Fast detection of Mycobacterium tuberculosis in culture-positive sputum samples by nitrate reductase activity
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Pharmaceutical Sciences |
Texto Completo: | https://www.revistas.usp.br/bjps/article/view/146818 |
Resumo: | Microscopy and bacterial culture are the main tools in the diagnosis of tuberculosis. Since the slow growth of Mycobacterium tuberculosis impairs rapid diagnosis strategies, especially in countries where the latter are the only available resources, the ongoing development of new and inexpensive tools based on mycobacterial metabolism optimizing growth detection with preliminary identification is greatly welcome. When compared to the other species from the M. tuberculosis complex, M. tuberculosis is a strong nitrate reducer. Current assay compares the nitrate reductase activity of M. tuberculosis from pulmonary specimens cultivated in nitrate-supplemented media. Fifty-five sputum samples were decontaminated and inoculated in conventional (Middlebrook 7H9, Ogawa Kudoh-OK) and in nitrate-supplemented media (Middlebrook 7H9-N, Ogawa Kudoh-N). An aliquot from the media directly reacted with Griess reagent (7H9-N and OK-N) every five days, or transferred to a nitrate substrate solution (7H9, OK). Nitrate to nitrite reduction was considered positive, revealed by the pink color, indicating bacterial growth. As reference method, the Mycobacteria Growth Indicator Tube (MGIT) was used for sensitivity calculations and statistical analysis. 7H9-N and OK-N assays proved to perform better in detecting M. tuberculosis than conventional assays (7H9 and OK). Indeed, broth nitrate-supplemented medium (7H9-N) was comparable to MGIT to detect M. tuberculosis, except in growth detection time. Results show that 7H9-N may be used as an alternative tool particularly in low-income countries since it is a simple and cheap technique, and does not restrict diagnosis to single-source products. |
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Brazilian Journal of Pharmaceutical Sciences |
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Fast detection of Mycobacterium tuberculosis in culture-positive sputum samples by nitrate reductase activityTuberculosisMycobacteria/diagnosis/identificationNitrate reductase Microscopy and bacterial culture are the main tools in the diagnosis of tuberculosis. Since the slow growth of Mycobacterium tuberculosis impairs rapid diagnosis strategies, especially in countries where the latter are the only available resources, the ongoing development of new and inexpensive tools based on mycobacterial metabolism optimizing growth detection with preliminary identification is greatly welcome. When compared to the other species from the M. tuberculosis complex, M. tuberculosis is a strong nitrate reducer. Current assay compares the nitrate reductase activity of M. tuberculosis from pulmonary specimens cultivated in nitrate-supplemented media. Fifty-five sputum samples were decontaminated and inoculated in conventional (Middlebrook 7H9, Ogawa Kudoh-OK) and in nitrate-supplemented media (Middlebrook 7H9-N, Ogawa Kudoh-N). An aliquot from the media directly reacted with Griess reagent (7H9-N and OK-N) every five days, or transferred to a nitrate substrate solution (7H9, OK). Nitrate to nitrite reduction was considered positive, revealed by the pink color, indicating bacterial growth. As reference method, the Mycobacteria Growth Indicator Tube (MGIT) was used for sensitivity calculations and statistical analysis. 7H9-N and OK-N assays proved to perform better in detecting M. tuberculosis than conventional assays (7H9 and OK). Indeed, broth nitrate-supplemented medium (7H9-N) was comparable to MGIT to detect M. tuberculosis, except in growth detection time. Results show that 7H9-N may be used as an alternative tool particularly in low-income countries since it is a simple and cheap technique, and does not restrict diagnosis to single-source products.Universidade de São Paulo. Faculdade de Ciências Farmacêuticas2018-06-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/bjps/article/view/14681810.1590/s2175-97902018000100014Brazilian Journal of Pharmaceutical Sciences; Vol. 54 Núm. 1 (2018); e00014Brazilian Journal of Pharmaceutical Sciences; v. 54 n. 1 (2018); e00014Brazilian Journal of Pharmaceutical Sciences; Vol. 54 No. 1 (2018); e000142175-97901984-8250reponame:Brazilian Journal of Pharmaceutical Sciencesinstname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/bjps/article/view/146818/140347Copyright (c) 2018 Brazilian Journal of Pharmaceutical Sciences (Impresso)info:eu-repo/semantics/openAccessMeneguello, Jean EduardoOliveira, João VitorLima, Letícia SilvaSiqueira, Vera Lucia DiasScodro, Regiane Bertin de LimaCaleffi-Ferracioli, Katiany RizzieriCardoso, Rosilene Fressatti2018-06-07T16:31:56Zoai:revistas.usp.br:article/146818Revistahttps://www.revistas.usp.br/bjps/indexPUBhttps://old.scielo.br/oai/scielo-oai.phpbjps@usp.br||elizabeth.igne@gmail.com2175-97901984-8250opendoar:2018-06-07T16:31:56Brazilian Journal of Pharmaceutical Sciences - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Fast detection of Mycobacterium tuberculosis in culture-positive sputum samples by nitrate reductase activity |
title |
Fast detection of Mycobacterium tuberculosis in culture-positive sputum samples by nitrate reductase activity |
spellingShingle |
Fast detection of Mycobacterium tuberculosis in culture-positive sputum samples by nitrate reductase activity Meneguello, Jean Eduardo Tuberculosis Mycobacteria/diagnosis/identification Nitrate reductase |
title_short |
Fast detection of Mycobacterium tuberculosis in culture-positive sputum samples by nitrate reductase activity |
title_full |
Fast detection of Mycobacterium tuberculosis in culture-positive sputum samples by nitrate reductase activity |
title_fullStr |
Fast detection of Mycobacterium tuberculosis in culture-positive sputum samples by nitrate reductase activity |
title_full_unstemmed |
Fast detection of Mycobacterium tuberculosis in culture-positive sputum samples by nitrate reductase activity |
title_sort |
Fast detection of Mycobacterium tuberculosis in culture-positive sputum samples by nitrate reductase activity |
author |
Meneguello, Jean Eduardo |
author_facet |
Meneguello, Jean Eduardo Oliveira, João Vitor Lima, Letícia Silva Siqueira, Vera Lucia Dias Scodro, Regiane Bertin de Lima Caleffi-Ferracioli, Katiany Rizzieri Cardoso, Rosilene Fressatti |
author_role |
author |
author2 |
Oliveira, João Vitor Lima, Letícia Silva Siqueira, Vera Lucia Dias Scodro, Regiane Bertin de Lima Caleffi-Ferracioli, Katiany Rizzieri Cardoso, Rosilene Fressatti |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Meneguello, Jean Eduardo Oliveira, João Vitor Lima, Letícia Silva Siqueira, Vera Lucia Dias Scodro, Regiane Bertin de Lima Caleffi-Ferracioli, Katiany Rizzieri Cardoso, Rosilene Fressatti |
dc.subject.por.fl_str_mv |
Tuberculosis Mycobacteria/diagnosis/identification Nitrate reductase |
topic |
Tuberculosis Mycobacteria/diagnosis/identification Nitrate reductase |
description |
Microscopy and bacterial culture are the main tools in the diagnosis of tuberculosis. Since the slow growth of Mycobacterium tuberculosis impairs rapid diagnosis strategies, especially in countries where the latter are the only available resources, the ongoing development of new and inexpensive tools based on mycobacterial metabolism optimizing growth detection with preliminary identification is greatly welcome. When compared to the other species from the M. tuberculosis complex, M. tuberculosis is a strong nitrate reducer. Current assay compares the nitrate reductase activity of M. tuberculosis from pulmonary specimens cultivated in nitrate-supplemented media. Fifty-five sputum samples were decontaminated and inoculated in conventional (Middlebrook 7H9, Ogawa Kudoh-OK) and in nitrate-supplemented media (Middlebrook 7H9-N, Ogawa Kudoh-N). An aliquot from the media directly reacted with Griess reagent (7H9-N and OK-N) every five days, or transferred to a nitrate substrate solution (7H9, OK). Nitrate to nitrite reduction was considered positive, revealed by the pink color, indicating bacterial growth. As reference method, the Mycobacteria Growth Indicator Tube (MGIT) was used for sensitivity calculations and statistical analysis. 7H9-N and OK-N assays proved to perform better in detecting M. tuberculosis than conventional assays (7H9 and OK). Indeed, broth nitrate-supplemented medium (7H9-N) was comparable to MGIT to detect M. tuberculosis, except in growth detection time. Results show that 7H9-N may be used as an alternative tool particularly in low-income countries since it is a simple and cheap technique, and does not restrict diagnosis to single-source products. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-06-07 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/bjps/article/view/146818 10.1590/s2175-97902018000100014 |
url |
https://www.revistas.usp.br/bjps/article/view/146818 |
identifier_str_mv |
10.1590/s2175-97902018000100014 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/bjps/article/view/146818/140347 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2018 Brazilian Journal of Pharmaceutical Sciences (Impresso) info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2018 Brazilian Journal of Pharmaceutical Sciences (Impresso) |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Ciências Farmacêuticas |
publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Ciências Farmacêuticas |
dc.source.none.fl_str_mv |
Brazilian Journal of Pharmaceutical Sciences; Vol. 54 Núm. 1 (2018); e00014 Brazilian Journal of Pharmaceutical Sciences; v. 54 n. 1 (2018); e00014 Brazilian Journal of Pharmaceutical Sciences; Vol. 54 No. 1 (2018); e00014 2175-9790 1984-8250 reponame:Brazilian Journal of Pharmaceutical Sciences instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Brazilian Journal of Pharmaceutical Sciences |
collection |
Brazilian Journal of Pharmaceutical Sciences |
repository.name.fl_str_mv |
Brazilian Journal of Pharmaceutical Sciences - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
bjps@usp.br||elizabeth.igne@gmail.com |
_version_ |
1800222913349025792 |