Polymeric micelles containing resveratrol: development, characterization, cytotoxicity on tumor cells and antimicrobial activity

Detalhes bibliográficos
Autor(a) principal: Almeida, Tamires Cunha
Data de Publicação: 2020
Outros Autores: Seibert, Janaina Brandão, Almeida, Sávio Henrique de Souza, Amparo, Tatiane Roquete, Teixeira, Luiz Fernando de Medeiros, Barichello, Jose Mario, Postacchini, Bruna Bueno, Santos, Orlando David Henrique dos, Silva, Glenda Nicioli da
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Pharmaceutical Sciences
Texto Completo: https://www.revistas.usp.br/bjps/article/view/181829
Resumo: Antimicrobial and antitumor activities of resveratrol, a compound found mainly in grapes, have already been demonstrated. However, its low bioavailability is a limiting factor for therapeutic application. Polymeric micelles can be an approach to solve this problem since they can encapsulate hydrophobic substances. We developed and characterized micellar formulations containing resveratrol and evaluated their cytotoxic and antimicrobial effects. The formulations were prepared by the cold dispersion method with different concentrations of F127 (5 or 10% w/w) and resveratrol (500 or 5000 µM). The formulations were characterized according to size, polydispersity index, pH, encapsulation rate and in vitro release. Cytotoxic effect was evaluated on a bladder cancer cell line and antimicrobial effect was evaluated on E. coli, S. aureus and C. albicans. One of the formulations (10% w/w of F127 and 5000 µM of resveratrol) was a monodispersed solution with high encapsulation rate, thus it was chosen for the cytotoxicity and antimicrobial assays. MS10+RES-3 was able to preserve the antimicrobial and cytotoxic activity of resveratrol. This is the first study that evaluated antimicrobial potential and cytotoxicity of micelles containing resveratrol on bladder cancer cells and the results showed that micellar nanostructures could ensure the maintenance of the biological activity of resveratrol.
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spelling Polymeric micelles containing resveratrol: development, characterization, cytotoxicity on tumor cells and antimicrobial activityAntimicrobial activityCytotoxic activityF127®Polymeric micellesResveratrolAntimicrobial and antitumor activities of resveratrol, a compound found mainly in grapes, have already been demonstrated. However, its low bioavailability is a limiting factor for therapeutic application. Polymeric micelles can be an approach to solve this problem since they can encapsulate hydrophobic substances. We developed and characterized micellar formulations containing resveratrol and evaluated their cytotoxic and antimicrobial effects. The formulations were prepared by the cold dispersion method with different concentrations of F127 (5 or 10% w/w) and resveratrol (500 or 5000 µM). The formulations were characterized according to size, polydispersity index, pH, encapsulation rate and in vitro release. Cytotoxic effect was evaluated on a bladder cancer cell line and antimicrobial effect was evaluated on E. coli, S. aureus and C. albicans. One of the formulations (10% w/w of F127 and 5000 µM of resveratrol) was a monodispersed solution with high encapsulation rate, thus it was chosen for the cytotoxicity and antimicrobial assays. MS10+RES-3 was able to preserve the antimicrobial and cytotoxic activity of resveratrol. This is the first study that evaluated antimicrobial potential and cytotoxicity of micelles containing resveratrol on bladder cancer cells and the results showed that micellar nanostructures could ensure the maintenance of the biological activity of resveratrol.Universidade de São Paulo. Faculdade de Ciências Farmacêuticas2020-12-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/bjps/article/view/18182910.1590/s2175-97902019000418401Brazilian Journal of Pharmaceutical Sciences; Vol. 56 (2020); e18411Brazilian Journal of Pharmaceutical Sciences; v. 56 (2020); e18411Brazilian Journal of Pharmaceutical Sciences; Vol. 56 (2020); e184112175-97901984-8250reponame:Brazilian Journal of Pharmaceutical Sciencesinstname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/bjps/article/view/181829/168732Copyright (c) 2020 Brazilian Journal of Pharmaceutical Scienceshttp://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessAlmeida, Tamires Cunha Seibert, Janaina Brandão Almeida, Sávio Henrique de Souza Amparo, Tatiane Roquete Teixeira, Luiz Fernando de Medeiros Barichello, Jose Mario Postacchini, Bruna Bueno Santos, Orlando David Henrique dos Silva, Glenda Nicioli da 2021-06-12T19:46:54Zoai:revistas.usp.br:article/181829Revistahttps://www.revistas.usp.br/bjps/indexPUBhttps://old.scielo.br/oai/scielo-oai.phpbjps@usp.br||elizabeth.igne@gmail.com2175-97901984-8250opendoar:2021-06-12T19:46:54Brazilian Journal of Pharmaceutical Sciences - Universidade de São Paulo (USP)false
dc.title.none.fl_str_mv Polymeric micelles containing resveratrol: development, characterization, cytotoxicity on tumor cells and antimicrobial activity
title Polymeric micelles containing resveratrol: development, characterization, cytotoxicity on tumor cells and antimicrobial activity
spellingShingle Polymeric micelles containing resveratrol: development, characterization, cytotoxicity on tumor cells and antimicrobial activity
Almeida, Tamires Cunha
Antimicrobial activity
Cytotoxic activity
F127®
Polymeric micelles
Resveratrol
title_short Polymeric micelles containing resveratrol: development, characterization, cytotoxicity on tumor cells and antimicrobial activity
title_full Polymeric micelles containing resveratrol: development, characterization, cytotoxicity on tumor cells and antimicrobial activity
title_fullStr Polymeric micelles containing resveratrol: development, characterization, cytotoxicity on tumor cells and antimicrobial activity
title_full_unstemmed Polymeric micelles containing resveratrol: development, characterization, cytotoxicity on tumor cells and antimicrobial activity
title_sort Polymeric micelles containing resveratrol: development, characterization, cytotoxicity on tumor cells and antimicrobial activity
author Almeida, Tamires Cunha
author_facet Almeida, Tamires Cunha
Seibert, Janaina Brandão
Almeida, Sávio Henrique de Souza
Amparo, Tatiane Roquete
Teixeira, Luiz Fernando de Medeiros
Barichello, Jose Mario
Postacchini, Bruna Bueno
Santos, Orlando David Henrique dos
Silva, Glenda Nicioli da
author_role author
author2 Seibert, Janaina Brandão
Almeida, Sávio Henrique de Souza
Amparo, Tatiane Roquete
Teixeira, Luiz Fernando de Medeiros
Barichello, Jose Mario
Postacchini, Bruna Bueno
Santos, Orlando David Henrique dos
Silva, Glenda Nicioli da
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Almeida, Tamires Cunha
Seibert, Janaina Brandão
Almeida, Sávio Henrique de Souza
Amparo, Tatiane Roquete
Teixeira, Luiz Fernando de Medeiros
Barichello, Jose Mario
Postacchini, Bruna Bueno
Santos, Orlando David Henrique dos
Silva, Glenda Nicioli da
dc.subject.por.fl_str_mv Antimicrobial activity
Cytotoxic activity
F127®
Polymeric micelles
Resveratrol
topic Antimicrobial activity
Cytotoxic activity
F127®
Polymeric micelles
Resveratrol
description Antimicrobial and antitumor activities of resveratrol, a compound found mainly in grapes, have already been demonstrated. However, its low bioavailability is a limiting factor for therapeutic application. Polymeric micelles can be an approach to solve this problem since they can encapsulate hydrophobic substances. We developed and characterized micellar formulations containing resveratrol and evaluated their cytotoxic and antimicrobial effects. The formulations were prepared by the cold dispersion method with different concentrations of F127 (5 or 10% w/w) and resveratrol (500 or 5000 µM). The formulations were characterized according to size, polydispersity index, pH, encapsulation rate and in vitro release. Cytotoxic effect was evaluated on a bladder cancer cell line and antimicrobial effect was evaluated on E. coli, S. aureus and C. albicans. One of the formulations (10% w/w of F127 and 5000 µM of resveratrol) was a monodispersed solution with high encapsulation rate, thus it was chosen for the cytotoxicity and antimicrobial assays. MS10+RES-3 was able to preserve the antimicrobial and cytotoxic activity of resveratrol. This is the first study that evaluated antimicrobial potential and cytotoxicity of micelles containing resveratrol on bladder cancer cells and the results showed that micellar nanostructures could ensure the maintenance of the biological activity of resveratrol.
publishDate 2020
dc.date.none.fl_str_mv 2020-12-09
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/bjps/article/view/181829
10.1590/s2175-97902019000418401
url https://www.revistas.usp.br/bjps/article/view/181829
identifier_str_mv 10.1590/s2175-97902019000418401
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/bjps/article/view/181829/168732
dc.rights.driver.fl_str_mv Copyright (c) 2020 Brazilian Journal of Pharmaceutical Sciences
http://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2020 Brazilian Journal of Pharmaceutical Sciences
http://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Ciências Farmacêuticas
publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Ciências Farmacêuticas
dc.source.none.fl_str_mv Brazilian Journal of Pharmaceutical Sciences; Vol. 56 (2020); e18411
Brazilian Journal of Pharmaceutical Sciences; v. 56 (2020); e18411
Brazilian Journal of Pharmaceutical Sciences; Vol. 56 (2020); e18411
2175-9790
1984-8250
reponame:Brazilian Journal of Pharmaceutical Sciences
instname:Universidade de São Paulo (USP)
instacron:USP
instname_str Universidade de São Paulo (USP)
instacron_str USP
institution USP
reponame_str Brazilian Journal of Pharmaceutical Sciences
collection Brazilian Journal of Pharmaceutical Sciences
repository.name.fl_str_mv Brazilian Journal of Pharmaceutical Sciences - Universidade de São Paulo (USP)
repository.mail.fl_str_mv bjps@usp.br||elizabeth.igne@gmail.com
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