β-lactamase-producing Gram-negative bacteria in an intensive care unit in southern Brazil

• Autor(a) principal: Leite, Clariana Akemi Kariya
• Data de Publicação: 2017
• Outros Autores: Oizumi, Karina Yoshimi, Caleffi-Ferracioli, Katiany Rizzieri, Scodro, Regiane Bertin de Lima, Pádua, Rubia Andreia Falleiros de, Cardoso, Rosilene Fressatti, Pires, Claudia Terencio Agostinho, Siqueira, Vera Lucia Dias
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Brazilian Journal of Pharmaceutical Sciences
• Texto Completo: https://www.revistas.usp.br/bjps/article/view/134154
• topic: Enterobacteriaceae; Pseudomonas aeruginosa; Acinetobacter spp; Antimicrobial resistance; Beta-lactamases; Bacterial typing; Intensive Care Unit/study/Brazil; Drug-resistant bacteria.

The present study evaluated the antimicrobial susceptibility profile, β-lactamase production, and genetic diversity of Enterobacteriaceae, Pseudomonas aeruginosa, and Acinetobacter spp. using phenotypic identification, antimicrobial susceptibility testing, and β-lactamase phenotypic detection. Isolates were obtained from patients in an intensive care unit in a hospital in southern Brazil. Bacterial genomic DNA was extracted, followed by the genotypic detection of carbapenemases and enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR). Fifty-six isolates (26 Klebsiella pneumoniae, five Escherichia coli, three Enterobacter aerogenes, nine P. aeruginosa, and 13 Acinetobacter spp.) were evaluated. The phenotypic extended spectrum β-lactamase (ESBL) test was positive in 53.8% of the K. pneumoniae isolates, 100.0% of the E. coli isolates, and 100.0% of the E. aerogenes isolates. Phenotypic and genotypic testing of K. pneumoniae carbapenemase (KPC) was positive in 50.0% of the K. pneumoniae isolates. Phenotypic and genotypic testing showed that none of the P. aeruginosa or Acinetobacter spp. isolates were positive for metallo- β-lactamase (MBL). The bla OXA gene was detected only in Acinetobacter spp. The lowest genetic diversity, determined by ERIC-PCR, was observed among the KPC-producing K. pneumoniae isolates and OXA-producing Acinetobacter spp. isolates, indicating the inadequate dissemination control of multidrug-resistant bacteria in this hospital environment.