Vasorelaxant activity and acute toxicity of the ethanolic extract of Zanthoxylum rhoifolium Lam leaves
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Publication Date: | 2019 |
Other Authors: | , , , , , , , , |
Format: | Article |
Language: | eng |
Source: | Brazilian Journal of Pharmaceutical Sciences |
Download full: | https://www.revistas.usp.br/bjps/article/view/164721 |
Summary: | The study evaluated the vasorelaxant effect induced by the ethanolic extract of the leaves of Zanthoxylum rhoifolium Lam (EEtOH-Zr/leaves). Wistar rats were treated with the leaf extract containing a single dose of 2,000 mg / kg, v.o. After 14 days, the animals were anesthetized for blood collection and subsequent analysis of the biochemical parameters; they were then euthanized (sodium pentobarbital-100 mg/kg, i.p.) for the removal and morphological analysis of the heart, lung, liver and kidney. The vasorelaxation activity the and vascular reactivity of EEtOH-Zr/leaves were evaluated on artery mesenteric rings isolated from rats. The extract showed no signs of toxicity and no significant difference in the values of the biochemical parameters between the control group and the group of treated animals. In the evaluation of pharmacological activity in the smooth muscle, the EEtOH-Zr/leaves caused vasorelaxant effect on the tonic contraction induced by phenylephrine in mesenteric artery preparations in the presence (pD2=2.17±0.05 µg/mL; Emax=99.8±5.2%) and absence (pD2=2.14±0.05 µg/mL; Emax=95.3±6.4%) of the vascular endothelium. Oral administration of EEtOH-Zr/leaves reduced the contraction induced by the cumulative addition of PHE. It is concluded that the EEtOH-Zr/leaves promote vasorelaxation and reduce vascular reactivity of adrenergic alpha-1 agonist in the mesenteric artery. The results did not show toxic effects of the extract. |
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Brazilian Journal of Pharmaceutical Sciences |
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Vasorelaxant activity and acute toxicity of the ethanolic extract of Zanthoxylum rhoifolium Lam leavesMesenteric arteries/ drug effectsVasorelaxantZanthoxylum rhoifolium/ toxicityRats, WistarBlood Vessels/ drug effectsThe study evaluated the vasorelaxant effect induced by the ethanolic extract of the leaves of Zanthoxylum rhoifolium Lam (EEtOH-Zr/leaves). Wistar rats were treated with the leaf extract containing a single dose of 2,000 mg / kg, v.o. After 14 days, the animals were anesthetized for blood collection and subsequent analysis of the biochemical parameters; they were then euthanized (sodium pentobarbital-100 mg/kg, i.p.) for the removal and morphological analysis of the heart, lung, liver and kidney. The vasorelaxation activity the and vascular reactivity of EEtOH-Zr/leaves were evaluated on artery mesenteric rings isolated from rats. The extract showed no signs of toxicity and no significant difference in the values of the biochemical parameters between the control group and the group of treated animals. In the evaluation of pharmacological activity in the smooth muscle, the EEtOH-Zr/leaves caused vasorelaxant effect on the tonic contraction induced by phenylephrine in mesenteric artery preparations in the presence (pD2=2.17±0.05 µg/mL; Emax=99.8±5.2%) and absence (pD2=2.14±0.05 µg/mL; Emax=95.3±6.4%) of the vascular endothelium. Oral administration of EEtOH-Zr/leaves reduced the contraction induced by the cumulative addition of PHE. It is concluded that the EEtOH-Zr/leaves promote vasorelaxation and reduce vascular reactivity of adrenergic alpha-1 agonist in the mesenteric artery. The results did not show toxic effects of the extract.Universidade de São Paulo. Faculdade de Ciências Farmacêuticas2019-12-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/bjps/article/view/16472110.1590/s2175-97902019000117754Brazilian Journal of Pharmaceutical Sciences; Vol. 55 (2019); e17754Brazilian Journal of Pharmaceutical Sciences; v. 55 (2019); e17754Brazilian Journal of Pharmaceutical Sciences; Vol. 55 (2019); e177542175-97901984-8250reponame:Brazilian Journal of Pharmaceutical Sciencesinstname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/bjps/article/view/164721/157933Copyright (c) 2019 Brazilian Journal of Pharmaceutical Scienceshttp://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessSilva, Joliane CarvalhoAzevedo, Pedro Simão da SilvaSouza, Fabiana de MouraAragão, Kalynca Kayla VianaSabino, Carla Kelly BarrosoCarvalho, Gabriela DantasMorais, Ilmara Cecília PinheiroMendes, Marcelo BezerraChaves, Mariana HelenaOliveira, Aldeídia Pereira de2019-12-02T18:32:20Zoai:revistas.usp.br:article/164721Revistahttps://www.revistas.usp.br/bjps/indexPUBhttps://old.scielo.br/oai/scielo-oai.phpbjps@usp.br||elizabeth.igne@gmail.com2175-97901984-8250opendoar:2019-12-02T18:32:20Brazilian Journal of Pharmaceutical Sciences - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Vasorelaxant activity and acute toxicity of the ethanolic extract of Zanthoxylum rhoifolium Lam leaves |
title |
Vasorelaxant activity and acute toxicity of the ethanolic extract of Zanthoxylum rhoifolium Lam leaves |
spellingShingle |
Vasorelaxant activity and acute toxicity of the ethanolic extract of Zanthoxylum rhoifolium Lam leaves Silva, Joliane Carvalho Mesenteric arteries/ drug effects Vasorelaxant Zanthoxylum rhoifolium/ toxicity Rats, Wistar Blood Vessels/ drug effects |
title_short |
Vasorelaxant activity and acute toxicity of the ethanolic extract of Zanthoxylum rhoifolium Lam leaves |
title_full |
Vasorelaxant activity and acute toxicity of the ethanolic extract of Zanthoxylum rhoifolium Lam leaves |
title_fullStr |
Vasorelaxant activity and acute toxicity of the ethanolic extract of Zanthoxylum rhoifolium Lam leaves |
title_full_unstemmed |
Vasorelaxant activity and acute toxicity of the ethanolic extract of Zanthoxylum rhoifolium Lam leaves |
title_sort |
Vasorelaxant activity and acute toxicity of the ethanolic extract of Zanthoxylum rhoifolium Lam leaves |
author |
Silva, Joliane Carvalho |
author_facet |
Silva, Joliane Carvalho Azevedo, Pedro Simão da Silva Souza, Fabiana de Moura Aragão, Kalynca Kayla Viana Sabino, Carla Kelly Barroso Carvalho, Gabriela Dantas Morais, Ilmara Cecília Pinheiro Mendes, Marcelo Bezerra Chaves, Mariana Helena Oliveira, Aldeídia Pereira de |
author_role |
author |
author2 |
Azevedo, Pedro Simão da Silva Souza, Fabiana de Moura Aragão, Kalynca Kayla Viana Sabino, Carla Kelly Barroso Carvalho, Gabriela Dantas Morais, Ilmara Cecília Pinheiro Mendes, Marcelo Bezerra Chaves, Mariana Helena Oliveira, Aldeídia Pereira de |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Silva, Joliane Carvalho Azevedo, Pedro Simão da Silva Souza, Fabiana de Moura Aragão, Kalynca Kayla Viana Sabino, Carla Kelly Barroso Carvalho, Gabriela Dantas Morais, Ilmara Cecília Pinheiro Mendes, Marcelo Bezerra Chaves, Mariana Helena Oliveira, Aldeídia Pereira de |
dc.subject.por.fl_str_mv |
Mesenteric arteries/ drug effects Vasorelaxant Zanthoxylum rhoifolium/ toxicity Rats, Wistar Blood Vessels/ drug effects |
topic |
Mesenteric arteries/ drug effects Vasorelaxant Zanthoxylum rhoifolium/ toxicity Rats, Wistar Blood Vessels/ drug effects |
description |
The study evaluated the vasorelaxant effect induced by the ethanolic extract of the leaves of Zanthoxylum rhoifolium Lam (EEtOH-Zr/leaves). Wistar rats were treated with the leaf extract containing a single dose of 2,000 mg / kg, v.o. After 14 days, the animals were anesthetized for blood collection and subsequent analysis of the biochemical parameters; they were then euthanized (sodium pentobarbital-100 mg/kg, i.p.) for the removal and morphological analysis of the heart, lung, liver and kidney. The vasorelaxation activity the and vascular reactivity of EEtOH-Zr/leaves were evaluated on artery mesenteric rings isolated from rats. The extract showed no signs of toxicity and no significant difference in the values of the biochemical parameters between the control group and the group of treated animals. In the evaluation of pharmacological activity in the smooth muscle, the EEtOH-Zr/leaves caused vasorelaxant effect on the tonic contraction induced by phenylephrine in mesenteric artery preparations in the presence (pD2=2.17±0.05 µg/mL; Emax=99.8±5.2%) and absence (pD2=2.14±0.05 µg/mL; Emax=95.3±6.4%) of the vascular endothelium. Oral administration of EEtOH-Zr/leaves reduced the contraction induced by the cumulative addition of PHE. It is concluded that the EEtOH-Zr/leaves promote vasorelaxation and reduce vascular reactivity of adrenergic alpha-1 agonist in the mesenteric artery. The results did not show toxic effects of the extract. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-12-02 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/bjps/article/view/164721 10.1590/s2175-97902019000117754 |
url |
https://www.revistas.usp.br/bjps/article/view/164721 |
identifier_str_mv |
10.1590/s2175-97902019000117754 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/bjps/article/view/164721/157933 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2019 Brazilian Journal of Pharmaceutical Sciences http://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2019 Brazilian Journal of Pharmaceutical Sciences http://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Ciências Farmacêuticas |
publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Ciências Farmacêuticas |
dc.source.none.fl_str_mv |
Brazilian Journal of Pharmaceutical Sciences; Vol. 55 (2019); e17754 Brazilian Journal of Pharmaceutical Sciences; v. 55 (2019); e17754 Brazilian Journal of Pharmaceutical Sciences; Vol. 55 (2019); e17754 2175-9790 1984-8250 reponame:Brazilian Journal of Pharmaceutical Sciences instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Brazilian Journal of Pharmaceutical Sciences |
collection |
Brazilian Journal of Pharmaceutical Sciences |
repository.name.fl_str_mv |
Brazilian Journal of Pharmaceutical Sciences - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
bjps@usp.br||elizabeth.igne@gmail.com |
_version_ |
1787713371839135744 |