Morphologic caracteristics of cells on the erytrocytic series of the Caiman crocodilus yacare (Daudin, 1802; Reptilia, crocodilia)
Autor(a) principal: | |
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Data de Publicação: | 2005 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Brazilian Journal of Veterinary Research and Animal Science |
Texto Completo: | https://www.revistas.usp.br/bjvras/article/view/26407 |
Resumo: | We studied hemopoietic cells in five young and healthy Brazilian cayman (Caiman crocodilus yacare). Peripheral blood samples (in EDTA) were collected by cervical venous sinuses puncture and bone marrow samples were obtained by femural bone needle aspiration. The bone marrow histological study was performed on femoral bone samples, with staining for HE, Giemsa and by Gomori technique for reticulin. Peripheral blood and marrow films were stained by Leishman or Rosenfeld technique. Active hemopoietic tissues were found by transversal section of the femur. Immature erythrocytic cells were found inside of sinusoid capilar vessels. The more immature erythrocytic cells were observed adhered to the vessel walls and the more mature ones were distributed through the central area. On marrow films, the erythrocytic immature cells showed a round shape with a fine nuclear chromatin pattern, while the mature cells were smaller, with an eliptic shape and a dense chromatin pattern. |
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Brazilian Journal of Veterinary Research and Animal Science |
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Morphologic caracteristics of cells on the erytrocytic series of the Caiman crocodilus yacare (Daudin, 1802; Reptilia, crocodilia)Aspectos morfológicos de células da série eritrocítica de Caiman crocodilus yacare (Daudin, 1802; Reptilia, crocodilia)Hemopoietic systemErytrocyteErythrocytic seriesBone marrowHematopoeseSérie eritrocíticaEritrócitosMedula ósseaWe studied hemopoietic cells in five young and healthy Brazilian cayman (Caiman crocodilus yacare). Peripheral blood samples (in EDTA) were collected by cervical venous sinuses puncture and bone marrow samples were obtained by femural bone needle aspiration. The bone marrow histological study was performed on femoral bone samples, with staining for HE, Giemsa and by Gomori technique for reticulin. Peripheral blood and marrow films were stained by Leishman or Rosenfeld technique. Active hemopoietic tissues were found by transversal section of the femur. Immature erythrocytic cells were found inside of sinusoid capilar vessels. The more immature erythrocytic cells were observed adhered to the vessel walls and the more mature ones were distributed through the central area. On marrow films, the erythrocytic immature cells showed a round shape with a fine nuclear chromatin pattern, while the mature cells were smaller, with an eliptic shape and a dense chromatin pattern.Com o objetivo de estudar o sistema hematopoético do réptil Caiman crocodilus yacare, foram utilizados cinco exemplares hígidos e jovens (aproximadamente um ano de idade), provenientes de São Paulo e de Mato Grosso do Sul. Amostras de sangue periférico foram colhidas em EDTA, através da punção do seio venoso cervical, e da medula óssea por punção aspirativa do osso femural. Estudo histológico da medula óssea femural foi realizado através da coloração por HE, Giemsa e técnica de Gomori para fibras reticulínicas. Os esfregaços de sangue e de medula foram processados para coloração panótica (Leishman ou Rosenfeld). A presença de sítios hematopoéticos ativos foi demonstrada histologicamente nos cortes transversais do fêmur, principalmente no terço distal. As células da linhagem eritrocítica foram encontradas dentro dos vasos capilares sinusóides medulares, na região central do osso. As células precursoras desta linhagem estavam aderidas à parede do vaso, e suas sucessoras distribuídas em direção ao centro do sinusóide à medida que amadureciam. Nos esfregaços de medula, as células imaturas da linhagem eritrocítica mostraram-se arredondadas e com núcleo predominantemente frouxo, em contraposição às células maduras, de núcleo condensado e forma em geral elíptica. Foram vistos pró-eritroblastos, eritroblastos basofílicos, policromáticos e ortocromáticos, e finalmente eritrócitos.Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia2005-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/bjvras/article/view/2640710.11606/issn.1678-4456.bjvras.2005.26407Brazilian Journal of Veterinary Research and Animal Science; Vol. 42 Núm. 5 (2005); 319-326Brazilian Journal of Veterinary Research and Animal Science; Vol. 42 No. 5 (2005); 319-326Brazilian Journal of Veterinary Research and Animal Science; v. 42 n. 5 (2005); 319-326Brazilian Journal of Veterinary Research and Animal Science; V. 42 N. 5 (2005); 319-3261678-44561413-9596reponame:Brazilian Journal of Veterinary Research and Animal Scienceinstname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)instacron:USPporhttps://www.revistas.usp.br/bjvras/article/view/26407/28190Moura, Weber Leal deCarvalho, Vinicius Antonio Lima deSantos, Antenor AguiarEgami, Mizue ImotoSilva, Maria ReginaYamamoto, Mihokoinfo:eu-repo/semantics/openAccess2020-06-23T04:19:36Zoai:revistas.usp.br:article/26407Revistahttps://www.revistas.usp.br/bjvrasPUBhttps://www.revistas.usp.br/bjvras/oaibjvras@usp.br1413-95961413-9596opendoar:https://www.revistas.usp.br/bjvras/index2023-01-12T16:42:42.131643Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)false |
dc.title.none.fl_str_mv |
Morphologic caracteristics of cells on the erytrocytic series of the Caiman crocodilus yacare (Daudin, 1802; Reptilia, crocodilia) Aspectos morfológicos de células da série eritrocítica de Caiman crocodilus yacare (Daudin, 1802; Reptilia, crocodilia) |
title |
Morphologic caracteristics of cells on the erytrocytic series of the Caiman crocodilus yacare (Daudin, 1802; Reptilia, crocodilia) |
spellingShingle |
Morphologic caracteristics of cells on the erytrocytic series of the Caiman crocodilus yacare (Daudin, 1802; Reptilia, crocodilia) Moura, Weber Leal de Hemopoietic system Erytrocyte Erythrocytic series Bone marrow Hematopoese Série eritrocítica Eritrócitos Medula óssea |
title_short |
Morphologic caracteristics of cells on the erytrocytic series of the Caiman crocodilus yacare (Daudin, 1802; Reptilia, crocodilia) |
title_full |
Morphologic caracteristics of cells on the erytrocytic series of the Caiman crocodilus yacare (Daudin, 1802; Reptilia, crocodilia) |
title_fullStr |
Morphologic caracteristics of cells on the erytrocytic series of the Caiman crocodilus yacare (Daudin, 1802; Reptilia, crocodilia) |
title_full_unstemmed |
Morphologic caracteristics of cells on the erytrocytic series of the Caiman crocodilus yacare (Daudin, 1802; Reptilia, crocodilia) |
title_sort |
Morphologic caracteristics of cells on the erytrocytic series of the Caiman crocodilus yacare (Daudin, 1802; Reptilia, crocodilia) |
author |
Moura, Weber Leal de |
author_facet |
Moura, Weber Leal de Carvalho, Vinicius Antonio Lima de Santos, Antenor Aguiar Egami, Mizue Imoto Silva, Maria Regina Yamamoto, Mihoko |
author_role |
author |
author2 |
Carvalho, Vinicius Antonio Lima de Santos, Antenor Aguiar Egami, Mizue Imoto Silva, Maria Regina Yamamoto, Mihoko |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Moura, Weber Leal de Carvalho, Vinicius Antonio Lima de Santos, Antenor Aguiar Egami, Mizue Imoto Silva, Maria Regina Yamamoto, Mihoko |
dc.subject.por.fl_str_mv |
Hemopoietic system Erytrocyte Erythrocytic series Bone marrow Hematopoese Série eritrocítica Eritrócitos Medula óssea |
topic |
Hemopoietic system Erytrocyte Erythrocytic series Bone marrow Hematopoese Série eritrocítica Eritrócitos Medula óssea |
description |
We studied hemopoietic cells in five young and healthy Brazilian cayman (Caiman crocodilus yacare). Peripheral blood samples (in EDTA) were collected by cervical venous sinuses puncture and bone marrow samples were obtained by femural bone needle aspiration. The bone marrow histological study was performed on femoral bone samples, with staining for HE, Giemsa and by Gomori technique for reticulin. Peripheral blood and marrow films were stained by Leishman or Rosenfeld technique. Active hemopoietic tissues were found by transversal section of the femur. Immature erythrocytic cells were found inside of sinusoid capilar vessels. The more immature erythrocytic cells were observed adhered to the vessel walls and the more mature ones were distributed through the central area. On marrow films, the erythrocytic immature cells showed a round shape with a fine nuclear chromatin pattern, while the mature cells were smaller, with an eliptic shape and a dense chromatin pattern. |
publishDate |
2005 |
dc.date.none.fl_str_mv |
2005-10-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/bjvras/article/view/26407 10.11606/issn.1678-4456.bjvras.2005.26407 |
url |
https://www.revistas.usp.br/bjvras/article/view/26407 |
identifier_str_mv |
10.11606/issn.1678-4456.bjvras.2005.26407 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/bjvras/article/view/26407/28190 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia |
publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia |
dc.source.none.fl_str_mv |
Brazilian Journal of Veterinary Research and Animal Science; Vol. 42 Núm. 5 (2005); 319-326 Brazilian Journal of Veterinary Research and Animal Science; Vol. 42 No. 5 (2005); 319-326 Brazilian Journal of Veterinary Research and Animal Science; v. 42 n. 5 (2005); 319-326 Brazilian Journal of Veterinary Research and Animal Science; V. 42 N. 5 (2005); 319-326 1678-4456 1413-9596 reponame:Brazilian Journal of Veterinary Research and Animal Science instname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP) instacron:USP |
instname_str |
Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Brazilian Journal of Veterinary Research and Animal Science |
collection |
Brazilian Journal of Veterinary Research and Animal Science |
repository.name.fl_str_mv |
Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP) |
repository.mail.fl_str_mv |
bjvras@usp.br |
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1797051557215207424 |