Jeol 5310. Medições foram realizadas com o auxílio do software de análise Semafore. RESULTADOS: Os ovos apresentaram contorno elíptico com aproximadamente 574 µm de comprimento e 169 µm de largura, sendo o índice do ovo (l/wratio) 3,39 µm. O exocório é extremamente regular, possuindo ornamentação hexagonal e algumas vezes pentagonal. Nas células coriônicas, observaram-se tubérculos simetricamente dispostos com relação ao eixo longitudinal, e, no interior delas, tubérculos menores, individualizados, dispostos na periferia, e poucos agrupados no centro. A superfície do retículo coriônico não apresentou rugosidades. O aparelho micropilar apresenta colar proeminente, contínuo, com disco micropilar bem evidente. CONCLUSÕES: A ornamentação do exocório apresenta diferenças em relação aos tubérculos das células coriônicas e ao retículo coriônico externo entre os ovos de Hg. leucocelaenus comparados aos ovos de Hg. janthinomys e Hg. equinus, bem como com relação aos de Aedes aegypti, Ae. albopictus e Ae bahamensis. Universidade de São Paulo. Faculdade de Saúde Pública2003-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/rsp/article/view/3164310.1590/S0034-89102003000500017Revista de Saúde Pública; Vol. 37 No. 5 (2003); 658-661 Revista de Saúde Pública; Vol. 37 Núm. 5 (2003); 658-661 Revista de Saúde Pública; v. 37 n. 5 (2003); 658-661 1518-87870034-8910reponame:Revista de Saúde Públicainstname:Universidade de São Paulo (USP)instacron:USPporhttps://www.revistas.usp.br/rsp/article/view/31643/33529Copyright (c) 2017 Revista de Saúde Públicainfo:eu-repo/semantics/openAccessAlencar, JeronimoGuimarães, Anthony ÉricoMello, Rubens PintoLopes, Catarina MacedoDégallier, NicolasSantos-Mallet, Jacenir R2012-07-08T15:00:06Zoai:revistas.usp.br:article/31643Revistahttps://www.revistas.usp.br/rsp/indexONGhttps://www.revistas.usp.br/rsp/oairevsp@org.usp.br||revsp1@usp.br1518-87870034-8910opendoar:2012-07-08T15:00:06Revista de Saúde Pública - Universidade de São Paulo (USP)false
dc.title.none.fl_str_mv Scanning electron microscopy of eggs of Haemagogus leucocelaenus (Diptera: Culicidae)
Microscopia eletrônica de varredura de ovos de Haemagogus leucocelaenus (Diptera: Culicidae)
title Scanning electron microscopy of eggs of Haemagogus leucocelaenus (Diptera: Culicidae)
spellingShingle Scanning electron microscopy of eggs of Haemagogus leucocelaenus (Diptera: Culicidae)
Alencar, Jeronimo
Diptera^i2^sultrastruct
Culicidae^i2^sultrastruct
Eggs
Microscopy
electron
scanning
Haemagogus
Dipteros^i1^sultra-estrut
Culicidae^i1^sultra-estrut
Ovos
Microscopia eletrônica de varredura
Haemagogus
title_short Scanning electron microscopy of eggs of Haemagogus leucocelaenus (Diptera: Culicidae)
title_full Scanning electron microscopy of eggs of Haemagogus leucocelaenus (Diptera: Culicidae)
title_fullStr Scanning electron microscopy of eggs of Haemagogus leucocelaenus (Diptera: Culicidae)
title_full_unstemmed Scanning electron microscopy of eggs of Haemagogus leucocelaenus (Diptera: Culicidae)
title_sort Scanning electron microscopy of eggs of Haemagogus leucocelaenus (Diptera: Culicidae)
author Alencar, Jeronimo
author_facet Alencar, Jeronimo
Guimarães, Anthony Érico
Mello, Rubens Pinto
Lopes, Catarina Macedo
Dégallier, Nicolas
Santos-Mallet, Jacenir R
author_role author
author2 Guimarães, Anthony Érico
Mello, Rubens Pinto
Lopes, Catarina Macedo
Dégallier, Nicolas
Santos-Mallet, Jacenir R
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Alencar, Jeronimo
Guimarães, Anthony Érico
Mello, Rubens Pinto
Lopes, Catarina Macedo
Dégallier, Nicolas
Santos-Mallet, Jacenir R
dc.subject.por.fl_str_mv Diptera^i2^sultrastruct
Culicidae^i2^sultrastruct
Eggs
Microscopy
electron
scanning
Haemagogus
Dipteros^i1^sultra-estrut
Culicidae^i1^sultra-estrut
Ovos
Microscopia eletrônica de varredura
Haemagogus
topic Diptera^i2^sultrastruct
Culicidae^i2^sultrastruct
Eggs
Microscopy
electron
scanning
Haemagogus
Dipteros^i1^sultra-estrut
Culicidae^i1^sultra-estrut
Ovos
Microscopia eletrônica de varredura
Haemagogus
description OBJECTIVE: To observe morphological details of the eggs of Haemagogus (Conopostegus) leucocelaenus, seen for the first time via scanning electron microscopy (SEM), with morphometric analysis of the main structures. METHODS: Eggs of Hg. leucocelaenus were obtained from females captured in the Biological Reserve of Tinguá, State of Rio de Janeiro. Some of the eggs were kept for hatching and others underwent processing for scanning electron microscopy studies. Three eggs were submitted to morphometric analysis. The material was fixed in 2.5% glutaraldehyde and postfixed in 1% osmium tetroxide, both in 0.1M, pH 7.2 sodium cacodylate buffer, then dehydrated in ethanol and dried using the critical point method. This was then set up on metallic supports, covered with gold and observed using the Jeol 5310 scanning electron microscope. Measurements were made with the aid of the Semafore analysis software coupled to the electron microscope. RESULTS: The eggs presented elliptical outlines of approximately 574 mm in length and 169 mm in width, with an egg index (l/w ratio) of 3.39 mm. The exochorion was extremely regular and had ornamentation that was usually hexagonal but sometimes pentagonal. Tubercles were observed on the chorionic cells, symmetrically arranged in relation to the longitudinal axis. Inside the cells, there were smaller, individualized tubercles, some arranged peripherally and others grouped to a greater or lesser extent in the center. The surface of the chorionic reticulum did not present rugosity. The micropylar apparatus was formed by a prominent continuous collar of 8.32 mm in thickness, with a slightly irregular surface. The micropylar disk was very evident, and was continuous with the collar. The micropyle was seen at the center of this disk, measuring 1.6 mm and with a micropylar apparatus of 27.3 mm in diameter. CONCLUSIONS: The ornamentation of the exochorion presents differences in relation to the tubercles of chorionic cells and the external chorionic reticulum between the eggs of Hg. Leucocelaenus, in comparison with the eggs of Hg. janthinomys and Hg. equinus, and also in relation to those of Aedes aegypti, Ae. albopictus and Ae bahamensis. In various aspects, the eggs of Hg. leucocelaenus have more resemblance to those of Hg. Equinus than those of Hg. janthinomys, with greater differences presented in relation to the eggs of Hg. spegazzinii and Hg. lucifer.
publishDate 2003
dc.date.none.fl_str_mv 2003-10-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/rsp/article/view/31643
10.1590/S0034-89102003000500017
url https://www.revistas.usp.br/rsp/article/view/31643
identifier_str_mv 10.1590/S0034-89102003000500017
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://www.revistas.usp.br/rsp/article/view/31643/33529
dc.rights.driver.fl_str_mv Copyright (c) 2017 Revista de Saúde Pública
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2017 Revista de Saúde Pública
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Saúde Pública
publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Saúde Pública
dc.source.none.fl_str_mv Revista de Saúde Pública; Vol. 37 No. 5 (2003); 658-661
Revista de Saúde Pública; Vol. 37 Núm. 5 (2003); 658-661
Revista de Saúde Pública; v. 37 n. 5 (2003); 658-661
1518-8787
0034-8910
reponame:Revista de Saúde Pública
instname:Universidade de São Paulo (USP)
instacron:USP
instname_str Universidade de São Paulo (USP)
instacron_str USP
institution USP
reponame_str Revista de Saúde Pública
collection Revista de Saúde Pública
repository.name.fl_str_mv Revista de Saúde Pública - Universidade de São Paulo (USP)
repository.mail.fl_str_mv revsp@org.usp.br||revsp1@usp.br
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