Molecular detection of Ehrlichia canis in Rhipicephalus sanguineus (s.l.) ticks in dogs and their domestic environment in Cuiaba, MT, Brazil

Detalhes bibliográficos
Autor(a) principal: Costa, Jackeliny Santos
Data de Publicação: 2019
Outros Autores: Melo, Andréia Lima Tome, Witter, Rute, Pacheco, Thabata Anjos, Chitarra, Cristiane Silva, Carvalho, Izabelle Thayná Soares, Nakazato, Luciano, Dutra, Valeria, Pacheco, Richard Campos, Aguiar, Daniel Moura de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Veterinary Research and Animal Science
Texto Completo: https://www.revistas.usp.br/bjvras/article/view/153661
Resumo: The central region of Brazil is known to be an endemic area for canine ehrlichiosis. Therefore, this study aims to determine the prevalence rates of E. canis infection in dogs and in Rhipicephalus sanguineus ticks collected from the dogs and their home environments. Serum samples and genomic DNA from the blood of 20 dogs and 299 ticks were analyzed by IFA and PCR assays in order to detect Ehrlichia canis antibodies and DNA. Nine (45%) of the 20 dogs were seropositive for E. canis, with titers ranging from 80 to 10240, and 6 dogs (30%) were positive for Ehrlichia spp. by PCR. Five free-living ticks were positive (2.89%, 95% confidence interval: 0.94-6.62%), as were six ticks attached to dogs (4.76%; 95% CI: 1.77-10.0%). The two groups showed a similar infection rate (P=0.395). Partial dsb DNA sequences of two samples from ticks were identical to each other and 100% (350/350 nucleotides) were identical to E. canis. Despite the high serological and molecular rates of canine ehrlichiosis in Cuiabá, the prevalence among infected ticks was lower than that found among dogs. However, adult ticks may remain infective much longer to ensure their infestation and infection of susceptible dogs.
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spelling Molecular detection of Ehrlichia canis in Rhipicephalus sanguineus (s.l.) ticks in dogs and their domestic environment in Cuiaba, MT, BrazilDetecção molecular de Ehrlichia canis em carrapatos Rhipicephalus sanguineus (s.l.), em cães e em seus ambientes domésticos em Cuiabá, MT, BrasilAnaplasmataceaeVectorPrevalencePCRAntibodyAnaplasmataceaeVetorPrevalênciaPCRAnticorpoThe central region of Brazil is known to be an endemic area for canine ehrlichiosis. Therefore, this study aims to determine the prevalence rates of E. canis infection in dogs and in Rhipicephalus sanguineus ticks collected from the dogs and their home environments. Serum samples and genomic DNA from the blood of 20 dogs and 299 ticks were analyzed by IFA and PCR assays in order to detect Ehrlichia canis antibodies and DNA. Nine (45%) of the 20 dogs were seropositive for E. canis, with titers ranging from 80 to 10240, and 6 dogs (30%) were positive for Ehrlichia spp. by PCR. Five free-living ticks were positive (2.89%, 95% confidence interval: 0.94-6.62%), as were six ticks attached to dogs (4.76%; 95% CI: 1.77-10.0%). The two groups showed a similar infection rate (P=0.395). Partial dsb DNA sequences of two samples from ticks were identical to each other and 100% (350/350 nucleotides) were identical to E. canis. Despite the high serological and molecular rates of canine ehrlichiosis in Cuiabá, the prevalence among infected ticks was lower than that found among dogs. However, adult ticks may remain infective much longer to ensure their infestation and infection of susceptible dogs.A região central do Brasil é caracterizada como uma área endêmica para erliquiose canina. Devido a isso, o presente estudo objetivou determinar a prevalência de infecção em cães e seus carrapatos R. sanguineus, coletados a partir do mesmo ambiente. As amostras de soro e DNA genômico de sangue de 20 cães e 299 carrapatos foram testadas por RIFI e PCR a fim de detectar anticorpos e DNA de Ehrlichia canis. Do total, 9 (45%) eram soropositivos para E. canis com títulos variando de 80 a 10240 e 6 cães (30%) positivos para Ehrlichia spp. por PCR. Cinco carrapatos de vida livre (2,89%; intervalo de confiança 95%: 0,94-6,62%), e 6 carrapatos fixados em cães (4,76%; IC 95%: 1,77-10,0%) foram positivos. A taxa de infecção foi semelhante entre ambos os grupos (P=0.395). As sequências parciais do gene dsb de 2 amostras de carrapatos foram idênticas entre si e 100% (350/350) idênticas à E. canis. Apesar das altas taxas sorológicas e moleculares de erliquiose canina em Cuiabá, a prevalência de carrapatos infectados foi menor que o encontrado emcães. Por outro lado, os carrapatos adultos podem permanecer infectados por tempo suficiente para garantir a infestação e infecção aos cães susceptíveis.Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia2019-09-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/bjvras/article/view/15366110.11606/issn.1678-4456.bjvras.2019.153661Brazilian Journal of Veterinary Research and Animal Science; Vol. 56 Núm. 2 (2019); e153661Brazilian Journal of Veterinary Research and Animal Science; Vol. 56 No. 2 (2019); e153661Brazilian Journal of Veterinary Research and Animal Science; v. 56 n. 2 (2019); e153661Brazilian Journal of Veterinary Research and Animal Science; V. 56 N. 2 (2019); e1536611678-44561413-9596reponame:Brazilian Journal of Veterinary Research and Animal Scienceinstname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)instacron:USPenghttps://www.revistas.usp.br/bjvras/article/view/153661/155952Copyright (c) 2019 Brazilian Journal of Veterinary Research and Animal Scienceinfo:eu-repo/semantics/openAccessCosta, Jackeliny SantosMelo, Andréia Lima TomeWitter, RutePacheco, Thabata AnjosChitarra, Cristiane SilvaCarvalho, Izabelle Thayná SoaresNakazato, LucianoDutra, ValeriaPacheco, Richard CamposAguiar, Daniel Moura de2020-06-23T04:02:21Zoai:revistas.usp.br:article/153661Revistahttps://www.revistas.usp.br/bjvrasPUBhttps://www.revistas.usp.br/bjvras/oaibjvras@usp.br1413-95961413-9596opendoar:https://www.revistas.usp.br/bjvras/index2023-01-12T16:44:03.399383Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)false
dc.title.none.fl_str_mv Molecular detection of Ehrlichia canis in Rhipicephalus sanguineus (s.l.) ticks in dogs and their domestic environment in Cuiaba, MT, Brazil
Detecção molecular de Ehrlichia canis em carrapatos Rhipicephalus sanguineus (s.l.), em cães e em seus ambientes domésticos em Cuiabá, MT, Brasil
title Molecular detection of Ehrlichia canis in Rhipicephalus sanguineus (s.l.) ticks in dogs and their domestic environment in Cuiaba, MT, Brazil
spellingShingle Molecular detection of Ehrlichia canis in Rhipicephalus sanguineus (s.l.) ticks in dogs and their domestic environment in Cuiaba, MT, Brazil
Costa, Jackeliny Santos
Anaplasmataceae
Vector
Prevalence
PCR
Antibody
Anaplasmataceae
Vetor
Prevalência
PCR
Anticorpo
title_short Molecular detection of Ehrlichia canis in Rhipicephalus sanguineus (s.l.) ticks in dogs and their domestic environment in Cuiaba, MT, Brazil
title_full Molecular detection of Ehrlichia canis in Rhipicephalus sanguineus (s.l.) ticks in dogs and their domestic environment in Cuiaba, MT, Brazil
title_fullStr Molecular detection of Ehrlichia canis in Rhipicephalus sanguineus (s.l.) ticks in dogs and their domestic environment in Cuiaba, MT, Brazil
title_full_unstemmed Molecular detection of Ehrlichia canis in Rhipicephalus sanguineus (s.l.) ticks in dogs and their domestic environment in Cuiaba, MT, Brazil
title_sort Molecular detection of Ehrlichia canis in Rhipicephalus sanguineus (s.l.) ticks in dogs and their domestic environment in Cuiaba, MT, Brazil
author Costa, Jackeliny Santos
author_facet Costa, Jackeliny Santos
Melo, Andréia Lima Tome
Witter, Rute
Pacheco, Thabata Anjos
Chitarra, Cristiane Silva
Carvalho, Izabelle Thayná Soares
Nakazato, Luciano
Dutra, Valeria
Pacheco, Richard Campos
Aguiar, Daniel Moura de
author_role author
author2 Melo, Andréia Lima Tome
Witter, Rute
Pacheco, Thabata Anjos
Chitarra, Cristiane Silva
Carvalho, Izabelle Thayná Soares
Nakazato, Luciano
Dutra, Valeria
Pacheco, Richard Campos
Aguiar, Daniel Moura de
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Costa, Jackeliny Santos
Melo, Andréia Lima Tome
Witter, Rute
Pacheco, Thabata Anjos
Chitarra, Cristiane Silva
Carvalho, Izabelle Thayná Soares
Nakazato, Luciano
Dutra, Valeria
Pacheco, Richard Campos
Aguiar, Daniel Moura de
dc.subject.por.fl_str_mv Anaplasmataceae
Vector
Prevalence
PCR
Antibody
Anaplasmataceae
Vetor
Prevalência
PCR
Anticorpo
topic Anaplasmataceae
Vector
Prevalence
PCR
Antibody
Anaplasmataceae
Vetor
Prevalência
PCR
Anticorpo
description The central region of Brazil is known to be an endemic area for canine ehrlichiosis. Therefore, this study aims to determine the prevalence rates of E. canis infection in dogs and in Rhipicephalus sanguineus ticks collected from the dogs and their home environments. Serum samples and genomic DNA from the blood of 20 dogs and 299 ticks were analyzed by IFA and PCR assays in order to detect Ehrlichia canis antibodies and DNA. Nine (45%) of the 20 dogs were seropositive for E. canis, with titers ranging from 80 to 10240, and 6 dogs (30%) were positive for Ehrlichia spp. by PCR. Five free-living ticks were positive (2.89%, 95% confidence interval: 0.94-6.62%), as were six ticks attached to dogs (4.76%; 95% CI: 1.77-10.0%). The two groups showed a similar infection rate (P=0.395). Partial dsb DNA sequences of two samples from ticks were identical to each other and 100% (350/350 nucleotides) were identical to E. canis. Despite the high serological and molecular rates of canine ehrlichiosis in Cuiabá, the prevalence among infected ticks was lower than that found among dogs. However, adult ticks may remain infective much longer to ensure their infestation and infection of susceptible dogs.
publishDate 2019
dc.date.none.fl_str_mv 2019-09-09
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/bjvras/article/view/153661
10.11606/issn.1678-4456.bjvras.2019.153661
url https://www.revistas.usp.br/bjvras/article/view/153661
identifier_str_mv 10.11606/issn.1678-4456.bjvras.2019.153661
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/bjvras/article/view/153661/155952
dc.rights.driver.fl_str_mv Copyright (c) 2019 Brazilian Journal of Veterinary Research and Animal Science
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2019 Brazilian Journal of Veterinary Research and Animal Science
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia
publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia
dc.source.none.fl_str_mv Brazilian Journal of Veterinary Research and Animal Science; Vol. 56 Núm. 2 (2019); e153661
Brazilian Journal of Veterinary Research and Animal Science; Vol. 56 No. 2 (2019); e153661
Brazilian Journal of Veterinary Research and Animal Science; v. 56 n. 2 (2019); e153661
Brazilian Journal of Veterinary Research and Animal Science; V. 56 N. 2 (2019); e153661
1678-4456
1413-9596
reponame:Brazilian Journal of Veterinary Research and Animal Science
instname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)
instacron:USP
instname_str Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)
instacron_str USP
institution USP
reponame_str Brazilian Journal of Veterinary Research and Animal Science
collection Brazilian Journal of Veterinary Research and Animal Science
repository.name.fl_str_mv Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)
repository.mail.fl_str_mv bjvras@usp.br
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